NARW - ECOLI - GREMLIN Contacts

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NARW - Probable nitrate reductase molybdenum cofactor assembly chaperone NarW

UniProt:	P19317	Sequences: MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLLNAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRELPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGSSLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDVAPQYVDISAGGGK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG10645
Length:	231 (183)
Sequences:	669 (494)
Seq/√Len:	36.5

ECOLI - NARW NARJ
BACSU - NARJ

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
39_F	150_L	4.689	1.00
26_L	44_L	3.951	1.00
106_Y	109_L	3.853	1.00
48_L	52_Q	3.597	1.00
6_V	25_A	3.253	1.00
26_L	41_H	2.937	1.00
89_A	93_K	2.796	1.00
38_D	149_A	2.689	1.00
7_I	154_L	2.558	1.00
42_N	146_P	2.543	1.00
137_G	155_L	2.515	1.00
141_K	152_D	2.398	1.00
23_E	44_L	2.391	1.00
7_I	150_L	2.318	0.99
39_F	149_A	2.310	0.99
4_L	157_L	2.227	0.99
56_C	60_D	2.189	0.99
18_L	43_L	2.188	0.99
92_E	97_Q	2.146	0.99
134_A	137_G	2.132	0.99
42_N	54_E	2.117	0.99
21_C	24_D	2.113	0.99
89_A	92_E	1.872	0.98
110_Y	125_G	1.857	0.98
58_V	145_A	1.856	0.97
39_F	43_L	1.836	0.97
22_K	40_T	1.833	0.97
16_E	52_Q	1.807	0.97
114_L	122_A	1.730	0.96
135_L	172_N	1.720	0.96
36_L	150_L	1.672	0.95
23_E	27_A	1.598	0.94
65_T	139_R	1.583	0.94
87_L	90_E	1.567	0.93
61_R	71_E	1.555	0.93
74_H	83_A	1.548	0.93
98_L	109_L	1.519	0.92
29_I	32_D	1.512	0.92
124_E	128_N	1.487	0.91
22_K	44_L	1.484	0.91
4_L	114_L	1.483	0.91
6_V	53_A	1.474	0.91
90_E	125_G	1.456	0.91
5_K	8_G	1.455	0.91
62_G	143_R	1.446	0.90
9_L	21_C	1.432	0.90
47_P	50_D	1.423	0.90
136_L	151_F	1.422	0.89
170_Q	174_E	1.383	0.88
87_L	118_P	1.366	0.87
16_E	20_E	1.365	0.87
70_F	87_L	1.330	0.86
55_W	75_A	1.326	0.86
163_S	168_T	1.300	0.85
35_M	38_D	1.299	0.85
49_L	53_A	1.261	0.83
65_T	136_L	1.239	0.82
46_A	54_E	1.238	0.82
63_R	71_E	1.238	0.82
74_H	87_L	1.230	0.81
43_L	51_K	1.228	0.81
26_L	37_T	1.210	0.80
73_V	132_I	1.199	0.80
4_L	117_L	1.188	0.79
88_L	98_L	1.172	0.78
34_P	38_D	1.158	0.77
74_H	79_D	1.141	0.76
76_E	182_A	1.138	0.76
88_L	92_E	1.134	0.76
29_I	33_A	1.126	0.75
175_E	181_Q	1.086	0.72
79_D	84_M	1.084	0.72
3_I	32_D	1.077	0.72
135_L	179_T	1.074	0.72
91_Y	113_Y	1.070	0.71
72_H	132_I	1.058	0.70
7_I	153_A	1.055	0.70
84_M	103_L	1.046	0.69
30_R	37_T	1.043	0.69
67_L	108_P	1.036	0.69
54_E	147_W	1.035	0.69
