GREMLIN Database
CYSP - Thiosulfate-binding protein
UniProt: P16700 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10195
Length: 338 (315)
Sequences: 1398 (807)
Seq/√Len: 45.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
256_Q261_E5.1201.00
162_Y322_H4.5141.00
167_G306_F4.4011.00
278_T295_K4.3791.00
265_K269_N3.8341.00
52_A59_K3.6781.00
76_I85_V3.5371.00
274_P289_N3.2511.00
317_E321_T3.2221.00
278_T296_L3.1851.00
255_V261_E3.0791.00
50_Q54_D3.0611.00
261_E265_K2.9551.00
43_L270_W2.8241.00
181_E331_K2.6311.00
171_K304_E2.5311.00
141_V187_F2.4451.00
176_D179_K2.4321.00
254_N258_N2.4181.00
74_L78_Q2.3941.00
46_P279_I2.3671.00
77_L96_L2.3531.00
60_L263_A2.3431.00
177_K321_T2.2911.00
42_A283_Y2.2791.00
170_D180_T2.2211.00
124_F213_V2.1351.00
182_Q186_Q2.1151.00
186_Q190_N2.0961.00
274_P292_V2.0911.00
276_A280_I2.0841.00
173_D180_T2.0710.99
114_N245_E2.0450.99
173_D176_D2.0390.99
222_N237_I2.0090.99
43_L273_S1.9420.99
26_T58_D1.9380.99
49_Q53_K1.9240.99
256_Q265_K1.9120.99
46_P276_A1.9110.99
146_K192_E1.9020.99
42_A282_D1.8930.99
113_N288_N1.8890.99
168_A183_F1.8630.99
133_N142_R1.8630.99
81_K258_N1.8550.99
138_N168_A1.8520.99
278_T281_T1.8190.99
106_D109_S1.8000.99
148_I192_E1.8000.99
28_L264_A1.7910.98
273_S276_A1.7660.98
170_D177_K1.7580.98
110_R252_D1.7580.98
136_D239_K1.7050.98
51_W58_D1.6780.98
98_D316_P1.6660.98
306_F311_K1.6530.97
180_T184_M1.6520.97
39_L279_I1.6310.97
26_T263_A1.6290.97
185_T189_K1.6270.97
147_L187_F1.6260.97
39_L280_I1.6030.97
294_D297_K1.5780.97
116_S247_P1.5750.97
274_P291_E1.5730.97
25_A59_K1.5510.96
320_K324_T1.5120.96
266_A269_N1.5050.96
110_R249_A1.4990.95
104_P110_R1.4970.95
43_L276_A1.4960.95
252_D261_E1.4730.95
96_L103_I1.4660.95
250_W265_K1.4650.95
146_K190_N1.4650.95
23_V58_D1.4490.94
81_K257_A1.4410.94
97_H103_I1.4380.94
111_L250_W1.4220.94
43_L280_I1.4200.94
27_E63_K1.4090.94
278_T292_V1.3900.93
50_Q270_W1.3850.93
45_P49_Q1.3730.93
252_D255_V1.3610.92
179_K182_Q1.3550.92
309_E314_S1.3490.92
281_T292_V1.3150.91
147_L192_E1.3070.91
308_V314_S1.2980.90
226_K284_Y1.2940.90
81_K254_N1.2810.90
252_D256_Q1.2800.90
173_D179_K1.2750.90
103_I249_A1.2730.90
43_L50_Q1.2710.90
149_F161_T1.2700.89
289_N292_V1.2600.89
128_K234_E1.2490.89
100_G104_P1.2470.89
270_W273_S1.2420.88
103_I115_S1.2330.88
170_D311_K1.2310.88
307_R310_D1.2310.88
21_G25_A1.2070.87
279_I283_Y1.2030.87
124_F215_I1.2010.87
203_T207_E1.1880.86
170_D173_D1.1860.86
138_N173_D1.1800.86
321_T328_E1.1740.85
51_W263_A1.1640.85
297_K302_Q1.1610.85
