GREMLIN Database
FRMA - S-(hydroxymethyl)glutathione dehydrogenase
UniProt: P25437 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG50010
Length: 369 (327)
Sequences: 35149 (23546)
Seq/√Len: 1302.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
33_K69_V3.0391.00
35_T161_H3.0211.00
35_T67_V2.9711.00
349_E352_D2.9661.00
9_F18_V2.8541.00
189_S213_R2.7821.00
31_L69_V2.6231.00
30_V152_L2.5781.00
64_G140_T2.5191.00
82_D154_K2.4921.00
160_N163_H2.4641.00
9_F16_E2.4521.00
207_R233_T2.4471.00
224_F228_R2.3991.00
185_Q188_D2.3771.00
162_E344_T2.3011.00
98_C106_C2.2831.00
345_M356_L2.2621.00
343_H356_L2.2351.00
213_R234_D2.2191.00
83_H155_I2.2141.00
27_K73_E2.1901.00
28_G74_G2.1881.00
33_K67_V2.1761.00
195_L216_A2.1751.00
48_S358_H2.1721.00
225_D229_R2.1591.00
346_S349_E2.1391.00
355_D359_E2.1331.00
33_K144_Y2.1271.00
27_K74_G2.1051.00
77_S149_E2.0971.00
7_V18_V2.0821.00
158_E331_M2.0791.00
79_K82_D2.0691.00
22_V144_Y2.0511.00
283_Q308_V2.0291.00
328_E332_K2.0121.00
83_H161_H1.9651.00
190_V206_A1.9401.00
191_A257_I1.8951.00
65_A161_H1.8791.00
247_D250_L1.8671.00
163_H338_E1.8191.00
179_H325_G1.8051.00
277_A284_S1.8011.00
69_V144_Y1.7981.00
283_Q310_K1.7891.00
26_K29_E1.7861.00
225_D228_R1.7851.00
64_G86_P1.7791.00
226_L229_R1.7731.00
155_I164_V1.7721.00
179_H209_A1.7681.00
24_P70_E1.7611.00
31_L146_V1.7401.00
191_A217_I1.7401.00
345_M353_A1.7391.00
215_I257_I1.7141.00
224_F235_C1.7091.00
352_D355_D1.6941.00
203_V232_A1.6921.00
356_L361_K1.6711.00
213_R233_T1.6591.00
260_T277_A1.6511.00
258_D278_H1.6441.00
221_P225_D1.6381.00
4_R19_E1.6301.00
241_Y247_D1.6291.00
352_D356_L1.6231.00
229_R339_P1.6041.00
70_E80_P1.5961.00
351_N355_D1.5861.00
6_A350_I1.5811.00
78_V154_K1.5781.00
221_P237_N1.5591.00
71_V80_P1.5431.00
34_V64_G1.5341.00
31_L144_Y1.5301.00
155_I161_H1.5261.00
260_T273_A1.5231.00
277_A307_R1.4851.00
4_R348_D1.4851.00
208_Q334_D1.4661.00
72_G75_V1.4631.00
328_E331_M1.4471.00
25_P146_V1.4421.00
193_F273_A1.4421.00
83_H157_P1.4411.00
67_V83_H1.4341.00
171_V201_A1.4301.00
36_H162_E1.4221.00
277_A282_G1.4171.00
28_G148_A1.4171.00
237_N240_D1.4141.00
221_P240_D1.4071.00
219_T238_P1.3961.00
78_V82_D1.3921.00
67_V161_H1.3911.00
6_A17_I1.3901.00
68_V78_V1.3851.00
202_V261_F1.3831.00
141_F354_F1.3811.00
31_L70_E1.3751.00
29_E146_V1.3741.00
25_P31_L1.3721.00
29_E148_A1.3671.00
22_V31_L1.3631.00
4_R143_E1.3431.00
203_V216_A1.3421.00
336_D339_P1.3371.00
5_A20_I1.3251.00
221_P224_F1.3091.00
218_D224_F1.3081.00
247_D251_D1.3011.00
167_L330_A1.2931.00
189_S215_I1.2751.00
30_V78_V1.2661.00
188_D283_Q1.2611.00
182_A259_H1.2581.00
