RODZ - ECOLI - GREMLIN Contacts

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RODZ - Cytoskeleton protein RodZ

UniProt:	P27434	Sequences: MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTVRDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSLGKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDRKAQQEEITTMADQSSAELSSNSEQGQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANVDTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLFSGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPAQ	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG10015
Length:	337 (312)
Sequences:	1219 (1129)
Seq/√Len:	63.9

ECOLI - RODZ
BACSU - YMFM

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
259_F	280_Q	5.086	1.00
261_A	303_A	4.788	1.00
33_V	71_L	4.784	1.00
14_T	50_D	4.404	1.00
266_E	277_S	3.933	1.00
22_A	26_L	3.619	1.00
20_R	24_E	3.292	1.00
290_G	294_Y	3.149	1.00
30_Q	44_V	3.123	1.00
51_K	54_A	2.924	1.00
39_L	43_T	2.920	1.00
262_D	281_R	2.860	1.00
260_T	305_Q	2.856	1.00
37_L	293_P	2.707	1.00
258_N	285_N	2.649	1.00
45_R	49_E	2.620	1.00
264_W	299_G	2.582	1.00
33_V	68_Y	2.543	1.00
43_T	64_Y	2.534	1.00
268_T	274_K	2.525	1.00
266_E	297_K	2.503	1.00
98_Q	101_S	2.426	1.00
47_I	65_I	2.403	1.00
22_A	72_V	2.362	1.00
276_F	286_L	2.336	1.00
300_A	303_A	2.306	1.00
297_K	326_R	2.290	1.00
22_A	25_Q	2.262	1.00
62_R	84_E	2.205	1.00
263_C	298_I	2.192	1.00
46_D	51_K	2.088	1.00
39_L	64_Y	2.087	1.00
46_D	55_D	2.041	1.00
38_C	67_S	1.980	1.00
259_F	304_V	1.947	1.00
263_C	304_V	1.912	1.00
30_Q	41_V	1.855	1.00
253_N	309_Q	1.849	1.00
40_K	67_S	1.809	1.00
278_G	284_G	1.806	1.00
57_A	64_Y	1.803	1.00
60_F	64_Y	1.794	1.00
262_D	279_M	1.791	1.00
205_P	239_P	1.788	1.00
63_G	66_R	1.748	1.00
75_P	78_E	1.691	0.99
298_I	304_V	1.683	0.99
258_N	305_Q	1.677	0.99
281_R	284_G	1.666	0.99
15_T	52_A	1.665	0.99
40_K	43_T	1.665	0.99
256_V	287_N	1.656	0.99
38_C	64_Y	1.619	0.99
188_A	195_A	1.619	0.99
217_N	223_T	1.617	0.99
47_I	72_V	1.600	0.99
196_V	199_P	1.590	0.99
257_M	267_V	1.584	0.99
18_R	79_L	1.562	0.99
37_L	71_L	1.533	0.99
203_V	208_N	1.526	0.99
213_P	218_V	1.512	0.99
42_S	64_Y	1.487	0.98
189_T	192_Q	1.485	0.98
301_P	323_Q	1.484	0.98
38_C	61_L	1.481	0.98
44_V	71_L	1.479	0.98
306_I	315_L	1.455	0.98
30_Q	259_F	1.455	0.98
268_T	297_K	1.421	0.98
210_V	215_Q	1.401	0.98
290_G	293_P	1.396	0.98
22_A	74_I	1.390	0.97
44_V	266_E	1.365	0.97
