GREMLIN Database
EMRA - Multidrug export protein EmrA
UniProt: P27303 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11354
Length: 390 (303)
Sequences: 19728 (15466)
Seq/√Len: 888.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
74_K91_T3.2781.00
84_K223_T3.1071.00
88_V217_R3.0311.00
290_V324_E2.8601.00
91_T217_R2.5671.00
271_P286_T2.4301.00
164_R168_T2.4001.00
287_G323_I2.3961.00
140_Q144_N2.3951.00
76_W91_T2.3871.00
105_K210_W2.3521.00
273_T284_K2.3411.00
146_R155_I2.2931.00
146_R163_A2.2741.00
167_V171_Q2.2351.00
147_V164_R2.2071.00
84_K87_D2.1891.00
94_P98_R2.1831.00
67_Q215_R2.1151.00
88_V219_I2.0981.00
82_F225_Y2.0851.00
85_E223_T2.0201.00
147_V151_N2.0171.00
101_F206_V1.9851.00
140_Q171_Q1.9841.00
255_D320_P1.9801.00
150_G157_R1.9611.00
273_T286_T1.9501.00
76_W88_V1.9451.00
138_K142_D1.9061.00
157_R161_Q1.8811.00
140_Q167_V1.8771.00
227_S247_V1.8571.00
253_W322_R1.8291.00
136_L167_V1.8191.00
288_K326_D1.8031.00
143_Y167_V1.8001.00
143_Y160_L1.7811.00
38_I42_W1.7511.00
40_I44_L1.7411.00
108_L206_V1.7371.00
159_E162_H1.7131.00
256_A343_V1.7121.00
143_Y147_V1.7081.00
101_F210_W1.7021.00
288_K324_E1.6931.00
133_K174_L1.6921.00
272_V323_I1.6781.00
102_E105_K1.6781.00
98_R102_E1.6651.00
150_G160_L1.6551.00
136_L140_Q1.6341.00
271_P288_K1.6241.00
101_F105_K1.6211.00
78_D81_D1.6141.00
57_V254_V1.6101.00
136_L171_Q1.5981.00
37_A41_Y1.5951.00
291_G322_R1.5851.00
137_A141_S1.5841.00
98_R213_L1.5731.00
103_K205_E1.5621.00
256_A323_I1.5271.00
160_L164_R1.5231.00
70_G92_L1.5021.00
40_I43_F1.5001.00
254_V341_T1.4991.00
200_Q203_A1.4951.00
143_Y164_R1.4841.00
123_K126_Q1.4821.00
105_K206_V1.4701.00
207_R210_W1.4651.00
39_G43_F1.4591.00
171_Q175_D1.4581.00
162_H166_A1.4501.00
130_E133_K1.4421.00
136_L174_L1.4401.00
72_V231_V1.4391.00
108_L203_A1.4281.00
133_K178_I1.4231.00
238_S241_T1.4231.00
142_D162_H1.4161.00
100_A103_K1.4121.00
82_F249_A1.4051.00
296_T319_L1.4011.00
72_V90_V1.4001.00
273_T346_N1.3801.00
73_T93_D1.3751.00
133_K137_A1.3751.00
168_T171_Q1.3741.00
206_V210_W1.3691.00
90_V218_I1.3601.00
251_N324_E1.3561.00
208_N212_A1.3541.00
166_A169_S1.3501.00
220_S246_V1.3441.00
73_T91_T1.3281.00
135_A169_S1.3231.00
129_I174_L1.3171.00
74_K233_P1.3121.00
100_A208_N1.2981.00
88_V91_T1.2971.00
147_V160_L1.2881.00
139_A170_A1.2811.00
145_R161_Q1.2761.00
203_A207_R1.2741.00
103_K107_A1.2711.00
25_L29_L1.2691.00
276_T341_T1.2661.00
259_K262_Q1.2591.00
107_A110_S1.2401.00
