GREMLIN Database
MCRB - 5-methylcytosine-specific restriction enzyme B
UniProt: P15005 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10574
Length: 459 (381)
Sequences: 1452 (1143)
Seq/√Len: 58.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
215_A227_V3.9191.00
163_S166_E3.7201.00
363_Q368_L3.3171.00
364_F369_L3.3151.00
363_Q370_N3.1981.00
368_L372_K3.1891.00
134_Y148_Y3.1451.00
183_T355_I2.9231.00
365_R372_K2.9161.00
363_Q366_N2.8171.00
365_R369_L2.7731.00
134_Y138_A2.7481.00
303_G309_P2.7381.00
363_Q372_K2.6081.00
365_R368_L2.4861.00
366_N372_K2.4851.00
366_N370_N2.4781.00
137_F148_Y2.4691.00
366_N369_L2.4481.00
363_Q369_L2.4351.00
131_G134_Y2.4281.00
138_A148_Y2.3961.00
364_F370_N2.3781.00
364_F367_F2.3661.00
364_F368_L2.3371.00
364_F372_K2.3371.00
367_F370_N2.3291.00
425_L435_P2.2971.00
364_F371_K2.2841.00
144_I148_Y2.2421.00
369_L372_K2.1731.00
138_A142_D2.1641.00
367_F371_K2.1601.00
366_N371_K2.1141.00
237_S243_Q2.0631.00
365_R371_K2.0191.00
131_G138_A1.9861.00
385_M410_F1.9841.00
303_G310_L1.9781.00
304_E309_P1.9681.00
131_G148_Y1.9331.00
284_A289_V1.9281.00
135_T148_Y1.8971.00
304_E310_L1.8801.00
365_R370_N1.8791.00
132_I138_A1.8771.00
137_F142_D1.8671.00
138_A141_L1.8391.00
419_S423_Q1.8130.99
137_F144_I1.8060.99
191_L327_Y1.8020.99
127_K130_Q1.7970.99
116_K119_Q1.7960.99
367_F372_K1.7830.99
308_V311_T1.7780.99
131_G137_F1.7750.99
286_L340_A1.7660.99
148_Y151_I1.7450.99
135_T138_A1.7390.99
134_Y137_F1.7140.99
132_I136_R1.7020.99
19_R23_T1.7010.99
363_Q367_F1.6900.99
303_G311_T1.6850.99
133_D148_Y1.6830.99
82_I92_W1.6800.99
363_Q371_K1.6780.99
104_E108_A1.6710.99
128_V134_Y1.6590.99
60_S70_Y1.6370.99
131_G144_I1.6090.99
79_A82_I1.6080.99
113_Y118_G1.6070.99
132_I141_L1.5750.99
79_A92_W1.5300.98
421_D425_L1.5040.98
131_G142_D1.4960.98
430_M435_P1.4940.98
134_Y144_I1.4810.98
420_P425_L1.4800.98
132_I144_I1.4680.98
135_T142_D1.4620.98
66_V72_D1.4540.97
306_W310_L1.4530.97
187_I197_I1.4520.97
133_D137_F1.4200.97
303_G308_V1.4050.97
82_I90_A1.4000.97
40_G49_W1.3960.97
137_F151_I1.3960.97
431_T436_L1.3920.97
428_I436_L1.3860.97
141_L145_I1.3830.97
33_L41_Y1.3790.97
145_I148_Y1.3750.96
113_Y116_K1.3720.96
112_V116_K1.3640.96
81_G92_W1.3600.96
128_V138_A1.3580.96
421_D424_W1.3560.96
116_K120_S1.3500.96
304_E308_V1.3490.96
338_S343_D1.3490.96
137_F140_M1.3490.96
191_L329_I1.3450.96
65_P70_Y1.3410.96
92_W95_S1.3360.96
