GREMLIN Database
NFSA - Oxygen-insensitive NADPH nitroreductase
UniProt: P17117 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11261
Length: 240 (233)
Sequences: 1621 (1203)
Seq/√Len: 78.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
52_T179_N4.1591.00
116_L126_G3.3771.00
80_C149_V3.2791.00
56_L179_N3.2031.00
60_L139_V3.1751.00
23_S26_Q3.1341.00
8_I117_I2.9421.00
58_E71_A2.9281.00
144_K180_S2.8301.00
81_A104_G2.7791.00
135_N138_A2.6311.00
20_E159_W2.5041.00
229_L233_H2.3761.00
38_S126_G2.3741.00
30_I115_A2.2771.00
17_F156_C2.2561.00
78_V143_L2.2271.00
137_E234_K2.2231.00
50_R75_E2.1471.00
26_Q159_W2.1191.00
116_L155_L2.1101.00
50_R176_V2.0821.00
132_L139_V2.0471.00
3_P6_E2.0411.00
24_E52_T2.0331.00
52_T178_E1.9981.00
79_F115_A1.9861.00
56_L180_S1.9771.00
29_A122_L1.9581.00
18_T161_A1.9181.00
54_K58_E1.9041.00
124_L159_W1.9001.00
194_E198_E1.8821.00
193_D213_S1.8681.00
138_A141_K1.8601.00
55_A58_E1.8591.00
28_E172_A1.8441.00
189_L213_S1.8361.00
140_T235_Q1.7691.00
48_I77_W1.7511.00
112_A128_Y1.7061.00
14_I164_P1.7031.00
77_W115_A1.7001.00
34_A115_A1.6761.00
127_V156_C1.6081.00
140_T231_Y1.6031.00
22_I74_A1.5951.00
37_T110_M1.5871.00
31_I176_V1.5801.00
59_E62_T1.5731.00
84_N183_P1.5670.99
16_H161_A1.5650.99
53_D56_L1.5580.99
26_Q30_I1.5540.99
62_T66_G1.5540.99
9_C29_A1.5400.99
137_E141_K1.5350.99
63_L135_N1.5300.99
63_L138_A1.5060.99
102_L105_V1.4710.99
48_I79_F1.4360.99
26_Q122_L1.4360.99
24_E28_E1.4290.99
32_N35_R1.4130.99
59_E142_L1.4120.99
76_F154_G1.4100.99
6_E10_G1.3800.99
18_T159_W1.3730.99
129_I156_C1.3700.99
134_N225_R1.3660.99
92_D190_A1.3660.99
65_G131_G1.3640.99
199_Y203_R1.3580.99
126_G157_L1.3330.98
53_D179_N1.3150.98
59_E138_A1.2990.98
61_V71_A1.2970.98
46_S111_M1.2830.98
47_S177_H1.2760.98
28_E32_N1.2550.98
30_I119_A1.2330.97
76_F129_I1.2330.97
76_F156_C1.2210.97
48_I111_M1.2070.97
22_I25_A1.1990.97
210_D215_H1.1970.97
26_Q124_L1.1700.96
27_R77_W1.1670.96
38_S116_L1.1640.96
23_S159_W1.1630.96
56_L59_E1.1460.96
147_Q235_Q1.1420.96
56_L142_L1.1350.96
35_R175_L1.1310.95
197_A211_T1.1210.95
116_L128_Y1.0960.95
32_N172_A1.0750.94
27_R31_I1.0750.94
54_K180_S1.0690.94
77_W157_L1.0680.94
227_F230_D1.0650.94
112_A153_F1.0630.94
93_A193_D1.0500.93
193_D211_T1.0380.93
99_E133_R1.0340.93
88_Q91_P1.0330.93
84_N88_Q1.0290.93
230_D234_K1.0290.93
107_D130_G1.0270.92
49_I80_C1.0260.92
182_Q185_D1.0080.92
64_T131_G1.0060.92
198_E201_L0.9980.91
174_I188_A0.9900.91
119_A126_G0.9870.91
101_L104_G0.9770.90
187_G190_A0.9730.90
