GREMLIN Database
RFAI - Lipopolysaccharide 1,3-galactosyltransferase
UniProt: P27128 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11352
Length: 339 (269)
Sequences: 2393 (1476)
Seq/√Len: 90.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
110_I184_S6.3171.00
33_Y46_S4.9851.00
125_P192_A4.5611.00
62_H90_Y3.9201.00
37_K72_D3.8011.00
134_I186_F3.7201.00
60_C88_K3.3781.00
184_S219_P3.2381.00
128_L188_L3.2281.00
53_Y289_A2.8711.00
155_M182_F2.7581.00
189_I194_W2.6641.00
50_I61_F2.6351.00
193_Q196_A2.5831.00
30_D62_H2.5131.00
46_S134_I2.5091.00
47_I61_F2.4741.00
191_T195_A2.4541.00
42_G286_F2.3981.00
52_K292_A2.3961.00
182_F224_L2.3681.00
110_I219_P2.3631.00
238_K262_I2.3381.00
148_F154_A2.3381.00
118_D200_S2.3051.00
198_Q201_A2.2511.00
138_G262_I2.1901.00
137_Q285_A2.1021.00
193_Q197_Q2.0501.00
37_K69_G1.9961.00
183_N240_N1.9731.00
150_D190_N1.9601.00
192_A196_A1.9391.00
184_S220_D1.9021.00
136_C263_F1.8991.00
30_D60_C1.8491.00
45_I135_I1.8491.00
149_P233_I1.8321.00
32_A116_I1.7881.00
148_F151_D1.7601.00
126_K190_N1.7391.00
197_Q231_K1.7151.00
35_T43_C1.7101.00
44_G295_W1.6991.00
36_D109_A1.6951.00
32_A127_V1.6941.00
106_W219_P1.6921.00
270_T274_H1.6581.00
46_S50_I1.6541.00
152_K197_Q1.6391.00
148_F190_N1.6331.00
181_Y222_D1.6331.00
206_M226_M1.6301.00
50_I140_I1.5621.00
201_A205_A1.5451.00
34_G113_R1.4971.00
124_A191_T1.4951.00
141_E145_N1.4901.00
182_F232_L1.4861.00
30_D124_A1.4811.00
288_E292_A1.4761.00
65_T73_R1.4761.00
239_Y262_I1.4721.00
130_L186_F1.4691.00
152_K231_K1.4600.99
138_G142_P1.4470.99
51_L85_T1.4260.99
55_E59_L1.4230.99
33_Y134_I1.4210.99
51_L61_F1.4190.99
44_G293_S1.4120.99
73_R77_D1.4080.99
35_T132_A1.3730.99
130_L134_I1.3340.99
65_T89_I1.3300.99
154_A233_I1.3300.99
60_C86_R1.3250.99
121_I195_A1.3170.99
190_N193_Q1.3120.99
57_S145_N1.3020.99
168_R171_S1.2840.99
54_N139_T1.2790.99
48_A52_K1.2660.99
120_F127_V1.2640.99
137_Q264_I1.2550.98
34_G64_F1.2440.98
277_A280_Y1.2080.98
113_R129_Y1.2010.98
33_Y43_C1.1940.98
120_F124_A1.1920.98
242_Q267_I1.1740.98
74_K78_A1.1680.97
296_K299_A1.1620.97
183_N221_Q1.1410.97
203_A223_V1.1270.97
219_P222_D1.1270.97
220_D225_N1.1260.97
57_S141_E1.1240.97
257_V261_T1.1240.97
70_D74_K1.1230.97
261_T264_I1.1220.97
70_D73_R1.1100.97
238_K260_D1.0910.96
186_F263_F1.0810.96
151_D233_I1.0730.96
47_I51_L1.0650.96
169_A172_L1.0630.96
155_M224_L1.0630.96
142_P262_I1.0610.96
206_M210_P1.0570.95
110_I220_D1.0520.95
99_S102_S1.0510.95
284_Q288_E1.0370.95
221_Q225_N1.0350.95
130_L263_F1.0340.95
222_D225_N1.0330.95
30_D144_I1.0300.95
128_L144_I1.0270.95
194_W199_V1.0220.95
24_T27_L1.0200.95
182_F225_N1.0080.94
97_L100_L1.0060.94
71_D75_Y1.0040.94
151_D231_K0.9990.94
43_C132_A0.9980.94
205_A209_E0.9970.94
61_F136_C0.9940.94
138_G238_K0.9910.94
45_I290_K0.9910.94
42_G273_W0.9850.93
31_I61_F0.9770.93
41_F290_K0.9730.93
266_Y282_V0.9700.93
206_M223_V0.9660.93
253_F257_V0.9650.93
