GREMLIN Database
HYAF - Hydrogenase-1 operon protein HyaF
UniProt: P19932 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10473
Length: 285 (256)
Sequences: 174 (145)
Seq/√Len: 9.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
174_L251_I7.8551.00
234_H247_E5.2121.00
79_N82_D5.1881.00
114_F275_V4.4100.99
224_I258_V4.2650.99
262_P266_V3.3730.95
22_M25_L2.8760.89
164_L167_G2.4310.78
57_Q232_V2.3920.77
97_V142_L2.3810.77
115_C251_I2.2410.72
117_L133_L2.1210.68
254_I259_L2.0430.65
164_L240_T2.0220.64
214_I258_V1.9800.62
240_T245_L1.8780.58
220_G239_D1.8500.57
188_I251_I1.8470.57
215_R221_E1.8360.56
80_A228_G1.8350.56
166_N220_G1.8330.56
204_S234_H1.8110.55
119_R131_D1.7590.53
23_N109_I1.7420.52
135_A143_W1.7390.52
99_I109_I1.7300.52
226_A237_C1.7160.51
114_F256_E1.7130.51
242_K245_L1.7070.51
121_R174_L1.6870.50
185_P188_I1.6520.48
87_N119_R1.6180.47
74_D197_E1.6090.46
120_V141_A1.6030.46
163_G198_A1.5890.45
188_I224_I1.5680.44
63_R137_C1.5680.44
165_M258_V1.5480.44
82_D133_L1.5370.43
60_L92_E1.5290.43
108_E121_R1.5030.42
73_V277_Q1.4930.41
260_A264_D1.4910.41
79_N196_S1.4730.40
82_D213_Q1.4700.40
221_E242_K1.4550.40
88_T276_C1.4540.40
83_R120_V1.4530.40
217_I223_Y1.4460.39
187_S248_S1.4440.39
191_T249_Y1.4260.38
189_N220_G1.4220.38
239_D245_L1.4210.38
221_E245_L1.4130.38
46_S82_D1.4040.38
234_H275_V1.4020.37
19_S23_N1.3790.37
107_S141_A1.3640.36
243_G249_Y1.3540.36
136_G185_P1.3490.35
193_L220_G1.3480.35
90_L133_L1.3470.35
31_V251_I1.3260.34
221_E257_V1.3230.34
24_P152_P1.3210.34
138_A142_L1.3170.34
25_L92_E1.3130.34
138_A258_V1.3080.34
44_A51_A1.3060.34
29_C240_T1.3010.33
216_T222_S1.2930.33
240_T244_P1.2900.33
110_Q261_A1.2840.33
29_C215_R1.2740.32
54_N136_G1.2620.32
49_V86_I1.2570.32
240_T257_V1.2510.32
108_E279_L1.2340.31
160_P272_L1.2120.30
192_Q222_S1.2050.30
39_A263_E1.2040.30
111_E264_D1.2000.30
39_A88_T1.1980.30
216_T235_L1.1920.29
145_A191_T1.1870.29
24_P132_K1.1830.29
125_G129_L1.1810.29
216_T223_Y1.1810.29
33_D136_G1.1770.29
113_I176_A1.1760.29
195_I258_V1.1760.29
68_G238_T1.1750.29
177_H247_E1.1720.29
77_N85_F1.1710.29
192_Q242_K1.1700.29
86_I90_L1.1690.28
140_L226_A1.1680.28
110_Q221_E1.1650.28
234_H278_W1.1580.28
29_C32_N1.1570.28
82_D252_C1.1530.28
178_V231_H1.1520.28
72_A132_K1.1500.28
23_N32_N1.1500.28
196_S229_L1.1490.28
267_D274_E1.1420.28
171_A226_A1.1400.27
234_H256_E1.1380.27
56_L90_L1.1360.27
240_T250_E1.1340.27
39_A221_E1.1330.27
256_E259_L1.1300.27
57_Q224_I1.1110.26
18_D136_G1.1090.26
80_A214_I1.0990.26
57_Q73_V1.0960.26
220_G261_A1.0910.26
178_V272_L1.0810.25
113_I274_E1.0790.25
138_A249_Y1.0620.25
76_L86_I1.0600.25
223_Y235_L1.0600.25
61_S154_D1.0540.25
86_I204_S1.0510.24
123_R237_C1.0500.24
78_L191_T1.0480.24
139_P218_G1.0460.24
128_L135_A1.0450.24
