FLGL - ECOLI - GREMLIN Contacts

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FLGL - Flagellar hook-associated protein 3

UniProt:	P29744	Sequences: MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPIAASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTLSDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESIKQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADSEADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMSDLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLSK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG11545
Length:	317 (300)
Sequences:	5402 (4183)
Seq/√Len:	241.5

ECOLI - FLGL FLIC
BACSU - FLGL FLA

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
91_Q	237_K	4.836	1.00
83_V	121_L	3.575	1.00
111_A	115_Q	3.344	1.00
90_A	114_I	3.250	1.00
276_S	280_D	3.183	1.00
89_N	113_D	3.116	1.00
35_R	42_D	2.905	1.00
29_Q	286_T	2.794	1.00
285_A	289_S	2.752	1.00
63_T	265_S	2.715	1.00
103_S	106_D	2.665	1.00
86_A	121_L	2.643	1.00
86_A	117_L	2.635	1.00
53_S	275_M	2.520	1.00
129_D	134_Y	2.497	1.00
116_G	119_D	2.455	1.00
34_K	279_V	2.450	1.00
89_N	93_K	2.405	1.00
60_S	268_A	2.391	1.00
275_M	279_V	2.373	1.00
36_V	45_A	2.355	1.00
19_S	300_S	2.329	1.00
120_Q	123_N	2.322	1.00
281_V	286_T	2.320	1.00
73_V	250_L	2.202	1.00
26_Y	293_Q	2.192	1.00
115_Q	212_K	2.142	1.00
66_R	261_D	2.128	1.00
104_D	108_A	2.098	1.00
77_E	247_R	2.082	1.00
25_K	29_Q	2.063	1.00
61_Q	64_L	2.053	1.00
145_P	148_E	2.036	1.00
90_A	117_L	1.943	1.00
93_K	113_D	1.914	1.00
53_S	279_V	1.912	1.00
57_A	92_E	1.908	1.00
28_E	38_N	1.900	1.00
146_F	149_E	1.877	1.00
237_K	241_N	1.858	1.00
94_I	233_N	1.846	1.00
98_S	230_D	1.839	1.00
45_A	278_L	1.819	1.00
82_Q	85_T	1.809	1.00
108_A	112_T	1.798	1.00
89_N	92_E	1.749	1.00
246_V	249_E	1.743	1.00
93_K	110_L	1.728	1.00
110_L	223_E	1.720	1.00
145_P	149_E	1.697	1.00
83_V	243_V	1.690	1.00
102_L	106_D	1.686	1.00
87_I	236_L	1.670	1.00
291_I	294_Q	1.641	1.00
87_I	243_V	1.622	1.00
49_A	279_V	1.620	1.00
83_V	86_A	1.609	1.00
100_G	107_R	1.583	1.00
30_M	286_T	1.578	1.00
87_I	240_L	1.568	1.00
90_A	236_L	1.541	1.00
14_R	18_N	1.537	1.00
140_K	143_T	1.518	1.00
70_T	258_E	1.514	1.00
42_D	45_A	1.503	1.00
59_N	264_G	1.502	1.00
202_A	206_S	1.497	1.00
79_V	124_L	1.494	1.00
26_Y	289_S	1.462	1.00
105_D	108_A	1.461	1.00