141_L144_I1.3350.96
418_T421_D1.3110.95
139_S147_D1.3070.95
111_G121_Y1.3020.95
133_D144_I1.2980.95
428_I431_T1.2870.95
220_G323_P1.2830.95
428_I433_I1.2750.95
33_L44_F1.2720.94
386_N390_Q1.2700.94
98_I102_I1.2690.94
303_G307_S1.2660.94
131_G135_T1.2580.94
141_L148_Y1.2520.94
196_N329_I1.2400.94
134_Y141_L1.2330.94
31_R34_R1.2290.93
427_E431_T1.2270.93
211_A277_I1.2210.93
49_W52_F1.2150.93
18_Q21_Q1.2110.93
335_A341_V1.2080.93
112_V120_S1.2000.93
110_S114_P1.1950.92
110_S113_Y1.1890.92
62_G65_P1.1720.92
114_P120_S1.1650.91
142_D145_I1.1620.91
84_D95_S1.1610.91
113_Y120_S1.1580.91
67_I71_K1.1530.91
58_E63_I1.1510.91
118_G123_A1.1500.91
25_D29_S1.1490.91
59_A66_V1.1490.91
124_C127_K1.1450.91
20_S23_T1.1360.90
131_G147_D1.1310.90
132_I135_T1.1300.90
63_I69_Y1.1300.90
426_N431_T1.1160.90
27_P30_Y1.1120.89
99_P103_A1.1100.89
27_P34_R1.1050.89
169_C174_L1.1010.89
79_A108_A1.0950.89
297_M301_K1.0930.89
123_A126_Q1.0920.89
407_H411_C1.0910.88
32_N36_K1.0910.88
41_Y45_T1.0890.88
131_G139_S1.0860.88
59_A70_Y1.0850.88
117_Y120_S1.0820.88
62_G66_V1.0800.88
62_G72_D1.0790.88
136_R140_M1.0790.88
132_I148_Y1.0770.88
417_G421_D1.0740.88
140_M145_I1.0740.88
421_D428_I1.0650.87
30_Y33_L1.0630.87
65_P72_D1.0610.87
49_W53_L1.0550.87
145_I151_I1.0430.86
66_V73_F1.0420.86
135_T139_S1.0420.86
119_Q126_Q1.0390.86
48_P51_A1.0380.86
137_F149_K1.0370.86
42_G46_S1.0330.86
63_I68_L1.0280.85
134_Y145_I1.0270.85
40_G44_F1.0270.85
63_I71_K1.0190.85
69_Y74_D1.0160.85
135_T145_I1.0140.85
95_S102_I1.0100.84
343_D350_F1.0090.84
50_F56_G1.0080.84
368_L371_K1.0070.84
41_Y47_I1.0040.84
116_K121_Y1.0030.84
111_G116_K0.9960.83
41_Y54_G0.9920.83
105_Y108_A0.9870.83
130_Q137_F0.9860.83
427_E430_M0.9860.83
114_P118_G0.9850.83
23_T27_P0.9840.83
43_N47_I0.9830.83
137_F141_L0.9830.83
26_Y33_L0.9810.83
124_C131_G0.9780.82
42_G45_T0.9760.82
48_P64_Y0.9740.82
122_Y127_K0.9730.82
121_Y138_A0.9730.82
92_W105_Y0.9690.82
429_V433_I0.9670.82
138_A144_I0.9660.82
123_A130_Q0.9650.81
62_G69_Y0.9650.81
141_L151_I0.9630.81
433_I436_L0.9600.81
282_N333_N0.9590.81
428_I435_P0.9590.81
90_A96_S0.9510.80
236_Y245_Y0.9470.80
141_L150_L0.9440.80
430_M436_L0.9430.80
63_I66_V0.9430.80
134_Y140_M0.9410.80
38_S48_P0.9400.80
40_G45_T0.9350.79
96_S106_F0.9330.79
112_V118_G0.9310.79
134_Y147_D0.9300.79
135_T144_I0.9290.79
144_I151_I0.9270.79
103_A107_Q0.9260.79
426_N433_I0.9260.79
