GREMLIN Database
MCRA - 5-methylcytosine-specific restriction enzyme A
UniProt: P24200 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10573
Length: 277 (224)
Sequences: 184 (160)
Seq/√Len: 10.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
228_H256_H3.4300.97
226_E253_R3.2600.96
246_A249_P3.0760.95
232_P235_S2.9500.93
254_E260_N2.9210.93
225_L246_A2.8000.91
247_L268_L2.7230.90
202_Q208_E2.5610.87
106_Y109_R2.5260.86
248_C251_C2.4550.85
210_C225_L2.2940.80
215_P225_L2.2920.80
254_E264_L2.2450.79
46_Y49_E2.2170.78
166_I169_T2.1940.77
191_R229_H2.1570.76
226_E256_H2.1520.76
210_C214_A2.1390.75
225_L237_G2.1030.74
210_C237_G2.0450.72
224_Y227_V2.0150.70
229_H239_D2.0080.70
175_G195_V1.9150.66
209_N264_L1.9000.66
205_G215_P1.8550.64
205_G225_L1.8150.62
247_L255_L1.8000.61
200_L258_S1.7790.60
225_L249_P1.7670.60
209_N254_E1.7660.60
216_F228_H1.7630.60
199_I230_V1.7590.59
237_G246_A1.7270.58
64_A127_N1.7180.58
254_E258_S1.7050.57
89_I103_Y1.6970.57
205_G237_G1.6880.56
210_C246_A1.6670.55
249_P253_R1.6380.54
205_G214_A1.6310.53
224_Y228_H1.6290.53
215_P237_G1.6240.53
92_S98_L1.6040.52
216_F256_H1.5720.51
191_R239_D1.5500.50
226_E245_V1.5310.49
211_G271_N1.5270.49
216_F224_Y1.5220.48
64_A71_H1.5100.48
46_Y50_R1.4930.47
96_I100_M1.4760.46
239_D243_N1.4710.46
233_L237_G1.4710.46
210_C215_P1.4630.46
214_A237_G1.4620.45
223_P246_A1.4570.45
205_G246_A1.4520.45
224_Y256_H1.4290.44
216_F226_E1.4060.43
215_P246_A1.3940.42
62_Y69_R1.3930.42
71_H80_I1.3840.42
218_L227_V1.3690.41
205_G210_C1.3600.41
224_Y245_V1.3310.39
252_H256_H1.3300.39
73_H125_L1.3110.39
237_G249_P1.3090.38
214_A225_L1.3080.38
191_R228_H1.3070.38
64_A70_K1.3050.38
205_G233_L1.2950.38
161_R169_T1.2940.38
223_P237_G1.2830.37
66_S69_R1.2780.37
64_A69_R1.2660.37
210_C223_P1.2570.36
208_E258_S1.2560.36
245_V272_I1.2450.36
156_S259_K1.2350.35
62_Y66_S1.2350.35
230_V233_L1.2310.35
204_K247_L1.2250.35
228_H252_H1.2220.35
135_S189_Y1.2170.34
98_L124_I1.2090.34
191_R252_H1.2050.34
62_Y70_K1.2040.34
191_R256_H1.1990.34
154_D158_L1.1920.33
253_R257_Y1.1790.33
263_E267_M1.1580.32
66_S100_M1.1470.31
56_V177_R1.1460.31
243_N250_N1.1310.31
64_A122_K1.1260.31
229_H256_H1.1080.30
267_M271_N1.1000.30
67_S132_Y1.0920.29
87_T131_I1.0910.29
65_S78_R1.0820.29
44_L230_V1.0820.29
229_H252_H1.0760.29
162_V253_R1.0750.29
210_C249_P1.0590.28
116_P127_N1.0580.28
66_S104_Q1.0520.28
225_L233_L1.0510.28
70_K122_K1.0510.28
227_V243_N1.0440.27
222_N230_V1.0370.27
232_P253_R1.0310.27
