GREMLIN Database
NADE - NH(3)-dependent NAD(+) synthetase
UniProt: P18843 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10663
Length: 275 (238)
Sequences: 4560 (2560)
Seq/√Len: 166.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
203_E207_K4.8661.00
81_V149_I3.7341.00
60_C158_V3.4401.00
42_S155_G3.4001.00
253_N257_K3.1191.00
23_I198_A2.7801.00
96_D204_H2.6701.00
88_Q92_Q2.5161.00
250_T254_W2.5101.00
245_Q249_R2.4551.00
80_A104_D2.4361.00
55_L196_L2.3541.00
34_L43_L2.2711.00
20_E198_A2.2131.00
30_L59_L2.1811.00
98_I106_V2.0671.00
112_K116_L2.0511.00
47_I98_I2.0221.00
93_D208_K1.9801.00
259_E263_R1.9741.00
46_G146_Q1.9361.00
95_Q99_A1.9331.00
118_S144_K1.9321.00
19_A194_Q1.8951.00
57_G104_D1.8821.00
56_A184_L1.8561.00
141_E144_K1.8061.00
233_N247_V1.7381.00
23_I62_M1.7031.00
113_G120_Q1.6761.00
51_Q196_L1.6571.00
96_D99_A1.6291.00
240_G243_V1.6261.00
43_L64_I1.6221.00
93_D204_H1.6141.00
118_S141_E1.6101.00
82_R106_V1.6091.00
197_A203_E1.5641.00
185_Y268_V1.5581.00
95_Q108_T1.5481.00
194_Q197_A1.5421.00
105_R153_T1.5341.00
173_K260_H1.5291.00
233_N244_P1.5251.00
42_S79_I1.5131.00
193_K232_D1.4861.00
28_D32_S1.4781.00
54_T205_L1.4661.00
137_E144_K1.4581.00
230_T233_N1.4491.00
171_F177_G1.4451.00
79_I107_L1.4411.00
222_P225_V1.4381.00
59_L183_P1.4291.00
188_N191_Q1.4261.00
79_I155_G1.3881.00
197_A206_Y1.3861.00
43_L60_C1.3791.00
61_Q66_E1.3741.00
133_V148_S1.3731.00
149_I153_T1.3721.00
20_E24_R1.3701.00
236_D241_K1.3671.00
168_T234_I1.3661.00
43_L158_V1.3611.00
224_E230_T1.3611.00
115_V141_E1.3501.00
42_S77_Q1.3361.00
163_A186_R1.3301.00
97_A205_L1.3271.00
96_D100_F1.3231.00
209_A222_P1.3141.00
24_R66_E1.3091.00
254_W257_K1.3041.00
38_P156_V1.3031.00
57_G101_I1.3011.00
41_K154_S1.2891.00
92_Q204_H1.2861.00
179_T182_N1.2791.00
238_L255_Y1.2591.00
168_T227_L1.2491.00
30_L43_L1.2481.00
51_Q192_G1.2461.00
89_A204_H1.2371.00
254_W258_T1.2351.00
95_Q106_V1.2351.00
91_E95_Q1.2241.00
114_A121_A1.2231.00
44_V149_I1.1931.00
190_R236_D1.1901.00
82_R91_E1.1891.00
58_K62_M1.1791.00
197_A207_K1.1791.00
33_Y36_T1.1781.00
250_T253_N1.1781.00
24_R28_D1.1741.00
188_N238_L1.1671.00
258_T262_R1.1641.00
115_V119_E1.1491.00
82_R94_C1.1451.00
34_L64_I1.1441.00
57_G80_A1.1381.00
23_I199_L1.1371.00
92_Q96_D1.1301.00
114_A141_E1.1191.00
62_M199_L1.1121.00
168_T254_W1.1121.00
114_A145_A1.1041.00
31_K35_Q1.0911.00
61_Q78_F1.0891.00
177_G265_P1.0821.00
65_N78_F1.0821.00
61_Q101_I1.0791.00
114_A118_S1.0741.00
236_D243_V1.0741.00
162_H223_D1.0731.00
91_E108_T1.0650.99
56_A158_V1.0610.99
249_R253_N1.0570.99
187_L191_Q1.0560.99