280_Q	284_G	1.359	0.97
34_A	47_I	1.350	0.97
266_E	274_K	1.344	0.97
127_L	131_W	1.340	0.97
217_N	220_T	1.336	0.97
172_T	177_T	1.328	0.97
97_M	100_F	1.325	0.97
230_T	234_G	1.316	0.96
255_L	329_L	1.308	0.96
41_V	45_R	1.293	0.96
259_F	266_E	1.286	0.96
21_N	24_E	1.280	0.96
274_K	277_S	1.272	0.96
17_A	20_R	1.245	0.95
74_I	78_E	1.240	0.95
28_L	71_L	1.232	0.95
127_L	130_A	1.231	0.95
15_T	86_Q	1.226	0.95
28_L	33_V	1.223	0.95
64_Y	259_F	1.219	0.95
190_N	196_V	1.218	0.95
208_N	213_P	1.212	0.94
102_L	298_I	1.210	0.94
39_L	325_A	1.203	0.94
21_N	25_Q	1.196	0.94
59_T	313_V	1.195	0.94
29_S	32_A	1.193	0.94
19_L	23_R	1.190	0.94
68_Y	71_L	1.178	0.93
263_C	322_N	1.177	0.93
131_W	275_L	1.173	0.93
132_W	136_R	1.170	0.93
158_S	163_S	1.167	0.93
191_T	211_V	1.157	0.93
314_D	318_F	1.157	0.93
62_R	80_L	1.151	0.93
186_T	193_T	1.149	0.93
50_D	61_L	1.138	0.92
38_C	66_R	1.136	0.92
323_Q	327_L	1.134	0.92
117_T	132_W	1.129	0.92
153_E	156_S	1.125	0.92
14_T	44_V	1.115	0.91
298_I	325_A	1.111	0.91
62_R	88_P	1.110	0.91
31_Q	44_V	1.108	0.91
255_L	308_Y	1.107	0.91
314_D	325_A	1.103	0.91
20_R	49_E	1.101	0.91
267_V	272_G	1.083	0.90
155_S	161_G	1.072	0.90
167_N	236_A	1.072	0.90
47_I	78_E	1.063	0.89
168_T	205_P	1.060	0.89
156_S	170_T	1.059	0.89
15_T	79_L	1.058	0.89
201_P	211_V	1.054	0.89
29_S	49_E	1.049	0.89
261_A	300_A	1.047	0.89
33_V	44_V	1.034	0.88
192_Q	207_Q	1.032	0.88
63_G	71_L	1.026	0.88
61_L	83_L	1.021	0.87
38_C	280_Q	1.019	0.87
65_I	80_L	1.018	0.87
57_A	61_L	1.010	0.87
295_K	328_T	1.006	0.87
139_Q	142_E	1.002	0.86
301_P	317_R	1.000	0.86
158_S	161_G	0.996	0.86
17_A	21_N	0.990	0.86
308_Y	330_N	0.988	0.86
263_C	288_L	0.987	0.86
234_G	237_P	0.985	0.85
186_T	204_D	0.982	0.85
302_A	317_R	0.979	0.85
50_D	294_Y	0.978	0.85
206_Q	214_S	0.977	0.85
222_A	227_T	0.975	0.85
44_V	60_F	0.971	0.85
160_Q	165_P	0.970	0.85
256_V	285_N	0.963	0.84
32_A	41_V	0.959	0.84
205_P	209_A	0.953	0.84
269_D	293_P	0.953	0.84
278_G	286_L	0.952	0.83
259_F	314_D	0.943	0.83
261_A	282_K	0.937	0.82
261_A	304_V	0.936	0.82
36_R	71_L	0.932	0.82
198_A	227_T	0.929	0.82
172_T	237_P	0.927	0.82
114_M	117_T	0.925	0.82
191_T	196_V	0.923	0.82
212_S	221_A	0.922	0.81
206_Q	209_A	0.919	0.81
40_K	60_F	0.918	0.81
16_G	23_R	0.916	0.81
63_G	68_Y	0.910	0.81
63_G	67_S	0.910	0.81
55_D	61_L	0.907	0.80
269_D	290_G	0.907	0.80
175_A	227_T	0.906	0.80
172_T	176_T	0.904	0.80
257_M	304_V	0.895	0.79