77_A87_D1.2341.00
61_Q245_A1.2291.00
101_F213_L1.2161.00
275_T344_S1.2121.00
211_L215_R1.2051.00
175_D178_I1.2031.00
100_A212_A1.2021.00
144_N148_P1.2001.00
226_V244_M1.1831.00
255_D322_R1.1801.00
108_L199_V1.1781.00
158_E162_H1.1741.00
131_V173_Q1.1741.00
273_T344_S1.1691.00
63_Q245_A1.1691.00
89_L244_M1.1581.00
148_P152_A1.1561.00
137_A140_Q1.1481.00
94_P216_T1.1461.00
173_Q176_V1.1361.00
129_I133_K1.1361.00
210_W214_E1.1331.00
166_A170_A1.1281.00
148_P151_N1.1261.00
97_A216_T1.1231.00
144_N147_V1.1141.00
275_T284_K1.1041.00
105_K109_A1.1021.00
198_A201_Q1.1001.00
141_S161_Q1.0981.00
66_S218_I1.0971.00
257_N320_P1.0921.00
329_Q334_P1.0891.00
174_L178_I1.0861.00
63_Q242_P1.0821.00
169_S173_Q1.0711.00
126_Q181_Y1.0671.00
62_I222_M1.0631.00
228_R245_A1.0621.00
137_A168_T1.0621.00
129_I181_Y1.0601.00
69_S239_P1.0561.00
129_I178_I1.0541.00
81_D87_D1.0541.00
286_T326_D1.0521.00
142_D158_E1.0511.00
115_T119_M1.0381.00
107_A201_Q1.0311.00
258_F321_V1.0311.00
237_I243_L1.0261.00
161_Q164_R1.0251.00
75_V90_V1.0041.00
102_E207_R1.0021.00
126_Q130_E0.9981.00
79_N228_R0.9981.00
36_V40_I0.9971.00
60_N248_P0.9931.00
132_Q170_A0.9921.00
283_V334_P0.9861.00
229_R232_Q0.9831.00
193_L196_Q0.9801.00
274_I325_L0.9801.00
289_V321_V0.9781.00
224_G246_V0.9741.00
258_F266_M0.9721.00
135_A138_K0.9651.00
199_V203_A0.9641.00
248_P251_N0.9611.00
67_Q71_S0.9591.00
75_V233_P0.9581.00
128_N131_V0.9561.00
205_E208_N0.9561.00
293_D320_P0.9531.00
77_A244_M0.9441.00
39_G42_W0.9371.00
131_V135_A0.9341.00
119_M196_Q0.9331.00
90_V243_L0.9321.00
220_S223_T0.9321.00
164_R167_V0.9261.00
57_V252_M0.9191.00
196_Q200_Q0.9191.00
94_P217_R0.9181.00
82_F223_T0.9171.00
115_T196_Q0.9161.00
107_A205_E0.9151.00
71_S236_Q0.9141.00
138_K162_H0.9011.00
163_A166_A0.8951.00
266_M345_V0.8941.00
253_W290_V0.8931.00
24_L27_L0.8911.00
138_K165_D0.8881.00
267_R270_Q0.8861.00
189_L192_K0.8831.00
128_N176_V0.8821.00
350_R353_Q0.8821.00
50_E344_S0.8821.00
346_N349_N0.8811.00
90_V244_M0.8791.00
37_A40_I0.8781.00
201_Q205_E0.8751.00
95_T211_L0.8741.00
155_I159_E0.8711.00
43_F46_L0.8631.00
110_S198_A0.8631.00
271_P346_N0.8621.00
211_L214_E0.8591.00
179_Q182_N0.8561.00
106_T204_T0.8541.00
266_M289_V0.8541.00
142_D146_R0.8541.00
91_T94_P0.8521.00
57_V335_L0.8491.00
173_Q177_A0.8491.00
141_S144_N0.8441.00
69_S236_Q0.8391.00
257_N318_R0.8361.00
146_R159_E0.8321.00
132_Q177_A0.8321.00
52_T344_S0.8311.00
78_D229_R0.8291.00
