GREMLIN Database
MNMA - tRNA-specific 2-thiouridylase MnmA
UniProt: P25745 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11344
Length: 368 (346)
Sequences: 3662 (2073)
Seq/√Len: 111.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
305_R334_D3.8861.00
89_E94_R3.7361.00
220_K270_I3.4901.00
223_T227_D3.4731.00
23_W185_L3.4501.00
183_E191_K3.3101.00
7_K31_Q3.2851.00
131_R172_E2.8751.00
220_K228_E2.8571.00
220_K231_E2.8341.00
262_V274_A2.6871.00
224_V281_R2.6661.00
290_Q361_E2.6521.00
180_K184_D2.6471.00
286_G330_E2.6081.00
265_D268_N2.5961.00
177_Q180_K2.5351.00
218_P231_E2.5241.00
308_V346_A2.4671.00
133_A142_L2.3631.00
27_Q184_D2.3311.00
222_I270_I2.2441.00
289_A346_A2.2411.00
322_K330_E2.2021.00
140_R353_V2.1971.00
36_F69_L2.0981.00
235_L271_L2.0881.00
144_G152_S2.0761.00
57_D192_K2.0401.00
303_T322_K2.0291.00
292_L348_F2.0271.00
143_R309_K2.0051.00
250_G259_W1.9831.00
265_D270_I1.9491.00
287_L346_A1.9461.00
286_G332_I1.9351.00
131_R145_L1.9311.00
19_S189_T1.9301.00
85_L101_L1.9271.00
308_V319_C1.9231.00
221_I273_V1.8891.00
180_K183_E1.8691.00
205_K208_E1.8661.00
131_R171_G1.8511.00
331_V348_F1.8451.00
79_W84_E1.8181.00
9_I121_A1.8091.00
156_Y345_S1.8061.00
33_E70_H1.8001.00
159_S162_Q1.7961.00
257_E275_Q1.7701.00
331_V346_A1.7531.00
174_E177_Q1.7461.00
306_C319_C1.7391.00
158_L162_Q1.7261.00
133_A140_R1.7201.00
307_T318_P1.7001.00
224_V262_V1.6721.00
317_I337_V1.6711.00
322_K332_I1.6481.00
318_P334_D1.6261.00
263_D272_V1.6201.00
64_K188_V1.6201.00
345_S359_I1.6171.00
25_L125_A1.6161.00
156_Y357_G1.6151.00
309_K316_D1.6071.00
305_R320_T1.5911.00
284_S340_V1.5871.00
34_G67_I1.5791.00
104_K157_T1.5731.00
305_R318_P1.5721.00
10_V21_S1.5681.00
276_G338_A1.5431.00
12_M19_S1.5361.00
156_Y359_I1.5311.00
148_N176_P1.5281.00
197_G204_R1.5241.00
22_A67_I1.5241.00
132_R139_S1.5171.00
275_Q281_R1.4851.00
27_Q185_L1.4791.00
235_L264_K1.4371.00
139_S160_H1.4221.00
265_D272_V1.4011.00
143_R316_D1.3961.00
88_A91_K1.3931.00
173_L178_V1.3781.00
9_I35_L1.3731.00
79_W213_Y1.3681.00
88_A92_A1.3671.00
58_A69_L1.3591.00
288_I328_R1.3501.00
179_R194_D1.3451.00
306_C355_L1.3431.00
109_K113_E1.3401.00
12_M34_G1.3401.00
307_T316_D1.3391.00
321_V348_F1.3381.00
320_T334_D1.3381.00
160_H295_V1.3301.00
289_A358_G1.3181.00
100_I345_S1.3171.00
207_R233_Q1.3161.00
181_I185_L1.3130.99
23_W27_Q1.3110.99
287_L333_F1.3090.99
228_E231_E1.3070.99
243_R258_P1.3060.99
6_K122_D1.3060.99
223_T229_I1.3030.99
304_M321_V1.2950.99
85_L89_E1.2860.99
288_I330_E1.2760.99
131_R352_E1.2660.99
81_N105_E1.2650.99
222_I268_N1.2590.99
142_L352_E1.2590.99
84_E88_A1.2450.99
229_I273_V1.2360.99
135_V138_K1.2300.99
20_V181_I1.2270.99
221_I271_L1.2250.99
208_E212_R1.2220.99
8_V30_Y1.2170.99
76_A80_D1.2150.99