259_E262_R1.0510.99
34_L38_P1.0450.99
23_I27_V1.0400.99
215_E226_A1.0400.99
27_V31_K1.0350.99
23_I59_L1.0270.99
193_K209_A1.0100.99
158_V181_I1.0090.99
246_Q250_T1.0070.99
89_A208_K0.9970.99
107_L153_T0.9910.99
159_G179_T0.9880.99
31_K66_E0.9840.99
90_D213_D0.9830.99
212_A223_D0.9780.99
35_Q77_Q0.9760.99
116_L119_E0.9740.99
244_P247_V0.9650.99
77_Q105_R0.9650.99
181_I269_F0.9560.99
182_N185_Y0.9560.99
161_D171_F0.9530.99
80_A98_I0.9500.99
51_Q206_Y0.9450.99
174_Y178_G0.9440.99
111_I141_E0.9410.99
29_F269_F0.9400.99
27_V63_A0.9380.99
58_K101_I0.9380.99
95_Q98_I0.9360.99
86_G89_A0.9330.99
26_S59_L0.9300.99
114_A117_A0.9270.99
79_I105_R0.9260.99
210_P223_D0.9220.98
116_L120_Q0.9150.98
100_F201_C0.9140.98
32_S36_T0.9130.98
61_Q65_N0.9060.98
54_T97_A0.8910.98
87_V90_D0.8900.98
191_Q194_Q0.8880.98
134_R152_M0.8870.98
143_M178_G0.8850.98
82_R95_Q0.8840.98
117_A120_Q0.8790.98
122_L148_S0.8780.98
30_L181_I0.8770.98
92_Q95_Q0.8650.98
173_K180_D0.8630.98
117_A121_A0.8590.98
237_Y252_E0.8570.98
133_V152_M0.8520.97
109_V145_A0.8520.97
166_A171_F0.8450.97
180_D260_H0.8430.97
42_S153_T0.8420.97
97_A100_F0.8390.97
212_A218_R0.8380.97
83_L145_A0.8330.97
109_V121_A0.8280.97
22_E191_Q0.8260.97
113_G121_A0.8220.97
141_E145_A0.8210.97
196_L206_Y0.8120.97
237_Y248_A0.8070.96
170_F226_A0.8050.96
235_D238_L0.8040.96
170_F212_A0.8020.96
127_I152_M0.8020.96
248_A252_E0.8000.96
246_Q249_R0.8000.96
157_V178_G0.7970.96
30_L183_P0.7930.96
44_V155_G0.7880.96
45_L56_A0.7850.96
147_Y180_D0.7830.96
239_E242_N0.7790.96
136_N172_T0.7780.96
28_D31_K0.7730.96
113_G117_A0.7670.95
79_I153_T0.7670.95
62_M65_N0.7630.95
85_Y90_D0.7600.95
110_N113_G0.7590.95
157_V260_H0.7570.95
162_H210_P0.7530.95
92_Q99_A0.7520.95
64_I77_Q0.7490.95
137_E148_S0.7390.94
40_I154_S0.7390.94
80_A205_L0.7340.94
88_Q91_E0.7300.94
26_S186_R0.7280.94
64_I78_F0.7270.94
190_R239_E0.7260.94
45_L57_G0.7210.94
148_S152_M0.7200.93
27_V62_M0.7200.93
101_I104_D0.7170.93
82_R108_T0.7100.93
190_R235_D0.7090.93
185_Y269_F0.7040.93
22_E26_S0.6980.92
190_R232_D0.6980.92
30_L64_I0.6960.92
46_G83_L0.6960.92
136_N140_R0.6950.92
41_K77_Q0.6930.92
113_G116_L0.6930.92
192_G196_L0.6910.92
135_G226_A0.6910.92
243_V248_A0.6880.92
166_A265_P0.6880.92
174_Y179_T0.6880.92
159_G178_G0.6860.92
150_A260_H0.6850.92
252_E256_L0.6840.92
19_A198_A0.6780.91
109_V122_L0.6740.91
34_L42_S0.6690.91
247_V250_T0.6690.91
234_I251_I0.6610.90
55_L192_G0.6600.90
166_A258_T0.6590.90
22_E25_R0.6580.90
166_A185_Y0.6570.90
170_F218_R0.6560.90
86_G112_K0.6540.90
162_H212_A0.6520.90
209_A224_E0.6410.89
234_I237_Y0.6410.89
