GREMLIN Database
NHAA - Na(+)/H(+) antiporter NhaA
UniProt: P13738 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10652
Length: 388 (375)
Sequences: 2141 (1468)
Seq/√Len: 75.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
105_M166_G6.5461.00
112_Y350_F4.2401.00
116_N291_G3.9621.00
58_N61_L3.8411.00
301_P329_M3.7791.00
304_I335_C3.4571.00
191_I232_I3.3441.00
113_L123_R3.3261.00
305_S329_M3.0301.00
346_A361_A2.9701.00
130_A170_I2.9181.00
333_I379_W2.9051.00
190_A215_L2.8881.00
333_I375_I2.8831.00
180_S183_S2.8571.00
298_I302_L2.8421.00
186_V218_A2.8391.00
111_L294_A2.7081.00
330_V379_W2.6811.00
293_I371_S2.6641.00
99_I311_A2.6611.00
24_L269_F2.5551.00
63_I270_A2.5261.00
309_W313_R2.5041.00
190_A211_V2.4911.00
42_L270_A2.4241.00
297_L375_I2.3711.00
151_A323_T2.3201.00
45_P56_N2.2351.00
194_L233_V2.2161.00
195_A236_F2.2111.00
28_M37_W2.2081.00
28_M269_F2.1831.00
181_M185_G2.1781.00
191_I195_A2.1741.00
16_I20_I2.1711.00
187_A219_V2.1671.00
191_I215_L2.1551.00
115_F294_A2.1521.00
105_M170_I2.1511.00
183_S218_A2.1461.00
31_S34_T2.1271.00
190_A214_V2.1151.00
193_V211_V2.0581.00
294_A298_I2.0361.00
361_A365_I2.0151.00
262_L266_L1.9901.00
197_L211_V1.9861.00
81_R252_E1.9801.00
28_M33_A1.9781.00
110_L114_A1.9741.00
377_Y381_R1.9201.00
138_L157_M1.9141.00
197_L207_V1.9071.00
112_Y288_L1.9031.00
99_I307_F1.8941.00
64_N271_N1.8671.00
169_I173_L1.8661.00
158_A162_I1.8141.00
14_G260_A1.7911.00
196_V200_C1.7771.00
305_S332_G1.7381.00
40_D43_E1.7281.00
217_T221_K1.7081.00
297_L372_S1.6781.00
301_P306_L1.6601.00
139_G143_L1.6601.00
307_F311_A1.6151.00
90_S158_A1.6061.00
309_W325_Y1.5980.99
352_S356_E1.5710.99
140_V337_I1.5690.99
190_A218_A1.5670.99
46_V50_V1.5570.99
41_F59_M1.5520.99
13_S261_Y1.5440.99
297_L301_P1.5380.99
286_S360_W1.5350.99
77_L251_L1.5280.99
186_V190_A1.5270.99
312_L328_I1.5200.99
103_G307_F1.5170.99
289_P364_G1.5150.99
43_E60_L1.5060.99
301_P332_G1.5050.99
42_L60_L1.5040.99
212_G233_V1.4960.99
297_L371_S1.4850.99
73_L205_T1.4710.99
258_W262_L1.4660.99
185_G189_V1.4650.99
126_W161_I1.4380.99
127_A130_A1.4220.99
125_G350_F1.4160.99
22_A26_M1.4120.99
20_I24_L1.4110.99
73_L212_G1.4030.99
39_H43_E1.3780.98
144_L377_Y1.3760.98
351_G355_P1.3730.98
352_S355_P1.3730.98
5_H9_S1.3720.98
155_F159_L1.3680.98
301_P305_S1.3580.98
100_A308_C1.3570.98
65_D225_H1.3560.98
26_M366_L1.3450.98
106_I110_L1.3450.98
127_A170_I1.3360.98
378_S382_V1.3040.98
315_K318_H1.2930.98
77_L234_G1.2820.98
131_A134_I1.2780.98
38_Y273_G1.2760.97
308_C312_L1.2740.97
102_I106_I1.2720.97
45_P61_L1.2680.97
297_L329_M1.2630.97
111_L298_I1.2480.97
25_A272_A1.2470.97
113_L117_Y1.2450.97
228_L232_I1.2410.97
103_G320_P1.2160.97
98_V162_I1.2130.97
330_V380_L1.2100.97