97_H104_P1.1600.85
296_L299_K1.1500.84
181_E328_E1.1470.84
90_V108_Q1.1450.84
93_V247_P1.1450.84
222_N301_P1.1420.84
97_H106_D1.1370.84
144_D190_N1.1370.84
159_R319_M1.1340.83
28_L263_A1.1270.83
52_A56_G1.1120.82
228_Y284_Y1.1080.82
104_P115_S1.1000.81
314_S317_E1.0880.81
75_A80_L1.0780.80
226_K299_K1.0650.79
50_Q53_K1.0640.79
157_N161_T1.0500.78
312_F318_V1.0480.78
176_D180_T1.0450.78
222_N227_Q1.0440.78
93_V96_L1.0410.78
88_N217_F1.0350.77
94_Q98_D1.0300.77
153_K330_D1.0300.77
292_V296_L1.0300.77
106_D110_R1.0290.77
204_T210_L1.0190.76
143_S190_N1.0190.76
226_K230_A1.0190.76
186_Q189_K1.0060.75
157_N198_G0.9980.75
183_F187_F0.9970.75
166_W184_M0.9960.74
274_P290_P0.9950.74
166_W170_D0.9950.74
111_L249_A0.9930.74
58_D262_K0.9930.74
170_D176_D0.9910.74
50_Q275_Q0.9900.74
237_I301_P0.9900.74
306_F310_D0.9850.74
70_S89_Q0.9850.74
56_G59_K0.9790.73
158_A161_T0.9780.73
118_F246_F0.9680.72
255_V260_T0.9570.71
198_G217_F0.9540.71
70_S160_Y0.9410.70
262_K265_K0.9400.70
89_Q160_Y0.9400.70
77_L95_I0.9390.70
25_A58_D0.9320.69
46_P49_Q0.9310.69
202_T214_L0.9300.69
108_Q247_P0.9270.69
255_V265_K0.9240.69
237_I303_T0.9240.69
111_L269_N0.9190.68
278_T282_D0.9130.68
46_P275_Q0.9130.68
41_A45_P0.9080.67
33_Y157_N0.9020.67
308_V318_V0.9010.67
104_P249_A0.8970.66
143_S186_Q0.8930.66
70_S88_N0.8880.66
35_V217_F0.8840.65
123_G221_V0.8840.65
52_A57_G0.8790.65
141_V190_N0.8710.64
23_V26_T0.8690.64
240_T306_F0.8580.63
88_N198_G0.8480.62
32_S36_S0.8360.61
255_V258_N0.8360.61
87_Y198_G0.8320.61
46_P270_W0.8300.60
26_T60_L0.8270.60
150_P161_T0.8190.59
25_A61_T0.8180.59
207_E228_Y0.8140.59
22_H26_T0.8140.59
104_P111_L0.8130.59
241_N305_L0.8120.59
326_G330_D0.8120.59
100_G253_K0.8090.58
193_V204_T0.8090.58
70_S158_A0.8070.58
49_Q54_D0.8040.58
148_I210_L0.8020.58
202_T206_A0.8010.58
250_W255_V0.7990.57
35_V198_G0.7980.57
41_A207_E0.7960.57
167_G311_K0.7930.57
87_Y217_F0.7930.57
281_T296_L0.7870.56
291_E294_D0.7860.56
225_R228_Y0.7820.56
70_S157_N0.7810.56
110_R265_K0.7790.56
43_L266_A0.7770.55
253_K299_K0.7760.55
36_S198_G0.7750.55
161_T215_I0.7740.55
167_G170_D0.7730.55
222_N284_Y0.7660.54
39_L43_L0.7640.54
216_S224_I0.7630.54
307_R314_S0.7610.54
138_N183_F0.7610.54
317_E320_K0.7560.53
157_N160_Y0.7550.53
109_S302_Q0.7530.53
162_Y184_M0.7510.53
188_L194_F0.7510.53
88_N156_G0.7490.53
125_L224_I0.7480.53
165_A187_F0.7460.52
148_I204_T0.7460.52
290_P294_D0.7450.52
70_S161_T0.7450.52
33_Y86_T0.7400.52
88_N160_Y0.7310.51
97_H105_A0.7310.51
195_D204_T0.7290.51
88_N157_N0.7290.51