110_L	114_I	1.444	1.00
115_Q	119_D	1.436	1.00
227_A	231_K	1.432	1.00
97_A	110_L	1.428	1.00
86_A	124_L	1.413	1.00
115_Q	118_R	1.388	1.00
91_Q	95_V	1.388	1.00
106_D	109_S	1.381	1.00
96_Y	110_L	1.380	1.00
80_L	243_V	1.375	1.00
88_Q	91_Q	1.371	1.00
109_S	112_T	1.350	1.00
100_G	226_A	1.347	1.00
222_K	228_A	1.346	1.00
82_Q	124_L	1.336	1.00
205_D	209_A	1.329	1.00
86_A	120_Q	1.324	1.00
56_Q	272_T	1.319	1.00
144_A	147_S	1.319	1.00
36_V	49_A	1.316	1.00
111_A	114_I	1.308	1.00
268_A	272_T	1.299	1.00
83_V	124_L	1.272	1.00
84_T	240_L	1.270	1.00
161_K	169_S	1.270	1.00
36_V	41_D	1.266	1.00
199_N	202_A	1.255	1.00
28_E	37_V	1.240	1.00
28_E	32_T	1.237	1.00
112_T	249_E	1.228	1.00
66_R	257_L	1.226	1.00
70_T	261_D	1.223	1.00
80_L	83_V	1.217	1.00
95_V	98_S	1.194	1.00
56_Q	271_Q	1.189	1.00
26_Y	286_T	1.187	1.00
143_T	146_F	1.184	1.00
67_T	261_D	1.180	1.00
22_E	293_Q	1.177	1.00
105_D	241_N	1.177	1.00
302_K	306_D	1.173	1.00
273_Q	277_D	1.166	1.00
283_W	287_I	1.162	1.00
112_T	245_T	1.130	1.00
81_S	85_T	1.128	1.00
290_Y	294_Q	1.125	1.00
126_N	137_A	1.125	1.00
61_Q	92_E	1.124	1.00
94_I	207_A	1.124	1.00
34_K	281_V	1.109	1.00
287_I	291_I	1.109	1.00
25_K	28_E	1.101	1.00
197_E	202_A	1.096	1.00
8_M	24_M	1.096	1.00
95_V	233_N	1.095	1.00
163_Q	167_S	1.091	1.00
102_L	110_L	1.085	1.00
11_Q	14_R	1.081	1.00
122_L	126_N	1.080	1.00
79_V	128_T	1.078	1.00
26_Y	281_V	1.074	1.00
118_R	122_L	1.072	1.00
59_N	268_A	1.071	1.00
84_T	244_L	1.070	1.00
66_R	264_G	1.068	1.00
61_Q	65_A	1.066	1.00
231_K	235_G	1.052	1.00
23_W	27_G	1.046	1.00
63_T	264_G	1.040	1.00
225_A	228_A	1.037	1.00
226_A	230_D	1.034	1.00
88_Q	240_L	1.031	1.00
50_V	53_S	1.030	1.00
118_R	205_D	1.027	1.00
115_Q	208_I	1.020	1.00
249_E	253_Q	1.016	1.00
84_T	243_V	1.014	1.00
18_N	22_E	1.007	1.00
212_K	215_V	1.001	1.00
175_T	178_K	0.997	1.00
208_I	211_L	0.997	1.00
19_S	23_W	0.995	1.00
93_K	96_Y	0.989	1.00
32_T	37_V	0.988	1.00
7_M	11_Q	0.987	1.00
287_I	290_Y	0.985	1.00
238_N	241_N	0.982	1.00
70_T	74_S	0.982	1.00
88_Q	92_E	0.978	1.00
49_A	275_M	0.976	1.00
94_I	229_L	0.976	1.00
90_A	118_R	0.975	1.00
234_R	238_N	0.974	1.00
262_S	265_S	0.973	1.00
59_N	63_T	0.965	1.00
125_A	136_F	0.963	1.00
297_L	301_Y	0.961	1.00
241_N	245_T	0.958	1.00
9_Y	13_M	0.955	1.00
16_I	297_L	0.954	1.00
234_R	237_K	0.949	1.00