144_L148_V1.2080.97
43_E61_L1.2010.96
189_V193_V1.1990.96
57_K61_L1.1980.96
37_W41_F1.1970.96
183_S186_V1.1940.96
373_A377_Y1.1930.96
39_H275_S1.1870.96
368_G372_S1.1850.96
193_V197_L1.1800.96
35_S40_D1.1790.96
155_F331_V1.1780.96
62_W66_A1.1580.96
312_L325_Y1.1540.95
7_F256_H1.1500.95
357_L360_W1.1380.95
356_E360_W1.1360.95
134_I171_I1.1200.95
57_K221_K1.1140.95
134_I165_L1.1140.95
114_A117_Y1.1110.94
374_V378_S1.1090.94
49_R54_E1.1060.94
110_L113_L1.1060.94
246_S249_K1.0970.94
342_S345_I1.0950.94
364_G368_G1.0950.94
326_Q383_R1.0930.94
73_L247_P1.0910.94
102_I166_G1.0870.94
175_Y345_I1.0870.94
301_P333_I1.0860.94
293_I368_G1.0850.94
144_L330_V1.0800.94
80_K84_M1.0770.93
351_G358_I1.0760.93
152_L331_V1.0720.93
28_M31_S1.0710.93
378_S381_R1.0590.93
312_L319_L1.0480.92
95_A99_I1.0410.92
91_L95_A1.0400.92
167_A341_M1.0370.92
298_I301_P1.0310.92
79_V344_F1.0280.92
360_W364_G1.0200.91
323_T331_V1.0150.91
329_M333_I1.0140.91
256_H260_A1.0130.91
89_A234_G1.0130.91
205_T251_L1.0060.91
194_L237_I0.9940.90
371_S375_I0.9910.90
39_H60_L0.9880.90
129_P134_I0.9870.90
198_N237_I0.9860.90
90_S93_Q0.9770.90
289_P360_W0.9760.90
207_V210_L0.9750.90
103_G159_L0.9690.89
50_V53_L0.9670.89
293_I297_L0.9650.89
144_L147_R0.9600.89
115_F291_G0.9590.89
291_G295_G0.9590.89
194_L215_L0.9510.88
51_G55_I0.9460.88
183_S221_K0.9450.88
31_S35_S0.9390.88
45_P58_N0.9390.88
89_A158_A0.9330.87
215_L233_V0.9190.87
111_L302_L0.9150.86
73_L233_V0.9140.86
288_L291_G0.9110.86
34_T39_H0.9110.86
336_G372_S0.9070.86
27_I31_S0.9060.86
98_V102_I0.9040.86
77_L237_I0.8890.85
28_M41_F0.8870.85
8_F13_S0.8860.84
168_I227_T0.8860.84
346_A358_I0.8850.84
219_V222_S0.8850.84
48_L59_M0.8830.84
42_L59_M0.8800.84
68_M271_N0.8780.84
64_N68_M0.8770.84
289_P293_I0.8760.84
358_I362_K0.8660.83
158_A236_F0.8650.83
94_A98_V0.8630.83
61_L220_L0.8620.83
128_I365_I0.8580.83
191_I236_F0.8570.82
44_T51_G0.8570.82
57_K62_W0.8550.82
168_I231_V0.8540.82
58_N62_W0.8490.82
28_M38_Y0.8440.81
170_I174_F0.8410.81
44_T59_M0.8400.81
11_D260_A0.8390.81
178_D224_V0.8380.81
182_A186_V0.8380.81
13_S16_I0.8350.81
192_A196_V0.8340.81
205_T250_R0.8310.80
232_I236_F0.8300.80
57_K220_L0.8280.80
73_L255_L0.8240.80
319_L325_Y0.8240.80
221_K228_L0.8200.80
301_P343_I0.8180.79
64_N344_F0.8160.79
202_A207_V0.8130.79
306_L310_L0.8100.79
79_V323_T0.8080.79
187_A215_L0.8060.78
190_A193_V0.8060.78
150_L157_M0.8060.78
198_N208_Y0.8010.78
130_A166_G0.8000.78
96_F312_L0.7980.78
194_L211_V0.7970.78
7_F13_S0.7930.77
209_I251_L0.7910.77
43_E58_N0.7870.77
80_K252_E0.7860.77
137_A331_V0.7860.77
75_V138_L0.7850.77
103_G308_C0.7840.76
24_L356_E0.7830.76
48_L58_N0.7810.76
62_W226_A0.7760.76
94_A268_A0.7760.76
269_F279_V0.7760.76
127_A175_Y0.7740.76