69_S238_S0.8281.00
104_A202_A0.8251.00
230_A245_A0.8241.00
55_A341_T0.8221.00
122_S126_Q0.8191.00
100_A104_A0.8161.00
254_V274_I0.8151.00
34_I38_I0.8151.00
135_A173_Q0.8141.00
263_I319_L0.8061.00
42_W45_V0.8041.00
119_M192_K0.8021.00
132_Q135_A0.8011.00
276_T279_Y0.8001.00
99_Q204_T0.7991.00
104_A107_A0.7911.00
128_N132_Q0.7901.00
99_Q211_L0.7891.00
107_A111_S0.7891.00
258_F345_V0.7881.00
72_V237_I0.7841.00
52_T342_L0.7831.00
254_V343_V0.7821.00
274_I287_G0.7811.00
170_A173_Q0.7791.00
135_A166_A0.7781.00
150_G155_I0.7781.00
77_A226_V0.7761.00
73_T94_P0.7741.00
26_L30_L0.7731.00
253_W324_E0.7711.00
66_S237_I0.7681.00
198_A202_A0.7661.00
71_S93_D0.7661.00
42_W46_L0.7621.00
83_V89_L0.7611.00
135_A170_A0.7571.00
275_T281_D0.7571.00
41_Y44_L0.7521.00
101_F209_A0.7501.00
96_D212_A0.7491.00
104_A209_A0.7491.00
229_R244_M0.7441.00
289_V323_I0.7431.00
124_Q180_Q0.7401.00
275_T342_L0.7351.00
41_Y45_V0.7341.00
133_K136_L0.7341.00
209_A212_A0.7251.00
135_A139_A0.7231.00
204_T207_R0.7201.00
23_L26_L0.7181.00
157_R160_L0.7181.00
127_A130_E0.7141.00
73_T76_W0.7071.00
85_E221_P0.7051.00
146_R158_E0.7031.00
44_L47_R0.7021.00
124_Q128_N0.7001.00
153_N157_R0.6981.00
29_L33_I0.6961.00
103_K208_N0.6941.00
109_A200_Q0.6931.00
139_A166_A0.6931.00
26_L29_L0.6931.00
170_A174_L0.6921.00
97_A100_A0.6891.00
67_Q96_D0.6881.00
100_A209_A0.6861.00
178_I182_N0.6851.00
159_E163_A0.6831.00
138_K141_S0.6781.00
180_Q183_A0.6761.00
147_V150_G0.6761.00
60_N232_Q0.6751.00
274_I341_T0.6741.00
38_I43_F0.6741.00
122_S181_Y0.6731.00
96_D100_A0.6691.00
64_I221_P0.6661.00
66_S243_L0.6651.00
43_F47_R0.6651.00
256_A345_V0.6651.00
172_A175_D0.6641.00
297_G320_P0.6631.00
90_V229_R0.6631.00
98_R101_F0.6621.00
59_G251_N0.6621.00
64_I244_M0.6611.00
298_S317_Q0.6611.00
272_V287_G0.6601.00
327_Q330_L0.6591.00
222_M246_V0.6521.00
66_S69_S0.6511.00
279_Y285_Y0.6501.00
42_W47_R0.6481.00
212_A215_R0.6471.00
76_W87_D0.6411.00
225_Y249_A0.6401.00
94_P213_L0.6401.00
252_M337_I0.6391.00
111_S202_A0.6391.00
132_Q173_Q0.6391.00
268_I292_L0.6381.00
128_N180_Q0.6371.00
274_I343_V0.6351.00
79_N229_R0.6351.00
214_E219_I0.6341.00
24_L28_T0.6341.00
23_L27_L0.6331.00
77_A81_D0.6311.00
134_I138_K0.6311.00
33_I37_A0.6281.00
165_D169_S0.6271.00
260_E340_S0.6271.00
290_V322_R0.6251.00
30_L34_I0.6241.00
171_Q174_L0.6201.00
126_Q129_I0.6191.00
218_I243_L0.6171.00
191_T194_E0.6151.00
22_R25_L0.6151.00
104_A108_L0.6141.00
309_T316_V0.6141.00