114_I	223_E	0.948	1.00
134_Y	137_A	0.947	1.00
129_D	132_G	0.944	1.00
81_S	247_R	0.942	1.00
85_T	88_Q	0.941	1.00
294_Q	298_Q	0.939	1.00
117_L	120_Q	0.937	1.00
60_S	63_T	0.936	1.00
82_Q	259_S	0.918	1.00
51_V	106_D	0.917	1.00
53_S	57_A	0.914	1.00
99_N	102_L	0.913	1.00
134_Y	145_P	0.913	1.00
223_E	229_L	0.910	1.00
14_R	269_L	0.909	1.00
301_Y	311_S	0.901	1.00
84_T	88_Q	0.896	1.00
98_S	229_L	0.888	1.00
64_L	68_F	0.882	0.99
89_N	117_L	0.881	0.99
24_M	27_G	0.877	0.99
210_A	215_V	0.877	0.99
116_G	253_Q	0.877	0.99
94_I	98_S	0.868	0.99
74_S	254_L	0.867	0.99
225_A	229_L	0.866	0.99
153_Y	156_G	0.858	0.99
20_Q	297_L	0.854	0.99
139_Y	156_G	0.850	0.99
4_S	34_K	0.846	0.99
176_G	180_F	0.844	0.99
69_A	257_L	0.844	0.99
23_W	26_Y	0.842	0.99
163_Q	166_A	0.836	0.99
238_N	242_N	0.834	0.99
13_M	17_T	0.833	0.99
170_M	174_H	0.831	0.99
19_S	22_E	0.831	0.99
15_G	32_T	0.830	0.99
206_S	209_A	0.825	0.99
165_D	168_R	0.823	0.99
164_V	260_L	0.821	0.99
123_N	127_T	0.820	0.99
137_A	145_P	0.817	0.99
19_S	293_Q	0.816	0.99
94_I	236_L	0.815	0.99
294_Q	306_D	0.815	0.99
245_T	249_E	0.815	0.99
63_T	67_T	0.813	0.99
208_I	212_K	0.811	0.99
95_V	237_K	0.807	0.99
13_M	288_S	0.803	0.99
18_N	21_A	0.801	0.99
144_A	148_E	0.798	0.99
289_S	292_M	0.796	0.99
97_A	229_L	0.789	0.99
310_L	315_L	0.787	0.99
70_T	254_L	0.786	0.99
286_T	289_S	0.786	0.99
62_Y	264_G	0.785	0.99
91_Q	233_N	0.782	0.99
164_V	167_S	0.782	0.99
90_A	204_L	0.782	0.99
260_L	264_G	0.781	0.99
27_G	290_Y	0.781	0.99
4_S	7_M	0.780	0.99
153_Y	157_A	0.779	0.99
137_A	143_T	0.777	0.99
22_E	289_S	0.777	0.99
120_Q	256_E	0.771	0.99
29_Q	281_V	0.769	0.99
17_T	20_Q	0.766	0.99
77_E	250_L	0.764	0.99
94_I	232_T	0.759	0.98
63_T	268_A	0.754	0.98
200_L	204_L	0.753	0.98
84_T	247_R	0.751	0.98
152_K	155_G	0.750	0.98
16_I	300_S	0.746	0.98
102_L	107_R	0.745	0.98
122_L	125_A	0.737	0.98
254_L	258_E	0.734	0.98
96_Y	102_L	0.734	0.98
98_S	233_N	0.730	0.98
311_S	314_Q	0.728	0.98
57_A	60_S	0.726	0.98
231_K	234_R	0.726	0.98
164_V	170_M	0.724	0.98
73_V	257_L	0.722	0.98
128_T	132_G	0.722	0.98
22_E	25_K	0.722	0.98
69_A	172_I	0.720	0.98
144_A	149_E	0.717	0.98
160_I	165_D	0.716	0.98
198_T	206_S	0.715	0.98
44_I	48_Q	0.706	0.98
143_T	147_S	0.706	0.98
224_T	228_A	0.705	0.98
261_D	265_S	0.705	0.98
35_R	64_L	0.700	0.97
265_S	268_A	0.699	0.97
