FENR - ECOLI - GREMLIN Contacts

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FENR - Ferredoxin--NADP reductase

UniProt:	P28861	Sequences: MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIDGERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDEVPHCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEKRYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESTIGLPMNKETSHVMLCGNPQMVRDTQQLLKETRQMTKHLRRRPGHMTAEHYW	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG11518
Length:	248 (219)
Sequences:	19324 (13592)
Seq/√Len:	918.4

ECOLI - FENR PAAE FRE HMP YEAX HCR CYSJ
BACSU - HMP PYRK CYSJ CYPD CYPE
HALSA - Q9HRZ4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
108_T	136_N	3.226	1.00
10_T	25_H	2.949	1.00
23_T	62_D	2.820	1.00
12_V	20_F	2.731	1.00
6_T	87_E	2.611	1.00
152_L	156_Q	2.598	1.00
127_L	131_L	2.533	1.00
21_S	64_E	2.532	1.00
138_V	169_Q	2.496	1.00
6_T	89_Q	2.459	1.00
11_K	23_T	2.443	1.00
83_K	86_D	2.412	1.00
23_T	64_E	2.400	1.00
15_W	21_S	2.383	1.00
113_A	121_Y	2.313	1.00
138_V	167_R	2.289	1.00
8_K	25_H	2.234	1.00
14_N	20_F	2.204	1.00
8_K	87_E	2.175	1.00
38_K	51_A	2.149	1.00
126_Q	154_L	2.148	1.00
40_G	89_Q	2.078	1.00
9_V	82_L	1.994	1.00
13_Q	21_S	1.971	1.00
110_W	209_V	1.967	1.00
114_T	214_N	1.956	1.00
7_G	90_V	1.947	1.00
69_T	80_A	1.919	1.00
55_V	66_Y	1.808	1.00
149_L	172_V	1.799	1.00
157_E	161_R	1.783	1.00
118_I	139_L	1.751	1.00
187_A	190_E	1.738	1.00
136_N	167_R	1.721	1.00
37_T	65_F	1.713	1.00
209_V	225_L	1.702	1.00
11_K	84_P	1.660	1.00
146_A	175_E	1.652	1.00
10_T	62_D	1.628	1.00
113_A	141_H	1.602	1.00
222_Q	226_K	1.563	1.00
145_Y	148_D	1.550	1.00
209_V	231_M	1.514	1.00
108_T	138_V	1.500	1.00
143_A	149_L	1.495	1.00
17_D	147_A	1.481	1.00
22_L	79_L	1.468	1.00
25_H	62_D	1.463	1.00
107_E	208_H	1.462	1.00
190_E	227_E	1.438	1.00
226_K	233_K	1.420	1.00
37_T	90_V	1.409	1.00
69_T	77_P	1.408	1.00
24_V	65_F	1.403	1.00
211_L	221_T	1.387	1.00
108_T	207_S	1.380	1.00
190_E	193_E	1.374	1.00
142_A	217_M	1.374	1.00
107_E	206_T	1.366	1.00
7_G	24_V	1.358	1.00
122_L	154_L	1.347	1.00
187_A	191_S	1.328	1.00
112_L	221_T	1.327	1.00
112_L	217_M	1.312	1.00
122_L	126_Q	1.292	1.00
13_Q	23_T	1.286	1.00
114_T	217_M	1.282	1.00
38_K	91_V	1.251	1.00
37_T	54_Y	1.250	1.00
142_A	173_S	1.241	1.00
122_L	151_Y	1.227	1.00
146_A	152_L	1.215	1.00
111_M	121_Y	1.215	1.00
156_Q	159_E	1.214	1.00
162_Y	165_K	1.211	1.00
149_L	170_T	1.206	1.00
66_Y	151_Y	1.197	1.00
129_K	161_R	1.197	1.00
224_L	227_E	1.173	1.00
8_K	27_P	1.166	1.00
143_A	170_T	1.151	1.00
9_V	24_V	1.150	1.00
77_P	80_A	1.148	1.00
19_L	116_T	1.147	1.00
178_A	182_T	1.143	1.00
215_P	219_R	1.139	1.00
12_V	22_L	1.136	1.00
211_L	218_V	1.130	1.00
27_P	87_E	1.126	1.00
12_V	84_P	1.126	1.00
128_G	137_L	1.120	1.00
155_M	168_I	1.112	1.00
66_Y	150_S	1.112	1.00
208_H	237_R	1.105	1.00
35_Q	96_G	1.102	1.00
21_S	151_Y	1.100	1.00
21_S	66_Y	1.091	1.00