22_H58_D0.7270.50
203_T206_A0.7270.50
329_L333_L0.7270.50
248_V271_L0.7250.50
169_A180_T0.7240.50
17_L58_D0.7240.50
167_G240_T0.7230.50
125_L202_T0.7220.50
292_V297_K0.7210.50
96_L102_L0.7210.50
309_E318_V0.7170.49
149_F215_I0.7160.49
217_F246_F0.7130.49
188_L332_L0.7120.49
44_N267_Y0.7110.49
178_G186_Q0.7100.49
201_A324_T0.7100.49
160_Y217_F0.7090.49
282_D296_L0.7090.49
113_N289_N0.7080.49
180_T306_F0.7080.49
305_L309_E0.7060.48
161_T198_G0.7050.48
40_F86_T0.7030.48
144_D204_T0.7030.48
157_N217_F0.7020.48
187_F333_L0.6980.48
110_R250_W0.6970.48
206_A270_W0.6970.48
111_L115_S0.6940.47
184_M332_L0.6890.47
29_L208_R0.6890.47
274_P278_T0.6880.47
50_Q266_A0.6880.47
18_L21_G0.6870.47
89_Q217_F0.6870.47
168_A240_T0.6850.46
174_G249_A0.6840.46
170_D174_G0.6830.46
305_L308_V0.6780.46
132_K212_D0.6770.46
41_A71_K0.6750.45
100_G103_I0.6750.45
157_N201_A0.6740.45
225_R235_V0.6720.45
146_K215_I0.6710.45
190_N201_A0.6710.45
209_G232_G0.6710.45
27_E258_N0.6690.45
252_D265_K0.6660.45
118_F280_I0.6660.45
35_V118_F0.6620.44
168_A239_K0.6610.44
293_M296_L0.6590.44
150_P195_D0.6580.44
87_Y160_Y0.6580.44
18_L23_V0.6570.44
289_N294_D0.6560.44
96_L99_K0.6550.43
169_A175_G0.6540.43
316_P320_K0.6540.43
102_L253_K0.6540.43
206_A230_A0.6500.43
93_V116_S0.6500.43
324_T327_G0.6500.43
80_L159_R0.6470.43
225_R234_E0.6470.43
226_K282_D0.6460.43
177_K180_T0.6450.43
253_K291_E0.6440.42
85_V249_A0.6440.42
54_D265_K0.6390.42
80_L203_T0.6380.42
41_A63_K0.6380.42
166_W180_T0.6370.42
122_M215_I0.6350.42
143_S302_Q0.6350.42
35_V246_F0.6350.42
36_S87_Y0.6330.41
206_A228_Y0.6300.41
41_A64_Q0.6300.41
121_T241_N0.6290.41
208_R229_E0.6230.40
123_G235_V0.6230.40
161_T217_F0.6230.40
30_N44_N0.6230.40
237_I293_M0.6190.40
48_E263_A0.6190.40
48_E60_L0.6180.40
194_F223_N0.6160.40
31_S67_A0.6160.40
43_L46_P0.6150.40
149_F160_Y0.6140.40
116_S288_N0.6140.40
102_L297_K0.6130.39
20_A24_Q0.6110.39
194_F329_L0.6100.39
89_Q161_T0.6070.39
319_M327_G0.6070.39
87_Y158_A0.6060.39
138_N141_V0.6050.39
30_N86_T0.6050.39
151_N195_D0.6040.39
230_A284_Y0.6040.39
117_P248_V0.6030.39
58_D263_A0.6010.38
36_S88_N0.6010.38
51_W266_A0.6000.38
69_S249_A0.5980.38
115_S249_A0.5970.38
234_E327_G0.5970.38
126_V234_E0.5940.38
260_T263_A0.5910.37
108_Q309_E0.5890.37
36_S89_Q0.5870.37
56_G262_K0.5870.37
151_N193_V0.5840.37
89_Q149_F0.5830.37
184_M328_E0.5830.37
88_N158_A0.5810.37
106_D177_K0.5760.36
43_L279_I0.5760.36
207_E325_S0.5740.36
24_Q331_K0.5740.36
222_N235_V0.5740.36
133_N144_D0.5740.36
140_L191_V0.5740.36
