GREMLIN Database
PROW - Glycine betaine/L-proline transport system permease protein ProW
UniProt: P14176 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10772
Length: 354 (271)
Sequences: 1969 (1188)
Seq/√Len: 72.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
249_R253_F5.7811.00
241_S255_V5.4121.00
169_W176_A4.5641.00
114_A130_L4.1301.00
242_R251_M4.0211.00
172_R256_Q3.7231.00
132_S315_I3.5041.00
177_A227_I3.3911.00
227_I231_N3.2041.00
241_S251_M3.1691.00
175_R179_I2.8821.00
176_A180_I2.8761.00
104_M109_A2.8661.00
75_V83_F2.7851.00
174_P231_N2.6391.00
82_V86_V2.5681.00
293_M309_G2.4841.00
113_F129_T2.4401.00
187_M216_I2.2831.00
144_Q293_M2.2131.00
179_I183_L2.2101.00
63_P67_W2.1441.00
160_I268_V2.0491.00
166_L223_I1.9581.00
107_P111_I1.9491.00
115_L119_Q1.9361.00
251_M255_V1.9241.00
85_G89_P1.9211.00
175_R178_K1.9121.00
170_L227_I1.9081.00
109_A112_V1.8701.00
114_A126_G1.8571.00
166_L180_I1.8361.00
170_L176_A1.7861.00
110_I137_G1.7841.00
64_L135_A1.7641.00
129_T319_I1.7431.00
133_L319_I1.7391.00
124_G128_A1.7261.00
302_D305_L1.7131.00
81_P85_G1.7021.00
118_W126_G1.6861.00
121_S125_M1.6841.00
126_G129_T1.6701.00
123_V127_V1.6280.99
174_P227_I1.6190.99
91_D95_N1.6000.99
158_F215_I1.5920.99
100_L103_G1.5900.99
284_M295_L1.5890.99
98_Q147_V1.5610.99
98_Q207_N1.5430.99
99_Q103_G1.5330.99
230_I256_Q1.5320.99
185_D224_R1.5090.99
222_I271_T1.4790.99
65_D308_V1.4770.99
238_I256_Q1.4690.99
172_R248_P1.4480.99
170_L180_I1.4470.99
157_L161_V1.4110.98
295_L298_I1.3900.98
247_S250_Q1.3760.98
103_G112_V1.3740.98
125_M128_A1.3710.98
109_A146_M1.3530.98
283_S295_L1.3520.98
177_A181_R1.3510.98
197_V306_A1.3440.98
97_F154_T1.3390.98
139_I297_G1.3260.98
234_P259_L1.3250.98
100_L104_M1.3230.98
173_S176_A1.3120.98
217_F277_S1.2910.97
124_G127_V1.2910.97
160_I164_L1.2830.97
113_F133_L1.2800.97
242_R248_P1.2790.97
238_I251_M1.2750.97
61_L138_A1.2730.97
134_I137_G1.2620.97
297_G309_G1.2610.97
323_L326_A1.2550.97
92_Y96_G1.2540.97
74_W78_H1.2400.97
104_M108_V1.2380.96
102_L146_M1.2290.96
288_G292_Q1.2270.96
187_M220_P1.2140.96
232_Q263_T1.2010.96
98_Q102_L1.1990.96
64_L67_W1.1960.96
127_V131_V1.1890.96
90_V212_V1.1830.95
147_V288_G1.1810.95
64_L139_I1.1800.95
135_A139_I1.1770.95
114_A117_A1.1750.95
199_I202_L1.1730.95
136_I312_G1.1530.95
117_A323_L1.1500.95
65_D69_T1.1470.95
234_P254_K1.1260.94
95_N143_S1.1200.94
149_L320_L1.1140.94
95_N99_Q1.0990.93
293_M296_R1.0970.93
159_C271_T1.0950.93
163_G222_I1.0930.93
238_I242_R1.0930.93
117_A121_S1.0910.93
120_I323_L1.0910.93
159_C190_T1.0910.93
116_I119_Q1.0840.93
169_W173_S1.0840.93
273_M317_A1.0800.93
109_A113_F1.0720.92
241_S246_A1.0700.92
234_P325_Q1.0550.92
66_S70_E1.0510.92
225_L263_T1.0430.91
251_M263_T1.0420.91
220_P275_A1.0390.91
75_V79_F1.0290.91
200_V299_G1.0280.91
106_A137_G1.0270.91
156_L298_I1.0200.90
149_L316_L1.0170.90
99_Q104_M1.0070.90
141_A210_G0.9950.89
144_Q249_R0.9860.89
171_A227_I0.9850.89
117_A126_G0.9810.89
