GREMLIN Database
ACRE - Multidrug export protein AcrE
UniProt: P24180 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10266
Length: 385 (348)
Sequences: 22151 (17665)
Seq/√Len: 946.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
326_N332_E3.2881.00
77_N94_Q3.0911.00
91_S174_K2.9681.00
337_V350_E2.7981.00
43_I357_Q2.6991.00
321_T333_A2.6761.00
87_Q180_S2.5711.00
334_R356_D2.4701.00
122_H126_K2.3811.00
324_I334_R2.3621.00
60_N214_D2.3301.00
309_Q346_W2.3141.00
260_Q278_V2.2681.00
242_N256_K2.2621.00
46_T354_S2.2411.00
94_Q174_K2.1891.00
306_L345_K2.1811.00
257_G277_A2.1671.00
47_A345_K2.1391.00
129_V133_G2.1101.00
128_Y137_I2.0881.00
108_K167_R2.0871.00
79_N94_Q2.0801.00
70_Q172_Y2.0511.00
87_Q90_Q2.0501.00
46_T303_D2.0461.00
91_S176_T2.0021.00
97_P101_Q1.9621.00
146_I150_R1.9571.00
353_K356_D1.9261.00
253_Y285_T1.9251.00
128_Y145_A1.9251.00
244_E256_K1.9121.00
47_A306_L1.9121.00
339_S347_L1.8881.00
85_D182_R1.8831.00
139_Q143_D1.8811.00
184_G206_Q1.8761.00
215_V294_A1.8721.00
79_N91_S1.8541.00
129_V146_I1.8481.00
330_Q371_Q1.8481.00
332_E368_P1.7671.00
244_E254_P1.7651.00
21_I24_C1.7601.00
132_V142_Y1.7491.00
59_T213_V1.7421.00
214_D274_T1.7221.00
243_V277_A1.7091.00
52_K295_R1.7091.00
338_A346_W1.7031.00
88_A180_S1.6941.00
337_V349_S1.6811.00
157_I160_K1.6711.00
339_S349_S1.6551.00
150_R153_D1.6551.00
212_Y276_R1.6521.00
149_A153_D1.6341.00
125_V129_V1.6281.00
132_V139_Q1.6141.00
122_H153_D1.6111.00
215_V277_A1.6011.00
331_V359_I1.6011.00
75_V188_V1.5951.00
325_V372_V1.5651.00
112_A115_E1.5651.00
21_I25_N1.5541.00
115_E160_K1.5271.00
113_K155_A1.5241.00
20_L24_C1.5171.00
242_N258_T1.5171.00
105_D108_K1.5041.00
120_I124_T1.4931.00
340_Q347_L1.4921.00
75_V93_Y1.4771.00
315_T333_A1.4641.00
164_E167_R1.4591.00
44_V305_I1.4551.00
118_A149_A1.4461.00
44_V355_G1.4461.00
247_M292_V1.4451.00
63_R207_Q1.4421.00
141_E144_Q1.4421.00
322_V336_V1.4411.00
48_P302_P1.4341.00
213_V292_V1.4311.00
81_T84_S1.4301.00
324_I356_D1.4221.00
59_T211_I1.4201.00
54_E295_R1.4201.00
309_Q344_D1.4101.00
122_H149_A1.3971.00
216_T274_T1.3971.00
115_E119_A1.3961.00
125_V142_Y1.3941.00
104_Y163_V1.3931.00
111_L115_E1.3911.00
266_V273_I1.3811.00
244_E295_R1.3811.00
301_Q347_L1.3801.00
66_E201_E1.3771.00
144_Q148_D1.3691.00
142_Y146_I1.3621.00
54_E293_R1.3601.00
40_T361_S1.3481.00
125_V149_A1.3461.00
41_V374_A1.3361.00
119_A122_H1.3361.00
92_L203_A1.3311.00
307_I358_V1.3301.00
207_Q210_P1.3241.00
64_I204_T1.3241.00
111_L163_V1.3241.00
101_Q105_D1.3201.00
76_L96_D1.3081.00
73_G95_I1.2951.00
218_S221_D1.2931.00
41_V325_V1.2921.00
42_H360_V1.2811.00
101_Q170_L1.2791.00
41_V357_Q1.2771.00
48_P303_D1.2751.00
258_T278_V1.2711.00
246_V295_R1.2691.00
304_A347_L1.2531.00
160_K164_E1.2521.00
244_E297_D1.2521.00
38_Q373_K1.2511.00