7_K33_E1.2120.99
203_E208_E1.2110.99
250_G256_E1.2090.99
264_K271_L1.2070.99
26_Q66_G1.2060.99
323_A329_I1.2030.99
283_M338_A1.2000.99
114_F118_D1.1960.99
142_L353_V1.1940.99
59_Q63_D1.1940.99
287_L340_V1.1930.99
19_S61_V1.1900.99
222_I272_V1.1890.99
345_S357_G1.1880.99
9_I33_E1.1860.99
222_I228_E1.1850.99
292_L329_I1.1770.99
303_T323_A1.1760.99
6_K123_Y1.1720.99
276_G283_M1.1650.99
333_F337_V1.1620.99
139_S164_A1.1610.99
23_W181_I1.1610.99
8_V167_L1.1440.99
285_V332_I1.1430.99
173_L181_I1.1360.99
248_I273_V1.1310.98
111_F124_I1.1200.98
26_Q65_L1.1190.98
320_T332_I1.1160.98
324_L327_D1.1110.98
143_R307_T1.1100.98
146_D174_E1.1080.98
329_I348_F1.1080.98
138_K160_H1.0990.98
309_K312_Y1.0980.98
33_E121_A1.0920.98
134_D139_S1.0920.98
218_P233_Q1.0910.98
292_L358_G1.0860.98
177_Q184_D1.0820.98
324_L330_E1.0700.98
294_W300_F1.0640.98
55_L69_L1.0560.98
8_V123_Y1.0550.98
123_Y167_L1.0510.98
6_K30_Y1.0430.97
134_D137_G1.0430.97
256_E259_W1.0400.97
94_R290_Q1.0340.97
211_G237_Y1.0300.97
325_D328_R1.0270.97
224_V274_A1.0090.97
49_C54_D1.0080.97
324_L328_R1.0010.97
328_R361_E0.9920.97
303_T320_T0.9910.97
144_G171_G0.9900.96
131_R142_L0.9880.96
221_I229_I0.9840.96
207_R237_Y0.9810.96
36_F55_L0.9780.96
161_E164_A0.9740.96
223_T259_W0.9660.96
55_L71_T0.9660.96
170_V178_V0.9650.96
297_R300_F0.9600.96
89_E92_A0.9550.96
244_K258_P0.9540.96
145_L172_E0.9540.96
156_Y347_V0.9530.96
59_Q69_L0.9500.96
350_N353_V0.9440.95
144_G170_V0.9440.95
287_L331_V0.9430.95
110_A113_E0.9430.95
207_R210_L0.9390.95
22_A34_G0.9360.95
11_G111_F0.9360.95
64_K186_G0.9350.95
144_G147_S0.9320.95
306_C348_F0.9310.95
275_Q280_P0.9230.95
202_G208_E0.9210.95
152_S312_Y0.9170.95
289_A348_F0.9120.94
87_L214_L0.9100.94
181_I184_D0.9090.94
143_R152_S0.8960.94
33_E68_E0.8910.94
20_V178_V0.8900.94
100_I156_Y0.8860.94
20_V182_A0.8850.94
287_L308_V0.8840.93
314_Q317_I0.8780.93
145_L174_E0.8760.93
78_Y99_D0.8670.93
306_C321_V0.8620.93
87_L213_Y0.8610.93
138_K297_R0.8590.92
80_D84_E0.8520.92
310_T314_Q0.8520.92
56_A59_Q0.8480.92
12_M62_C0.8390.92
147_S174_E0.8360.91
158_L295_V0.8350.91
287_L310_T0.8340.91
60_A63_D0.8320.91
141_L354_C0.8310.91
248_I259_W0.8310.91
154_F313_R0.8260.91
57_D189_T0.8260.91
108_F166_S0.8240.91
131_R170_V0.8230.91
250_G273_V0.8180.91
11_G32_V0.8170.90
41_E75_A0.8160.90
86_F90_Y0.8100.90
159_S164_A0.8100.90
209_F213_Y0.8080.90
304_M350_N0.8050.90
124_I166_S0.8000.90
235_L266_V0.7960.89
189_T192_K0.7960.89
35_L121_A0.7920.89
347_V354_C0.7890.89
219_G232_H0.7880.89
16_V182_A0.7860.89
58_A62_C0.7850.89
130_V152_S0.7820.88
285_V336_P0.7800.88
294_W356_G0.7790.88
259_W273_V0.7770.88
162_Q166_S0.7740.88
23_W26_Q0.7740.88
300_F304_M0.7730.88
105_E109_K0.7690.88
157_T345_S0.7680.88
179_R189_T0.7650.87