28_G149_E1.2351.00
10_A13_K1.2151.00
246_K250_L1.2061.00
78_V152_L1.1971.00
264_I269_V1.1941.00
275_E302_Q1.1921.00
47_L354_F1.1871.00
228_R235_C1.1581.00
34_V145_T1.1561.00
189_S258_D1.1521.00
193_F217_I1.1451.00
32_I84_V1.1401.00
181_T308_V1.1381.00
39_V353_A1.1371.00
35_T83_H1.1311.00
195_L218_D1.1281.00
224_F237_N1.1211.00
174_G202_V1.1151.00
219_T224_F1.1141.00
7_V20_I1.1091.00
208_Q329_D1.1031.00
69_V80_P1.1021.00
234_D251_D1.1011.00
179_H322_Q1.0991.00
25_P29_E1.0951.00
188_D258_D1.0951.00
159_A164_V1.0901.00
325_G328_E1.0871.00
184_V188_D1.0801.00
188_D259_H1.0801.00
36_H161_H1.0781.00
324_P328_E1.0741.00
355_D358_H1.0651.00
9_F14_P1.0611.00
58_V138_C1.0601.00
37_T140_T1.0591.00
6_A347_L1.0581.00
191_A276_S1.0501.00
32_I66_G1.0411.00
141_F350_I1.0381.00
20_I138_C1.0371.00
107_V279_R1.0351.00
10_A14_P1.0301.00
271_R275_E1.0181.00
171_V326_M1.0171.00
17_I348_D1.0171.00
44_A357_M1.0111.00
20_I59_V1.0051.00
22_V70_E1.0041.00
216_A227_A1.0041.00
35_T65_A1.0031.00
176_G322_Q1.0001.00
362_S365_T0.9981.00
195_L203_V0.9981.00
345_M349_E0.9921.00
219_T239_N0.9921.00
177_A287_I0.9901.00
217_I238_P0.9881.00
273_A284_S0.9821.00
216_A232_A0.9821.00
174_G205_G0.9791.00
7_V59_V0.9771.00
102_K280_G0.9761.00
175_I179_H0.9731.00
184_V211_A0.9701.00
182_A285_V0.9641.00
342_T356_L0.9631.00
37_T141_F0.9631.00
215_I234_D0.9621.00
76_T149_E0.9581.00
147_V152_L0.9581.00
220_N223_K0.9541.00
204_Q336_D0.9541.00
64_G145_T0.9521.00
192_V206_A0.9521.00
33_K143_E0.9511.00
205_G326_M0.9481.00
47_L358_H0.9461.00
193_F269_V0.9301.00
242_D246_K0.9301.00
271_R295_E0.9201.00
252_I255_W0.9181.00
6_A15_L0.9151.00
222_K225_D0.9131.00
203_V214_I0.9051.00
5_A59_V0.9001.00
329_D334_D0.8991.00
242_D247_D0.8971.00
71_V79_K0.8951.00
218_D235_C0.8911.00
228_R234_D0.8851.00
262_E265_G0.8811.00
22_V69_V0.8741.00
260_T284_S0.8721.00
272_A275_E0.8701.00
40_C62_H0.8641.00
3_S22_V0.8641.00
40_C63_E0.8611.00
86_P147_V0.8581.00
226_L363_I0.8581.00
245_I275_E0.8561.00
79_K154_K0.8541.00
206_A211_A0.8511.00
240_D247_D0.8491.00
177_A261_F0.8461.00
362_S366_V0.8421.00
183_K283_Q0.8401.00
30_V149_E0.8391.00
156_N331_M0.8391.00
181_T310_K0.8371.00
325_G329_D0.8371.00
302_Q305_T0.8321.00
215_I236_I0.8291.00
190_V211_A0.8261.00
193_F260_T0.8251.00
245_I272_A0.8231.00
153_A324_P0.8191.00
140_T145_T0.8151.00
223_K363_I0.8151.00
71_V75_V0.8141.00
11_P56_F0.8111.00
329_D333_G0.8101.00
195_L227_A0.8091.00
280_G306_G0.8031.00
266_N269_V0.7991.00
75_V149_E0.7961.00
6_A351_N0.7921.00
303_L309_W0.7921.00
341_V344_T0.7891.00
174_G201_A0.7871.00
178_V206_A0.7821.00
28_G73_E0.7801.00