124_C128_V0.9250.79
297_M328_I0.9250.79
208_T350_F0.9230.78
29_S33_L0.9220.78
425_L428_I0.9220.78
42_G62_G0.9190.78
189_K245_Y0.9180.78
307_S310_L0.9110.78
124_C138_A0.9110.78
90_A93_Q0.9100.77
58_E72_D0.9060.77
138_A145_I0.9030.77
38_S47_I0.9020.77
421_D427_E0.8980.77
63_I70_Y0.8950.76
169_C180_P0.8940.76
23_T30_Y0.8930.76
126_Q132_I0.8910.76
132_I151_I0.8850.75
38_S42_G0.8850.75
18_Q23_T0.8850.75
64_Y67_I0.8850.75
110_S138_A0.8830.75
135_T141_L0.8810.75
30_Y41_Y0.8790.75
60_S64_Y0.8750.75
295_M298_E0.8750.75
70_Y74_D0.8740.75
362_P369_L0.8730.74
264_Q267_K0.8700.74
198_I211_A0.8690.74
43_N49_W0.8670.74
426_N432_D0.8660.74
419_S424_W0.8640.74
64_Y68_L0.8620.74
114_P119_Q0.8620.74
427_E435_P0.8600.73
339_L343_D0.8570.73
124_C130_Q0.8550.73
43_N46_S0.8530.73
111_G114_P0.8530.73
124_C129_S0.8500.73
114_P121_Y0.8490.72
36_K55_E0.8450.72
203_P334_T0.8450.72
126_Q138_A0.8430.72
233_H292_E0.8420.72
332_M335_A0.8400.72
36_K39_F0.8390.72
245_Y276_F0.8350.71
19_R34_R0.8330.71
57_Q70_Y0.8330.71
19_R27_P0.8300.71
63_I73_F0.8210.70
400_G403_F0.8200.70
18_Q28_T0.8200.70
432_D435_P0.8190.70
93_Q96_S0.8190.70
18_Q30_Y0.8180.70
48_P53_L0.8170.69
430_M433_I0.8160.69
89_H96_S0.8150.69
127_K134_Y0.8120.69
126_Q135_T0.8110.69
49_W54_G0.8100.69
116_K123_A0.8050.68
82_I104_E0.8040.68
413_G417_G0.8020.68
43_N64_Y0.8000.68
339_L349_R0.7940.67
34_R51_A0.7910.67
63_I72_D0.7850.66
30_Y44_F0.7840.66
389_N403_F0.7820.66
304_E307_S0.7820.66
402_G407_H0.7780.66
424_W427_E0.7780.66
425_L430_M0.7770.66
311_T326_V0.7750.65
425_L431_T0.7730.65
31_R35_V0.7730.65
421_D435_P0.7720.65
78_L81_G0.7670.65
61_N67_I0.7660.64
47_I53_L0.7630.64
33_L49_W0.7630.64
19_R22_S0.7600.64
41_Y46_S0.7600.64
127_K132_I0.7580.64
23_T34_R0.7570.64
211_A329_I0.7570.64
48_P54_G0.7520.63
129_S137_F0.7520.63
304_E311_T0.7510.63
43_N56_G0.7480.63
431_T435_P0.7470.63
391_E401_K0.7460.62
40_G48_P0.7420.62
307_S311_T0.7420.62
46_S68_L0.7420.62
128_V135_T0.7410.62
92_W99_P0.7410.62
23_T41_Y0.7410.62
137_F150_L0.7400.62
112_V119_Q0.7390.62
43_N53_L0.7390.62
110_S127_K0.7390.62
426_N430_M0.7380.62
89_H92_W0.7380.62
334_T339_L0.7380.62
143_N147_D0.7370.61
22_S33_L0.7350.61
83_S93_Q0.7330.61
284_A292_E0.7330.61
345_A355_I0.7300.61
58_E61_N0.7300.61
406_G410_F0.7240.60
110_S118_G0.7210.60
132_I142_D0.7190.60
125_S128_V0.7180.59
70_Y73_F0.7170.59
126_Q130_Q0.7170.59
87_E90_A0.7150.59
21_Q25_D0.7130.59
