GREMLIN Database
RFAJ - Lipopolysaccharide 1,2-glucosyltransferase
UniProt: P27129 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11353
Length: 338 (267)
Sequences: 2257 (1466)
Seq/√Len: 89.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
109_M181_S6.4701.00
32_Y45_S4.9661.00
124_D189_K4.3841.00
133_V183_V3.8051.00
61_Y89_Y3.7831.00
36_A71_F3.7231.00
52_N287_A3.3751.00
59_D87_T3.2061.00
127_L185_Y3.0251.00
181_S214_P2.8381.00
49_I60_F2.6751.00
154_V179_F2.6591.00
41_G284_Y2.6061.00
186_L191_W2.5711.00
179_F219_M2.5231.00
190_K193_D2.5051.00
117_Q197_T2.4771.00
188_L192_A2.4461.00
137_G261_L2.4441.00
109_M214_P2.4201.00
46_I60_F2.4121.00
45_S133_V2.3451.00
147_L153_A2.3301.00
29_N61_Y2.2761.00
136_K283_Y2.2761.00
233_E261_L2.2161.00
195_K198_E2.1281.00
147_L150_A2.0781.00
148_N228_L1.9941.00
149_G187_D1.9861.00
34_V42_V1.9701.00
190_K194_A1.9651.00
31_A126_L1.9191.00
135_C262_L1.9041.00
43_G293_W1.8911.00
44_V134_V1.8521.00
151_V194_A1.8451.00
51_L290_N1.8011.00
189_K193_D1.7991.00
194_A226_M1.7801.00
36_A68_N1.7631.00
234_Y261_L1.7561.00
31_A115_A1.7501.00
29_N59_D1.7321.00
180_N235_N1.7311.00
183_V262_L1.6731.00
123_L188_L1.6451.00
153_A228_L1.6321.00
140_S144_H1.6311.00
34_V131_A1.6231.00
151_V226_M1.6121.00
178_Y217_D1.6091.00
50_V84_L1.6021.00
53_N138_D1.5751.00
49_I139_I1.5701.00
203_I221_V1.5611.00
35_D108_A1.5601.00
29_N123_L1.5391.00
105_W214_P1.5331.00
187_D190_K1.5311.00
179_F227_T1.4981.00
129_L133_V1.4520.99
45_S49_I1.4390.99
119_L123_L1.4380.99
125_R187_D1.4320.99
56_I144_H1.4060.99
112_R128_Y1.3930.99
44_V288_L1.3710.99
286_I290_N1.3690.99
198_E202_S1.3640.99
43_G291_S1.3630.99
181_S215_D1.3610.99
64_A72_F1.3590.99
150_A228_L1.3540.99
50_V60_F1.3420.99
32_Y133_V1.3360.99
120_G192_A1.3150.99
150_A226_M1.3120.99
147_L187_D1.2950.99
202_S206_S1.2790.99
129_L183_V1.2680.99
141_Q261_L1.2550.98
32_Y42_V1.2510.98
94_D97_Q1.2420.98
64_A88_L1.2260.98
59_D85_R1.2100.98
133_V262_L1.2070.98
33_G112_R1.2020.98
137_G141_Q1.1960.98
165_A168_R1.1860.98
256_I260_T1.1710.98
72_F76_A1.1640.97
294_K297_S1.1470.97
285_K288_L1.1350.97
203_I207_K1.1210.97
28_L143_L1.1090.97
191_W196_L1.1080.97
237_I266_T1.1070.96
69_D73_Q1.1010.96
218_V222_L1.0980.96
130_D182_G1.0960.96
73_Q77_K1.0840.96
149_G190_K1.0820.96
151_V187_D1.0790.96
215_D220_N1.0790.96
29_N143_L1.0780.96
119_L126_L1.0610.96
282_K286_I1.0600.96
127_L143_L1.0590.95
72_F88_L1.0490.95
200_A222_L1.0440.95
54_R58_L1.0410.95
35_D110_Y1.0350.95
33_G63_I1.0290.95
137_G233_E1.0220.95
269_T273_H1.0190.94
53_N58_L1.0130.94
200_A218_V0.9950.94
30_V60_F0.9930.94
233_E259_S0.9880.93
129_L135_C0.9790.93
142_L230_L0.9780.93
260_T263_I0.9770.93
46_I50_V0.9740.93
136_K263_I0.9740.93
64_A67_Y0.9720.93
167_S171_D0.9690.93
47_T51_L0.9650.93
112_R215_D0.9630.93
49_I135_C0.9630.93
129_L262_L0.9580.92
125_R147_L0.9480.92
69_D72_F0.9350.91
116_F197_T0.9340.91
