GREMLIN Database
PNCB - Nicotinate phosphoribosyltransferase
UniProt: P18133 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10742
Length: 400 (348)
Sequences: 2817 (1417)
Seq/√Len: 75.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
280_L294_A4.9081.00
207_A214_P4.8611.00
75_S82_A4.1011.00
228_Q239_A3.9711.00
68_D89_R3.6741.00
28_H81_K3.1871.00
57_Q125_L3.0501.00
95_P113_G2.8501.00
39_F125_L2.7801.00
25_A222_W2.7461.00
113_G118_V2.7221.00
67_Q70_E2.7011.00
261_T268_D2.6551.00
257_T294_A2.6151.00
200_G215_M2.5891.00
68_D72_Q2.5661.00
70_E208_R2.5231.00
261_T265_F2.4981.00
176_T185_Q2.4491.00
238_R242_A2.4431.00
205_D208_R2.4201.00
293_K296_A2.4181.00
257_T280_L2.3421.00
34_H112_S2.3121.00
61_M97_Q2.3011.00
254_I279_G2.2711.00
94_N117_E2.1781.00
296_A300_K2.1641.00
24_Q81_K2.1121.00
86_N89_R2.1121.00
61_M121_W2.0871.00
257_T297_H2.0581.00
39_F57_Q2.0451.00
48_G155_V2.0281.00
39_F129_I2.0151.00
265_F268_D2.0021.00
325_Y336_F1.9721.00
311_V335_S1.9081.00
270_G274_A1.9031.00
321_A338_I1.8991.00
320_K323_E1.8721.00
36_A112_S1.8571.00
257_T262_M1.8451.00
82_A86_N1.8431.00
158_S199_V1.8271.00
48_G159_A1.8031.00
158_S171_L1.7971.00
57_Q109_I1.7901.00
23_Q84_Y1.7811.00
242_A246_E1.7751.00
172_M215_M1.7671.00
263_D293_K1.7641.00
26_V119_I1.7601.00
71_Y85_L1.7591.00
87_W116_R1.7571.00
169_F336_F1.7541.00
291_G312_F1.7251.00
194_Q213_T1.7201.00
265_F269_F1.7071.00
269_F277_Y1.7041.00
17_A77_L1.7011.00
169_F325_Y1.6951.00
271_V276_R1.6881.00
114_P117_E1.6781.00
69_D208_R1.6731.00
34_H362_N1.6641.00
226_H243_A1.6621.00
158_S163_G1.6481.00
111_L356_I1.6401.00
295_I299_E1.6391.00
194_Q212_L1.6381.00
77_L214_P1.6361.00
189_V206_L1.6321.00
360_E363_G1.6011.00
205_D209_R1.6011.00
170_H199_V1.5900.99
29_H243_A1.5820.99
23_Q64_L1.5800.99
189_V343_T1.5560.99
24_Q28_H1.5500.99
51_A100_V1.5470.99
60_A124_P1.5430.99
109_I129_I1.5410.99
172_M200_G1.5300.99
41_C44_D1.5090.99
171_L198_F1.5010.99
309_T334_L1.4940.99
261_T264_A1.4930.99
80_F85_L1.4590.99
98_V112_S1.4530.99
270_G275_S1.4190.99
29_H246_E1.4160.99
288_V327_H1.4080.99
100_V384_V1.3960.99
112_S381_K1.3960.99
64_L91_F1.3850.99
324_L331_R1.3800.99
24_Q248_Y1.3760.98
111_L125_L1.3490.98
279_G308_K1.3390.98
215_M335_S1.3330.98
266_L297_H1.3210.98
266_L294_A1.3180.98
294_A297_H1.3170.98
35_V115_W1.3060.98
239_A243_A1.3000.98
95_P117_E1.2870.98
264_A267_R1.2780.98
30_Y33_V1.2770.98
317_D341_R1.2720.97
96_E112_S1.2710.97
66_L71_Y1.2650.97
262_M265_F1.2610.97
264_A268_D1.2490.97
322_V326_R1.2420.97
17_A214_P1.2420.97
17_A204_Y1.2250.97
282_H290_W1.2120.97
271_V303_I1.2120.97
176_T203_N1.2110.97
28_H247_E1.1960.96
75_S85_L1.1950.96
22_M219_A1.1940.96
182_R205_D1.1820.96
28_H83_D1.1760.96
243_A246_E1.1750.96
133_V137_R1.1680.96
157_F171_L1.1660.96