189_S257_I0.7791.00
267_V271_R0.7791.00
85_I327_V0.7781.00
174_G198_I0.7761.00
24_P31_L0.7731.00
175_I326_M0.7701.00
176_G306_G0.7661.00
84_V152_L0.7641.00
219_T227_A0.7621.00
257_I260_T0.7601.00
174_G178_V0.7591.00
39_V357_M0.7571.00
3_S23_A0.7541.00
244_P248_V0.7541.00
65_A165_C0.7501.00
26_K146_V0.7491.00
320_R324_P0.7481.00
192_V202_V0.7471.00
214_I233_T0.7461.00
28_G72_G0.7441.00
175_I325_G0.7381.00
153_A327_V0.7371.00
38_G63_E0.7351.00
207_R214_I0.7331.00
295_E299_R0.7331.00
286_I309_W0.7281.00
250_L253_N0.7281.00
25_P70_E0.7241.00
157_P331_M0.7241.00
268_N272_A0.7241.00
172_T322_Q0.7191.00
87_L153_A0.7151.00
184_V259_H0.7141.00
107_V255_W0.7111.00
200_L226_L0.7111.00
192_V216_A0.7111.00
82_D157_P0.7101.00
206_A214_I0.7041.00
299_R302_Q0.7031.00
105_L306_G0.7021.00
356_L359_E0.7001.00
271_R297_S0.6991.00
260_T276_S0.6981.00
39_V141_F0.6951.00
4_R17_I0.6951.00
65_A83_H0.6941.00
248_V257_I0.6941.00
163_H336_D0.6931.00
36_H344_T0.6931.00
327_V331_M0.6921.00
245_I276_S0.6891.00
61_G141_F0.6831.00
354_F358_H0.6821.00
294_Q297_S0.6821.00
343_H352_D0.6771.00
83_H158_E0.6751.00
32_I86_P0.6751.00
54_G58_V0.6711.00
279_R305_T0.6701.00
183_K310_K0.6691.00
262_E284_S0.6681.00
153_A323_L0.6681.00
110_R137_G0.6661.00
259_H285_V0.6651.00
321_S325_G0.6651.00
177_A285_V0.6631.00
38_G165_C0.6591.00
17_I347_L0.6581.00
329_D332_K0.6571.00
177_A202_V0.6551.00
170_G201_A0.6551.00
28_G150_V0.6541.00
259_H283_Q0.6471.00
236_I244_P0.6471.00
42_T62_H0.6461.00
303_L307_R0.6441.00
211_A214_I0.6441.00
85_I155_I0.6431.00
23_A146_V0.6421.00
227_A232_A0.6401.00
41_H45_F0.6401.00
172_T315_G0.6401.00
32_I152_L0.6401.00
215_I248_V0.6391.00
204_Q230_F0.6391.00
3_S143_E0.6391.00
321_S324_P0.6361.00
175_I329_D0.6341.00
30_V68_V0.6321.00
183_K308_V0.6291.00
15_L354_F0.6281.00
190_V259_H0.6281.00
44_A358_H0.6271.00
25_P72_G0.6261.00
285_V310_K0.6251.00
257_I276_S0.6231.00
215_I251_D0.6231.00
173_T198_I0.6221.00
268_N271_R0.6221.00
364_R367_I0.6181.00
178_V205_G0.6161.00
202_V287_I0.6161.00
45_F50_D0.6161.00
77_S324_P0.6151.00
303_L311_G0.6151.00
242_D272_A0.6141.00
192_V203_V0.6121.00
267_V297_S0.6091.00
218_D238_P0.6091.00
36_H65_A0.6091.00
40_C43_D0.6051.00
229_R336_D0.6041.00
312_S315_G0.6041.00
77_S154_K0.6031.00
244_P272_A0.6001.00
207_R232_A0.5971.00
159_A330_A0.5951.00
211_A259_H0.5951.00
42_T45_F0.5941.00
3_S142_S0.5941.00
245_I248_V0.5941.00
8_A15_L0.5931.00
264_I288_G0.5931.00
191_A260_T0.5921.00
269_V273_A0.5911.00
289_V304_V0.5901.00
202_V263_C0.5901.00
175_I322_Q0.5901.00
65_A155_I0.5901.00
322_Q325_G0.5901.00
167_L326_M0.5891.00
21_D24_P0.5891.00