111_M	137_L	1.088	1.00
121_Y	139_L	1.088	1.00
154_L	157_E	1.081	1.00
59_D	130_D	1.079	1.00
40_G	49_Q	1.069	1.00
73_G	76_S	1.065	1.00
223_Q	227_E	1.064	1.00
7_G	26_A	1.064	1.00
18_A	148_D	1.063	1.00
140_V	169_Q	1.061	1.00
57_S	126_Q	1.050	1.00
149_L	152_L	1.045	1.00
110_W	207_S	1.037	1.00
113_A	139_L	1.037	1.00
159_E	162_Y	1.033	1.00
82_L	86_D	1.032	1.00
139_L	166_L	1.027	1.00
216_Q	220_D	1.015	1.00
156_Q	170_T	1.013	1.00
129_K	162_Y	1.010	1.00
232_T	235_L	1.004	1.00
111_M	124_I	1.003	1.00
6_T	27_P	1.001	1.00
32_T	99_V	0.992	1.00
220_D	224_L	0.988	1.00
19_L	150_S	0.987	1.00
9_V	22_L	0.983	1.00
144_R	173_S	0.979	1.00
41_L	75_L	0.979	1.00
152_L	168_I	0.974	1.00
33_A	127_L	0.966	1.00
157_E	160_K	0.966	1.00
146_A	172_V	0.959	1.00
220_D	223_Q	0.954	1.00
125_L	137_L	0.950	1.00
116_T	119_G	0.946	1.00
145_Y	174_R	0.936	1.00
156_Q	160_K	0.932	1.00
18_A	68_V	0.931	1.00
12_V	83_K	0.917	1.00
92_S	95_A	0.916	1.00
39_L	65_F	0.914	1.00
16_T	148_D	0.905	1.00
175_E	178_A	0.904	1.00
118_I	155_M	0.900	1.00
78_R	81_A	0.888	1.00
10_T	23_T	0.881	1.00
144_R	174_R	0.880	1.00
56_N	126_Q	0.874	1.00
48_V	74_K	0.861	1.00
208_H	235_L	0.858	1.00
219_R	223_Q	0.858	1.00
188_L	191_S	0.857	1.00
125_L	155_M	0.843	1.00
107_E	135_K	0.840	1.00
143_A	148_D	0.840	1.00
100_L	124_I	0.839	1.00
17_D	148_D	0.838	1.00
159_E	163_E	0.833	1.00
211_L	236_R	0.833	1.00
109_L	137_L	0.831	1.00
60_N	126_Q	0.817	1.00
141_H	150_S	0.816	1.00
9_V	12_V	0.812	1.00
100_L	109_L	0.803	1.00
153_P	157_E	0.803	1.00
140_V	192_G	0.801	1.00
142_A	189_I	0.798	1.00
224_L	229_R	0.798	1.00
223_Q	226_K	0.795	1.00
151_Y	154_L	0.791	1.00
125_L	158_L	0.786	1.00
112_L	209_V	0.785	1.00
140_V	171_V	0.783	1.00
107_E	235_L	0.781	1.00
112_L	140_V	0.780	1.00
142_A	171_V	0.776	1.00
222_Q	236_R	0.774	1.00
153_P	156_Q	0.774	1.00
162_Y	166_L	0.773	1.00
18_A	71_P	0.772	1.00
55_V	64_E	0.768	1.00
203_N	207_S	0.765	1.00
40_G	47_R	0.761	1.00
159_E	166_L	0.759	1.00
42_E	47_R	0.756	1.00
129_K	158_L	0.756	1.00
109_L	128_G	0.754	1.00
188_L	192_G	0.751	1.00
48_V	75_L	0.750	1.00
183_G	188_L	0.750	1.00
79_L	82_L	0.748	1.00
91_V	95_A	0.747	1.00
154_L	158_L	0.744	1.00
185_I	188_L	0.742	1.00
145_Y	175_E	0.742	1.00
31_F	57_S	0.742	1.00
152_L	170_T	0.742	1.00
184_R	188_L	0.741	1.00
52_Y	76_S	0.739	1.00
38_K	49_Q	0.737	1.00
59_D	127_L	0.737	1.00
185_I	189_I	0.733	1.00
125_L	139_L	0.729	1.00
35_Q	99_V	0.728	1.00
31_F	58_P	0.725	1.00
72_D	77_P	0.725	1.00
118_I	151_Y	0.723	1.00
143_A	172_V	0.712	1.00
25_H	61_P	0.710	1.00
67_L	79_L	0.710	1.00
203_N	206_T	0.710	1.00
221_T	224_L	0.708	1.00
31_F	56_N	0.707	1.00
57_S	130_D	0.706	1.00
40_G	91_V	0.704	1.00
56_N	64_E	0.702	1.00
127_L	130_D	0.701	1.00
109_L	135_K	0.697	1.00
75_L	79_L	0.695	1.00
106_C	109_L	0.692	1.00
57_S	60_N	0.692	1.00
211_L	222_Q	0.690	1.00
111_M	125_L	0.689	1.00
19_L	66_Y	0.687	1.00
31_F	54_Y	0.686	1.00
125_L	166_L	0.682	1.00
