GREMLIN Database
RFAL - O-antigen ligase
UniProt: P27243 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11424
Length: 419 (363)
Sequences: 8770 (8066)
Seq/√Len: 423.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
302_S307_R5.3371.00
299_F345_G5.0971.00
348_K352_G4.7431.00
307_R311_S4.5361.00
161_R169_A3.8901.00
343_E347_L3.7251.00
345_G353_I3.3251.00
173_A217_T3.0301.00
344_A356_T2.9791.00
84_L355_S2.9231.00
170_T214_T2.8591.00
295_G342_I2.8391.00
180_G202_S2.7851.00
361_F365_Y2.7061.00
295_G337_L2.6421.00
296_L300_I2.6151.00
292_Y342_I2.6081.00
181_I224_I2.5951.00
37_F41_T2.5351.00
339_N342_I2.4341.00
340_E386_G2.3721.00
300_I303_P2.2411.00
337_L343_E2.2411.00
298_I307_R2.2151.00
307_R346_S2.1841.00
365_Y369_K2.1751.00
309_A348_K2.0581.00
295_G299_F2.0481.00
146_I164_F2.0321.00
351_M355_S2.0201.00
213_Q218_L1.9931.00
150_A172_A1.9891.00
299_F350_L1.9691.00
143_S179_I1.9651.00
351_M354_F1.9371.00
177_M381_G1.8981.00
170_T217_T1.8881.00
88_W91_A1.8861.00
295_G346_S1.8781.00
353_I357_L1.8661.00
33_M37_F1.8651.00
133_E137_Y1.8511.00
77_S358_F1.8461.00
341_I387_L1.8301.00
216_A388_S1.8251.00
176_T212_T1.8221.00
402_I406_V1.8131.00
166_V175_S1.7811.00
88_W92_F1.7661.00
168_T389_D1.7631.00
92_F98_P1.7631.00
341_I383_V1.7541.00
299_F303_P1.7121.00
143_S205_V1.7061.00
297_N300_I1.7061.00
173_A212_T1.6891.00
140_Y183_S1.6801.00
170_T338_H1.6751.00
301_K319_L1.6461.00
224_I377_I1.6431.00
305_S351_M1.6401.00
398_P402_I1.6211.00
180_G221_F1.6131.00
220_L224_I1.6091.00
336_H389_D1.6051.00
291_M338_H1.6021.00
172_A176_T1.5811.00
385_I389_D1.5711.00
298_I346_S1.5541.00
176_T205_V1.5521.00
77_S355_S1.5411.00
177_M224_I1.5301.00
172_A212_T1.5211.00
134_S138_T1.5021.00
85_D88_W1.4871.00
299_F346_S1.4661.00
265_R268_E1.4641.00
291_M337_L1.4601.00
406_V410_V1.4461.00
84_L351_M1.4231.00
187_I202_S1.4151.00
76_L80_L1.4021.00
180_G205_V1.3631.00
298_I301_K1.3621.00
113_F117_S1.3591.00
192_K195_P1.3581.00
199_L203_C1.3551.00
216_A220_L1.3431.00
296_L299_F1.3371.00
374_G378_L1.3191.00
84_L88_W1.3091.00
138_T142_L1.2971.00
339_N387_L1.2941.00
375_L378_L1.2911.00
337_L346_S1.2911.00
220_L384_G1.2821.00
265_R269_A1.2771.00
293_E297_N1.2701.00
170_T389_D1.2631.00
137_Y141_S1.2581.00
168_T338_H1.2501.00
179_I205_V1.2501.00
129_K133_E1.2501.00
302_S305_S1.2471.00
112_I116_G1.2351.00
153_I164_F1.2331.00
310_E347_L1.2291.00
172_A208_V1.2191.00
92_F311_S1.2091.00
130_S134_S1.2081.00
295_G312_R1.2061.00
84_L91_A1.2051.00
120_V123_T1.2021.00
299_F351_M1.2001.00
228_A377_I1.1931.00
85_D348_K1.1931.00
119_I122_L1.1871.00
336_H390_V1.1871.00
146_I165_G1.1741.00
299_F353_I1.1691.00
311_S347_L1.1621.00
297_N301_K1.1621.00
80_L84_L1.1611.00
373_L377_I1.1581.00
344_A353_I1.1561.00
69_I72_L1.1541.00
181_I378_L1.1531.00
385_I390_V1.1391.00
336_H343_E1.1381.00
342_I345_G1.1371.00
343_E390_V1.1371.00
117_S121_F1.1311.00
312_R316_M1.1231.00