94_V	116_V	1.029	0.68
38_D	118_P	1.026	0.68
49_L	84_M	1.015	0.67
12_E	106_Y	1.013	0.67
139_R	178_D	1.008	0.67
20_E	23_E	0.997	0.66
117_L	121_Q	0.995	0.66
34_P	164_S	0.987	0.65
72_H	147_W	0.964	0.63
25_A	28_L	0.956	0.62
160_S	163_S	0.953	0.62
123_K	127_L	0.945	0.61
5_K	24_D	0.942	0.61
10_L	70_F	0.940	0.61
21_C	25_A	0.932	0.60
67_L	135_L	0.929	0.60
43_L	60_D	0.929	0.60
88_L	137_G	0.920	0.59
98_L	113_Y	0.920	0.59
94_V	113_Y	0.911	0.59
131_P	135_L	0.910	0.59
10_L	72_H	0.908	0.58
4_L	96_L	0.899	0.58
73_V	129_V	0.895	0.57
130_A	176_R	0.893	0.57
49_L	65_T	0.889	0.57
40_T	46_A	0.886	0.57
141_K	148_Y	0.871	0.55
65_T	72_H	0.869	0.55
5_K	91_Y	0.864	0.55
27_A	121_Q	0.859	0.54
28_L	31_R	0.842	0.53
96_L	117_L	0.838	0.52
39_F	54_E	0.836	0.52
127_L	135_L	0.830	0.52
10_L	63_R	0.817	0.50
175_E	179_T	0.815	0.50
52_Q	143_R	0.813	0.50
67_L	103_L	0.809	0.50
8_G	21_C	0.809	0.50
51_K	150_L	0.806	0.50
93_K	168_T	0.797	0.49
6_V	29_I	0.797	0.49
82_Q	129_V	0.793	0.48
85_V	184_D	0.789	0.48
130_A	167_V	0.783	0.48
97_Q	181_Q	0.783	0.48
66_S	132_I	0.775	0.47
27_A	31_R	0.772	0.47
18_L	34_P	0.772	0.47
6_V	128_N	0.767	0.46
5_K	96_L	0.767	0.46
66_S	92_E	0.766	0.46
28_L	60_D	0.765	0.46
8_G	19_W	0.765	0.46
68_L	94_V	0.764	0.46
155_L	161_S	0.763	0.46
165_D	168_T	0.763	0.46
76_E	139_R	0.758	0.45
35_M	149_A	0.757	0.45
71_E	145_A	0.754	0.45
53_A	57_E	0.754	0.45
63_R	83_A	0.751	0.45
15_D	144_E	0.749	0.45
6_V	153_A	0.748	0.44
142_Q	166_S	0.741	0.44
37_T	40_T	0.739	0.44
130_A	158_A	0.738	0.44
169_K	173_S	0.738	0.44
62_G	65_T	0.736	0.43
93_K	118_P	0.735	0.43
17_L	20_E	0.733	0.43
78_R	107_L	0.729	0.43
46_A	51_K	0.717	0.42
29_I	115_S	0.713	0.41
82_Q	86_D	0.711	0.41
38_D	101_R	0.709	0.41
25_A	29_I	0.704	0.41
151_F	178_D	0.700	0.40
20_E	120_D	0.700	0.40
22_K	150_L	0.699	0.40
165_D	184_D	0.699	0.40
37_T	43_L	0.698	0.40
31_R	50_D	0.696	0.40
41_H	141_K	0.695	0.40
90_E	110_Y	0.695	0.40
176_R	179_T	0.695	0.40
71_E	130_A	0.690	0.40
91_Y	111_L	0.689	0.39
147_W	161_S	0.683	0.39
153_A	159_G	0.679	0.39
177_D	180_R	0.679	0.39
74_H	91_Y	0.676	0.38
131_P	138_G	0.672	0.38
86_D	143_R	0.666	0.38
71_E	110_Y	0.663	0.37
181_Q	185_A	0.663	0.37
87_L	110_Y	0.662	0.37
5_K	116_V	0.661	0.37
135_L	177_D	0.661	0.37
107_L	171_V	0.658	0.37
91_Y	154_L	0.655	0.37
148_Y	154_L	0.652	0.36
137_G	151_F	0.651	0.36
43_L	118_P	0.650	0.36
3_I	37_T	0.648	0.36
60_D	100_C	0.647	0.36
137_G	160_S	0.642	0.36