204_K	207_S	0.682	1.00
159_E	168_I	0.681	1.00
7_G	63_L	0.679	1.00
114_T	216_Q	0.678	1.00
124_I	210_M	0.674	1.00
189_I	193_E	0.673	1.00
193_E	233_K	0.670	1.00
32_T	35_Q	0.665	1.00
231_M	236_R	0.665	1.00
70_V	73_G	0.662	1.00
183_G	187_A	0.657	1.00
11_K	21_S	0.656	1.00
186_P	190_E	0.656	1.00
78_R	82_L	0.655	1.00
66_Y	119_G	0.652	1.00
178_A	183_G	0.651	1.00
176_T	183_G	0.650	1.00
26_A	29_L	0.643	1.00
42_E	46_E	0.642	1.00
7_G	27_P	0.640	1.00
60_N	130_D	0.638	1.00
98_F	101_D	0.637	1.00
20_F	69_T	0.633	1.00
53_S	121_Y	0.632	1.00
159_E	167_R	0.629	1.00
106_C	134_F	0.627	1.00
190_E	224_L	0.627	1.00
108_T	135_K	0.626	1.00
109_L	208_H	0.625	1.00
108_T	206_T	0.625	1.00
111_M	210_M	0.622	1.00
24_V	88_V	0.621	1.00
49_Q	91_V	0.621	1.00
74_K	77_P	0.615	1.00
39_L	79_L	0.614	1.00
216_Q	219_R	0.611	1.00
101_D	208_H	0.608	1.00
114_T	220_D	0.605	1.00
43_I	46_E	0.603	1.00
38_K	95_A	0.603	1.00
206_T	235_L	0.602	1.00
29_L	92_S	0.601	1.00
102_E	131_L	0.601	1.00
219_R	222_Q	0.600	1.00
129_K	137_L	0.600	1.00
110_W	138_V	0.596	1.00
71_P	77_P	0.594	1.00
172_V	175_E	0.594	1.00
129_K	166_L	0.592	1.00
14_N	17_D	0.591	1.00
46_E	74_K	0.588	1.00
54_Y	63_L	0.587	1.00
76_S	79_L	0.586	1.00
126_Q	158_L	0.583	1.00
96_G	99_V	0.582	1.00
146_A	176_T	0.578	1.00
36_F	51_A	0.577	1.00
33_A	58_P	0.574	1.00
22_L	67_L	0.573	1.00
211_L	225_L	0.570	1.00
18_A	69_T	0.569	1.00
63_L	90_V	0.569	1.00
100_L	128_G	0.563	1.00
37_T	63_L	0.563	1.00
50_R	75_L	0.561	1.00
58_P	127_L	0.559	1.00
217_M	220_D	0.558	1.00
136_N	205_E	0.557	1.00
105_H	135_K	0.557	1.00
9_V	84_P	0.556	1.00
118_I	141_H	0.554	1.00
31_F	123_S	0.552	1.00
26_A	63_L	0.550	1.00
217_M	221_T	0.550	1.00
138_V	207_S	0.549	1.00
122_L	155_M	0.547	1.00
160_K	163_E	0.547	1.00
18_A	144_R	0.546	1.00
125_L	128_G	0.543	1.00
173_S	220_D	0.540	1.00
172_V	176_T	0.539	1.00
183_G	186_P	0.531	1.00
184_R	187_A	0.529	1.00
56_N	60_N	0.528	1.00
67_L	76_S	0.524	1.00
32_T	102_E	0.523	1.00
114_T	142_A	0.520	1.00
105_H	133_R	0.520	1.00
158_L	162_Y	0.518	1.00
64_E	154_L	0.517	1.00
156_Q	168_I	0.516	1.00
201_P	205_E	0.515	1.00
106_C	208_H	0.514	1.00
163_E	166_L	0.513	1.00
140_V	189_I	0.512	1.00
42_E	45_G	0.512	1.00
189_I	198_I	0.510	1.00
24_V	90_V	0.504	1.00
20_F	80_A	0.503	1.00
136_N	165_K	0.503	1.00
66_Y	116_T	0.502	1.00
100_L	105_H	0.502	1.00
109_L	210_M	0.501	1.00
151_Y	155_M	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1fdrA	1	0.9798	100	0.22	Contact Map
1gvhA	3	0.9637	100	0.228	Contact Map
1cqxA	2	0.9516	100	0.244	Contact Map
3jqqA	2	0.9395	100	0.249	Contact Map
2bgiA	1	0.9718	100	0.249	Contact Map
1krhA	1	0.9234	100	0.253	Contact Map
4b4dA	2	0.9637	100	0.256	Contact Map
2qdxA	1	0.9798	100	0.259	Contact Map
4fk8A	1	0.9556	100	0.259	Contact Map
4p6vF	1	0.9435	100	0.266	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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