126_K129_V1.2401.00
118_A122_H1.2391.00
183_I203_A1.2371.00
110_E113_K1.2351.00
210_P278_V1.2311.00
177_A205_V1.2301.00
111_L156_V1.2211.00
314_R320_A1.2201.00
66_E204_T1.2091.00
153_D157_I1.2031.00
305_I354_S1.1961.00
100_Y173_T1.1951.00
125_V146_I1.1881.00
108_K112_A1.1851.00
72_S196_N1.1781.00
185_K204_T1.1781.00
104_Y108_K1.1751.00
245_L279_F1.1741.00
336_V348_I1.1671.00
77_N190_E1.1631.00
113_K117_A1.1631.00
261_F276_R1.1601.00
167_R171_A1.1601.00
91_S94_Q1.1511.00
165_S169_N1.1511.00
93_Y203_A1.1401.00
222_F273_I1.1371.00
80_F90_Q1.1341.00
186_S203_A1.1271.00
103_N106_S1.1261.00
119_A123_L1.1191.00
213_V294_A1.1151.00
124_T128_Y1.1121.00
246_V254_P1.1061.00
156_V160_K1.1031.00
124_T144_Q1.1011.00
214_D276_R1.1001.00
130_P134_T1.0981.00
309_Q341_A1.0931.00
325_V357_Q1.0871.00
259_L277_A1.0781.00
126_K130_P1.0771.00
62_Y210_P1.0741.00
342_I345_K1.0731.00
186_S189_T1.0691.00
140_Q144_Q1.0661.00
320_A338_A1.0621.00
129_V142_Y1.0551.00
106_S162_T1.0531.00
163_V167_R1.0531.00
119_A150_R1.0501.00
70_Q74_I1.0491.00
130_P133_G1.0481.00
263_D274_T1.0471.00
324_I332_E1.0461.00
312_V346_W1.0421.00
341_A346_W1.0421.00
121_A152_A1.0401.00
65_A179_I1.0401.00
221_D224_R1.0381.00
213_V245_L1.0341.00
97_P173_T1.0301.00
75_V194_V1.0281.00
81_T186_S1.0231.00
247_M250_G1.0131.00
326_N330_Q1.0111.00
258_T280_P1.0081.00
76_L94_Q1.0081.00
93_Y175_V1.0021.00
82_E185_K1.0001.00
43_I355_G1.0001.00
312_V320_A0.9991.00
78_R190_E0.9951.00
104_Y167_R0.9951.00
118_A156_V0.9921.00
59_T286_L0.9901.00
106_S110_E0.9871.00
117_A120_I0.9851.00
326_N334_R0.9831.00
281_N284_H0.9811.00
246_V293_R0.9761.00
58_R271_G0.9751.00
143_D146_I0.9721.00
118_A153_D0.9701.00
69_P175_V0.9681.00
210_P280_P0.9651.00
59_T292_V0.9561.00
212_Y278_V0.9551.00
115_E156_V0.9531.00
50_E297_D0.9491.00
148_D152_A0.9451.00
135_K139_Q0.9351.00
329_S371_Q0.9301.00
267_D270_T0.9281.00
146_I149_A0.9251.00
177_A180_S0.9241.00
195_T200_T0.9241.00
127_R143_D0.9221.00
117_A155_A0.9221.00
250_G255_L0.9201.00
128_Y141_E0.9131.00
318_G335_P0.9061.00
194_V202_L0.9031.00
50_E300_V0.9001.00
72_S195_T0.8891.00
62_Y212_Y0.8841.00
80_F203_A0.8821.00
323_L368_P0.8821.00
114_S159_A0.8781.00
103_N169_N0.8751.00
85_D284_H0.8731.00
259_L275_L0.8711.00
339_S346_W0.8711.00
110_E162_T0.8701.00
18_A22_A0.8691.00
326_N329_S0.8661.00
333_A368_P0.8641.00
93_Y186_S0.8641.00
85_D180_S0.8621.00
114_S117_A0.8621.00
307_I322_V0.8601.00
325_V329_S0.8581.00
51_V224_R0.8581.00
52_K297_D0.8571.00
48_P300_V0.8541.00
84_S90_Q0.8531.00
123_L126_K0.8511.00
24_C27_K0.8461.00
301_Q304_A0.8451.00
246_V252_T0.8381.00
114_S152_A0.8371.00
187_T204_T0.8361.00
107_A159_A0.8351.00
93_Y202_L0.8351.00
80_F186_S0.8341.00
71_V100_Y0.8291.00
181_G205_V0.8251.00
297_D300_V0.8251.00
325_V331_V0.8211.00