95_D98_P1.1221.00
109_T112_I1.1151.00
183_S201_N1.1131.00
285_L292_Y1.1021.00
180_G209_L1.1011.00
52_I56_L1.0981.00
161_R214_T1.0961.00
148_G152_Y1.0911.00
196_F199_L1.0871.00
196_F200_L1.0861.00
203_C207_Y1.0851.00
144_F201_N1.0841.00
207_Y211_L1.0841.00
358_F362_S1.0821.00
290_A293_E1.0811.00
399_I402_I1.0811.00
224_I381_G1.0801.00
341_I353_I1.0761.00
34_I37_F1.0761.00
187_I199_L1.0741.00
201_N205_V1.0731.00
284_S289_L1.0721.00
223_I384_G1.0721.00
37_F40_N1.0691.00
162_I208_V1.0681.00
295_G338_H1.0661.00
227_A377_I1.0621.00
367_A375_L1.0621.00
292_Y339_N1.0611.00
168_T171_G1.0601.00
385_I388_S1.0581.00
345_G348_K1.0581.00
147_A208_V1.0521.00
51_A55_L1.0501.00
209_L221_F1.0481.00
227_A231_A1.0391.00
354_F358_F1.0371.00
128_L131_K1.0371.00
294_I297_N1.0341.00
345_G352_G1.0341.00
287_A291_M1.0331.00
171_G336_H1.0311.00
286_G289_L1.0291.00
289_L293_E1.0251.00
312_R337_L1.0251.00
185_V189_Y1.0241.00
48_N52_I1.0211.00
190_T195_P1.0201.00
225_C229_L1.0181.00
186_A198_F1.0171.00
137_Y198_F1.0161.00
150_A162_I1.0111.00
170_T173_A1.0101.00
108_N112_I1.0031.00
182_V374_G1.0011.00
390_V393_W0.9971.00
49_I53_V0.9951.00
357_L361_F0.9911.00
150_A164_F0.9861.00
344_A348_K0.9811.00
298_I302_S0.9731.00
185_V374_G0.9731.00
239_K242_S0.9721.00
152_Y156_I0.9681.00
367_A408_L0.9621.00
147_A205_V0.9571.00
74_L77_S0.9561.00
215_R265_R0.9531.00
81_I85_D0.9521.00
177_M181_I0.9521.00
70_K73_I0.9491.00
341_I386_G0.9371.00
295_G345_G0.9361.00
209_L212_T0.9341.00
153_I162_I0.9301.00
338_H343_E0.9291.00
215_R285_L0.9261.00
81_I344_A0.9191.00
289_L292_Y0.9181.00
282_V286_G0.9141.00
312_R347_L0.9141.00
345_G351_M0.9121.00
214_T217_T0.9101.00
47_Y51_A0.9091.00
294_I298_I0.9041.00
84_L305_S0.9031.00
205_V208_V0.9021.00
137_Y186_A0.9011.00
285_L289_L0.9001.00
150_A153_I0.9001.00
178_L181_I0.8971.00
284_S287_A0.8951.00
75_P121_F0.8941.00
366_I369_K0.8931.00
388_S392_I0.8911.00
174_Y385_I0.8911.00
143_S208_V0.8881.00
79_F82_G0.8831.00
187_I198_F0.8801.00
263_Q267_N0.8771.00
116_G120_V0.8731.00
261_P264_N0.8721.00
362_S365_Y0.8711.00
301_K307_R0.8661.00
134_S137_Y0.8661.00
348_K351_M0.8661.00
50_T54_C0.8641.00
72_L75_P0.8621.00
378_L404_A0.8611.00
183_S205_V0.8591.00
191_K195_P0.8581.00
78_I85_D0.8551.00
206_L221_F0.8541.00
141_S145_L0.8531.00
57_S61_R0.8531.00
71_N75_P0.8501.00
164_F172_A0.8461.00
381_G385_I0.8441.00
131_K134_S0.8421.00
183_S202_S0.8381.00
61_R64_Q0.8371.00
170_T385_I0.8371.00
188_L228_A0.8281.00
147_A151_M0.8271.00
328_G331_E0.8271.00
368_Y371_R0.8251.00
147_A176_T0.8241.00
202_S206_L0.8241.00
283_T287_A0.8201.00
209_L217_T0.8201.00
389_D397_I0.8191.00
359_L363_L0.8181.00
220_L381_G0.8181.00
109_T113_F0.8111.00
259_N263_Q0.8101.00
80_L355_S0.8091.00
40_N44_I0.8081.00
227_A376_L0.8071.00
215_R284_S0.8071.00
340_E344_A0.8061.00
292_Y338_H0.8041.00
362_S366_I0.8031.00
184_G202_S0.8011.00
303_P307_R0.8001.00
153_I338_H0.8001.00