68_M259_V0.7720.75
81_R264_L0.7710.75
137_A156_L0.7690.75
87_S157_M0.7670.75
176_T224_V0.7660.75
180_S185_G0.7630.75
314_L318_H0.7610.74
79_V90_S0.7600.74
332_G368_G0.7600.74
281_L285_T0.7570.74
21_A269_F0.7560.74
150_L153_K0.7560.74
137_A226_A0.7560.74
222_S226_A0.7560.74
83_L240_K0.7540.74
74_L259_V0.7530.74
119_D123_R0.7520.73
128_I342_S0.7500.73
127_A279_V0.7490.73
9_S256_H0.7480.73
284_L287_I0.7470.73
148_V153_K0.7440.73
215_L219_V0.7420.72
286_S289_P0.7410.72
10_S14_G0.7410.72
346_A365_I0.7400.72
61_L64_N0.7330.72
207_V211_V0.7320.71
194_L212_G0.7310.71
335_C364_G0.7310.71
87_S330_V0.7290.71
206_G210_L0.7290.71
57_K60_L0.7270.71
379_W383_R0.7270.71
7_F14_G0.7260.71
324_T327_Q0.7250.71
115_F123_R0.7220.70
55_I217_T0.7220.70
45_P51_G0.7200.70
159_L233_V0.7180.70
66_A70_V0.7180.70
145_G153_K0.7180.70
142_A157_M0.7180.70
121_I364_G0.7170.70
329_M332_G0.7170.70
10_S13_S0.7150.70
327_Q383_R0.7150.70
283_G286_S0.7150.70
66_A220_L0.7140.70
311_A317_A0.7110.69
48_L287_I0.7080.69
37_W40_D0.7080.69
219_V226_A0.7050.69
40_D44_T0.7030.68
101_A231_V0.7030.68
237_I368_G0.7030.68
65_D136_F0.7020.68
61_L69_A0.7020.68
139_G142_A0.7020.68
27_I45_P0.7010.68
185_G188_A0.7000.68
308_C317_A0.7000.68
35_S39_H0.6990.68
210_L214_V0.6970.68
101_A369_S0.6950.67
183_S217_T0.6950.67
6_R9_S0.6940.67
172_A227_T0.6940.67
176_T179_L0.6940.67
165_L231_V0.6930.67
87_S93_Q0.6910.67
97_P134_I0.6880.67
11_D256_H0.6870.67
96_F317_A0.6870.67
59_M262_L0.6840.66
79_V157_M0.6840.66
19_I22_A0.6830.66
250_R254_V0.6810.66
293_I367_V0.6800.66
41_F232_I0.6780.66
98_V219_V0.6760.65
168_I364_G0.6760.65
187_A218_A0.6750.65
114_A173_L0.6750.65
149_P380_L0.6740.65
194_L236_F0.6740.65
138_L154_I0.6710.65
230_G336_G0.6690.65
25_A38_Y0.6690.65
208_Y237_I0.6680.64
238_P247_P0.6650.64
252_E256_H0.6640.64
234_G330_V0.6630.64
251_L255_L0.6630.64
112_Y125_G0.6630.64
219_V232_I0.6620.64
116_N123_R0.6600.63
208_Y247_P0.6600.63
271_N344_F0.6570.63
40_D60_L0.6560.63
366_L370_I0.6540.63
23_I26_M0.6540.63
94_A162_I0.6510.62
91_L98_V0.6480.62
147_R381_R0.6480.62
49_R52_S0.6480.62
46_V55_I0.6470.62
74_L97_P0.6460.62
37_W378_S0.6460.62
9_S253_H0.6450.62
161_I369_S0.6450.62
46_V49_R0.6440.62
68_M79_V0.6440.62
311_A315_K0.6410.61
61_L221_K0.6400.61
347_S358_I0.6360.61
369_S373_A0.6360.61
28_M248_A0.6340.60
7_F11_D0.6340.60
274_V363_L0.6330.60
24_L28_M0.6320.60
109_A161_I0.6320.60
103_G311_A0.6310.60
76_G234_G0.6300.60
37_W269_F0.6290.60
276_L282_D0.6290.60
60_L138_L0.6270.60
194_L198_N0.6230.59
140_V298_I0.6230.59
73_L215_L0.6220.59
120_P123_R0.6220.59
154_I263_I0.6210.59
347_S362_K0.6210.59
189_V219_V0.6200.59
213_V217_T0.6200.59
157_M234_G0.6180.58
305_S309_W0.6180.58
197_L202_A0.6170.58
69_A136_F0.6160.58