23_N113_I1.0420.24
186_H244_P1.0390.24
225_N236_R1.0380.24
111_E260_A1.0310.24
40_L88_T1.0290.24
192_Q216_T1.0270.24
41_E82_D1.0270.24
250_E257_V1.0210.24
62_E183_A1.0130.23
222_S242_K1.0130.23
177_H265_L1.0110.23
142_L278_W0.9990.23
50_I251_I0.9980.23
70_V136_G0.9950.23
110_Q220_G0.9940.23
176_A230_R0.9920.23
109_I144_Q0.9900.23
188_I228_G0.9840.22
159_P185_P0.9830.22
246_L260_A0.9730.22
61_S169_P0.9710.22
152_P159_P0.9600.22
246_L271_R0.9540.21
20_F23_N0.9540.21
217_I248_S0.9440.21
170_L203_L0.9420.21
166_N193_L0.9350.21
212_I234_H0.9290.21
22_M242_K0.9280.21
97_V121_R0.9270.21
22_M28_T0.9230.21
193_L219_Y0.9140.20
18_D86_I0.9140.20
87_N196_S0.9120.20
129_L188_I0.9120.20
221_E240_T0.9100.20
56_L275_V0.9080.20
99_I234_H0.8910.20
98_R278_W0.8900.20
236_R249_Y0.8870.20
110_Q242_K0.8870.20
77_N120_V0.8810.19
89_L197_E0.8810.19
43_C51_A0.8740.19
74_D105_S0.8690.19
80_A169_P0.8670.19
168_L190_L0.8650.19
164_L168_L0.8600.19
164_L191_T0.8560.19
170_L249_Y0.8550.19
215_R245_L0.8550.19
96_S119_R0.8510.19
41_E89_L0.8510.19
30_Q279_L0.8470.18
52_L170_L0.8460.18
170_L195_I0.8450.18
32_N213_Q0.8440.18
59_Q266_V0.8440.18
188_I249_Y0.8390.18
145_A165_M0.8370.18
91_G267_D0.8350.18
223_Y242_K0.8340.18
174_L188_I0.8340.18
97_V214_I0.8340.18
115_C236_R0.8310.18
195_I200_R0.8230.18
203_L277_Q0.8210.18
109_I274_E0.8190.18
245_L261_A0.8170.18
216_T258_V0.8130.18
112_A196_S0.8040.17
20_F221_E0.7970.17
57_Q247_E0.7970.17
86_I231_H0.7950.17
55_E59_Q0.7930.17
32_N157_L0.7920.17
200_R234_H0.7920.17
33_D112_A0.7910.17
222_S237_C0.7890.17
55_E129_L0.7860.17
136_G269_A0.7850.17
222_S235_L0.7800.17
114_F234_H0.7770.17
23_N61_S0.7760.17
120_V176_A0.7750.17
56_L88_T0.7730.17
33_D269_A0.7720.17
189_N192_Q0.7700.17
142_L269_A0.7670.16
51_A84_H0.7660.16
146_A268_S0.7640.16
110_Q245_L0.7610.16
83_R87_N0.7600.16
71_L188_I0.7590.16
115_C217_I0.7590.16
147_T195_I0.7590.16
130_E196_S0.7570.16
74_D109_I0.7550.16
51_A71_L0.7550.16
56_L204_S0.7550.16
237_C257_V0.7540.16
166_N239_D0.7540.16
63_R265_L0.7510.16
17_D33_D0.7480.16
92_E272_L0.7420.16
23_N265_L0.7420.16
55_E159_P0.7400.16
223_Y260_A0.7370.16
163_G174_L0.7280.16
17_D47_P0.7270.16
241_L247_E0.7240.15
89_L113_I0.7220.15
164_L249_Y0.7210.15
20_F115_C0.7160.15
46_S170_L0.7150.15
56_L133_L0.7120.15
43_C143_W0.7090.15
55_E81_D0.7070.15
192_Q235_L0.7060.15
59_Q73_V0.7040.15
250_E271_R0.7040.15
105_S279_L0.7030.15
46_S79_N0.7020.15
155_S201_L0.7010.15
166_N264_D0.7000.15
87_N96_S0.6910.15
27_I143_W0.6900.15
170_L278_W0.6880.15
68_G165_M0.6760.14
112_A275_V0.6750.14
80_A211_N0.6740.14
93_G139_P0.6730.14
65_P262_P0.6710.14
52_L259_L0.6700.14
190_L203_L0.6690.14
156_L253_P0.6560.14
222_S245_L0.6560.14
265_L273_S0.6530.14
164_L242_K0.6520.14
94_E111_E0.6510.14
92_E270_Q0.6510.14
35_P87_N0.6490.14
