GREMLIN Database
HYCD - Formate hydrogenlyase subunit 4
UniProt: P16430 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10477
Length: 307 (288)
Sequences: 5370 (2506)
Seq/√Len: 147.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
154_S159_N4.7331.00
179_L235_F3.9941.00
28_R276_N3.8461.00
233_Q260_A3.6591.00
183_A270_V3.5841.00
21_S42_E3.5071.00
31_L276_N3.4351.00
236_V260_A3.0621.00
233_Q264_L2.6551.00
123_T210_S2.6531.00
43_Y46_I2.4731.00
163_T171_Q2.4561.00
54_S216_G2.4391.00
152_A171_Q2.4141.00
187_F274_F2.3901.00
159_N162_D2.3751.00
182_C235_F2.3541.00
172_S240_I2.3191.00
190_M271_I2.3141.00
240_I267_G2.3021.00
75_M227_K2.2711.00
255_L259_I2.2261.00
232_L267_G2.1411.00
172_S179_L2.0851.00
75_M226_L2.0731.00
71_M110_F2.0531.00
206_E209_L2.0381.00
78_I230_V2.0111.00
63_V113_I1.9871.00
229_L268_V1.9851.00
101_L142_L1.9741.00
50_L218_G1.9581.00
13_L226_L1.9481.00
115_G127_A1.9261.00
186_T231_V1.8511.00
71_M223_G1.8081.00
183_A187_F1.7911.00
99_T103_L1.7891.00
184_F294_F1.7781.00
169_L173_I1.7741.00
77_T99_T1.7721.00
178_A239_F1.7661.00
48_K52_R1.7391.00
27_A269_L1.7181.00
23_I269_L1.7121.00
20_L268_V1.6831.00
266_V270_V1.6581.00
19_L42_E1.6541.00
81_A99_T1.6481.00
147_V178_A1.6111.00
17_A226_L1.5991.00
170_S263_K1.5801.00
156_N159_N1.5791.00
35_R38_G1.5171.00
170_S173_I1.4921.00
27_A272_A1.4861.00
41_Q45_D1.4691.00
130_E198_A1.4681.00
255_L258_V1.4621.00
89_S158_S1.4581.00
30_R35_R1.4541.00
100_L104_F1.4531.00
133_L286_T1.4471.00
175_L305_L1.4411.00
140_M185_A1.4391.00
217_F221_K1.4381.00
31_L273_L1.4301.00
200_A204_L1.4301.00
178_A301_F1.4181.00
209_L217_F1.4141.00
186_T235_F1.4071.00
197_L281_L1.4021.00
7_L11_L1.3731.00
148_A160_I1.3571.00
197_L286_T1.3441.00
70_V74_V1.3361.00
93_Q99_T1.3341.00
273_L277_S1.3211.00
200_A203_E1.3171.00
27_A31_L1.3161.00
17_A222_W1.3121.00
140_M298_F1.3011.00
80_T99_T1.3001.00
145_L238_V1.2901.00
30_R38_G1.2861.00
176_V180_C1.2841.00
16_V229_L1.2551.00
11_L14_F1.2521.00
152_A163_T1.2491.00
65_R216_G1.2471.00
228_Q268_V1.2451.00
26_V30_R1.2361.00
152_A160_I1.2351.00
143_L302_V1.2301.00
235_F267_G1.2281.00
92_P158_S1.2201.00
120_S123_T1.2181.00
205_Q210_S1.2181.00
112_A131_A1.2161.00
188_I195_F1.2121.00
162_D167_W1.1961.00
283_L287_P1.1931.00
126_G198_A1.1831.00
164_V171_Q1.1821.00
159_N163_T1.1821.00
141_L185_A1.1751.00
22_G39_V1.1651.00
158_S162_D1.1581.00
169_L174_P1.1561.00
79_A230_V1.1531.00
182_C231_V1.1491.00
147_V298_F1.1310.99
289_I294_F1.1280.99
64_F68_P1.1190.99
228_Q271_I1.1170.99
151_V175_L1.1160.99
15_A43_Y1.1130.99
114_S117_D1.0880.99
67_T114_S1.0850.99
233_Q237_G1.0740.99
197_L204_L1.0700.99
50_L215_S1.0700.99
16_V265_V1.0690.99
260_A264_L1.0660.99
269_L273_L1.0650.99
92_P156_N1.0650.99
23_I27_A1.0620.99
140_M290_T1.0500.99
244_Q259_I1.0470.99
97_L149_A1.0460.99
77_T103_L1.0450.99
167_W171_Q1.0420.99
47_I51_G1.0360.99
180_C184_F1.0350.99
19_L39_V1.0340.99
204_L208_P1.0300.99
242_W263_K1.0290.99
24_T275_E1.0230.99
197_L285_I1.0200.99
67_T113_I1.0200.99
72_V76_L1.0190.99
143_L299_L1.0180.99
236_V240_I1.0170.99
43_Y47_I1.0100.99