73_W214_P1.1550.96
65_R92_R1.1540.95
72_Q75_S1.1490.95
297_H301_L1.1480.95
33_V362_N1.1390.95
23_Q116_R1.1300.95
49_I135_R1.1300.95
26_V115_W1.1280.95
315_N353_N1.1270.95
115_W225_A1.1220.95
28_H246_E1.1120.94
312_F333_Q1.1110.94
57_Q124_P1.1050.94
51_A54_I1.1050.94
71_Y89_R1.0980.94
55_R59_Q1.0950.94
36_A360_E1.0950.94
218_Q223_F1.0950.94
340_T351_P1.0900.94
20_L74_L1.0820.94
263_D297_H1.0810.94
303_I308_K1.0800.94
86_N90_E1.0710.93
44_D49_I1.0680.93
282_H312_F1.0680.93
24_Q247_E1.0630.93
287_P333_Q1.0620.93
21_H80_F1.0620.93
14_D176_T1.0580.93
46_L345_D1.0530.93
72_Q76_A1.0480.93
295_I305_P1.0480.93
101_S104_N1.0430.92
75_S89_R1.0430.92
288_V324_L1.0400.92
262_M266_L1.0390.92
97_Q121_W1.0380.92
38_E382_A1.0360.92
73_W76_A1.0330.92
130_S354_I1.0280.92
91_F117_E1.0260.92
304_D308_K1.0210.92
98_V110_R1.0200.91
225_A361_C1.0170.91
49_I352_L1.0150.91
292_E327_H1.0080.91
156_D318_L1.0070.91
238_R267_R1.0050.91
154_L198_F1.0050.91
262_M268_D1.0020.91
193_Q211_S0.9930.90
275_S278_Q0.9860.90
279_G309_T0.9820.90
60_A64_L0.9800.90
94_N113_G0.9790.90
61_M124_P0.9760.90
262_M294_A0.9730.89
158_S162_A0.9720.89
257_T266_L0.9710.89
29_H247_E0.9660.89
38_E110_R0.9620.89
172_M335_S0.9620.89
341_R346_I0.9610.89
245_L268_D0.9560.89
261_T266_L0.9520.88
262_M297_H0.9510.88
56_E59_Q0.9410.88
272_E275_S0.9340.87
30_Y226_H0.9260.87
347_P350_K0.9250.87
295_I334_L0.9250.87
297_H300_K0.9200.87
250_D275_S0.9190.87
71_Y75_S0.9180.87
110_R381_K0.9140.86
175_G179_R0.9120.86
315_N340_T0.9010.85
126_L130_S0.8960.85
188_I191_R0.8960.85
345_D350_K0.8950.85
134_H192_L0.8920.85
254_I308_K0.8910.85
61_M95_P0.8890.85
206_L212_L0.8880.85
204_Y214_P0.8850.84
271_V305_P0.8780.84
59_Q63_H0.8770.84
171_L321_A0.8770.84
295_I332_V0.8760.84
280_L297_H0.8750.84
121_W125_L0.8630.83
304_D307_S0.8610.83
73_W77_L0.8570.82
389_K392_D0.8530.82
277_Y308_K0.8490.82
41_C49_I0.8480.82
276_R307_S0.8470.82
270_G273_F0.8430.81
30_Y225_A0.8370.81
249_P273_F0.8280.80
254_I311_V0.8250.80
57_Q129_I0.8240.80
227_Q366_V0.8180.79
288_V292_E0.8170.79
38_E359_V0.8160.79
34_H114_P0.8130.79
245_L253_G0.8130.79
124_P128_V0.8130.79
238_R268_D0.8090.79
41_C354_I0.8060.78
100_V104_N0.8050.78
35_V361_C0.8040.78
25_A247_E0.8010.78
24_Q85_L0.7980.78
86_N363_G0.7980.78
237_Q287_P0.7970.78
20_L80_F0.7950.78
15_T20_L0.7950.78
171_L199_V0.7940.77
74_L85_L0.7920.77
73_W207_A0.7900.77
25_A248_Y0.7880.77
128_V182_R0.7870.77
60_A121_W0.7850.77
123_V176_T0.7840.77
250_D276_R0.7840.77
33_V363_G0.7840.77
157_F338_I0.7830.76
193_Q342_L0.7830.76
57_Q121_W0.7810.76
53_A136_Y0.7810.76
76_A211_S0.7790.76
239_A242_A0.7790.76
175_G343_T0.7780.76
312_F324_L0.7770.76
215_M311_V0.7760.76
123_V185_Q0.7760.76
179_R343_T0.7700.75
238_R271_V0.7660.75
73_W208_R0.7660.75
267_R297_H0.7620.74