122_M217_F0.5730.36
34_D197_G0.5720.36
309_E312_F0.5700.36
45_P196_T0.5700.36
91_T319_M0.5690.35
20_A23_V0.5660.35
206_A284_Y0.5660.35
328_E331_K0.5660.35
36_S217_F0.5650.35
35_V88_N0.5630.35
149_F217_F0.5630.35
28_L62_I0.5620.35
60_L274_P0.5620.35
182_Q190_N0.5610.35
270_W275_Q0.5600.35
21_G58_D0.5600.35
94_Q97_H0.5590.35
250_W261_E0.5590.35
143_S295_K0.5570.34
217_F244_A0.5570.34
100_G105_A0.5560.34
318_V328_E0.5560.34
245_E276_A0.5560.34
95_I99_K0.5550.34
151_N194_F0.5530.34
158_A237_I0.5510.34
70_S217_F0.5500.34
159_R214_L0.5500.34
149_F198_G0.5500.34
88_N246_F0.5490.34
253_K257_A0.5470.34
149_F157_N0.5460.33
85_V96_L0.5460.33
150_P203_T0.5460.33
135_H317_E0.5460.33
97_H249_A0.5450.33
19_L22_H0.5450.33
68_G197_G0.5450.33
24_Q58_D0.5440.33
143_S232_G0.5430.33
90_V93_V0.5430.33
17_L21_G0.5420.33
122_M161_T0.5410.33
195_D200_G0.5410.33
113_N291_E0.5400.33
171_K311_K0.5390.33
178_G182_Q0.5390.33
19_L292_V0.5380.33
53_K109_S0.5380.33
76_I96_L0.5370.33
198_G246_F0.5360.33
262_K266_A0.5360.33
88_N161_T0.5350.32
30_N34_D0.5340.32
121_T303_T0.5330.32
44_N66_H0.5330.32
19_L52_A0.5330.32
88_N149_F0.5310.32
21_G62_I0.5310.32
159_R162_Y0.5300.32
97_H100_G0.5300.32
33_Y40_F0.5290.32
83_D260_T0.5270.32
61_T134_I0.5260.32
35_V285_Y0.5250.32
294_D298_D0.5250.32
70_S308_V0.5250.32
119_Y246_F0.5250.32
91_T316_P0.5240.32
221_V235_V0.5240.32
25_A304_E0.5240.32
87_Y246_F0.5240.32
90_V288_N0.5230.31
166_W322_H0.5220.31
109_S114_N0.5210.31
196_T200_G0.5210.31
163_L323_F0.5210.31
44_N64_Q0.5170.31
170_D184_M0.5160.31
123_G237_I0.5150.31
88_N96_L0.5150.31
86_T117_P0.5130.31
88_N122_M0.5130.31
167_G312_F0.5130.31
193_V326_G0.5120.31
135_H232_G0.5110.30
169_A179_K0.5110.30
232_G257_A0.5090.30
84_V268_L0.5080.30
51_W56_G0.5070.30
126_V236_V0.5060.30
35_V157_N0.5060.30
246_F285_Y0.5050.30
34_D37_R0.5040.30
149_F246_F0.5020.30
203_T318_V0.5020.30
142_R145_V0.5020.30
27_E230_A0.5020.30
250_W268_L0.5010.30
122_M160_Y0.5010.30
89_Q198_G0.5000.30
48_E273_S0.5000.30
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1sbpA 1 0.9142 100 0.612 Contact Map
4jb7A 2 0.6746 100 0.804 Contact Map
4rxlA 1 0.6509 99.9 0.825 Contact Map
4g68A 1 0.8935 99.9 0.834 Contact Map
3r26A 1 0.6509 99.9 0.836 Contact Map
3cg1A 1 0.713 99.9 0.842 Contact Map
2hxwA 2 0.6746 99.9 0.845 Contact Map
3rpwA 1 0.8254 99.9 0.854 Contact Map
4kd5C 3 0.6627 99.9 0.855 Contact Map
3cfzA 1 0.7041 99.9 0.855 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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