224_F238_P0.5881.00
23_A144_Y0.5881.00
200_L227_A0.5871.00
5_A142_S0.5861.00
77_S80_P0.5861.00
252_I256_G0.5851.00
270_M286_I0.5841.00
173_T287_I0.5841.00
217_I236_I0.5841.00
204_Q339_P0.5821.00
154_K157_P0.5801.00
186_P210_K0.5791.00
262_E288_G0.5791.00
77_S320_R0.5781.00
13_K48_S0.5741.00
71_V82_D0.5731.00
204_Q335_I0.5721.00
23_A31_L0.5711.00
209_A325_G0.5711.00
55_V58_V0.5701.00
46_T51_D0.5691.00
323_L327_V0.5681.00
45_F289_V0.5671.00
203_V230_F0.5661.00
218_D223_K0.5661.00
263_C287_I0.5661.00
22_V25_P0.5651.00
222_K361_K0.5651.00
86_P152_L0.5621.00
262_E269_V0.5611.00
170_G197_A0.5591.00
175_I209_A0.5561.00
62_H169_C0.5561.00
271_R302_Q0.5551.00
236_I241_Y0.5541.00
203_V207_R0.5511.00
186_P209_A0.5491.00
228_R233_T0.5491.00
71_V78_V0.5491.00
78_V149_E0.5471.00
251_D255_W0.5461.00
175_I205_G0.5441.00
159_A331_M0.5421.00
300_P303_L0.5411.00
177_A312_S0.5411.00
11_P14_P0.5411.00
90_A151_S0.5391.00
40_C169_C0.5391.00
208_Q335_I0.5391.00
46_T58_V0.5381.00
251_D254_K0.5381.00
189_S256_G0.5381.00
90_A148_A0.5371.00
242_D245_I0.5361.00
218_D227_A0.5341.00
272_A276_S0.5331.00
168_G173_T0.5321.00
201_A340_F0.5321.00
330_A337_L0.5321.00
36_H165_C0.5301.00
29_E137_G0.5291.00
248_V276_S0.5281.00
68_V82_D0.5281.00
111_E116_G0.5271.00
219_T237_N0.5271.00
345_M352_D0.5261.00
324_P327_V0.5251.00
189_S234_D0.5251.00
88_Y314_F0.5241.00
248_V252_I0.5241.00
236_I247_D0.5241.00
357_M366_V0.5231.00
34_V140_T0.5231.00
356_L362_S0.5221.00
3_S144_Y0.5221.00
10_A16_E0.5221.00
190_V261_F0.5211.00
302_Q307_R0.5201.00
193_F276_S0.5191.00
244_P247_D0.5191.00
203_V227_A0.5191.00
183_K209_A0.5161.00
15_L60_L0.5161.00
167_L327_V0.5151.00
7_V138_C0.5141.00
171_V205_G0.5131.00
274_L284_S0.5131.00
34_V37_T0.5131.00
8_A56_F0.5111.00
178_V209_A0.5091.00
156_N164_V0.5081.00
15_L351_N0.5081.00
353_A356_L0.5071.00
273_A277_A0.5071.00
182_A283_Q0.5071.00
304_V311_G0.5061.00
179_H205_G0.5061.00
201_A205_G0.5041.00
42_T46_T0.5031.00
221_P239_N0.5021.00
204_Q208_Q0.5021.00
8_A47_L0.5021.00
85_I88_Y0.5021.00
237_N241_Y0.5011.00
201_A204_Q0.5001.00
59_V138_C0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1cdoA 2 0.9946 100 0.151 Contact Map
2fzwA 2 0.9973 100 0.152 Contact Map
3ukoA 4 0.9946 100 0.154 Contact Map
1p0fA 2 0.9892 100 0.157 Contact Map
1e3iA 2 0.9864 100 0.157 Contact Map
2jhfA 2 0.9946 100 0.158 Contact Map
4rqtA 2 0.9973 100 0.165 Contact Map
1f8fA 4 0.9702 100 0.173 Contact Map
1vj0A 4 0.9485 100 0.192 Contact Map
3ip1A 4 0.9106 100 0.202 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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