78_L92_W0.7120.59
109_T113_Y0.7100.59
58_E62_G0.7100.59
239_E264_Q0.7100.59
127_K139_S0.7090.59
140_M144_I0.7090.59
236_Y242_I0.7080.58
339_L404_R0.7080.58
85_T101_T0.7050.58
134_Y142_D0.7040.58
24_K28_T0.7030.58
180_P183_T0.7030.58
75_E90_A0.7020.58
56_G60_S0.6980.57
124_C135_T0.6970.57
114_P117_Y0.6970.57
81_G93_Q0.6950.57
240_D301_K0.6930.57
99_P106_F0.6920.57
132_I137_F0.6920.57
116_K124_C0.6880.56
341_V344_Y0.6840.56
48_P52_F0.6840.56
134_Y149_K0.6830.56
140_M151_I0.6810.55
85_T89_H0.6800.55
26_Y30_Y0.6800.55
220_G325_N0.6790.55
118_G124_C0.6790.55
45_T48_P0.6780.55
236_Y288_K0.6770.55
146_N149_K0.6760.55
65_P69_Y0.6730.55
130_Q133_D0.6730.55
294_M345_A0.6700.54
93_Q106_F0.6690.54
85_T92_W0.6690.54
418_T425_L0.6670.54
200_Q381_L0.6650.54
131_G141_L0.6630.53
424_W428_I0.6620.53
87_E91_Q0.6620.53
111_G128_V0.6620.53
21_Q31_R0.6620.53
22_S44_F0.6600.53
69_Y73_F0.6580.53
63_I67_I0.6570.53
19_R41_Y0.6560.53
187_I193_I0.6550.53
244_G295_M0.6540.52
46_S50_F0.6530.52
65_P71_K0.6520.52
205_V282_N0.6510.52
169_C175_N0.6510.52
132_I139_S0.6500.52
40_G52_F0.6490.52
188_L192_T0.6490.52
113_Y117_Y0.6480.52
124_C133_D0.6480.52
338_S345_A0.6470.52
202_P346_L0.6470.52
92_W96_S0.6450.51
85_T107_Q0.6450.51
38_S43_N0.6440.51
145_I149_K0.6430.51
132_I140_M0.6420.51
116_K128_V0.6420.51
360_D389_N0.6410.51
97_D102_I0.6400.51
121_Y126_Q0.6390.51
119_Q123_A0.6390.51
109_T141_L0.6380.51
173_A178_F0.6370.51
20_S47_I0.6360.50
119_Q124_C0.6350.50
174_L180_P0.6340.50
137_F147_D0.6330.50
82_I85_T0.6330.50
48_P66_V0.6320.50
132_I145_I0.6300.50
44_F54_G0.6280.50
123_A138_A0.6270.49
75_E80_Y0.6260.49
234_Q284_A0.6260.49
75_E89_H0.6250.49
65_P68_L0.6250.49
238_Y243_Q0.6240.49
63_I74_D0.6200.49
135_T150_L0.6200.49
52_F56_G0.6150.48
198_I330_G0.6150.48
60_S67_I0.6150.48
133_D140_M0.6140.48
19_R30_Y0.6130.48
60_S65_P0.6130.48
27_P33_L0.6130.48
427_E433_I0.6110.48
128_V132_I0.6100.48
29_S38_S0.6100.48
59_A84_D0.6090.47
49_W56_G0.6090.47
138_A149_K0.6080.47
161_P164_K0.6070.47
284_A300_D0.6030.47
335_A344_Y0.6020.47
58_E73_F0.6020.47
216_Y284_A0.6010.47
76_L105_Y0.6010.47
40_G51_A0.6010.47
280_E333_N0.6010.47
20_S24_K0.6000.47
28_T66_V0.6000.47
41_Y53_L0.5990.46
118_G138_A0.5980.46
263_C326_V0.5970.46
305_N308_V0.5970.46
59_A62_G0.5960.46
403_F406_G0.5950.46
96_S105_Y0.5940.46
236_Y344_Y0.5920.46
127_K140_M0.5910.46
115_K120_S0.5880.45
138_A150_L0.5860.45