210_L214_L0.7640.87
77_E110_A0.7640.87
7_K120_G0.7630.87
21_S170_V0.7610.87
229_I248_I0.7610.87
288_I324_L0.7570.87
308_V333_F0.7550.87
348_F356_G0.7540.87
126_T198_I0.7520.86
57_D179_R0.7520.86
40_W83_F0.7520.86
310_T337_V0.7520.86
38_K73_N0.7500.86
138_K298_E0.7470.86
202_G205_K0.7470.86
217_Q269_N0.7420.86
132_R163_I0.7380.85
310_T333_F0.7360.85
38_K75_A0.7360.85
329_I358_G0.7360.85
72_V114_F0.7330.85
23_W65_L0.7330.85
24_L173_L0.7310.85
319_C348_F0.7290.85
262_V281_R0.7280.85
145_L171_G0.7270.85
222_I229_I0.7270.85
134_D140_R0.7260.84
24_L181_I0.7250.84
337_V340_V0.7190.84
70_H119_L0.7160.84
35_L111_F0.7150.84
289_A329_I0.7120.83
83_F213_Y0.7110.83
100_I157_T0.7110.83
136_D140_R0.7100.83
312_Y316_D0.7090.83
143_R147_S0.7060.83
39_N78_Y0.7050.83
221_I235_L0.7050.83
133_A172_E0.7020.82
9_I115_A0.7020.82
40_W209_F0.6990.82
158_L163_I0.6980.82
249_G256_E0.6980.82
152_S354_C0.6980.82
24_L172_E0.6960.82
42_E75_A0.6930.82
82_V198_I0.6900.81
16_V190_A0.6890.81
289_A308_V0.6890.81
148_N313_R0.6870.81
261_V271_L0.6860.81
283_M339_A0.6850.81
25_L167_L0.6850.81
92_A290_Q0.6850.81
233_Q238_H0.6830.81
179_R192_K0.6820.81
131_R144_G0.6790.80
78_Y202_G0.6790.80
160_H164_A0.6790.80
35_L72_V0.6790.80
238_H246_L0.6780.80
170_V174_E0.6770.80
274_A281_R0.6760.80
99_D197_G0.6730.80
20_V185_L0.6730.80
114_F119_L0.6710.80
39_N197_G0.6700.79
291_Q327_D0.6700.79
37_M74_F0.6650.79
95_T239_T0.6650.79
321_V329_I0.6630.79
16_V194_D0.6630.79
132_R168_F0.6620.79
12_M61_V0.6620.79
314_Q337_V0.6600.78
83_F209_F0.6600.78
8_V32_V0.6590.78
144_G312_Y0.6560.78
160_H163_I0.6560.78
250_G257_E0.6550.78
8_V25_L0.6500.77
97_N100_I0.6500.77
224_V272_V0.6490.77
243_R260_Y0.6480.77
25_L28_Q0.6480.77
36_F58_A0.6450.77
259_W275_Q0.6450.77
263_D284_S0.6450.77
226_G272_V0.6420.77
289_A292_L0.6420.77
318_P335_E0.6410.76
300_F323_A0.6410.76
58_A61_V0.6400.76
263_D268_N0.6400.76
240_L264_K0.6370.76
112_L162_Q0.6360.76
157_T357_G0.6360.76
309_K313_R0.6350.76
220_K265_D0.6340.76
25_L32_V0.6330.76
295_V356_G0.6330.76
125_A167_L0.6320.75
207_R236_M0.6310.75
185_L188_V0.6300.75
132_R166_S0.6290.75
321_V331_V0.6270.75
60_A64_K0.6270.75
11_G124_I0.6270.75
8_V125_A0.6260.75
276_G280_P0.6230.74
294_W323_A0.6230.74
294_W329_I0.6220.74
22_A65_L0.6190.74
83_F87_L0.6180.74
77_E106_I0.6170.74
39_N99_D0.6160.74
228_E270_I0.6150.73
317_I335_E0.6140.73
301_T323_A0.6110.73
305_R350_N0.6100.73
78_Y83_F0.6090.73
130_V158_L0.6080.73
249_G258_P0.6080.73
59_Q70_H0.6070.72
143_R352_E0.6060.72
97_N312_Y0.6050.72
211_G216_A0.6050.72
208_E211_G0.6050.72
54_D57_D0.6040.72
233_Q247_G0.6040.72
201_I209_F0.6020.72
235_L269_N0.6020.72
63_D66_G0.6010.72
60_A188_V0.6000.72
13_S17_D0.5980.71
24_L28_Q0.5970.71
219_G234_G0.5950.71
