GREMLIN Database
LEP4 - Type 4 prepilin-like proteins leader peptide-processing enzyme
UniProt: P25960 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11359
Length: 225 (215)
Sequences: 2703 (2138)
Seq/√Len: 145.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
30_E45_R4.9661.00
54_T96_R4.5481.00
16_D25_H3.7281.00
90_L100_L3.1721.00
108_L164_A3.0631.00
66_A85_C2.7411.00
102_D160_K2.6151.00
165_A177_I2.6001.00
30_E46_Q2.5611.00
86_F104_L2.4771.00
84_F214_M2.4501.00
156_Y159_F2.3931.00
24_Y46_Q2.3791.00
24_Y30_E2.3701.00
11_V15_A2.2551.00
12_G15_A2.2171.00
91_S210_A2.1901.00
79_A115_F2.1491.00
62_A88_L2.1201.00
37_F53_D2.0721.00
145_V149_C2.0701.00
30_E40_V2.0151.00
186_I189_A1.9891.00
62_A66_A1.9771.00
10_L18_F1.8941.00
188_Y208_W1.8441.00
21_A49_Y1.8231.00
35_L39_Q1.8021.00
124_L128_V1.7901.00
77_T81_F1.7871.00
27_S30_E1.7471.00
145_V153_G1.7241.00
87_V210_A1.7101.00
165_A213_S1.7101.00
69_L77_T1.6831.00
26_L31_D1.6561.00
53_D57_L1.6411.00
26_L46_Q1.6401.00
177_I212_G1.6271.00
184_G215_I1.6171.00
69_L84_F1.5881.00
6_P10_L1.5881.00
66_A81_F1.5661.00
212_G216_N1.5371.00
27_S45_R1.5351.00
24_Y27_S1.5291.00
100_L105_T1.5151.00
80_L217_L1.4981.00
34_A60_C1.4851.00
41_L48_K1.4801.00
32_K36_T1.4361.00
61_V92_V1.4321.00
100_L206_G1.4231.00
65_I84_F1.4211.00
134_G174_L1.4121.00
62_A85_C1.4071.00
82_L111_L1.3771.00
98_Q202_T1.3691.00
70_A77_T1.3511.00
58_I92_V1.3401.00
54_T92_V1.3271.00
11_V14_I1.3201.00
101_P160_K1.3181.00
162_L209_L1.3051.00
149_C153_G1.2971.00
181_A209_L1.2961.00
182_S186_I1.2891.00
142_Y156_Y1.2891.00
27_S46_Q1.2871.00
142_Y152_E1.2841.00
198_R201_T1.2821.00
20_N55_V1.2751.00
18_F23_A1.2531.00
92_V96_R1.2471.00
52_H55_V1.2441.00
173_T177_I1.2401.00
186_I193_Q1.2311.00
123_D126_D1.2271.00
20_N51_W1.2261.00
184_G208_W1.2121.00
140_C144_G1.2091.00
65_I88_L1.2001.00
135_Y159_F1.1981.00
100_L161_L1.1961.00
112_G168_W1.1751.00
31_D40_V1.1631.00
57_L61_V1.1601.00
129_Y133_A1.1471.00
173_T216_N1.1170.99
192_S196_Q1.1170.99
130_G134_G1.1050.99
18_F26_L1.1040.99
162_L177_I1.1030.99
89_T104_L1.1000.99
83_Y213_S1.0980.99
135_Y139_W1.0920.99
16_D27_S1.0870.99
180_I212_G1.0780.99
84_F218_G1.0720.99
143_W146_W1.0620.99
142_Y159_F1.0610.99
101_P104_L1.0570.99
185_G208_W1.0550.99
138_L142_Y1.0450.99
34_A56_P1.0440.99
52_H56_P1.0430.99
131_A167_A1.0400.99
90_L104_L1.0370.99
117_A129_Y1.0300.99
29_L39_Q1.0210.99
134_G166_G1.0030.99
109_L163_A1.0020.99
114_V118_Q0.9830.98
54_T58_I0.9810.98
44_F47_K0.9720.98
186_I190_I0.9570.98
63_A67_C0.9410.98
161_L206_G0.9410.98
90_L105_T0.9340.98
86_F108_L0.9210.98
19_V22_I0.9180.98
185_G189_A0.9130.98
190_I194_L0.9130.98
88_L92_V0.9120.97
215_I219_Y0.9090.97
98_Q204_A0.8990.97
102_D105_T0.8850.97
26_L30_E0.8840.97
181_A205_F0.8840.97
83_Y165_A0.8820.97
176_M216_N0.8810.97
169_C173_T0.8720.97
22_I47_K0.8720.97
56_P60_C0.8650.97
178_L182_S0.8600.96
145_V151_K0.8580.96
69_L80_L0.8480.96
169_C216_N0.8430.96
28_P31_D0.8400.96
112_G164_A0.8390.96
156_Y160_K0.8360.96
30_E36_T0.8240.95
105_T164_A0.8200.95
15_A18_F0.8150.95
132_V135_Y0.8130.95
53_D56_P0.8110.95
66_A69_L0.8080.95
130_G166_G0.8070.95
179_L182_S0.8070.95
79_A168_W0.8000.95
103_K107_P0.8000.95
65_I214_M0.7950.94
214_M218_G0.7920.94
84_F217_L0.7850.94
116_N127_A0.7820.94
29_L45_R0.7770.94
155_G158_D0.7680.93
146_W152_E0.7680.93
210_A213_S0.7660.93
154_L182_S0.7650.93
93_I96_R0.7630.93
138_L178_L0.7600.93
139_W143_W0.7570.93
177_I213_S0.7570.93
83_Y112_G0.7530.93
184_G211_L0.7520.93
8_F93_I0.7490.93
99_L160_K0.7450.92