190_M231_V1.0090.99
32_H37_P1.0060.99
179_L239_F1.0000.99
215_S218_G0.9910.99
114_S220_M0.9880.99
236_V264_L0.9880.99
26_V52_R0.9870.99
24_T192_K0.9860.99
100_L157_I0.9860.99
57_P64_F0.9840.99
56_G65_R0.9810.99
114_S212_Y0.9810.99
196_D280_R0.9800.98
181_A297_A0.9790.98
140_M295_G0.9740.98
154_S160_I0.9720.98
241_P263_K0.9710.98
108_R134_G0.9680.98
71_M220_M0.9670.98
128_S198_A0.9660.98
270_V274_F0.9660.98
22_G42_E0.9630.98
81_A100_L0.9610.98
67_T117_D0.9600.98
147_V302_V0.9570.98
67_T110_F0.9460.98
267_G270_V0.9380.98
11_L15_A0.9290.98
281_L285_I0.9170.98
282_R287_P0.9150.98
72_V223_G0.9140.98
75_M230_V0.9130.98
200_A280_R0.9130.98
21_S225_S0.9110.98
26_V38_G0.9090.98
147_V175_L0.9070.98
183_A271_I0.9040.97
273_L278_M0.9020.97
240_I263_K0.9020.97
46_I51_G0.9010.97
23_I272_A0.8920.97
176_V179_L0.8890.97
74_V103_L0.8870.97
175_L301_F0.8870.97
144_G185_A0.8830.97
28_R275_E0.8750.97
301_F305_L0.8700.97
46_I50_L0.8680.97
101_L145_L0.8650.97
109_F283_L0.8640.97
72_V219_V0.8610.97
164_V241_P0.8590.97
144_G181_A0.8580.97
49_L218_G0.8530.96
81_A96_D0.8470.96
46_I222_W0.8380.96
29_A35_R0.8380.96
130_E200_A0.8260.96
49_L221_K0.8250.96
89_S92_P0.8230.96
96_D156_N0.8210.96
116_L119_G0.8200.95
84_V90_P0.8190.95
140_M294_F0.8160.95
193_L278_M0.8100.95
297_A301_F0.8080.95
63_V67_T0.8080.95
183_A235_F0.8060.95
86_T162_D0.8040.95
93_Q220_M0.8020.95
32_H36_G0.8020.95
192_K196_D0.8000.95
164_V170_S0.7960.95
14_F76_L0.7910.95
187_F271_I0.7890.94
22_G30_R0.7870.94
59_A64_F0.7850.94
133_L191_G0.7820.94
196_D199_E0.7820.94
90_P222_W0.7800.94
196_D203_E0.7750.94
295_G298_F0.7700.94
188_I286_T0.7690.94
260_A263_K0.7690.94
70_V73_G0.7580.93
133_L137_V0.7560.93
22_G28_R0.7530.93
203_E280_R0.7520.93
144_G147_V0.7510.93
97_L157_I0.7490.93
256_A259_I0.7460.93
45_D48_K0.7420.92
19_L43_Y0.7420.92
170_S241_P0.7390.92
172_S176_V0.7390.92
209_L221_K0.7390.92
40_L43_Y0.7370.92
149_A155_T0.7370.92
73_G304_L0.7350.92
181_A184_F0.7330.92
10_A14_F0.7290.92
218_G222_W0.7270.92
77_T159_N0.7260.92
65_R69_Y0.7230.91
189_E192_K0.7220.91
21_S24_T0.7210.91
104_F107_A0.7200.91
72_V210_S0.7190.91
157_I234_M0.7180.91
183_A267_G0.7170.91
182_C239_F0.7160.91
264_L268_V0.7140.91
97_L160_I0.7130.91
202_Q206_E0.7110.91
56_G61_G0.7100.91
231_V235_F0.7090.91
118_T212_Y0.7080.91
257_L261_I0.7080.91
236_V263_K0.7070.91
29_A34_R0.7060.90
182_C186_T0.7030.90
179_L240_I0.7030.90
74_V107_A0.7030.90
9_Q90_P0.7000.90
24_T225_S0.6980.90
18_P46_I0.6970.90
16_V19_L0.6960.90
18_P28_R0.6950.90
136_L287_P0.6940.90
71_M107_A0.6940.90
198_A208_P0.6930.90
45_D280_R0.6910.90
202_Q205_Q0.6890.89
258_V262_V0.6880.89
37_P41_Q0.6870.89
28_R33_N0.6870.89
284_D287_P0.6870.89
221_K275_E0.6860.89
32_H211_E0.6850.89
214_G218_G0.6840.89
82_L233_Q0.6830.89
41_Q48_K0.6830.89
262_V265_V0.6820.89
93_Q100_L0.6810.89
120_S271_I0.6750.88
280_R284_D0.6730.88
196_D204_L0.6710.88
71_M111_F0.6700.88
57_P68_P0.6680.88
16_V268_V0.6680.88
167_W172_S0.6680.88
147_V301_F0.6660.88
137_V188_I0.6650.88
25_R34_R0.6640.88
236_V256_A0.6620.87
141_L144_G0.6600.87
82_L90_P0.6580.87
26_V39_V0.6570.87