215_R222_S0.6470.14
58_H120_V0.6430.14
56_L123_R0.6430.14
117_L198_A0.6410.14
190_L247_E0.6400.14
83_R259_L0.6400.14
50_I253_P0.6360.14
225_N232_V0.6350.14
86_I237_C0.6330.14
166_N257_V0.6310.13
89_L129_L0.6310.13
60_L270_Q0.6310.13
188_I209_P0.6300.13
144_Q179_R0.6290.13
217_I251_I0.6250.13
219_Y250_E0.6250.13
178_V229_L0.6250.13
49_V231_H0.6240.13
22_M29_C0.6230.13
77_N231_H0.6220.13
86_I185_P0.6220.13
94_E239_D0.6220.13
69_E183_A0.6200.13
166_N237_C0.6190.13
114_F254_I0.6150.13
171_A195_I0.6120.13
192_Q220_G0.6080.13
139_P210_G0.6080.13
17_D254_I0.6060.13
183_A187_S0.6040.13
21_S24_P0.6030.13
165_M231_H0.6020.13
170_L236_R0.6020.13
211_N265_L0.6010.13
33_D163_G0.5970.13
61_S273_S0.5960.13
90_L98_R0.5930.13
118_W233_W0.5920.13
233_W255_P0.5920.13
194_P233_W0.5920.13
118_W194_P0.5920.13
118_W255_P0.5920.13
194_P255_P0.5920.13
128_L179_R0.5920.13
58_H154_D0.5910.13
220_G245_L0.5910.13
169_P277_Q0.5900.13
213_Q269_A0.5890.13
215_R242_K0.5830.13
89_L152_P0.5820.12
124_R128_L0.5810.12
78_L193_L0.5810.12
142_L211_N0.5800.12
237_C241_L0.5790.12
48_Q170_L0.5790.12
166_N242_K0.5770.12
19_S44_A0.5760.12
40_L46_S0.5760.12
120_V152_P0.5730.12
52_L107_S0.5730.12
52_L69_E0.5700.12
142_L275_V0.5700.12
77_N84_H0.5630.12
84_H88_T0.5620.12
82_D88_T0.5610.12
146_A210_G0.5590.12
52_L143_W0.5580.12
79_N277_Q0.5560.12
140_L144_Q0.5540.12
131_D172_H0.5520.12
166_N219_Y0.5500.12
70_V128_L0.5500.12
140_L181_P0.5500.12
27_I213_Q0.5490.12
200_R244_P0.5490.12
112_A243_G0.5470.12
19_S184_Q0.5460.12
67_L71_L0.5460.12
92_E133_L0.5450.12
148_Q188_I0.5430.12
44_A181_P0.5420.12
49_V257_V0.5380.12
24_P90_L0.5340.12
86_I138_A0.5340.12
166_N260_A0.5330.12
215_R240_T0.5330.12
216_T237_C0.5310.12
115_C198_A0.5300.12
246_L267_D0.5290.12
170_L197_E0.5270.11
60_L185_P0.5260.11
95_V267_D0.5260.11
43_C114_F0.5230.11
222_S260_A0.5210.11
79_N112_A0.5190.11
180_N183_A0.5180.11
82_D117_L0.5180.11
223_Y245_L0.5180.11
220_G260_A0.5170.11
192_Q219_Y0.5140.11
166_N245_L0.5130.11
200_R278_W0.5130.11
220_G264_D0.5120.11
219_Y264_D0.5100.11
199_D263_E0.5100.11
236_R247_E0.5070.11
114_F137_C0.5040.11
196_S276_C0.5040.11
49_V191_T0.5030.11
147_T244_P0.5020.11
198_A229_L0.5020.11
152_P185_P0.5010.11
44_A148_Q0.5000.11
235_L248_S0.5000.11
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3sb1A 2 0.414 100 0.632 Contact Map
2ka6B 1 0.1439 49.5 0.967 Contact Map
2wulA 3 0.2982 16.2 0.974 Contact Map
3gx8A 1 0.3088 15.4 0.975 Contact Map
4wxtA 1 0.3298 10.8 0.976 Contact Map
3zywA 1 0.3088 10.1 0.977 Contact Map
2i0kA 1 0.4175 8.7 0.977 Contact Map
1q7lB 2 0.193 8.7 0.977 Contact Map
3js8A 1 0.414 8.5 0.977 Contact Map
1iloA 1 0.2491 8.3 0.977 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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