161_G	164_V	0.894	0.79
187_T	224_P	0.893	0.79
203_V	209_A	0.893	0.79
215_Q	250_A	0.890	0.79
254_A	309_Q	0.886	0.79
276_F	284_G	0.884	0.79
160_Q	169_S	0.883	0.79
162_Q	166_L	0.882	0.78
167_N	237_P	0.881	0.78
186_T	219_D	0.880	0.78
216_A	223_T	0.879	0.78
70_R	76_E	0.878	0.78
33_V	72_V	0.875	0.78
150_S	154_L	0.875	0.78
300_A	324_V	0.872	0.78
118_W	124_V	0.871	0.78
197_T	220_T	0.870	0.78
223_T	236_A	0.868	0.77
31_Q	35_E	0.868	0.77
177_T	205_P	0.866	0.77
120_V	126_G	0.865	0.77
200_A	205_P	0.865	0.77
205_P	208_N	0.863	0.77
207_Q	239_P	0.860	0.77
206_Q	211_V	0.856	0.76
268_T	281_R	0.855	0.76
128_S	135_D	0.853	0.76
57_A	277_S	0.852	0.76
114_M	118_W	0.851	0.76
168_T	172_T	0.851	0.76
280_Q	286_L	0.850	0.76
205_P	211_V	0.847	0.76
171_T	174_P	0.846	0.76
65_I	83_L	0.845	0.75
111_G	130_A	0.843	0.75
18_R	22_A	0.839	0.75
185_D	189_T	0.837	0.75
172_T	248_P	0.837	0.75
167_N	171_T	0.836	0.75
65_I	79_L	0.835	0.75
43_T	46_D	0.833	0.74
230_T	235_A	0.831	0.74
215_Q	233_D	0.831	0.74
226_P	229_A	0.826	0.74
254_A	289_T	0.821	0.73
252_P	289_T	0.818	0.73
30_Q	34_A	0.813	0.73
50_D	67_S	0.813	0.73
210_V	229_A	0.812	0.72
47_I	294_Y	0.811	0.72
275_L	279_M	0.810	0.72
196_V	204_D	0.809	0.72
22_A	62_R	0.804	0.72
22_A	31_Q	0.799	0.71
191_T	237_P	0.796	0.71
208_N	216_A	0.794	0.71
100_F	112_W	0.793	0.71
256_V	279_M	0.793	0.71
152_A	170_T	0.793	0.71
210_V	216_A	0.793	0.71
40_K	64_Y	0.791	0.71
114_M	134_Q	0.789	0.70
117_T	126_G	0.788	0.70
254_A	287_N	0.784	0.70
262_D	282_K	0.783	0.70
264_W	267_V	0.779	0.69
39_L	60_F	0.777	0.69
204_D	228_A	0.777	0.69
221_A	225_A	0.774	0.69
158_S	165_P	0.770	0.68
297_K	324_V	0.769	0.68
211_V	223_T	0.766	0.68
197_T	209_A	0.761	0.68
197_T	200_A	0.761	0.68
156_S	159_E	0.758	0.67
193_T	222_A	0.758	0.67
223_T	233_D	0.755	0.67
173_D	185_D	0.753	0.67
98_Q	103_G	0.752	0.67
302_A	323_Q	0.748	0.66
26_L	73_H	0.748	0.66
70_R	324_V	0.747	0.66
109_R	124_V	0.739	0.65
65_I	297_K	0.738	0.65
184_V	194_P	0.737	0.65
271_T	292_A	0.735	0.65
116_F	121_L	0.735	0.65
118_W	130_A	0.733	0.65
175_A	187_T	0.731	0.64
208_N	215_Q	0.730	0.64
227_T	230_T	0.726	0.64
293_P	326_R	0.721	0.63
58_S	83_L	0.719	0.63
307_Q	311_K	0.717	0.63
78_E	81_P	0.716	0.63
23_R	48_E	0.713	0.62
174_P	177_T	0.712	0.62
170_T	174_P	0.711	0.62
157_N	165_P	0.711	0.62
97_M	101_S	0.707	0.62
276_F	298_I	0.706	0.62
263_C	278_G	0.705	0.62
42_S	57_A	0.704	0.61
67_S	124_V	0.704	0.61