21_C	53_A	0.637	0.35
72_H	87_L	0.637	0.35
96_L	116_V	0.634	0.35
17_L	21_C	0.633	0.35
101_R	124_E	0.632	0.35
82_Q	85_V	0.631	0.35
57_E	160_S	0.626	0.34
132_I	182_A	0.621	0.34
71_E	76_E	0.618	0.34
49_L	96_L	0.614	0.33
4_L	70_F	0.612	0.33
66_S	82_Q	0.612	0.33
138_G	142_Q	0.612	0.33
22_K	27_A	0.608	0.33
83_A	103_L	0.607	0.33
7_I	157_L	0.606	0.33
82_Q	180_R	0.606	0.33
12_E	154_L	0.604	0.33
55_W	60_D	0.602	0.32
36_L	40_T	0.601	0.32
79_D	155_L	0.597	0.32
19_W	23_E	0.596	0.32
106_Y	151_F	0.596	0.32
131_P	163_S	0.595	0.32
41_H	54_E	0.594	0.32
37_T	45_N	0.586	0.31
97_Q	174_E	0.585	0.31
45_N	54_E	0.584	0.31
67_L	154_L	0.583	0.31
5_K	28_L	0.582	0.31
83_A	98_L	0.581	0.31
15_D	119_D	0.581	0.31
64_T	176_R	0.581	0.31
74_H	184_D	0.576	0.30
45_N	50_D	0.576	0.30
17_L	146_P	0.575	0.30
57_E	62_G	0.574	0.30
9_L	171_V	0.574	0.30
43_L	150_L	0.574	0.30
84_M	104_P	0.569	0.30
129_V	177_D	0.569	0.30
30_R	34_P	0.565	0.30
5_K	9_L	0.563	0.30
5_K	157_L	0.562	0.29
130_A	181_Q	0.560	0.29
11_M	129_V	0.559	0.29
68_L	108_P	0.559	0.29
27_A	35_M	0.557	0.29
97_Q	115_S	0.557	0.29
62_G	177_D	0.556	0.29
61_R	76_E	0.554	0.29
70_F	86_D	0.554	0.29
112_E	148_Y	0.553	0.29
143_R	184_D	0.553	0.29
94_V	118_P	0.550	0.29
8_G	123_K	0.550	0.29
122_A	166_S	0.548	0.28
94_V	106_Y	0.548	0.28
32_D	153_A	0.545	0.28
4_L	9_L	0.544	0.28
15_D	181_Q	0.543	0.28
104_P	136_L	0.543	0.28
110_Y	129_V	0.541	0.28
52_Q	180_R	0.540	0.28
55_W	147_W	0.536	0.28
15_D	19_W	0.532	0.27
6_V	123_K	0.529	0.27
179_T	182_A	0.527	0.27
64_T	181_Q	0.527	0.27
48_L	66_S	0.527	0.27
77_S	182_A	0.526	0.27
10_L	83_A	0.525	0.27
56_C	75_A	0.524	0.27
66_S	74_H	0.521	0.27
62_G	122_A	0.517	0.26
34_P	41_H	0.516	0.26
35_M	108_P	0.511	0.26
94_V	183_L	0.511	0.26
61_R	131_P	0.506	0.26
119_D	136_L	0.506	0.26
174_E	178_D	0.505	0.25
56_C	110_Y	0.501	0.25
147_W	162_L	0.501	0.25
26_L	30_R	0.500	0.25
34_P	120_D	0.500	0.25

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2o9xA	2	0.645	99.9	0.686	Contact Map
1n1cA	2	0.7446	99.9	0.722	Contact Map
1s9uA	1	0.7446	99.8	0.742	Contact Map
2xolA	2	0.6797	97.6	0.898	Contact Map
3viqB	1	0.329	29.6	0.96	Contact Map
3b02A	2	0.3247	13.8	0.965	Contact Map
2yskA	2	0.5801	13.4	0.966	Contact Map
1rj9A	1	0.4892	8	0.969	Contact Map
2fa5A	2	0.4459	6.6	0.97	Contact Map
2kl4A	1	0.4935	6.6	0.97	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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