154_V227_T0.9340.91
154_V219_M0.9290.91
203_I218_V0.9250.91
68_N71_F0.9200.91
181_S219_M0.9170.91
71_F74_K0.9160.91
288_L291_S0.9160.91
190_K226_M0.9160.91
265_Y280_S0.9120.90
61_Y87_T0.9110.90
180_N216_Q0.9090.90
203_I222_L0.9080.90
62_I88_L0.9070.90
151_V190_K0.9020.90
252_Y256_I0.9000.90
113_L200_A0.8960.90
146_G150_A0.8870.89
96_L114_F0.8670.88
163_E167_S0.8650.88
214_P217_D0.8630.88
166_V171_D0.8630.88
157_D216_Q0.8610.88
219_M227_T0.8590.88
134_V265_Y0.8570.87
111_F284_Y0.8550.87
60_F135_C0.8550.87
147_L228_L0.8540.87
42_V46_I0.8500.87
29_N89_Y0.8470.87
151_V223_L0.8430.87
32_Y46_I0.8430.87
110_Y214_P0.8400.86
46_I62_I0.8380.86
109_M215_D0.8340.86
132_D264_H0.8320.86
128_Y220_N0.8300.86
179_F220_N0.8280.86
189_K192_A0.8220.85
36_A39_L0.8210.85
178_Y220_N0.8170.85
63_I115_A0.8150.85
70_G74_K0.8140.85
127_L139_I0.8090.84
56_I140_S0.8020.84
143_L159_E0.7960.83
40_D288_L0.7940.83
41_G288_L0.7940.83
293_W297_S0.7910.83
28_L127_L0.7830.82
56_I138_D0.7820.82
120_G188_L0.7800.82
28_L31_A0.7790.82
105_W109_M0.7740.82
119_L188_L0.7670.81
42_V131_A0.7610.80
39_L71_F0.7600.80
34_V64_A0.7590.80
147_L185_Y0.7580.80
202_S205_M0.7550.80
73_Q76_A0.7530.80
236_T280_S0.7500.79
154_V181_S0.7490.79
150_A153_A0.7480.79
146_G149_G0.7460.79
41_G270_K0.7380.78
65_D107_R0.7360.78
182_G264_H0.7330.78
249_H252_Y0.7320.78
187_D228_L0.7250.77
114_F201_L0.7240.77
43_G75_I0.7240.77
289_E295_D0.7200.77
38_Y104_V0.7180.76
48_S52_N0.7180.76
200_A223_L0.7170.76
150_A187_D0.7160.76
283_Y286_I0.7160.76
48_S287_A0.7160.76
117_Q198_E0.7150.76
167_S170_S0.7140.76
96_L99_L0.7100.76
29_N125_R0.7070.75
153_A187_D0.7060.75
134_V287_A0.7030.75
171_D175_L0.7030.75
134_V286_I0.7020.75
61_Y123_L0.7000.75
207_K210_V0.7000.75
49_I58_L0.6990.75
128_Y178_Y0.6980.74
152_A223_L0.6960.74
108_A111_F0.6930.74
142_L185_Y0.6930.74
50_V78_L0.6920.74
33_G111_F0.6910.74
48_S134_V0.6890.73
67_Y72_F0.6890.73
125_R149_G0.6860.73
272_W284_Y0.6860.73
67_Y71_F0.6860.73
280_S283_Y0.6840.73
46_I49_I0.6830.73
51_L82_N0.6830.73
91_I114_F0.6830.73
38_Y131_A0.6830.73
61_Y64_A0.6820.73
164_K172_P0.6790.72
253_K256_I0.6780.72
215_D235_N0.6770.72
43_G71_F0.6720.72
142_L234_Y0.6700.71
30_V49_I0.6690.71
134_V283_Y0.6690.71
191_W194_A0.6690.71
26_E124_D0.6690.71
128_Y184_V0.6680.71
237_I241_K0.6670.71
79_A82_N0.6660.71
203_I211_Y0.6660.71
40_D284_Y0.6660.71
143_L192_A0.6590.70
47_T82_N0.6580.70
166_V170_S0.6560.70
106_S214_P0.6550.70
32_Y135_C0.6550.70
145_L228_L0.6530.69
112_R219_M0.6520.69
157_D180_N0.6510.69
130_D219_M0.6510.69
80_E83_Q0.6510.69
216_Q220_N0.6480.69
41_G272_W0.6470.69
74_K77_K0.6460.69
114_F218_V0.6450.68
101_C107_R0.6420.68
77_K80_E0.6420.68
217_D220_N0.6390.68
152_A184_V0.6380.68
250_Q253_K0.6380.68
252_Y255_L0.6370.68
168_R171_D0.6370.68
199_K202_S0.6360.67
166_V169_L0.6340.67
57_N83_Q0.6320.67
196_L223_L0.6310.67
45_S135_C0.6310.67