93_I97_T0.7430.92
177_I181_A0.7420.92
32_K39_Q0.7390.92
114_V132_V0.7380.92
74_P77_T0.7340.92
87_V161_L0.7320.92
36_T40_V0.7320.92
106_L110_W0.7310.92
37_F42_V0.7300.92
122_I126_D0.7250.91
8_F97_T0.7240.91
116_N167_A0.7190.91
24_Y45_R0.7180.91
51_W59_L0.7170.91
64_A68_A0.7160.91
186_I192_S0.7140.91
19_V47_K0.7090.90
180_I184_G0.7060.90
142_Y154_L0.7050.90
102_D106_L0.7040.90
25_H30_E0.7040.90
177_I216_N0.7020.90
140_C143_W0.7020.90
68_A71_P0.7000.90
98_Q203_I0.7000.90
80_L121_L0.6990.90
162_L182_S0.6870.89
193_Q196_Q0.6860.89
99_L102_D0.6830.89
126_D171_W0.6820.89
58_I88_L0.6820.89
72_F80_L0.6780.88
81_F85_C0.6760.88
61_V88_L0.6760.88
7_L10_L0.6720.88
47_K59_L0.6700.88
184_G212_G0.6670.87
94_D158_D0.6660.87
149_C152_E0.6650.87
126_D172_Q0.6630.87
90_L206_G0.6610.87
32_K35_L0.6540.86
14_I18_F0.6520.86
26_L35_L0.6510.86
40_V45_R0.6510.86
107_P167_A0.6490.86
105_T167_A0.6480.86
191_V194_L0.6480.86
69_L81_F0.6460.86
130_G133_A0.6400.85
68_A218_G0.6380.85
116_N123_D0.6370.85
137_V178_L0.6370.85
181_A212_G0.6370.85
139_W156_Y0.6360.85
69_L218_G0.6350.85
146_W150_H0.6340.85
10_L13_F0.6330.85
70_A74_P0.6330.85
72_F77_T0.6330.85
169_C177_I0.6300.84
194_L197_R0.6300.84
192_S197_R0.6290.84
193_Q199_T0.6290.84
196_Q201_T0.6280.84
191_V195_L0.6250.84
189_A203_I0.6240.84
142_Y145_V0.6240.84
146_W153_G0.6200.83
161_L210_A0.6200.83
93_I207_P0.6190.83
83_Y168_W0.6170.83
129_Y171_W0.6140.83
65_I69_L0.6130.83
189_A193_Q0.6120.83
50_A53_D0.6100.82
100_L157_G0.6100.82
137_V140_C0.6100.82
68_A214_M0.6070.82
115_F119_Y0.6060.82
33_T59_L0.6020.82
90_L101_P0.6010.81
113_L127_A0.5990.81
166_G170_G0.5990.81
91_S97_T0.5980.81
16_D28_P0.5980.81
11_V18_F0.5970.81
22_I44_F0.5960.81
7_L12_G0.5950.81
131_A163_A0.5910.80
124_L130_G0.5910.80
93_I104_L0.5910.80
122_I125_H0.5890.80
72_F75_I0.5860.80
26_L29_L0.5840.80
139_W159_F0.5820.79
18_F34_A0.5790.79
13_F18_F0.5770.79
137_V141_V0.5770.79
17_Y59_L0.5770.79
141_V145_V0.5750.79
94_D157_G0.5740.78
104_L164_A0.5730.78
160_K207_P0.5720.78
83_Y209_L0.5700.78
135_Y163_A0.5700.78
154_L159_F0.5660.78
71_P74_P0.5640.77
199_T203_I0.5620.77
197_R201_T0.5620.77
28_P32_K0.5600.77
93_I101_P0.5590.77
108_L135_Y0.5580.77
188_Y191_V0.5560.76
89_T93_I0.5550.76
135_Y156_Y0.5540.76
169_C217_L0.5540.76
179_L183_L0.5520.76
62_A81_F0.5490.75
87_V165_A0.5480.75
186_I208_W0.5470.75
136_G140_C0.5460.75
116_N121_L0.5450.75
192_S198_R0.5450.75
154_L205_F0.5430.75
108_L112_G0.5430.75
177_I209_L0.5420.74
130_G170_G0.5390.74
185_G205_F0.5360.74
84_F88_L0.5340.73
176_M180_I0.5310.73
138_L153_G0.5300.73
157_G206_G0.5280.73
82_L191_V0.5270.72
142_Y146_W0.5260.72
85_C89_T0.5260.72
143_W156_Y0.5250.72
200_I203_I0.5250.72
184_G188_Y0.5250.72
183_L187_G0.5250.72
193_Q201_T0.5230.72
113_L124_L0.5210.72
138_L174_L0.5190.71
107_P111_L0.5170.71
135_Y162_L0.5150.71
19_V44_F0.5150.71
124_L127_A0.5120.70
174_L177_I0.5100.70
154_L208_W0.5100.70
9_I97_T0.5070.70
43_H48_K0.5030.69
82_L115_F0.5010.69
130_G138_L0.5010.69
57_L60_C0.5000.68
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3s0xA 1 0.6578 99.5 0.739 Contact Map
3j20Y 1 0.2089 51.6 0.937 Contact Map
1twfB 2 0.3156 39.2 0.941 Contact Map
1zvfA 2 0.2622 38.4 0.942 Contact Map
4c2mB 1 0.3244 32.1 0.944 Contact Map
3e2iA 3 0.2133 31.5 0.944 Contact Map
4gizC 1 0.2267 27.9 0.946 Contact Map
2fiyA 1 0.5156 24.2 0.947 Contact Map
1twfL 1 0.1822 22.9 0.948 Contact Map
4aybP 1 0.1956 22.7 0.948 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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