115_I192_D1.0260.27
210_C236_G1.0170.26
215_P249_P1.0110.26
65_S77_E1.0040.26
92_S124_I0.9970.26
63_L91_N0.9950.26
162_V193_P0.9890.25
64_A116_P0.9860.25
245_V250_N0.9810.25
164_K167_K0.9810.25
64_A77_E0.9800.25
62_Y127_N0.9740.25
253_R256_H0.9670.25
69_R122_K0.9610.24
253_R272_I0.9410.24
186_Q189_Y0.9260.23
193_P197_A0.9230.23
208_E257_Y0.9200.23
191_R226_E0.9190.23
200_L208_E0.9180.23
226_E229_H0.9160.23
120_R123_R0.9140.23
58_Q199_I0.9130.23
94_R133_S0.9090.23
163_S167_K0.9070.23
80_I235_S0.9060.23
196_K227_V0.9020.22
243_N247_L0.9010.22
232_P255_L0.8980.22
44_L92_S0.8950.22
65_S79_K0.8940.22
228_H253_R0.8850.22
225_L236_G0.8810.22
207_C252_H0.8740.22
169_T192_D0.8710.21
71_H232_P0.8650.21
112_R197_A0.8650.21
218_L222_N0.8620.21
112_R167_K0.8610.21
214_A246_A0.8590.21
66_S122_K0.8580.21
232_P244_C0.8540.21
44_L98_L0.8530.21
45_D48_I0.8470.21
114_E258_S0.8320.20
116_P122_K0.8290.20
80_I83_G0.8270.20
94_R266_E0.8270.20
77_E83_G0.8250.20
209_N255_L0.8240.20
94_R97_R0.8200.20
201_Q206_I0.8070.19
208_E255_L0.8070.19
78_R109_R0.8020.19
248_C252_H0.8020.19
162_V219_N0.8000.19
66_S102_G0.7970.19
71_H124_I0.7920.19
146_S149_S0.7890.19
209_N260_N0.7800.19
87_T127_N0.7790.19
233_L257_Y0.7790.19
73_H80_I0.7730.18
127_N131_I0.7710.18
165_L183_E0.7680.18
149_S154_D0.7680.18
66_S127_N0.7660.18
63_L102_G0.7650.18
174_E189_Y0.7610.18
227_V244_C0.7560.18
199_I242_D0.7550.18
81_H103_Y0.7520.18
207_C229_H0.7510.18
71_H122_K0.7480.18
69_R116_P0.7430.18
210_C251_C0.7420.18
189_Y234_S0.7420.18
137_W262_K0.7410.18
203_S216_F0.7400.18
67_S202_Q0.7390.18
122_K129_P0.7380.17
150_Q162_V0.7370.17
76_D79_K0.7350.17
168_K222_N0.7310.17
236_G253_R0.7290.17
66_S116_P0.7240.17
205_G230_V0.7220.17
178_K185_L0.7210.17
69_R125_L0.7200.17
132_Y231_I0.7160.17
215_P223_P0.7160.17
210_C233_L0.7150.17
61_V208_E0.7140.17
207_C251_C0.7130.17
66_S77_E0.7130.17
159_N162_V0.7110.17
209_N240_T0.7100.17
197_A201_Q0.7070.17
64_A73_H0.7060.17
214_A223_P0.7040.17
107_F153_D0.7020.17
185_L212_K0.7000.16
124_I255_L0.7000.16
244_C265_I0.6960.16
88_L106_Y0.6940.16
65_S83_G0.6910.16
47_I51_L0.6900.16
85_Y133_S0.6850.16
100_M208_E0.6820.16
199_I244_C0.6820.16
151_P154_D0.6800.16
94_R124_I0.6780.16
205_G249_P0.6770.16
215_P233_L0.6740.16
155_E197_A0.6740.16
82_P109_R0.6690.16
75_L78_R0.6690.16
178_K183_E0.6660.16
215_P236_G0.6650.16
66_S73_H0.6630.16
190_V234_S0.6620.16
84_E96_I0.6620.16
59_V81_H0.6620.16
224_Y253_R0.6610.15
255_L264_L0.6590.15