30_Y243_A0.7620.74
54_I99_T0.7610.74
257_T263_D0.7590.74
278_Q308_K0.7550.74
253_G277_Y0.7540.74
266_L280_L0.7520.73
91_F114_P0.7510.73
27_F84_Y0.7510.73
245_L272_E0.7470.73
27_F31_Y0.7460.73
163_G169_F0.7460.73
92_R117_E0.7450.73
289_E327_H0.7450.73
222_W226_H0.7430.73
314_D320_K0.7420.72
172_M311_V0.7410.72
262_M280_L0.7400.72
250_D278_Q0.7370.72
242_A273_F0.7360.72
135_R354_I0.7340.72
282_H333_Q0.7320.71
382_A385_R0.7320.71
171_L342_L0.7310.71
242_A272_E0.7310.71
39_F109_I0.7300.71
99_T109_I0.7290.71
237_Q264_A0.7250.71
23_Q120_L0.7250.71
31_Y116_R0.7250.71
263_D267_R0.7210.70
37_A119_I0.7200.70
315_N351_P0.7170.70
39_F354_I0.7160.70
77_L207_A0.7150.70
289_E293_K0.7100.69
111_L118_V0.7090.69
263_D268_D0.7070.69
299_E332_V0.7060.69
115_W119_I0.6990.68
246_E273_F0.6990.68
89_R200_G0.6970.68
263_D266_L0.6960.68
292_E331_R0.6950.68
274_A277_Y0.6910.67
56_E134_H0.6880.67
176_T182_R0.6870.67
201_T212_L0.6860.67
238_R264_A0.6830.66
58_V129_I0.6820.66
102_N191_R0.6810.66
320_K354_I0.6800.66
128_V188_I0.6800.66
224_Q315_N0.6790.66
299_E331_R0.6780.66
52_D195_E0.6780.66
242_A267_R0.6770.65
227_Q363_G0.6770.65
257_T282_H0.6730.65
319_R323_E0.6730.65
106_K384_V0.6720.65
40_R105_G0.6710.65
190_K193_Q0.6710.65
160_L163_G0.6690.65
201_T207_A0.6690.65
11_S14_D0.6670.64
271_V277_Y0.6630.64
151_E191_R0.6600.64
365_P380_D0.6570.63
180_F184_V0.6570.63
29_H226_H0.6570.63
19_K176_T0.6530.63
367_A370_S0.6520.63
242_A275_S0.6520.63
115_W361_C0.6500.62
162_A171_L0.6480.62
69_D72_Q0.6460.62
251_Q254_I0.6450.62
67_Q72_Q0.6430.62
169_F334_L0.6400.61
126_L342_L0.6380.61
173_D198_F0.6380.61
227_Q239_A0.6370.61
268_D272_E0.6360.61
388_R392_D0.6360.61
27_F87_W0.6350.61
97_Q102_N0.6340.60
239_A246_E0.6340.60
57_Q61_M0.6340.60
158_S198_F0.6340.60
192_L195_E0.6330.60
151_E156_D0.6330.60
17_A73_W0.6330.60
173_D212_L0.6320.60
152_S196_S0.6310.60
93_F113_G0.6310.60
135_R352_L0.6280.60
17_A21_H0.6270.60
74_L207_A0.6260.59
202_S340_T0.6260.59
293_K300_K0.6240.59
202_S207_A0.6230.59
324_L333_Q0.6200.59
97_Q111_L0.6190.59
62_Q72_Q0.6180.58
300_K307_S0.6150.58
93_F117_E0.6150.58
185_Q265_F0.6140.58
289_E292_E0.6140.58
288_V291_G0.6120.58
276_R305_P0.6120.58
351_P354_I0.6110.58
13_L16_D0.6090.57
119_I221_E0.6080.57
291_G332_V0.6080.57
169_F351_P0.6060.57
371_D374_G0.6050.57
133_V352_L0.6050.57
291_G327_H0.6040.57
160_L332_V0.6010.56
292_E296_A0.6000.56
52_D151_E0.5990.56
251_Q278_Q0.5990.56
101_S108_D0.5980.56
156_D319_R0.5980.56
133_V179_R0.5980.56
61_M244_W0.5950.56
41_C314_D0.5950.56
367_A376_T0.5940.56
219_A286_D0.5940.56
153_K345_D0.5940.56
266_L301_L0.5930.55
26_V35_V0.5910.55
92_R95_P0.5910.55
289_E320_K0.5900.55
189_V212_L0.5890.55
19_K221_E0.5890.55
184_V343_T0.5890.55
270_G277_Y0.5890.55
40_R377_I0.5880.55