93_K	117_L	0.698	0.97
16_I	301_Y	0.696	0.97
134_Y	144_A	0.694	0.97
143_T	148_E	0.694	0.97
266_D	269_L	0.694	0.97
203_M	206_S	0.694	0.97
267_R	271_Q	0.693	0.97
258_E	262_S	0.693	0.97
199_N	203_M	0.692	0.97
205_D	208_I	0.690	0.97
228_A	232_T	0.690	0.97
56_Q	275_M	0.689	0.97
168_R	172_I	0.685	0.97
168_R	171_V	0.683	0.97
310_L	314_Q	0.681	0.97
37_V	41_D	0.678	0.97
46_A	50_V	0.672	0.97
169_S	172_I	0.672	0.97
209_A	213_T	0.670	0.97
174_H	180_F	0.662	0.96
79_V	136_F	0.661	0.96
26_Y	29_Q	0.661	0.96
137_A	140_K	0.661	0.96
90_A	121_L	0.657	0.96
114_I	118_R	0.654	0.96
111_A	118_R	0.654	0.96
71_Q	74_S	0.654	0.96
162_Q	165_D	0.653	0.96
8_M	27_G	0.652	0.96
206_S	211_L	0.652	0.96
94_I	114_I	0.650	0.96
10_Q	14_R	0.649	0.96
19_S	297_L	0.648	0.96
192_D	197_E	0.645	0.96
276_S	279_V	0.643	0.96
118_R	121_L	0.643	0.96
168_R	176_G	0.643	0.96
76_E	79_V	0.642	0.96
299_A	302_K	0.639	0.96
8_M	12_N	0.638	0.96
52_L	278_L	0.637	0.96
9_Y	12_N	0.636	0.95
178_K	182_S	0.634	0.95
88_Q	95_V	0.629	0.95
171_V	174_H	0.628	0.95
147_S	154_V	0.628	0.95
21_A	25_K	0.622	0.95
294_Q	301_Y	0.622	0.95
304_F	308_Q	0.622	0.95
206_S	212_K	0.622	0.95
302_K	314_Q	0.621	0.95
310_L	313_F	0.621	0.95
140_K	145_P	0.620	0.95
76_E	174_H	0.619	0.95
201_F	211_L	0.619	0.95
12_N	304_F	0.618	0.95
117_L	259_S	0.617	0.95
300_S	303_A	0.617	0.95
221_D	232_T	0.616	0.95
52_L	56_Q	0.614	0.95
79_V	83_V	0.611	0.94
163_Q	169_S	0.611	0.94
79_V	127_T	0.611	0.94
289_S	293_Q	0.610	0.94
66_R	260_L	0.608	0.94
285_A	288_S	0.601	0.94
35_R	45_A	0.600	0.94
177_D	181_D	0.600	0.94
142_E	146_F	0.600	0.94
112_T	115_Q	0.598	0.94
291_I	295_T	0.597	0.94
301_Y	304_F	0.597	0.94
5_T	275_M	0.597	0.94
223_E	228_A	0.597	0.94
118_R	221_D	0.596	0.94
24_M	28_E	0.593	0.93
23_W	294_Q	0.592	0.93
15_G	28_E	0.591	0.93
266_D	270_G	0.591	0.93
138_G	145_P	0.591	0.93
210_A	232_T	0.590	0.93
129_D	133_R	0.588	0.93
113_D	252_T	0.587	0.93
75_L	79_V	0.583	0.93
298_Q	302_K	0.582	0.93
303_A	306_D	0.582	0.93
177_D	180_F	0.582	0.93
176_G	183_I	0.581	0.93
207_A	211_L	0.581	0.93
165_D	169_S	0.579	0.93
50_V	279_V	0.579	0.93
220_A	223_E	0.578	0.93
80_L	247_R	0.576	0.92
292_M	295_T	0.575	0.92
162_Q	172_I	0.575	0.92
174_H	179_I	0.573	0.92
206_S	210_A	0.573	0.92
290_Y	297_L	0.572	0.92
170_M	176_G	0.572	0.92
290_Y	306_D	0.569	0.92
103_S	109_S	0.568	0.92