153_D174_E0.6590.15
260_N264_L0.6580.15
199_I214_A0.6580.15
73_H247_L0.6570.15
91_N102_G0.6570.15
44_L88_L0.6560.15
154_D164_K0.6540.15
191_R207_C0.6540.15
96_I208_E0.6530.15
63_L90_G0.6520.15
192_D197_A0.6510.15
198_W242_D0.6500.15
69_R102_G0.6490.15
73_H100_M0.6490.15
49_E192_D0.6470.15
152_T161_R0.6460.15
71_H83_G0.6430.15
59_V118_G0.6430.15
58_Q227_V0.6400.15
232_P245_V0.6400.15
60_V127_N0.6390.15
184_R230_V0.6300.15
180_V246_A0.6290.15
209_N247_L0.6290.15
185_L195_V0.6270.15
117_S121_T0.6260.15
238_A268_L0.6250.15
226_E252_H0.6250.15
132_Y136_F0.6220.15
55_G231_I0.6180.14
102_G127_N0.6160.14
87_T260_N0.6150.14
74_S259_K0.6140.14
58_Q117_S0.6100.14
135_S158_L0.6090.14
108_S262_K0.6060.14
177_R217_Y0.6060.14
261_A265_I0.6050.14
67_S208_E0.6030.14
103_Y199_I0.6030.14
137_W153_D0.6000.14
207_C239_D0.5980.14
68_V118_G0.5950.14
113_K174_E0.5940.14
204_K212_K0.5940.14
205_G223_P0.5920.14
207_C248_C0.5890.14
203_S234_S0.5840.14
59_V74_S0.5820.14
53_D64_A0.5780.14
60_V64_A0.5770.14
221_G237_G0.5730.13
200_L226_E0.5720.13
66_S70_K0.5710.13
71_H129_P0.5690.13
244_C256_H0.5690.13
81_H91_N0.5670.13
226_E232_P0.5660.13
195_V220_D0.5660.13
95_D181_E0.5620.13
59_V173_P0.5610.13
116_P169_T0.5600.13
106_Y110_T0.5600.13
62_Y87_T0.5580.13
200_L263_E0.5560.13
91_N104_Q0.5550.13
164_K204_K0.5550.13
65_S127_N0.5540.13
45_D104_Q0.5490.13
164_K169_T0.5470.13
94_R176_S0.5420.13
56_V234_S0.5360.13
55_G58_Q0.5350.13
86_F182_V0.5340.13
210_C213_N0.5340.13
66_S129_P0.5340.13
93_P195_V0.5330.13
195_V271_N0.5320.13
44_L66_S0.5280.12
214_A228_H0.5280.12
159_N170_L0.5270.12
163_S188_V0.5260.12
195_V214_A0.5190.12
206_I270_V0.5170.12
60_V66_S0.5140.12
100_M107_F0.5140.12
54_S111_G0.5130.12
45_D243_N0.5120.12
85_Y94_R0.5110.12
126_I226_E0.5090.12
165_L241_T0.5060.12
241_T268_L0.5060.12
71_H116_P0.5030.12
218_L258_S0.5030.12
230_V242_D0.5020.12
166_I218_L0.5010.12
180_V206_I0.5000.12
100_M125_L0.5000.12
196_K247_L0.5000.12
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4ogeA 1 0.8556 99.3 0.85 Contact Map
4h9dA 3 0.343 99.2 0.857 Contact Map
4un4B 1 0.7617 98.9 0.874 Contact Map
3q0bX 1 0.4801 98.8 0.877 Contact Map
4uhqA 1 0.4657 98.1 0.902 Contact Map
3fdeA 2 0.5957 98.1 0.903 Contact Map
2pb7A 1 0.5523 97.8 0.909 Contact Map
3olnA 1 0.5235 97.6 0.913 Contact Map
7ceiB 1 0.3213 96.8 0.923 Contact Map
4qenA 1 0.8845 96.6 0.925 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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