21_H224_Q0.5870.55
340_T347_P0.5860.55
385_R389_K0.5860.55
36_A110_R0.5860.55
176_T202_S0.5840.54
207_A213_T0.5840.54
133_V180_F0.5830.54
313_S369_L0.5820.54
288_V323_E0.5820.54
26_V37_A0.5810.54
277_Y303_I0.5800.54
129_I320_K0.5790.54
309_T335_S0.5780.54
157_F246_E0.5760.53
125_L356_I0.5760.53
187_T190_K0.5750.53
157_F160_L0.5750.53
223_F241_L0.5740.53
249_P276_R0.5730.53
117_E372_S0.5720.53
353_N374_G0.5690.52
289_E323_E0.5680.52
203_N340_T0.5680.52
15_T202_S0.5680.52
50_Y53_A0.5680.52
33_V361_C0.5660.52
31_Y83_D0.5650.52
103_D197_W0.5640.52
37_A118_V0.5640.52
187_T205_D0.5620.51
87_W90_E0.5620.51
194_Q207_A0.5620.51
188_I210_L0.5600.51
35_V225_A0.5600.51
99_T102_N0.5600.51
311_V314_D0.5590.51
336_F370_S0.5590.51
35_V362_N0.5560.51
15_T19_K0.5560.51
254_I278_Q0.5550.51
342_L355_V0.5530.50
369_L376_T0.5520.50
151_E155_V0.5520.50
188_I343_T0.5520.50
367_A371_D0.5510.50
173_D203_N0.5500.50
130_S136_Y0.5490.50
276_R304_D0.5480.50
152_S156_D0.5470.50
97_Q103_D0.5460.49
62_Q92_R0.5440.49
124_P218_Q0.5440.49
137_R342_L0.5430.49
51_A101_S0.5420.49
103_D107_L0.5420.49
107_L299_E0.5420.49
30_Y173_D0.5410.49
25_A226_H0.5390.49
30_Y115_W0.5390.49
27_F81_K0.5380.48
380_D384_V0.5380.48
238_R272_E0.5350.48
57_Q60_A0.5350.48
24_Q27_F0.5320.48
152_S155_V0.5320.48
53_A123_V0.5320.48
20_L24_Q0.5310.48
262_M282_H0.5300.47
315_N341_R0.5300.47
36_A381_K0.5300.47
126_L316_L0.5290.47
57_Q128_V0.5280.47
228_Q287_P0.5280.47
97_Q117_E0.5270.47
153_K317_D0.5210.46
161_T171_L0.5200.46
280_L310_L0.5190.46
382_A386_A0.5190.46
367_A378_C0.5180.46
315_N347_P0.5180.46
24_Q314_D0.5180.46
254_I309_T0.5170.46
17_A79_F0.5170.46
38_E377_I0.5170.46
169_F321_A0.5160.46
69_D209_R0.5160.46
15_T204_Y0.5140.45
99_T112_S0.5130.45
191_R195_E0.5120.45
97_Q109_I0.5110.45
184_V189_V0.5100.45
286_D289_E0.5100.45
94_N296_A0.5090.45
126_L354_I0.5090.45
227_Q364_K0.5090.45
69_D82_A0.5080.45
305_P308_K0.5080.45
154_L158_S0.5070.45
23_Q125_L0.5070.45
26_V225_A0.5060.44
11_S208_R0.5050.44
218_Q244_W0.5050.44
72_Q82_A0.5050.44
271_V275_S0.5050.44
19_K123_V0.5040.44
282_H287_P0.5030.44
292_E378_C0.5030.44
323_E326_R0.5020.44
224_Q251_Q0.5020.44
276_R303_I0.5010.44
23_Q87_W0.5010.44
60_A63_H0.5010.44
53_A135_R0.5000.44
227_Q369_L0.5000.44
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3os4A 1 0.98 100 0.166 Contact Map
4hl7A 4 0.9925 100 0.183 Contact Map
1yirA 1 0.965 100 0.186 Contact Map
1vlpA 1 0.9725 100 0.197 Contact Map
1ybeA 2 0.9525 100 0.209 Contact Map
2im5A 1 0.94 100 0.212 Contact Map
4yubA 2 0.87 100 0.246 Contact Map
4lvfA 2 0.91 100 0.313 Contact Map
4mzyA 2 0.9025 100 0.327 Contact Map
2i14A 5 0.8075 100 0.481 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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