176_A179_I0.9750.88
295_L299_G0.9730.88
102_L143_S0.9650.88
229_G263_T0.9650.88
145_A316_L0.9640.88
163_G250_Q0.9620.88
117_A125_M0.9560.87
89_P92_Y0.9470.87
246_A254_K0.9470.87
182_P289_G0.9450.87
174_P178_K0.9430.87
327_V330_D0.9390.86
150_A207_N0.9390.86
96_G99_Q0.9380.86
163_G283_S0.9320.86
294_V309_G0.9310.86
63_P66_S0.9250.85
68_V71_G0.9190.85
125_M130_L0.9100.85
115_L118_W0.9070.84
134_I138_A0.9010.84
96_G100_L0.8990.84
162_I166_L0.8970.84
146_M150_A0.8970.84
141_A144_Q0.8940.83
202_L307_T0.8870.83
110_I133_L0.8850.83
141_A312_G0.8820.83
236_D244_F0.8790.82
61_L308_V0.8770.82
148_T279_V0.8760.82
117_A129_T0.8760.82
158_F161_V0.8750.82
121_S124_G0.8750.82
122_G304_G0.8740.82
120_I327_V0.8710.82
76_V201_M0.8680.82
226_T267_G0.8650.81
205_I284_M0.8630.81
151_L277_S0.8630.81
90_V211_V0.8630.81
274_L277_S0.8560.81
272_L320_L0.8470.80
163_G223_I0.8460.80
151_L192_A0.8420.80
88_V92_Y0.8410.80
89_P93_I0.8380.79
295_L309_G0.8330.79
71_G75_V0.8280.78
169_W179_I0.8240.78
257_L264_I0.8230.78
128_A131_V0.8110.77
70_E92_Y0.8090.77
80_R84_Q0.8040.76
73_D77_T0.8010.76
67_W70_E0.7960.76
88_V91_D0.7960.76
188_Q270_Q0.7900.75
113_F319_I0.7890.75
85_G88_V0.7870.75
221_P267_G0.7840.75
69_T76_V0.7820.74
236_D241_S0.7760.74
167_G291_G0.7750.74
168_I252_L0.7720.73
270_Q321_D0.7720.73
82_V85_G0.7690.73
297_G301_L0.7650.73
81_P95_N0.7600.72
111_I115_L0.7590.72
130_L161_V0.7580.72
164_L257_L0.7580.72
218_A250_Q0.7560.72
113_F116_I0.7560.72
207_N288_G0.7550.72
263_T275_A0.7550.72
174_P177_A0.7540.72
221_P269_N0.7520.72
90_V94_L0.7520.72
71_G78_H0.7520.72
309_G313_I0.7500.71
171_A230_I0.7480.71
285_I325_Q0.7470.71
82_V89_P0.7460.71
70_E93_I0.7450.71
144_Q309_G0.7420.70
125_M129_T0.7410.70
99_Q102_L0.7400.70
91_D98_Q0.7390.70
87_R204_G0.7390.70
240_A255_V0.7270.69
237_L259_L0.7230.69
87_R308_V0.7210.68
141_A293_M0.7200.68
81_P84_Q0.7170.68
216_I243_S0.7160.68
102_L147_V0.7150.68
189_T194_V0.7150.68
280_V321_D0.7150.68
167_G230_I0.7140.68
293_M305_L0.7140.68
228_L317_A0.7110.67
92_Y95_N0.7040.66
131_V134_I0.7040.66
250_Q254_K0.7010.66
94_L211_V0.7010.66
116_I126_G0.6980.66
119_Q122_G0.6960.66
299_G303_M0.6950.65
198_P278_M0.6950.65
125_M241_S0.6930.65
317_A321_D0.6910.65
110_I134_I0.6880.65
177_A231_N0.6880.65
300_R303_M0.6870.65
62_I65_D0.6840.64
225_L280_V0.6820.64
157_L329_R0.6800.64
240_A259_L0.6800.64
211_V285_I0.6780.64
84_Q87_R0.6760.63
177_A180_I0.6750.63
188_Q321_D0.6680.62
169_W186_A0.6680.62
162_I219_L0.6670.62
88_V323_L0.6510.60
64_L68_V0.6500.60
142_W285_I0.6490.60
96_G158_F0.6490.60
315_I318_I0.6480.60
101_L153_L0.6460.60
184_L195_Y0.6450.60
120_I326_A0.6440.60
168_I228_L0.6440.60
143_S147_V0.6440.60
80_R216_I0.6430.59
170_L177_A0.6420.59
188_Q198_P0.6400.59
92_Y97_F0.6390.59
265_M268_V0.6360.59
293_M300_R0.6360.59
234_P237_L0.6350.59
116_I175_R0.6350.59
181_R227_I0.6320.58
219_L223_I0.6280.58
72_I323_L0.6260.57
187_M219_L0.6240.57
167_G171_A0.6180.56
62_I67_W0.6160.56
136_I210_G0.6090.55