184_S224_L0.9630.93
29_L128_L0.9620.93
73_R89_I0.9610.93
151_D190_N0.9610.93
167_K171_S0.9600.93
62_H65_T0.9590.92
62_H88_K0.9510.92
114_F220_D0.9500.92
42_G290_K0.9450.92
143_L235_A0.9340.91
203_A228_L0.9330.91
135_I266_Y0.9320.91
154_A190_N0.9240.91
285_A289_A0.9240.91
134_I263_F0.9220.91
131_D185_G0.9210.91
181_Y225_N0.9160.91
148_F188_L0.9000.90
113_R220_D0.8910.89
120_F191_T0.8850.89
133_D265_H0.8800.89
152_K193_Q0.8770.89
165_W174_V0.8760.89
54_N59_L0.8710.88
69_G72_D0.8670.88
126_K148_F0.8660.88
36_D111_Y0.8640.88
290_K293_S0.8590.88
49_S135_I0.8540.87
29_L144_I0.8480.87
152_K190_N0.8420.87
185_G265_H0.8420.87
42_G271_K0.8360.86
41_F286_F0.8300.86
68_F72_D0.8260.86
95_D98_R0.8250.85
92_I115_V0.8250.85
65_T68_F0.8250.85
49_S53_Y0.8240.85
155_M232_L0.8220.85
121_I191_T0.8210.85
289_A292_A0.8200.85
126_K151_D0.8170.85
44_G79_L0.8140.85
241_T258_T0.8110.85
205_A208_N0.8090.84
49_S289_A0.7990.84
150_D193_Q0.7960.83
30_D90_Y0.7940.83
117_A200_S0.7930.83
37_K40_L0.7910.83
170_H173_G0.7870.83
109_A112_F0.7860.83
293_S297_N0.7860.83
128_L140_I0.7850.83
206_M227_L0.7850.83
166_E170_H0.7840.82
287_M290_K0.7810.82
282_V285_A0.7800.82
140_I263_F0.7760.82
277_A281_P0.7700.81
68_F73_R0.7650.81
223_V227_L0.7640.81
192_A195_A0.7580.80
81_L84_K0.7550.80
254_I257_V0.7520.80
35_T65_T0.7500.80
129_Y181_Y0.7400.79
48_A83_Y0.7390.79
153_V187_L0.7370.78
36_D129_Y0.7340.78
50_I136_C0.7320.78
224_L232_L0.7270.77
273_W286_F0.7250.77
47_I63_I0.7200.77
75_Y78_A0.7180.77
183_N186_F0.7170.76
151_D154_A0.7160.76
203_A227_L0.7160.76
129_Y220_D0.7150.76
274_H277_A0.7150.76
80_A85_T0.7130.76
129_Y187_L0.7110.76
72_D75_Y0.7110.76
152_K228_L0.7070.75
130_L136_C0.7050.75
158_T183_N0.7050.75
87_I91_L0.7030.75
106_W110_I0.6990.75
51_L83_Y0.6990.75
118_D201_A0.6980.75
116_I127_V0.6940.74
40_L72_D0.6930.74
43_C47_I0.6920.74
57_S139_T0.6890.74
216_I226_M0.6870.73
78_A81_L0.6860.73
114_F203_A0.6850.73
111_Y219_P0.6830.73
271_K274_H0.6780.72
131_D224_L0.6770.72
144_I195_A0.6770.72
98_R108_H0.6760.72
146_F233_I0.6760.72
286_F290_K0.6750.72
122_N196_A0.6730.72
148_F233_I0.6690.71
115_V204_I0.6660.71
147_S150_D0.6640.71
115_V223_V0.6640.71
129_Y184_S0.6630.71
89_I93_N0.6630.71
80_A83_Y0.6630.71
53_Y76_F0.6620.71
44_G72_D0.6610.70
108_H186_F0.6610.70
74_K77_D0.6590.70
105_N219_P0.6570.70
150_D231_K0.6560.70
39_F132_A0.6550.70
48_A292_A0.6510.69
169_A286_F0.6510.69
182_F187_L0.6460.69
121_I124_A0.6450.69
39_F181_Y0.6380.68
33_Y50_I0.6380.68
168_R172_L0.6370.68
84_K89_I0.6370.68
61_F85_T0.6360.68
43_C65_T0.6350.67
129_Y225_N0.6350.67
50_I300_L0.6330.67
147_S151_D0.6320.67
47_I50_I0.6310.67
126_K144_I0.6310.67
193_Q231_K0.6310.67
51_L79_L0.6310.67
33_Y132_A0.6230.66
116_I129_Y0.6230.66
202_R205_A0.6190.65
133_D271_K0.6170.65
184_S206_M0.6170.65
183_N224_L0.6160.65
33_Y47_I0.6160.65
154_A159_E0.6150.65
153_V228_L0.6140.65
239_Y290_K0.6100.64
49_S136_C0.6090.64