186_K190_A0.9620.90
4_T7_L0.9620.90
69_H162_D0.9600.90
54_K57_R0.9600.90
36_A166_L0.9570.90
101_L232_L0.9420.89
87_L148_H0.9400.89
16_H162_D0.9340.88
29_A33_S0.9300.88
79_F108_T0.9300.88
87_L91_P0.9250.88
173_S191_Q0.9240.88
48_I176_V0.9220.88
198_E202_T0.9180.88
69_H129_I0.9160.87
11_H114_N0.9150.87
55_A59_E0.9060.87
83_F104_G0.9030.87
31_I50_R0.8990.86
125_G160_P0.8960.86
24_E178_E0.8930.86
46_S175_L0.8900.86
50_R178_E0.8870.86
4_T117_I0.8860.86
11_H120_E0.8830.85
106_V153_F0.8720.85
68_K72_Q0.8710.85
34_A111_M0.8640.84
227_F231_Y0.8640.84
40_S99_E0.8610.84
128_Y153_F0.8610.84
5_I118_A0.8570.84
126_G155_L0.8570.84
111_M115_A0.8560.84
64_T67_Q0.8540.83
61_V64_T0.8490.83
12_R164_P0.8460.83
113_Q128_Y0.8430.83
6_E9_C0.8430.83
9_C32_N0.8380.82
102_L106_V0.8380.82
73_A156_C0.8260.81
5_I29_A0.8210.81
173_S188_A0.8180.81
91_P94_Q0.8150.81
21_P74_A0.8110.80
211_T214_D0.8060.80
161_A164_P0.8060.80
68_K71_A0.8050.80
63_L142_L0.8030.80
9_C33_S0.7980.79
187_G191_Q0.7970.79
165_D204_G0.7950.79
81_A152_L0.7930.79
106_V109_A0.7890.78
52_T75_E0.7880.78
78_V132_L0.7880.78
185_D188_A0.7880.78
28_E173_S0.7740.77
74_A124_L0.7720.77
5_I121_S0.7710.77
11_H126_G0.7700.77
105_V150_L0.7680.76
145_L149_V0.7640.76
8_I33_S0.7590.76
22_I26_Q0.7570.75
56_L144_K0.7550.75
202_T205_S0.7510.75
109_A153_F0.7500.75
8_I118_A0.7480.75
84_N149_V0.7450.74
136_I231_Y0.7450.74
201_L209_R0.7420.74
42_F196_L0.7410.74
201_L218_R0.7280.73
33_S117_I0.7260.72
38_S128_Y0.7240.72
145_L181_Y0.7240.72
230_D233_H0.7240.72
223_E226_P0.7220.72
5_I122_L0.7150.71
86_H216_I0.7140.71
202_T206_N0.7130.71
4_T121_S0.7090.71
61_V66_G0.7060.70
191_Q195_Q0.7060.70
4_T8_I0.7060.70
75_E176_V0.7040.70
45_C103_L0.7010.70
136_I151_P0.7000.70
38_S155_L0.6960.69
64_T70_V0.6880.68
119_A157_L0.6860.68
84_N148_H0.6840.68
46_S109_A0.6770.67
50_R77_W0.6760.67
20_E23_S0.6690.66
58_E61_V0.6680.66
14_I162_D0.6680.66
87_L147_Q0.6670.66
133_R212_W0.6660.66
67_Q70_V0.6580.65
194_E202_T0.6570.65
220_I224_S0.6560.65
5_I33_S0.6550.64
22_I157_L0.6530.64
206_N209_R0.6520.64
25_A28_E0.6520.64
114_N117_I0.6480.64
194_E197_A0.6480.64
26_Q115_A0.6470.63
22_I124_L0.6470.63
118_A121_S0.6460.63
124_L157_L0.6450.63
202_T234_K0.6450.63
177_H182_Q0.6440.63
196_L200_Y0.6440.63
172_A176_V0.6430.63
16_H72_Q0.6380.62
106_V128_Y0.6370.62
226_P230_D0.6370.62
99_E225_R0.6360.62
48_I115_A0.6350.62
191_Q194_E0.6340.62
110_M113_Q0.6330.62
34_A114_N0.6280.61
93_A214_D0.6280.61
50_R57_R0.6270.61
31_I172_A0.6250.61
115_A155_L0.6250.61
