GREMLIN Database
RFH - Putative peptide chain release factor homolog
UniProt: P28369 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11496
Length: 166 (162)
Sequences: 4146 (1582)
Seq/√Len: 124.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
131_Q134_N4.5071.00
129_Q133_E3.5851.00
133_E137_A3.1091.00
77_R90_D3.1051.00
130_Q134_N2.9251.00
72_R98_L2.8131.00
76_L105_K2.7821.00
73_Y95_A2.6711.00
71_I95_A2.6081.00
85_H89_T2.3391.00
81_P85_H2.2661.00
113_H117_R2.2491.00
45_R49_G2.1991.00
95_A120_R2.1351.00
78_S85_H2.1111.00
71_I120_R2.0811.00
128_E132_Q2.0661.00
45_R51_K2.0341.00
134_N138_L2.0221.00
132_Q135_S1.9251.00
85_H110_R1.9001.00
108_S118_L1.8981.00
81_P110_R1.8971.00
85_H91_S1.8641.00
132_Q136_A1.8551.00
72_R96_T1.8391.00
109_E114_A1.8251.00
131_Q135_S1.8201.00
19_S62_A1.7851.00
74_E96_T1.7841.00
85_H88_K1.7771.00
75_T90_D1.7581.00
13_L16_A1.7531.00
138_L141_Q1.7471.00
97_H100_S1.7191.00
41_P48_H1.6961.00
5_E13_L1.6571.00
75_T93_V1.5811.00
96_T103_S1.5681.00
92_A107_Q1.5561.00
122_L126_K1.5431.00
97_H101_G1.5301.00
136_A139_K1.4731.00
118_L121_L1.4251.00
45_R48_H1.4211.00
73_Y116_K1.3861.00
109_E118_L1.3781.00
39_I157_T1.3491.00
147_H153_N1.3411.00
117_R121_L1.3401.00
74_E94_R1.3321.00
113_H116_K1.3221.00
76_L94_R1.3101.00
68_S71_I1.2911.00
102_I130_Q1.2781.00
161_M164_I1.2651.00
85_H113_H1.2441.00
5_E16_A1.2400.99
33_C58_G1.2360.99
108_S122_L1.2240.99
19_S121_L1.2220.99
124_A128_E1.2210.99
54_F148_Q1.2010.99
20_L29_S1.1890.99
143_R146_H1.1790.99
85_H90_D1.1440.99
139_K143_R1.1080.99
133_E136_A1.1070.99
154_P163_F1.1070.99
66_E120_R1.0970.99
39_I159_T1.0820.99
78_S88_K1.0540.98
71_I98_L1.0400.98
135_S138_L1.0390.98
58_G143_R1.0370.98
76_L107_Q1.0310.98
3_E19_S1.0180.98
89_T111_S1.0150.98
140_S143_R1.0140.98
73_Y120_R0.9930.98
114_A121_L0.9710.97
75_T85_H0.9600.97
35_T56_G0.9550.97
114_A117_R0.9550.97
35_T145_F0.9490.97
106_V118_L0.9440.97
71_I99_A0.9390.97
104_V127_L0.9280.96
79_S107_Q0.9270.96
56_G149_I0.9210.96
130_Q133_E0.9140.96
70_A99_A0.9040.96
25_A29_S0.9030.96
134_N137_A0.8830.95
95_A116_K0.8820.95
41_P47_H0.8630.94
151_R160_G0.8530.94
129_Q132_Q0.8490.94
127_L130_Q0.8410.94
78_S92_A0.8390.94
4_T17_L0.8320.93
136_A140_S0.8210.93
140_S144_M0.8190.93
54_F150_E0.8160.93
105_K122_L0.8090.92
38_W160_G0.7990.92
33_C145_F0.7960.92
35_T54_F0.7900.91
77_R85_H0.7890.91
76_L103_S0.7870.91
63_D128_E0.7850.91
54_F153_N0.7850.91
3_E122_L0.7740.91
80_G87_N0.7730.91
28_L31_S0.7730.91
85_H111_S0.7600.90
9_Y154_P0.7580.90
22_G25_A0.7580.90
120_R124_A0.7570.90
86_V90_D0.7570.90
108_S114_A0.7500.89
68_S120_R0.7480.89
79_S86_V0.7480.89
35_T153_N0.7460.89
137_A140_S0.7380.88
37_Q52_N0.7350.88
36_I40_C0.7350.88