115_L327_V0.6090.55
68_V125_M0.6070.55
70_E82_V0.6070.55
103_G109_A0.6060.55
196_L270_Q0.6050.55
109_A329_R0.6050.55
116_I131_V0.6040.55
120_I219_L0.6030.55
281_I302_D0.6030.55
225_L266_A0.6010.54
145_A320_L0.6000.54
246_A251_M0.5990.54
225_L288_G0.5980.54
93_I96_G0.5980.54
292_Q296_R0.5970.54
74_W79_F0.5960.54
158_F327_V0.5960.54
68_V76_V0.5910.53
127_V130_L0.5910.53
168_I244_F0.5890.53
279_V282_A0.5880.53
244_F289_G0.5870.53
93_I174_P0.5870.53
94_L292_Q0.5870.53
184_L220_P0.5850.52
301_L326_A0.5840.52
104_M157_L0.5830.52
110_I199_I0.5820.52
225_L274_L0.5780.52
293_M297_G0.5780.52
276_L282_A0.5730.51
112_V116_I0.5730.51
145_A212_V0.5720.51
156_L217_F0.5710.51
194_V284_M0.5710.51
87_R292_Q0.5700.51
73_D85_G0.5690.50
217_F310_G0.5660.50
131_V329_R0.5660.50
267_G322_R0.5660.50
248_P251_M0.5640.50
257_L261_M0.5600.49
212_V308_V0.5600.49
267_G289_G0.5590.49
169_W192_A0.5590.49
165_P168_I0.5590.49
66_S236_D0.5560.49
77_T92_Y0.5540.49
118_W156_L0.5540.49
126_G130_L0.5540.49
136_I140_G0.5540.49
283_S293_M0.5530.48
70_E174_P0.5520.48
130_L329_R0.5510.48
223_I316_L0.5500.48
243_S250_Q0.5490.48
187_M285_I0.5450.48
135_A138_A0.5450.48
124_G131_V0.5410.47
76_V156_L0.5410.47
185_D270_Q0.5400.47
65_D72_I0.5400.47
79_F91_D0.5400.47
186_A299_G0.5390.47
306_A310_G0.5380.47
153_L315_I0.5370.47
220_P250_Q0.5360.46
135_A298_I0.5350.46
194_V294_V0.5350.46
187_M209_P0.5350.46
103_G241_S0.5340.46
200_V293_M0.5340.46
80_R299_G0.5340.46
70_E127_V0.5340.46
118_W125_M0.5330.46
70_E104_M0.5330.46
106_A111_I0.5310.46
270_Q273_M0.5280.45
228_L266_A0.5260.45
195_Y260_A0.5260.45
165_P289_G0.5250.45
188_Q269_N0.5240.45
113_F123_V0.5240.45
117_A146_M0.5230.45
208_V284_M0.5230.45
207_N292_Q0.5230.45
255_V328_G0.5230.45
84_Q88_V0.5210.45
217_F274_L0.5210.45
120_I180_I0.5200.44
107_P114_A0.5200.44
98_Q150_A0.5200.44
199_I287_V0.5200.44
178_K181_R0.5200.44
236_D286_A0.5170.44
103_G153_L0.5170.44
120_I125_M0.5160.44
269_N278_M0.5160.44
292_Q299_G0.5150.44
198_P310_G0.5150.44
100_L178_K0.5140.44
135_A315_I0.5140.44
149_L174_P0.5140.44
144_Q147_V0.5110.43
70_E166_L0.5110.43
270_Q278_M0.5100.43
141_A305_L0.5080.43
186_A311_V0.5080.43
166_L219_L0.5070.43
181_R251_M0.5070.43
201_M285_I0.5060.43
195_Y279_V0.5060.43
214_T308_V0.5050.43
98_Q154_T0.5050.43
62_I66_S0.5040.43
206_G239_E0.5040.43
229_G267_G0.5030.42
88_V179_I0.5030.42
185_D191_P0.5020.42
269_N321_D0.5010.42
199_I225_L0.5010.42
182_P322_R0.5000.42
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2onkC 2 0.6582 100 0.557 Contact Map
3d31C 2 0.6469 100 0.565 Contact Map
4tquM 1 0.6921 100 0.593 Contact Map
3rlfF 1 0.8898 100 0.609 Contact Map
3tuiA 2 0.5678 100 0.618 Contact Map
4ymuD 2 0.5791 100 0.618 Contact Map
4tquN 1 0.6893 100 0.633 Contact Map
3rlfG 1 0.6836 100 0.633 Contact Map
2kogA 1 0.3136 32.5 0.955 Contact Map
4mrsA 2 0.3051 27 0.957 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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