113_L	133_W	0.703	0.61
55_D	301_P	0.701	0.61
153_E	212_S	0.700	0.61
162_Q	169_S	0.696	0.61
203_V	213_P	0.694	0.60
165_P	171_T	0.692	0.60
265_L	276_F	0.692	0.60
34_A	63_G	0.689	0.60
142_E	146_M	0.689	0.60
218_V	226_P	0.686	0.60
42_S	88_P	0.682	0.59
222_A	232_P	0.678	0.59
65_I	86_Q	0.677	0.58
326_R	329_L	0.676	0.58
174_P	196_V	0.676	0.58
39_L	259_F	0.673	0.58
60_F	70_R	0.673	0.58
47_I	79_L	0.672	0.58
121_L	134_Q	0.669	0.58
171_T	175_A	0.669	0.58
15_T	84_E	0.666	0.57
219_D	239_P	0.664	0.57
173_D	215_Q	0.664	0.57
194_P	199_P	0.661	0.57
201_P	221_A	0.660	0.57
107_K	112_W	0.660	0.57
117_T	280_Q	0.660	0.57
214_S	220_T	0.659	0.56
46_D	108_K	0.659	0.56
260_T	299_G	0.659	0.56
207_Q	213_P	0.652	0.56
185_D	193_T	0.651	0.56
119_L	122_F	0.651	0.56
59_T	63_G	0.645	0.55
166_L	169_S	0.640	0.54
44_V	57_A	0.639	0.54
184_V	214_S	0.638	0.54
197_T	239_P	0.637	0.54
110_D	113_L	0.636	0.54
323_Q	326_R	0.634	0.54
216_A	226_P	0.633	0.53
44_V	56_L	0.633	0.53
72_V	135_D	0.633	0.53
64_Y	68_Y	0.632	0.53
40_K	55_D	0.628	0.53
62_R	255_L	0.628	0.53
203_V	210_V	0.625	0.53
189_T	194_P	0.625	0.53
41_V	294_Y	0.625	0.53
185_D	232_P	0.621	0.52
206_Q	217_N	0.620	0.52
227_T	239_P	0.619	0.52
277_S	297_K	0.618	0.52
255_L	298_I	0.618	0.52
110_D	114_M	0.616	0.52
197_T	205_P	0.616	0.52
16_G	19_L	0.616	0.52
140_Q	143_I	0.614	0.51
167_N	221_A	0.612	0.51
210_V	243_A	0.612	0.51
192_Q	230_T	0.611	0.51
201_P	206_Q	0.610	0.51
202_A	227_T	0.607	0.50
60_F	259_F	0.607	0.50
213_P	230_T	0.607	0.50
214_S	241_D	0.607	0.50
317_R	321_T	0.605	0.50
259_F	284_G	0.603	0.50
161_G	171_T	0.603	0.50
65_I	68_Y	0.603	0.50
189_T	193_T	0.603	0.50
56_L	61_L	0.602	0.50
296_L	327_L	0.600	0.50
157_N	162_Q	0.599	0.50
263_C	286_L	0.599	0.50
262_D	312_P	0.598	0.49
186_T	190_N	0.598	0.49
15_T	32_A	0.598	0.49
31_Q	41_V	0.597	0.49
220_T	234_G	0.592	0.49
37_L	288_L	0.592	0.49
176_T	188_A	0.591	0.49
195_A	226_P	0.586	0.48
166_L	224_P	0.584	0.48
114_M	127_L	0.583	0.48
191_T	202_A	0.583	0.48
273_K	277_S	0.582	0.48
212_S	228_A	0.582	0.48
30_Q	45_R	0.581	0.47
109_R	112_W	0.579	0.47
34_A	37_L	0.579	0.47
268_T	272_G	0.576	0.47
220_T	227_T	0.575	0.47
155_S	210_V	0.572	0.46
212_S	217_N	0.572	0.46
192_Q	238_L	0.568	0.46
161_G	166_L	0.568	0.46
206_Q	210_V	0.568	0.46
278_G	290_G	0.568	0.46
212_S	230_T	0.567	0.46
214_S	222_A	0.567	0.46
35_E	41_V	0.566	0.46
100_F	110_D	0.566	0.46
33_V	93_K	0.566	0.46