105_K108_S0.7340.88
41_P161_M0.7270.88
95_A104_V0.7250.88
58_G145_F0.7240.87
87_N91_S0.7160.87
38_W42_S0.7160.87
88_K93_V0.7110.86
58_G149_I0.7090.86
8_R11_D0.7080.86
79_S89_T0.7070.86
118_L122_L0.7000.86
95_A119_A0.6990.86
127_L131_Q0.6980.85
6_T15_S0.6860.84
54_F152_G0.6850.84
27_A30_E0.6850.84
106_V122_L0.6850.84
151_R157_T0.6830.84
116_K120_R0.6650.83
32_W40_C0.6630.82
78_S107_Q0.6620.82
67_Q70_A0.6610.82
32_W36_I0.6600.82
91_S113_H0.6560.82
63_D125_W0.6560.82
35_T146_H0.6550.82
86_V107_Q0.6550.82
110_R121_L0.6520.81
39_I42_S0.6350.80
88_K114_A0.6320.79
40_C52_N0.6280.79
58_G126_K0.6260.79
142_R145_F0.6250.79
42_S50_R0.6230.78
4_T15_S0.6230.78
21_D25_A0.6200.78
77_R89_T0.6190.78
149_I153_N0.6160.78
93_V104_V0.6150.77
88_K91_S0.6130.77
75_T81_P0.6130.77
58_G146_H0.6110.77
13_L17_L0.6020.76
50_R54_F0.6020.76
16_A57_I0.6020.76
4_T7_G0.5990.76
4_T105_K0.5980.76
75_T88_K0.5910.75
11_D53_W0.5890.74
76_L92_A0.5870.74
121_L125_W0.5860.74
104_V122_L0.5840.74
73_Y93_V0.5830.74
154_P159_T0.5800.73
160_G164_I0.5780.73
63_D124_A0.5750.73
24_N28_L0.5710.72
128_E131_Q0.5710.72
69_D98_L0.5700.72
35_T55_L0.5690.72
75_T113_H0.5660.71
78_S81_P0.5640.71
88_K105_K0.5610.71
40_C53_W0.5610.71
40_C51_K0.5570.70
100_S127_L0.5550.70
64_E146_H0.5550.70
94_R103_S0.5540.70
104_V126_K0.5540.70
58_G142_R0.5540.70
42_S154_P0.5530.70
151_R154_P0.5520.70
56_G147_H0.5500.69
139_K142_R0.5490.69
74_E126_K0.5490.69
79_S87_N0.5470.69
32_W158_F0.5460.69
38_W55_L0.5460.69
98_L101_G0.5450.69
121_L124_A0.5430.68
114_A118_L0.5430.68
106_V109_E0.5390.68
22_G128_E0.5380.68
74_E103_S0.5380.68
79_S105_K0.5330.67
97_H127_L0.5320.67
32_W37_Q0.5300.67
150_E153_N0.5290.66
64_E135_S0.5280.66
44_Y48_H0.5280.66
41_P163_F0.5270.66
84_Q87_N0.5270.66
42_S54_F0.5260.66
24_N27_A0.5250.66
142_R146_H0.5240.66
43_P47_H0.5210.65
74_E98_L0.5210.65
30_E63_D0.5200.65
4_T43_P0.5160.64
66_E124_A0.5140.64
75_T108_S0.5140.64
38_W44_Y0.5140.64
48_H53_W0.5140.64
21_D62_A0.5110.64
74_E107_Q0.5100.64
31_S147_H0.5100.64
37_Q157_T0.5090.63
18_V54_F0.5080.63
41_P162_A0.5080.63
58_G147_H0.5080.63
41_P49_G0.5040.63
38_W54_F0.5030.63
109_E121_L0.5020.62
96_T105_K0.5020.62
38_W50_R0.5000.62
36_I52_N0.5000.62
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2ihr1 1 1 100 0.09 Contact Map
1zbtA 1 0.9217 100 0.09 Contact Map
3d5aX 1 1 100 0.094 Contact Map
2b3tB 1 0.9217 100 0.102 Contact Map
1rq0A 3 0.9157 100 0.103 Contact Map
1gqeA 1 1 100 0.11 Contact Map
2rsmA 1 0.6627 100 0.464 Contact Map
3j7yp 1 0.3494 100 0.533 Contact Map
2jvaA 1 0.506 99.9 0.608 Contact Map
1j26A 1 0.5 99.9 0.609 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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