58_K97_A0.6410.89
133_V151_G0.6370.89
89_A93_D0.6340.88
31_K63_A0.6330.88
58_K100_F0.6310.88
61_Q104_D0.6260.88
167_I255_Y0.6260.88
138_K213_D0.6260.88
107_L149_I0.6250.88
58_K65_N0.6250.88
81_V107_L0.6240.87
137_E141_E0.6220.87
245_Q248_A0.6220.87
233_N236_D0.6200.87
96_D202_P0.6190.87
159_G183_P0.6160.87
65_N104_D0.6160.87
24_R27_V0.6160.87
83_L157_V0.6150.87
193_K206_Y0.6140.87
224_E228_G0.6090.86
107_L110_N0.6070.86
32_S35_Q0.6050.86
193_K197_A0.6050.86
256_L259_E0.6040.86
228_G231_Y0.6040.86
111_I115_V0.5960.85
164_A234_I0.5950.85
82_R86_G0.5950.85
233_N237_Y0.5920.85
43_L181_I0.5920.85
81_V145_A0.5910.85
91_E94_C0.5900.84
60_C78_F0.5900.84
66_E76_L0.5890.84
126_G129_L0.5870.84
89_A92_Q0.5860.84
135_G139_A0.5850.84
43_L156_V0.5850.84
194_Q198_A0.5840.84
55_L199_L0.5820.84
80_A106_V0.5810.84
97_A101_I0.5810.84
130_S152_M0.5800.83
163_A185_Y0.5800.83
47_I97_A0.5780.83
93_D96_D0.5770.83
18_N21_E0.5760.83
115_V137_E0.5720.83
83_L146_Q0.5710.82
48_S52_D0.5700.82
119_E137_E0.5690.82
58_K201_C0.5670.82
86_G90_D0.5670.82
166_A182_N0.5660.82
239_E248_A0.5650.82
107_L125_A0.5640.82
161_D182_N0.5620.81
168_T251_I0.5610.81
130_S133_V0.5610.81
135_G215_E0.5580.81
181_I185_Y0.5580.81
161_D177_G0.5560.81
105_R155_G0.5560.81
132_F135_G0.5550.81
222_P226_A0.5520.80
224_E232_D0.5500.80
66_E102_Q0.5490.80
21_E25_R0.5480.80
38_P185_Y0.5470.80
172_T261_K0.5460.80
242_N248_A0.5460.80
85_Y116_L0.5450.80
36_T259_E0.5440.79
131_D134_R0.5430.79
123_R251_I0.5390.79
89_A94_C0.5380.79
150_A155_G0.5380.79
161_D166_A0.5370.79
135_G138_K0.5350.78
51_Q189_K0.5330.78
98_I104_D0.5320.78
118_S148_S0.5310.78
232_D239_E0.5290.77
119_E123_R0.5290.77
51_Q193_K0.5290.77
173_K177_G0.5280.77
200_A206_Y0.5260.77
164_A231_Y0.5250.77
139_A214_L0.5230.77
48_S165_E0.5190.76
218_R222_P0.5170.76
161_D179_T0.5160.76
184_L192_G0.5150.76
42_S154_S0.5150.76
112_K115_V0.5150.76
128_E131_D0.5110.75
139_A172_T0.5100.75
243_V251_I0.5100.75
136_N139_A0.5090.75
243_V247_V0.5060.74
143_M147_Y0.5040.74
224_E236_D0.5010.74
178_G182_N0.5000.73
79_I149_I0.5000.73
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1wxiA 2 0.9455 100 0.328 Contact Map
1kqpA 2 0.9709 100 0.34 Contact Map
4q16A 2 0.9709 100 0.343 Contact Map
3q4gA 2 0.9273 100 0.35 Contact Map
4xfdA 1 0.9018 100 0.352 Contact Map
3ilvA 2 0.8364 100 0.362 Contact Map
3dpiA 3 0.9309 100 0.375 Contact Map
4f4hA 2 0.8873 100 0.392 Contact Map
3n05A 2 0.8691 100 0.399 Contact Map
3sdbA 6 0.88 100 0.41 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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