79_W83_F0.5940.71
39_N74_F0.5930.71
115_A119_L0.5910.70
307_T350_N0.5890.70
147_S152_S0.5880.70
294_W299_P0.5870.70
264_K310_T0.5860.70
99_D202_G0.5860.70
11_G25_L0.5860.70
115_A124_I0.5850.70
221_I250_G0.5840.70
39_N313_R0.5840.70
146_D154_F0.5830.69
56_A60_A0.5820.69
112_L124_I0.5800.69
139_S158_L0.5800.69
55_L59_Q0.5790.69
302_G323_A0.5790.69
77_E81_N0.5790.69
37_M77_E0.5790.69
62_C69_L0.5780.69
16_V189_T0.5760.69
19_S34_G0.5730.68
196_T204_R0.5730.68
308_V348_F0.5720.68
179_R190_A0.5720.68
28_Q172_E0.5720.68
91_K210_L0.5720.68
154_F168_F0.5710.68
299_P323_A0.5710.68
101_L104_K0.5700.68
306_C331_V0.5700.68
262_V273_V0.5660.67
12_M22_A0.5640.67
211_G236_M0.5620.67
16_V187_L0.5620.67
294_W358_G0.5620.67
319_C331_V0.5610.66
53_A56_A0.5600.66
223_T250_G0.5570.66
250_G260_Y0.5550.66
240_L340_V0.5550.66
137_G160_H0.5550.66
37_M201_I0.5550.66
285_V335_E0.5540.66
7_K121_A0.5540.66
300_F303_T0.5540.66
197_G206_F0.5520.65
159_S166_S0.5510.65
257_E276_G0.5510.65
160_H293_H0.5510.65
259_W264_K0.5470.65
54_D78_Y0.5450.64
134_D138_K0.5440.64
100_I105_E0.5430.64
31_Q68_E0.5420.64
130_V166_S0.5410.64
148_N174_E0.5410.64
160_H297_R0.5380.63
132_R158_L0.5380.63
210_L236_M0.5360.63
307_T351_G0.5360.63
107_K154_F0.5360.63
62_C67_I0.5350.63
238_H269_N0.5340.63
284_S339_A0.5330.63
54_D192_K0.5330.63
346_A360_I0.5300.62
91_K214_L0.5300.62
12_M63_D0.5290.62
64_K187_L0.5280.62
264_K314_Q0.5270.62
41_E79_W0.5260.62
130_V354_C0.5260.62
245_G258_P0.5260.62
37_M339_A0.5260.62
146_D176_P0.5250.61
160_H296_D0.5250.61
38_K42_E0.5250.61
183_E190_A0.5240.61
149_K194_D0.5240.61
38_K44_D0.5230.61
15_G18_S0.5230.61
24_L170_V0.5220.61
233_Q237_Y0.5220.61
216_A236_M0.5220.61
33_E69_L0.5220.61
113_E118_D0.5210.61
296_D300_F0.5210.61
101_L105_E0.5200.61
40_W202_G0.5190.61
323_A327_D0.5170.60
141_L295_V0.5170.60
317_I340_V0.5160.60
249_G257_E0.5150.60
52_A55_L0.5140.60
224_V263_D0.5140.60
308_V331_V0.5130.60
140_R352_E0.5110.59
85_L345_S0.5110.59
246_L271_L0.5110.59
198_I201_I0.5110.59
135_V140_R0.5100.59
21_S24_L0.5100.59
13_S18_S0.5100.59
173_L177_Q0.5100.59
201_I206_F0.5090.59
32_V67_I0.5080.59
38_K52_A0.5070.59
35_L124_I0.5060.59
145_L177_Q0.5050.58
202_G354_C0.5050.58
194_D313_R0.5030.58
88_A214_L0.5010.58
305_R321_V0.5000.58
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2derA 1 0.9728 100 0.25 Contact Map
2hmaA 2 0.9701 100 0.27 Contact Map
4nzpA 4 0.8342 100 0.592 Contact Map
4xfjA 4 0.8533 100 0.634 Contact Map
1korA 4 0.8505 100 0.688 Contact Map
1k92A 4 0.8696 100 0.729 Contact Map
3k32A 2 0.5245 100 0.757 Contact Map
2nz2A 4 0.8315 100 0.768 Contact Map
1vl2A 4 0.8505 100 0.775 Contact Map
4kr6A 2 0.5761 100 0.783 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0109 seconds.