15_A18_P0.6570.87
126_G130_E0.6530.87
147_V305_L0.6530.87
177_L301_F0.6530.87
110_F113_I0.6500.86
25_R36_G0.6470.86
288_R292_A0.6460.86
268_V272_A0.6460.86
133_L136_L0.6450.86
64_F212_Y0.6430.86
52_R292_A0.6420.86
86_T89_S0.6410.86
193_L202_Q0.6370.85
19_L22_G0.6360.85
289_I293_G0.6330.85
18_P22_G0.6310.85
17_A29_A0.6300.85
25_R45_D0.6280.85
84_V233_Q0.6280.85
123_T206_E0.6270.84
144_G178_A0.6250.84
244_Q255_L0.6210.84
281_L290_T0.6190.84
46_I218_G0.6180.84
54_S64_F0.6170.84
141_L290_T0.6140.83
266_V269_L0.6140.83
6_P91_L0.6140.83
126_G205_Q0.6130.83
92_P96_D0.6090.83
5_Y131_A0.6090.83
173_I176_V0.6080.83
142_L145_L0.6080.83
31_L269_L0.6080.83
177_L181_A0.6080.83
60_S119_G0.6070.83
82_L230_V0.6070.83
199_E207_G0.6060.82
68_P212_Y0.6050.82
197_L200_A0.6050.82
206_E221_K0.6040.82
113_I143_L0.6040.82
171_Q174_P0.5980.82
160_I238_V0.5970.81
104_F235_F0.5970.81
25_R199_E0.5950.81
162_D168_P0.5920.81
76_L226_L0.5920.81
81_A84_V0.5890.81
34_R207_G0.5870.80
74_V106_I0.5860.80
66_L70_V0.5850.80
298_F305_L0.5820.80
47_I50_L0.5810.80
40_L44_R0.5810.80
24_T28_R0.5810.80
22_G26_V0.5790.79
13_L17_A0.5750.79
168_P172_S0.5740.79
237_G242_W0.5730.79
90_P95_G0.5720.79
9_Q84_V0.5710.79
96_D103_L0.5700.78
97_L145_L0.5670.78
73_G162_D0.5650.78
136_L275_E0.5650.78
234_M238_V0.5630.78
42_E272_A0.5620.77
208_P221_K0.5610.77
17_A28_R0.5590.77
131_A140_M0.5590.77
118_T199_E0.5590.77
111_F130_E0.5590.77
151_V174_P0.5580.77
201_E221_K0.5560.77
192_K225_S0.5550.77
44_R121_P0.5540.76
187_F231_V0.5530.76
286_T295_G0.5470.76
113_I117_D0.5460.75
146_W149_A0.5460.75
272_A275_E0.5450.75
118_T123_T0.5440.75
111_F191_G0.5420.75
90_P303_S0.5420.75
136_L295_G0.5420.75
144_G239_F0.5410.75
187_F190_M0.5390.75
16_V20_L0.5380.74
30_R272_A0.5380.74
64_F114_S0.5370.74
30_R142_L0.5370.74
27_A273_L0.5350.74
25_R201_E0.5340.74
236_V267_G0.5340.74
134_G142_L0.5330.74
232_L271_I0.5330.74
261_I265_V0.5320.74
21_S46_I0.5290.73
25_R32_H0.5280.73
129_R207_G0.5240.72
180_C302_V0.5230.72
282_R285_I0.5230.72
225_S275_E0.5200.72
58_D113_I0.5200.72
300_A304_L0.5170.71
127_A223_G0.5170.71
104_F134_G0.5170.71
84_V261_I0.5170.71
259_I270_V0.5170.71
14_F18_P0.5160.71
232_L236_V0.5150.71
16_V226_L0.5150.71
183_A273_L0.5150.71
176_V301_F0.5130.71
272_A276_N0.5120.71
53_Q64_F0.5120.71
199_E203_E0.5080.70
189_E224_I0.5060.70
85_V244_Q0.5060.70
225_S286_T0.5060.70
190_M227_K0.5050.70
147_V171_Q0.5040.70
233_Q298_F0.5030.69
197_L280_R0.5020.69
262_V266_V0.5020.69
71_M78_I0.5010.69
18_P42_E0.5010.69
110_F127_A0.5000.69
280_R283_L0.5000.69
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4he8H 1 0.8176 100 0.076 Contact Map
1q2fA 1 0.101 6.5 0.97 Contact Map
2jpkA 1 0.1107 3.7 0.973 Contact Map
4uisB 1 0.1336 2.9 0.975 Contact Map
2m7gA 1 0.1954 2.7 0.975 Contact Map
4he8M 1 0.9088 2.6 0.975 Contact Map
4ogqG 1 0.1205 2.6 0.975 Contact Map
1vryA 1 0.1792 2.6 0.975 Contact Map
1q90G 1 0.0977 2.6 0.975 Contact Map
2kncA 1 0.1498 2.1 0.976 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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