153_I163_S0.7961.00
292_Y337_L0.7911.00
341_I345_G0.7901.00
77_S80_L0.7901.00
79_F83_L0.7901.00
139_L179_I0.7881.00
147_A201_N0.7871.00
140_Y205_V0.7841.00
246_L250_I0.7841.00
144_F204_A0.7791.00
89_Y98_P0.7781.00
169_A173_A0.7781.00
182_V186_A0.7771.00
177_M217_T0.7761.00
220_L377_I0.7751.00
147_A204_A0.7741.00
308_S347_L0.7731.00
337_L342_I0.7731.00
338_H389_D0.7721.00
216_A389_D0.7711.00
69_I73_I0.7701.00
117_S120_V0.7681.00
121_F125_T0.7681.00
54_C58_L0.7671.00
288_R338_H0.7651.00
142_L175_S0.7641.00
376_L380_L0.7621.00
298_I312_R0.7611.00
41_T44_I0.7601.00
391_I397_I0.7601.00
299_F352_G0.7581.00
226_V230_I0.7541.00
394_A397_I0.7531.00
307_R347_L0.7491.00
341_I357_L0.7481.00
162_I211_L0.7471.00
342_I346_S0.7461.00
109_T120_V0.7451.00
89_Y92_F0.7431.00
130_S133_E0.7431.00
90_S93_K0.7411.00
112_I115_F0.7381.00
94_V97_S0.7361.00
216_A385_I0.7341.00
197_L201_N0.7311.00
385_I391_I0.7301.00
312_R346_S0.7301.00
283_T286_G0.7291.00
47_Y50_T0.7281.00
350_L354_F0.7251.00
72_L76_L0.7251.00
27_I31_F0.7251.00
88_W309_A0.7251.00
144_F148_G0.7241.00
88_W348_K0.7221.00
92_F95_D0.7201.00
136_L182_V0.7191.00
170_T216_A0.7171.00
246_L253_S0.7171.00
266_Y269_A0.7161.00
181_I185_V0.7131.00
33_M36_A0.7101.00
152_Y158_E0.7091.00
386_G391_I0.7081.00
302_S346_S0.7071.00
216_A295_G0.7071.00
328_G332_F0.7041.00
160_D338_H0.7041.00
38_V42_F0.7021.00
181_I381_G0.7011.00
83_L87_I0.7011.00
140_Y143_S0.6991.00
384_G388_S0.6981.00
250_I254_I0.6981.00
169_A214_T0.6961.00
75_P79_F0.6941.00
176_T208_V0.6931.00
40_N43_S0.6921.00
55_L58_L0.6911.00
109_T116_G0.6911.00
341_I356_T0.6911.00
170_T174_Y0.6891.00
77_S81_I0.6891.00
363_L401_I0.6881.00
315_S318_L0.6881.00
222_P226_V0.6871.00
291_M295_G0.6841.00
303_P345_G0.6821.00
337_L340_E0.6791.00
240_F243_S0.6781.00
336_H347_L0.6771.00
177_M221_F0.6761.00
254_I258_F0.6751.00
168_T340_E0.6751.00
398_P401_I0.6741.00
89_Y95_D0.6711.00
229_L233_Y0.6651.00
85_D89_Y0.6651.00
390_V394_A0.6651.00
171_G389_D0.6610.99
75_P78_I0.6610.99
253_S256_I0.6600.99
154_N162_I0.6590.99
395_R398_P0.6590.99
295_G298_I0.6540.99
243_S247_L0.6540.99
150_A208_V0.6540.99
264_N267_N0.6470.99
220_L385_I0.6470.99
355_S359_L0.6430.99
241_T244_I0.6400.99
114_I118_F0.6400.99
242_S245_V0.6390.99
30_F33_M0.6380.99
63_R66_N0.6370.99
233_Y236_S0.6360.99
215_R388_S0.6360.99
378_L381_G0.6340.99
345_G350_L0.6330.99
292_Y336_H0.6330.99
329_A332_F0.6310.99
368_Y376_L0.6290.99
151_M155_S0.6280.99
364_F380_L0.6270.99
136_L186_A0.6270.99
169_A212_T0.6260.99
296_L345_G0.6260.99
214_T288_R0.6220.99
216_A219_L0.6220.99
31_F35_I0.6210.99
168_T309_A0.6210.99
168_T214_T0.6210.99
386_G389_D0.6210.99
358_F361_F0.6210.99
248_I252_A0.6200.99
183_S198_F0.6190.99
253_S257_I0.6180.99
239_K243_S0.6170.99
291_M346_S0.6160.99
247_L251_L0.6120.99
130_S137_Y0.6120.99
336_H339_N0.6110.99
382_I391_I0.6100.99
337_L390_V0.6080.99
308_S312_R0.6060.99
65_E68_N0.6050.99