64_F116_I0.6080.64
44_G76_F0.6050.64
206_M216_I0.6050.64
291_N297_N0.6040.64
62_H124_A0.6020.63
53_Y97_L0.6010.63
285_A288_E0.6010.63
76_F80_A0.6000.63
295_W299_A0.6000.63
260_D288_E0.5990.63
39_F44_G0.5960.63
85_T239_Y0.5950.62
185_G240_N0.5920.62
27_L125_P0.5920.62
143_L188_L0.5920.62
79_L83_Y0.5890.62
112_F286_F0.5870.61
48_A293_S0.5860.61
34_G127_V0.5860.61
126_K146_F0.5850.61
46_S136_C0.5830.61
175_A229_A0.5820.61
158_T242_Q0.5810.61
129_Y186_F0.5810.61
29_L140_I0.5810.61
189_I195_A0.5790.60
48_A51_L0.5780.60
28_C60_C0.5770.60
56_G139_T0.5760.60
138_G260_D0.5750.60
84_K87_I0.5740.60
238_K256_P0.5730.60
35_T68_F0.5680.59
218_H222_D0.5680.59
197_Q259_N0.5660.59
143_L239_Y0.5650.58
75_Y295_W0.5650.58
144_I160_G0.5630.58
107_T219_P0.5600.58
79_L139_T0.5600.58
70_D77_D0.5600.58
291_N296_K0.5590.58
159_E242_Q0.5590.58
171_S174_V0.5590.58
135_I289_A0.5580.58
185_G224_L0.5580.58
194_W197_Q0.5570.57
35_T42_G0.5560.57
88_K120_F0.5550.57
168_R173_G0.5540.57
96_R99_S0.5530.57
87_I92_I0.5520.57
39_F105_N0.5520.57
199_V203_A0.5520.57
199_V228_L0.5510.57
165_W172_L0.5510.57
188_L225_N0.5500.56
237_I242_Q0.5490.56
45_I293_S0.5490.56
165_W168_R0.5490.56
245_L248_Q0.5490.56
76_F295_W0.5470.56
290_K296_K0.5470.56
97_L115_V0.5460.56
42_G132_A0.5460.56
64_F92_I0.5430.56
76_F87_I0.5420.55
241_T282_V0.5420.55
144_I287_M0.5390.55
133_D185_G0.5390.55
183_N265_H0.5380.55
38_N67_Y0.5380.55
29_L32_A0.5370.55
94_G201_A0.5360.55
88_K91_L0.5350.54
78_A126_K0.5330.54
241_T261_T0.5310.54
266_Y285_A0.5310.54
183_N267_I0.5300.54
202_R227_L0.5300.54
36_D130_L0.5290.54
95_D99_S0.5270.53
237_I257_V0.5270.53
66_D108_H0.5260.53
236_D239_Y0.5250.53
71_D74_K0.5250.53
125_P190_N0.5230.53
187_L224_L0.5230.53
105_N261_T0.5220.53
223_V268_G0.5200.52
208_N223_V0.5200.52
110_I223_V0.5200.52
63_I270_T0.5190.52
78_A196_A0.5190.52
87_I90_Y0.5190.52
70_D147_S0.5170.52
125_P281_P0.5170.52
64_F300_L0.5160.52
137_Q260_D0.5160.52
111_Y181_Y0.5150.52
112_F115_V0.5150.52
74_K82_Q0.5150.52
243_F246_N0.5150.52
89_I94_G0.5150.52
194_W283_S0.5150.52
159_E180_G0.5140.52
29_L34_G0.5130.51
139_T150_D0.5120.51
52_K127_V0.5100.51
31_I34_G0.5100.51
98_R259_N0.5080.51
168_R174_V0.5070.51
119_Y149_P0.5060.50
251_E254_I0.5050.50
155_M184_S0.5040.50
295_W298_T0.5030.50
25_E63_I0.5020.50
265_H273_W0.5020.50
129_Y182_F0.5010.50
240_N265_H0.5000.50
158_T266_Y0.5000.50
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1g9rA 1 0.7788 100 0.348 Contact Map
3tztA 2 0.6844 100 0.353 Contact Map
3u2uA 2 0.7109 100 0.492 Contact Map
4uegA 4 0.7109 100 0.494 Contact Map
1ll2A 2 0.7109 100 0.496 Contact Map
4aylA 1 0.5841 95.8 0.917 Contact Map
2p6wA 1 0.5811 95.3 0.92 Contact Map
3iohA 2 0.7021 94.1 0.926 Contact Map
1o7qA 1 0.7286 93.4 0.927 Contact Map
1xhbA 1 0.8171 89.4 0.935 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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