101_C104_V0.6280.66
77_K225_G0.6280.66
154_V270_K0.6270.66
166_V173_E0.6260.66
98_C205_M0.6250.66
94_D117_Q0.6250.66
178_Y213_Y0.6230.66
115_A126_L0.6190.65
218_V267_G0.6150.65
105_W266_T0.6120.64
51_L287_A0.6110.64
264_H270_K0.6110.64
120_G123_L0.6090.64
134_V263_I0.6070.64
126_L192_A0.6050.64
100_P110_Y0.6040.63
116_F188_L0.6020.63
84_L234_Y0.6000.63
232_R256_I0.5980.63
60_F65_D0.5930.62
113_L215_D0.5930.62
93_T96_L0.5930.62
291_S295_D0.5910.62
44_V291_S0.5910.62
287_A290_N0.5910.62
132_D270_K0.5900.62
180_N183_V0.5900.62
30_V127_L0.5890.61
236_T284_Y0.5860.61
273_H276_A0.5850.61
104_V214_P0.5850.61
230_L234_Y0.5830.61
37_N66_V0.5820.61
137_G259_S0.5800.60
54_R139_I0.5800.60
152_A227_T0.5800.60
165_A169_L0.5780.60
170_S173_E0.5770.60
128_Y179_F0.5770.60
155_V183_V0.5770.60
156_K177_Q0.5750.60
113_L218_V0.5740.60
75_I79_A0.5730.59
289_E294_K0.5720.59
83_Q88_L0.5710.59
238_Y242_S0.5700.59
112_R184_V0.5700.59
60_F84_L0.5700.59
214_P223_L0.5680.59
38_Y106_S0.5660.58
128_Y181_S0.5660.58
94_D198_E0.5650.58
207_K211_Y0.5650.58
215_D219_M0.5640.58
234_Y288_L0.5630.58
128_Y215_D0.5610.58
164_K168_R0.5600.58
47_T290_N0.5590.58
213_Y217_D0.5590.58
33_G108_A0.5590.58
178_Y284_Y0.5570.57
28_L139_I0.5570.57
180_N266_T0.5540.57
169_L176_G0.5540.57
32_Y49_I0.5530.57
184_V219_M0.5520.57
211_Y217_D0.5500.56
125_R145_L0.5500.56
62_I269_T0.5490.56
293_W296_D0.5490.56
139_I262_L0.5480.56
47_T50_V0.5460.56
154_V229_F0.5450.56
200_A231_P0.5440.56
149_G202_S0.5430.55
191_W223_L0.5430.55
278_Y281_V0.5420.55
130_D264_H0.5400.55
79_A84_L0.5400.55
68_N277_I0.5400.55
33_G91_I0.5390.55
51_L149_G0.5380.55
233_E255_L0.5350.54
34_V38_Y0.5350.54
164_K167_S0.5320.54
199_K222_L0.5320.54
231_P255_L0.5320.54
95_K98_C0.5300.54
34_V41_G0.5290.53
169_L175_L0.5270.53
159_E266_T0.5270.53
34_V67_Y0.5260.53
153_A185_Y0.5250.53
132_D182_G0.5240.53
128_Y183_V0.5230.53
216_Q235_N0.5230.53
112_R181_S0.5210.52
257_T260_T0.5200.52
73_Q80_E0.5200.52
116_F191_W0.5200.52
128_Y180_N0.5200.52
125_R150_A0.5180.52
112_R178_Y0.5150.52
87_T95_K0.5150.52
124_D187_D0.5140.51
50_V79_A0.5140.51
105_W213_Y0.5140.51
91_I140_S0.5130.51
27_C59_D0.5130.51
284_Y288_L0.5130.51
34_V39_L0.5100.51
107_R183_V0.5100.51
39_L64_A0.5070.50
50_V82_N0.5070.50
124_D202_S0.5060.50
108_A112_R0.5060.50
81_Q85_R0.5060.50
177_Q229_F0.5050.50
54_R87_T0.5010.50
77_K125_R0.5000.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1g9rA 1 0.7899 100 0.331 Contact Map
3tztA 2 0.6982 100 0.337 Contact Map
1ll2A 2 0.7101 100 0.468 Contact Map
3u2uA 2 0.7101 100 0.468 Contact Map
4uegA 4 0.7101 100 0.47 Contact Map
2p6wA 1 0.5858 95.5 0.918 Contact Map
1xhbA 1 0.8166 93.9 0.925 Contact Map
4aylA 1 0.574 92.6 0.928 Contact Map
1o7qA 1 0.7219 90.2 0.932 Contact Map
3iohA 2 0.6953 87 0.936 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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