49_I194_E0.6230.61
204_G209_R0.6190.60
40_S154_G0.6180.60
196_L215_H0.6180.60
12_R163_N0.6170.60
195_Q198_E0.6170.60
40_S130_G0.6160.60
193_D203_R0.6120.59
132_L151_P0.6120.59
111_M175_L0.6090.59
26_Q29_A0.6070.59
15_R130_G0.6060.58
22_I28_E0.6060.58
147_Q236_G0.6050.58
227_F234_K0.6050.58
200_Y215_H0.6040.58
125_G128_Y0.5990.58
33_S118_A0.5990.58
75_E157_L0.5990.58
200_Y203_R0.5980.57
104_G150_L0.5980.57
105_V109_A0.5970.57
194_E234_K0.5900.56
201_L205_S0.5900.56
109_A113_Q0.5900.56
210_D214_D0.5900.56
205_S214_D0.5900.56
197_A209_R0.5890.56
7_L57_R0.5880.56
57_R75_E0.5860.56
165_D202_T0.5820.55
140_T147_Q0.5800.55
70_V85_R0.5790.55
79_F112_A0.5790.55
31_I37_T0.5790.55
144_K183_P0.5790.55
74_A159_W0.5780.55
73_A76_F0.5760.55
209_R213_S0.5760.55
60_L154_G0.5740.54
42_F192_Y0.5730.54
43_L100_Q0.5700.54
214_D218_R0.5670.54
77_W176_V0.5660.53
40_S103_L0.5660.53
96_G148_H0.5640.53
40_S134_N0.5580.52
11_H38_S0.5570.52
200_Y206_N0.5570.52
199_Y206_N0.5570.52
51_I78_V0.5560.52
81_A149_V0.5520.52
216_I220_I0.5520.52
58_E62_T0.5490.51
38_S153_F0.5470.51
76_F131_G0.5450.51
125_G130_G0.5440.51
35_R176_V0.5440.51
19_D23_S0.5440.51
50_R74_A0.5440.51
216_I222_K0.5420.50
183_P191_Q0.5420.50
40_S69_H0.5410.50
46_S108_T0.5390.50
27_R75_E0.5370.50
215_H220_I0.5340.49
225_R228_I0.5330.49
51_I143_L0.5310.49
88_Q183_P0.5310.49
174_I192_Y0.5290.49
10_G163_N0.5240.48
214_D217_R0.5220.48
22_I122_L0.5220.48
49_I143_L0.5210.48
63_L131_G0.5190.47
17_F69_H0.5190.47
86_H100_Q0.5180.47
41_S128_Y0.5180.47
206_N210_D0.5180.47
18_T162_D0.5170.47
105_V152_L0.5170.47
219_T222_K0.5160.47
102_L232_L0.5160.47
22_I176_V0.5150.47
10_G166_L0.5150.47
193_D196_L0.5140.47
31_I77_W0.5120.46
195_Q218_R0.5120.46
81_A109_A0.5120.46
38_S120_E0.5110.46
69_H128_Y0.5110.46
92_D193_D0.5110.46
11_H117_I0.5100.46
190_A194_E0.5100.46
47_S85_R0.5080.46
30_I155_L0.5070.46
59_E63_L0.5070.46
23_S191_Q0.5040.45
83_F148_H0.5020.45
15_R125_G0.5010.45
57_R73_A0.5010.45
60_L132_L0.5010.45
130_G168_P0.5000.45
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1f5vA 2 1 100 0.198 Contact Map
1bkjA 2 0.9583 100 0.198 Contact Map
3n2sA 2 0.9958 100 0.198 Contact Map
1zchA 2 1 100 0.199 Contact Map
3eofA 2 0.9583 100 0.284 Contact Map
3bemA 2 0.7375 100 0.43 Contact Map
3gagA 4 0.7417 100 0.434 Contact Map
1noxA 2 0.7375 100 0.435 Contact Map
3ge6A 2 0.7375 100 0.438 Contact Map
3gbhA 3 0.7375 100 0.438 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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