145_A148_D0.8211.00
19_A23_G0.8211.00
46_T305_I0.8181.00
117_A151_Q0.8161.00
359_I366_A0.8141.00
61_A288_P0.8131.00
187_T201_E0.8111.00
45_K354_S0.8021.00
112_A157_I0.8011.00
155_A159_A0.8011.00
217_Q275_L0.8001.00
94_Q97_P0.8001.00
97_P174_K0.7991.00
19_A24_C0.7991.00
179_I205_V0.7981.00
168_I171_A0.7971.00
80_F183_I0.7931.00
334_R353_K0.7921.00
108_K163_V0.7921.00
82_E186_S0.7911.00
76_L97_P0.7881.00
62_Y278_V0.7881.00
105_D164_E0.7871.00
250_G253_Y0.7861.00
323_L333_A0.7851.00
313_S321_T0.7851.00
117_A121_A0.7811.00
117_A148_D0.7791.00
168_I172_Y0.7771.00
315_T321_T0.7761.00
42_H308_P0.7751.00
226_K241_S0.7751.00
85_D208_L0.7731.00
211_I288_P0.7731.00
227_Q241_S0.7701.00
148_D151_Q0.7661.00
49_L342_I0.7661.00
69_P194_V0.7661.00
120_I147_A0.7631.00
331_V372_V0.7631.00
227_Q262_S0.7621.00
103_N165_S0.7611.00
166_A169_N0.7571.00
78_R93_Y0.7571.00
326_N356_D0.7551.00
331_V366_A0.7541.00
245_L257_G0.7521.00
124_T140_Q0.7511.00
217_Q225_L0.7501.00
98_A168_I0.7471.00
42_H45_K0.7471.00
111_L160_K0.7461.00
323_L331_V0.7461.00
348_I352_L0.7451.00
57_G292_V0.7441.00
67_V178_P0.7441.00
69_P93_Y0.7421.00
120_I123_L0.7391.00
215_V296_I0.7361.00
63_R190_E0.7361.00
322_V358_V0.7351.00
367_R370_E0.7341.00
321_T335_P0.7341.00
255_L283_Q0.7341.00
161_A164_E0.7321.00
63_R189_T0.7301.00
242_N297_D0.7271.00
74_I193_L0.7261.00
109_G161_A0.7241.00
123_L143_D0.7211.00
325_V359_I0.7191.00
37_P370_E0.7181.00
60_N212_Y0.7151.00
248_E293_R0.7151.00
53_T221_D0.7121.00
245_L294_A0.7101.00
182_R208_L0.7041.00
339_S351_G0.7041.00
152_A155_A0.7001.00
147_A151_Q0.6971.00
334_R351_G0.6951.00
75_V202_L0.6941.00
39_V359_I0.6921.00
120_I144_Q0.6921.00
159_A162_T0.6911.00
310_Q361_S0.6901.00
262_S275_L0.6901.00
51_V299_G0.6871.00
104_Y170_L0.6831.00
124_T148_D0.6821.00
151_Q155_A0.6781.00
70_Q99_T0.6781.00
62_Y207_Q0.6761.00
323_L366_A0.6741.00
55_L215_V0.6741.00
166_A170_L0.6731.00
115_E118_A0.6721.00
22_A26_D0.6721.00
357_Q374_A0.6711.00
64_I206_Q0.6701.00
152_A156_V0.6671.00
338_A348_I0.6641.00
307_I311_G0.6631.00
12_S19_A0.6621.00
22_A25_N0.6611.00
162_T165_S0.6601.00
44_V352_L0.6581.00
72_S193_L0.6551.00
247_M286_L0.6551.00
308_P311_G0.6541.00
53_T298_E0.6501.00
103_N107_A0.6501.00
20_L23_G0.6491.00
132_V137_I0.6441.00
44_V356_D0.6441.00
138_S141_E0.6431.00
107_A110_E0.6431.00
158_A162_T0.6411.00
63_R179_I0.6391.00
329_S374_A0.6331.00
117_A152_A0.6301.00
41_V360_V0.6291.00
139_Q146_I0.6281.00
107_A111_L0.6281.00
311_G360_V0.6271.00
17_S21_I0.6271.00
110_E114_S0.6261.00
327_D357_Q0.6241.00
253_Y283_Q0.6241.00
76_L79_N0.6231.00
141_E145_A0.6231.00
42_H345_K0.6221.00
55_L221_D0.6211.00
17_S20_L0.6201.00
36_E373_K0.6201.00
102_A168_I0.6181.00
249_N252_T0.6181.00
45_K345_K0.6171.00
345_K349_S0.6171.00
212_Y260_Q0.6161.00