199_P	211_V	0.565	0.46
111_G	115_T	0.565	0.46
143_I	151_S	0.564	0.45
225_A	246_T	0.563	0.45
215_Q	241_D	0.562	0.45
22_A	303_A	0.562	0.45
189_T	211_V	0.561	0.45
184_V	213_P	0.560	0.45
62_R	304_V	0.560	0.45
291_Q	311_K	0.560	0.45
15_T	64_Y	0.559	0.45
240_T	245_V	0.558	0.45
226_P	231_T	0.558	0.45
14_T	47_I	0.557	0.45
268_T	310_G	0.555	0.44
138_A	142_E	0.554	0.44
66_R	329_L	0.551	0.44
57_A	301_P	0.551	0.44
171_T	209_A	0.550	0.44
121_L	129_G	0.548	0.44
66_R	80_L	0.547	0.44
158_S	164_V	0.547	0.44
269_D	288_L	0.547	0.44
228_A	231_T	0.546	0.43
297_K	325_A	0.546	0.43
285_N	305_Q	0.544	0.43
171_T	176_T	0.543	0.43
72_V	83_L	0.542	0.43
221_A	226_P	0.542	0.43
196_V	227_T	0.541	0.43
52_A	82_G	0.540	0.43
114_M	122_F	0.538	0.43
158_S	212_S	0.537	0.42
51_K	55_D	0.537	0.42
175_A	188_A	0.537	0.42
186_T	237_P	0.537	0.42
15_T	80_L	0.537	0.42
144_T	296_L	0.537	0.42
188_A	218_V	0.537	0.42
80_L	259_F	0.536	0.42
43_T	327_L	0.536	0.42
219_D	246_T	0.535	0.42
26_L	74_I	0.535	0.42
267_V	286_L	0.535	0.42
16_G	47_I	0.531	0.42
40_K	324_V	0.530	0.42
222_A	237_P	0.529	0.42
173_D	241_D	0.526	0.41
163_S	168_T	0.525	0.41
231_T	236_A	0.525	0.41
195_A	216_A	0.524	0.41
40_K	59_T	0.524	0.41
203_V	227_T	0.523	0.41
266_E	322_N	0.522	0.41
25_Q	58_S	0.519	0.40
187_T	192_Q	0.518	0.40
222_A	226_P	0.518	0.40
85_K	167_N	0.518	0.40
246_T	249_V	0.517	0.40
224_P	230_T	0.517	0.40
227_T	231_T	0.516	0.40
142_E	147_A	0.514	0.40
307_Q	312_P	0.513	0.40
270_A	293_P	0.513	0.40
194_P	223_T	0.513	0.40
33_V	63_G	0.513	0.40
120_V	131_W	0.511	0.39
190_N	198_A	0.509	0.39
159_E	164_V	0.508	0.39
37_L	262_D	0.508	0.39
264_W	297_K	0.506	0.39
79_L	82_G	0.506	0.39
79_L	271_T	0.505	0.39
25_Q	261_A	0.504	0.39
208_N	211_V	0.504	0.39
32_A	299_G	0.503	0.39
114_M	130_A	0.502	0.39
197_T	224_P	0.502	0.39
69_A	73_H	0.501	0.38
121_L	125_I	0.501	0.38
18_R	40_K	0.500	0.38

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3fymA	1	0.2433	99.7	0.747	Contact Map
4gqmA	2	0.2849	99.2	0.808	Contact Map
2wusR	1	0.2582	99.2	0.809	Contact Map
3eusA	2	0.2404	98.3	0.864	Contact Map
3kz3A	2	0.2344	98.2	0.864	Contact Map
1y7yA	2	0.2047	98.2	0.865	Contact Map
2ef8A	2	0.2493	98.2	0.865	Contact Map
1lmb3	2	0.2641	98.2	0.866	Contact Map
2ofyA	2	0.2374	98.2	0.866	Contact Map
3f6wA	2	0.2404	98.2	0.867	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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