340_E343_E0.6040.99
296_L342_I0.6010.99
136_L140_Y0.6010.99
340_E391_I0.6010.99
291_M342_I0.6000.99
340_E356_T0.6000.99
85_D91_A0.5990.99
180_G183_S0.5980.99
125_T131_K0.5940.99
161_R213_Q0.5940.99
267_N270_L0.5920.99
73_I77_S0.5910.99
368_Y372_A0.5910.99
377_I381_G0.5910.99
112_I119_I0.5910.99
366_I370_K0.5900.99
26_Y30_F0.5890.99
298_I319_L0.5890.99
261_P265_R0.5890.99
260_K263_Q0.5880.99
163_S169_A0.5870.99
77_S359_L0.5850.99
340_E393_W0.5850.99
240_F244_I0.5850.99
248_I251_L0.5830.99
162_I169_A0.5830.99
355_S358_F0.5830.99
113_F116_G0.5820.99
73_I76_L0.5810.99
389_D395_R0.5810.99
77_S362_S0.5810.99
262_I266_Y0.5790.99
28_L33_M0.5760.99
81_I84_L0.5760.99
379_T401_I0.5730.99
115_F119_I0.5720.99
173_A177_M0.5720.99
294_I316_M0.5710.98
66_N69_I0.5710.98
190_T194_H0.5700.98
151_M204_A0.5680.98
364_F379_T0.5680.98
84_L348_K0.5670.98
168_T343_E0.5660.98
243_S246_L0.5660.98
343_E393_W0.5650.98
298_I316_M0.5650.98
161_R265_R0.5640.98
74_L362_S0.5630.98
95_D99_F0.5620.98
396_S402_I0.5620.98
200_L204_A0.5600.98
153_I158_E0.5600.98
399_I403_S0.5600.98
396_S400_I0.5600.98
396_S399_I0.5590.98
82_G85_D0.5570.98
337_L347_L0.5550.98
120_V126_S0.5540.98
112_I123_T0.5530.98
206_L210_A0.5520.98
230_I234_N0.5510.98
143_S147_A0.5490.98
198_F202_S0.5470.98
161_R212_T0.5470.98
307_R315_S0.5460.98
71_N79_F0.5450.98
58_L62_G0.5440.98
343_E346_S0.5420.98
37_F42_F0.5410.98
304_F351_M0.5400.98
53_V57_S0.5400.98
248_I255_V0.5400.98
343_E389_D0.5380.98
382_I390_V0.5360.98
242_S246_L0.5330.98
258_F261_P0.5320.98
173_A214_T0.5310.98
312_R343_E0.5300.98
47_Y54_C0.5300.98
65_E69_I0.5280.97
313_A343_E0.5250.97
245_V249_A0.5250.97
287_A292_Y0.5220.97
179_I182_V0.5220.97
110_A113_F0.5210.97
251_L255_V0.5210.97
25_T29_C0.5200.97
298_I315_S0.5190.97
218_L262_I0.5190.97
81_I88_W0.5190.97
118_F122_L0.5180.97
163_S168_T0.5180.97
51_A62_G0.5160.97
164_F175_S0.5150.97
151_M208_V0.5150.97
170_T390_V0.5130.97
109_T117_S0.5130.97
146_I150_A0.5110.97
252_A256_I0.5110.97
266_Y270_L0.5110.97
28_L32_S0.5100.97
129_K132_K0.5090.97
140_Y186_A0.5080.97
383_V386_G0.5080.97
382_I389_D0.5080.97
121_F124_L0.5070.97
169_A172_A0.5060.97
177_M385_I0.5050.97
382_I392_I0.5050.97
185_V188_L0.5050.97
262_I269_A0.5050.97
81_I348_K0.5050.97
282_V285_L0.5050.97
63_R68_N0.5040.97
183_S186_A0.5040.97
305_S309_A0.5030.97
176_T209_L0.5020.97
313_A317_N0.5010.97
389_D392_I0.5000.97
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3tx3A 2 0.1551 4.6 0.96 Contact Map
4ev6A 3 0.1575 4.6 0.96 Contact Map
3wxwA 1 0.2936 4.3 0.961 Contact Map
4i0uA 4 0.1527 3.9 0.962 Contact Map
3fymA 1 0 3.5 0.963 Contact Map
2rmfA 1 0.0573 2.9 0.964 Contact Map
2l27B 1 0.0573 2.7 0.964 Contact Map
1go9A 1 0.0573 2.5 0.965 Contact Map
4qncA 2 0.1862 2.3 0.966 Contact Map
2rm9A 1 0.0549 2.3 0.966 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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