61_A64_I0.6161.00
13_F16_I0.6151.00
188_V192_A0.6151.00
60_N64_I0.6141.00
132_V135_K0.6081.00
80_F84_S0.6081.00
155_A158_A0.6071.00
86_V92_L0.6071.00
42_H307_I0.6061.00
249_N253_Y0.6051.00
67_V203_A0.6051.00
55_L217_Q0.6031.00
260_Q276_R0.6011.00
65_A178_P0.5991.00
127_R131_L0.5981.00
287_L290_M0.5971.00
305_I351_G0.5961.00
137_I141_E0.5961.00
65_A205_V0.5961.00
273_I290_M0.5941.00
162_T166_A0.5901.00
55_L296_I0.5891.00
209_D284_H0.5891.00
57_G213_V0.5881.00
99_T169_N0.5871.00
66_E192_A0.5861.00
213_V279_F0.5861.00
49_L340_Q0.5841.00
51_V340_Q0.5831.00
171_A176_T0.5821.00
36_E371_Q0.5801.00
97_P170_L0.5801.00
329_S357_Q0.5781.00
307_I312_V0.5771.00
16_I19_A0.5741.00
103_N166_A0.5731.00
123_L147_A0.5711.00
51_V298_E0.5671.00
327_D330_Q0.5661.00
118_A125_V0.5651.00
13_F17_S0.5651.00
209_D282_P0.5631.00
49_L347_L0.5621.00
322_V351_G0.5591.00
55_L294_A0.5591.00
61_A206_Q0.5591.00
324_I358_V0.5591.00
116_A154_A0.5581.00
18_A24_C0.5581.00
13_F20_L0.5561.00
312_V336_V0.5561.00
219_S291_F0.5561.00
277_A294_A0.5551.00
100_Y166_A0.5551.00
137_I142_Y0.5541.00
68_R201_E0.5541.00
58_R289_G0.5531.00
366_A372_V0.5521.00
245_L277_A0.5501.00
49_L306_L0.5501.00
11_P18_A0.5491.00
352_L356_D0.5491.00
313_S323_L0.5481.00
109_G154_A0.5471.00
255_L286_L0.5471.00
69_P202_L0.5451.00
311_G358_V0.5441.00
211_I281_N0.5411.00
147_A150_R0.5411.00
99_T103_N0.5391.00
206_Q288_P0.5371.00
89_G176_T0.5371.00
128_Y132_V0.5371.00
35_G38_Q0.5361.00
17_S24_C0.5311.00
114_S118_A0.5301.00
42_H46_T0.5301.00
116_A120_I0.5301.00
124_T145_A0.5291.00
243_V296_I0.5271.00
46_T353_K0.5271.00
43_I46_T0.5271.00
107_A166_A0.5241.00
306_L347_L0.5231.00
79_N90_Q0.5221.00
266_V271_G0.5211.00
131_L138_S0.5181.00
219_S249_N0.5161.00
211_I279_F0.5161.00
121_A124_T0.5161.00
189_T192_A0.5161.00
206_Q264_V0.5161.00
201_E204_T0.5151.00
215_V275_L0.5141.00
225_L259_L0.5141.00
19_A22_A0.5121.00
45_K306_L0.5111.00
23_G27_K0.5111.00
188_V194_V0.5111.00
312_V338_A0.5111.00
156_V159_A0.5111.00
121_A148_D0.5101.00
110_E158_A0.5091.00
88_A178_P0.5091.00
188_V202_L0.5081.00
227_Q260_Q0.5071.00
78_R186_S0.5061.00
243_V247_M0.5051.00
102_A161_A0.5041.00
196_N200_T0.5041.00
256_K280_P0.5031.00
67_V179_I0.5031.00
59_T247_M0.5001.00
140_Q143_D0.5001.00
170_L173_T0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1vf7A 5 0.6234 100 0.241 Contact Map
3lnnA 6 0.8545 100 0.257 Contact Map
4dk0A 5 0.7896 100 0.261 Contact Map
3ne5B 3 0.8182 100 0.278 Contact Map
3fppA 3 0.6442 100 0.281 Contact Map
4l8jA 2 0.813 100 0.285 Contact Map
4kkuA 1 0.6831 100 0.333 Contact Map
2f1mA 2 0.6104 100 0.4 Contact Map
4tkoB 1 0.6052 100 0.483 Contact Map
2k32A 1 0.2987 99.8 0.707 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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