202_A	205_D	0.568	0.92
202_A	211_L	0.567	0.92
50_V	244_L	0.567	0.92
48_Q	51_V	0.567	0.92
7_M	10_Q	0.566	0.92
73_V	253_Q	0.566	0.92
230_D	234_R	0.566	0.92
229_L	232_T	0.564	0.92
145_P	155_G	0.564	0.92
113_D	117_L	0.560	0.91
34_K	280_D	0.558	0.91
203_M	207_A	0.555	0.91
98_S	102_L	0.552	0.91
14_R	17_T	0.552	0.91
200_L	203_M	0.552	0.91
202_A	214_P	0.551	0.91
169_S	173_G	0.551	0.91
201_F	204_L	0.550	0.91
116_G	256_E	0.550	0.91
201_F	205_D	0.549	0.91
181_D	184_T	0.549	0.91
142_E	147_S	0.549	0.91
202_A	210_A	0.547	0.90
168_R	179_I	0.545	0.90
63_T	261_D	0.543	0.90
30_M	287_I	0.543	0.90
292_M	296_A	0.542	0.90
47_S	131_N	0.541	0.90
76_E	250_L	0.540	0.90
161_K	171_V	0.539	0.90
160_I	169_S	0.539	0.90
85_T	89_N	0.539	0.90
192_D	202_A	0.539	0.90
207_A	210_A	0.538	0.90
25_K	41_D	0.535	0.89
83_V	87_I	0.534	0.89
85_T	262_S	0.534	0.89
46_A	247_R	0.533	0.89
168_R	173_G	0.532	0.89
242_N	245_T	0.531	0.89
172_I	176_G	0.531	0.89
9_Y	288_S	0.530	0.89
146_F	153_Y	0.529	0.89
83_V	136_F	0.529	0.89
78_S	82_Q	0.527	0.89
140_K	156_G	0.527	0.89
60_S	285_A	0.525	0.89
297_L	300_S	0.524	0.88
174_H	178_K	0.523	0.88
295_T	310_L	0.522	0.88
304_F	313_F	0.521	0.88
40_S	73_V	0.520	0.88
302_K	305_T	0.519	0.88
211_L	214_P	0.518	0.88
232_T	235_G	0.518	0.88
133_R	143_T	0.518	0.88
201_F	212_K	0.518	0.88
117_L	121_L	0.516	0.88
174_H	177_D	0.516	0.88
73_V	254_L	0.515	0.88
306_D	316_S	0.514	0.88
142_E	145_P	0.513	0.87
176_G	179_I	0.512	0.87
42_D	64_L	0.512	0.87
60_S	272_T	0.512	0.87
107_R	229_L	0.511	0.87
168_R	177_D	0.510	0.87
198_T	202_A	0.510	0.87
47_S	109_S	0.510	0.87
97_A	107_R	0.509	0.87
121_L	127_T	0.509	0.87
12_N	15_G	0.509	0.87
46_A	49_A	0.507	0.87
140_K	146_F	0.504	0.86
67_T	277_D	0.503	0.86
233_N	237_K	0.502	0.86
98_S	101_T	0.500	0.86

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1ucuA	1	0.9811	100	0.312	Contact Map
2d4xA	1	0.6751	100	0.374	Contact Map
3pwxA	2	0.7003	100	0.388	Contact Map
3k8wA	1	0.7224	100	0.479	Contact Map
4nx9A	1	0.6498	100	0.591	Contact Map
3v47C	1	0.5804	99.9	0.63	Contact Map
4cfiA	1	0.5773	99.9	0.671	Contact Map
1io1A	1	0.6909	99.9	0.675	Contact Map
3k1iC	1	0.3785	99.3	0.789	Contact Map
1oryB	2	0.1199	96.9	0.889	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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