24_K39_F0.5850.45
118_G128_V0.5840.45
402_G410_F0.5830.45
94_F102_I0.5820.45
33_L40_G0.5810.44
121_Y130_Q0.5800.44
421_D430_M0.5800.44
87_E108_A0.5800.44
37_L45_T0.5800.44
281_I290_F0.5790.44
43_N51_A0.5790.44
196_N327_Y0.5790.44
53_L62_G0.5750.44
343_D347_R0.5750.44
417_G423_Q0.5730.44
123_A128_V0.5720.43
345_A350_F0.5720.43
82_I87_E0.5690.43
28_T35_V0.5690.43
79_A95_S0.5680.43
138_A147_D0.5660.43
62_G68_L0.5650.43
33_L55_E0.5630.42
42_G76_L0.5630.42
132_I147_D0.5620.42
23_T36_K0.5620.42
109_T112_V0.5620.42
360_D403_F0.5590.42
339_L344_Y0.5560.42
117_Y126_Q0.5540.41
117_Y134_Y0.5530.41
121_Y134_Y0.5530.41
91_Q102_I0.5510.41
110_S121_Y0.5500.41
233_H294_M0.5500.41
31_R41_Y0.5500.41
93_Q105_Y0.5500.41
18_Q22_S0.5490.41
88_P92_W0.5480.41
113_Y119_Q0.5480.41
44_F48_P0.5470.41
130_Q138_A0.5460.41
47_I51_A0.5460.41
38_S41_Y0.5450.41
29_S47_I0.5440.40
343_D348_R0.5430.40
113_Y136_R0.5420.40
95_S106_F0.5410.40
337_R340_A0.5410.40
294_M356_E0.5400.40
233_H240_D0.5400.40
383_Q411_C0.5390.40
136_R142_D0.5380.40
293_V301_K0.5350.39
61_N66_V0.5350.39
79_A93_Q0.5330.39
23_T48_P0.5330.39
34_R47_I0.5330.39
115_K126_Q0.5320.39
79_A88_P0.5320.39
39_F43_N0.5310.39
84_D88_P0.5310.39
424_W435_P0.5310.39
26_Y44_F0.5310.39
36_K54_G0.5290.39
44_F75_E0.5290.39
334_T338_S0.5250.38
24_K34_R0.5250.38
339_L346_L0.5240.38
136_R143_N0.5240.38
20_S44_F0.5220.38
121_Y124_C0.5210.38
25_D36_K0.5200.38
230_V237_S0.5160.37
19_R43_N0.5150.37
51_A56_G0.5150.37
246_R344_Y0.5140.37
67_I75_E0.5130.37
25_D41_Y0.5130.37
381_L412_C0.5120.37
111_G138_A0.5110.37
203_P336_D0.5110.37
202_P205_V0.5100.37
38_S63_I0.5100.37
285_N288_K0.5090.37
77_V90_A0.5090.37
20_S42_G0.5080.37
59_A69_Y0.5070.37
236_Y240_D0.5060.36
385_M409_Y0.5050.36
92_W108_A0.5050.36
116_K130_Q0.5050.36
427_E436_L0.5050.36
167_S178_F0.5040.36
69_Y72_D0.5030.36
39_F54_G0.5000.36
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3vkgA 2 0.8954 100 0.54 Contact Map
4akgA 2 0.8889 100 0.557 Contact Map
4rh7A 1 0.8954 99.9 0.743 Contact Map
2r44A 1 0.6078 99.8 0.789 Contact Map
1qvrA 3 0.8235 99.6 0.815 Contact Map
3nbxX 1 0.5752 99.6 0.815 Contact Map
1r6bX 1 0.7407 99.6 0.818 Contact Map
4d2uA 3 0.8148 99.6 0.819 Contact Map
3pxiA 3 0.7211 99.6 0.821 Contact Map
4ciuA 1 0.8192 99.6 0.822 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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