107_A198_A0.6131.00
97_A209_A0.6101.00
142_D163_A0.6091.00
97_A101_F0.6091.00
203_A206_V0.6091.00
134_I137_A0.6061.00
36_V39_G0.6041.00
319_L339_L0.6041.00
242_P245_A0.6031.00
274_I323_I0.6021.00
256_A321_V0.6011.00
68_V212_A0.5991.00
115_T199_V0.5961.00
44_L48_H0.5951.00
105_K108_L0.5931.00
125_L128_N0.5911.00
155_I160_L0.5871.00
270_Q347_T0.5871.00
202_A205_E0.5811.00
279_Y283_V0.5801.00
66_S90_V0.5771.00
128_N173_Q0.5711.00
77_A229_R0.5711.00
79_N322_R0.5701.00
176_V180_Q0.5691.00
49_F348_T0.5681.00
65_M236_Q0.5671.00
129_I177_A0.5661.00
97_A212_A0.5621.00
150_G153_N0.5601.00
21_K25_L0.5601.00
208_N211_L0.5571.00
268_I290_V0.5571.00
38_I41_Y0.5561.00
63_Q235_A0.5551.00
145_R158_E0.5541.00
214_E217_R0.5541.00
247_V337_I0.5541.00
269_G288_K0.5531.00
102_E106_T0.5501.00
202_A206_V0.5501.00
139_A163_A0.5491.00
194_E198_A0.5491.00
99_Q102_E0.5481.00
195_D198_A0.5461.00
107_A202_A0.5461.00
83_V220_S0.5441.00
141_S145_R0.5421.00
59_G324_E0.5411.00
156_G159_E0.5401.00
279_Y339_L0.5391.00
157_R164_R0.5381.00
132_Q174_L0.5371.00
228_R242_P0.5361.00
278_I339_L0.5321.00
168_T172_A0.5311.00
230_A242_P0.5301.00
22_R26_L0.5301.00
25_L28_T0.5301.00
68_V215_R0.5291.00
53_D256_A0.5281.00
59_G253_W0.5281.00
96_D215_R0.5271.00
95_T214_E0.5271.00
323_I343_V0.5271.00
296_T317_Q0.5271.00
108_L202_A0.5261.00
142_D166_A0.5261.00
272_V345_V0.5241.00
73_T233_P0.5221.00
139_A142_D0.5221.00
146_R150_G0.5211.00
68_V97_A0.5201.00
125_L129_I0.5201.00
138_K166_A0.5201.00
39_G44_L0.5191.00
123_K127_A0.5181.00
276_T285_Y0.5181.00
254_V325_L0.5181.00
207_R211_L0.5181.00
86_G219_I0.5161.00
130_E134_I0.5161.00
106_T109_A0.5161.00
257_N299_A0.5151.00
197_P200_Q0.5131.00
75_V229_R0.5111.00
262_Q265_N0.5071.00
72_V75_V0.5071.00
180_Q184_N0.5071.00
125_L177_A0.5071.00
258_F343_V0.5051.00
272_V343_V0.5041.00
161_Q165_D0.5041.00
277_D342_L0.5031.00
115_T121_N0.5021.00
336_R339_L0.5011.00
128_N177_A0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4dk0A 5 0.7718 100 0.344 Contact Map
3fppA 3 0.6744 100 0.354 Contact Map
4tkoB 1 0.7179 100 0.37 Contact Map
3lnnA 6 0.7974 100 0.401 Contact Map
1vf7A 5 0.5769 100 0.423 Contact Map
2f1mA 2 0.5974 100 0.436 Contact Map
4l8jA 2 0.7231 100 0.436 Contact Map
3ne5B 3 0.6564 100 0.439 Contact Map
4kkuA 1 0.5615 100 0.494 Contact Map
2k32A 1 0.2949 99.8 0.704 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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