100_A328_I0.6160.58
49_R53_L0.6130.58
297_L333_I0.6130.58
68_M263_I0.6110.58
152_L323_T0.6110.58
60_L64_N0.6090.57
121_I357_L0.6080.57
95_A311_A0.6080.57
239_L248_A0.6070.57
85_Q249_K0.6050.57
202_A208_Y0.6050.57
139_G263_I0.6050.57
269_F280_T0.6040.57
107_V302_L0.6020.56
177_N195_A0.6020.56
319_L323_T0.6020.56
55_I58_N0.6020.56
183_S334_L0.6010.56
32_G332_G0.6000.56
128_I357_L0.5980.56
343_I361_A0.5980.56
147_R153_K0.5970.56
306_L311_A0.5960.56
190_A237_I0.5950.56
22_A136_F0.5920.55
25_A273_G0.5920.55
203_R349_A0.5910.55
312_L317_A0.5900.55
68_M261_Y0.5890.55
191_I228_L0.5880.55
177_N287_I0.5880.55
38_Y283_G0.5870.55
18_L139_G0.5860.54
17_I347_S0.5850.54
226_A237_I0.5840.54
169_I342_S0.5820.54
39_H209_I0.5810.54
197_L200_C0.5800.54
27_I114_A0.5800.54
121_I124_E0.5750.53
41_F44_T0.5750.53
182_A185_G0.5750.53
20_I196_V0.5730.53
85_Q292_I0.5720.53
111_L115_F0.5720.53
352_S357_L0.5720.53
143_L355_P0.5700.52
127_A368_G0.5690.52
15_G165_L0.5690.52
21_A142_A0.5690.52
75_V261_Y0.5690.52
7_F376_G0.5690.52
316_L344_F0.5670.52
310_L314_L0.5670.52
237_I251_L0.5650.52
130_A149_P0.5650.52
84_M239_L0.5630.52
373_A376_G0.5630.52
185_G351_G0.5620.51
191_I233_V0.5620.51
23_I27_I0.5610.51
327_Q331_V0.5590.51
247_P250_R0.5590.51
20_I33_A0.5580.51
323_T327_Q0.5580.51
311_A316_L0.5570.51
91_L158_A0.5570.51
208_Y271_N0.5570.51
79_V83_L0.5570.51
160_A166_G0.5570.51
70_V74_L0.5560.51
154_I158_A0.5560.51
87_S234_G0.5550.51
12_A25_A0.5540.50
68_M344_F0.5530.50
281_L375_I0.5530.50
198_N341_M0.5510.50
219_V255_L0.5500.50
259_V377_Y0.5490.50
75_V79_V0.5460.49
49_R56_N0.5460.49
151_A334_L0.5450.49
51_G56_N0.5440.49
130_A167_A0.5430.49
91_L304_I0.5420.49
17_I261_Y0.5420.49
258_W261_Y0.5420.49
27_I147_R0.5420.49
66_A77_L0.5410.49
120_P282_D0.5400.49
302_L306_L0.5390.49
32_G173_L0.5380.48
128_I298_I0.5370.48
331_V372_S0.5370.48
79_V87_S0.5360.48
176_T222_S0.5360.48
289_P292_I0.5350.48
109_A167_A0.5350.48
75_V381_R0.5350.48
118_A121_I0.5350.48
137_A140_V0.5350.48
68_M194_L0.5350.48
83_L89_A0.5350.48
161_I248_A0.5340.48
222_S227_T0.5330.48
110_L192_A0.5320.48
117_Y120_P0.5310.48
129_P137_A0.5300.47
228_L325_Y0.5300.47
79_V162_I0.5290.47
226_A380_L0.5290.47
297_L368_G0.5290.47
167_A308_C0.5280.47
103_G158_A0.5270.47
111_L114_A0.5270.47
203_R250_R0.5260.47
233_V236_F0.5240.47
65_D227_T0.5240.47
352_S358_I0.5230.47
48_L51_G0.5230.47
58_N362_K0.5220.46
370_I373_A0.5220.46
189_V192_A0.5210.46
144_L379_W0.5210.46
308_C335_C0.5210.46
66_A345_I0.5210.46
48_L56_N0.5200.46
4_L50_V0.5190.46
92_R103_G0.5180.46
252_E295_G0.5170.46
171_I348_L0.5170.46
103_G154_I0.5170.46
146_S328_I0.5160.46
330_V333_I0.5160.46
50_V56_N0.5150.46
144_L376_G0.5140.45
109_A138_L0.5140.45
85_Q239_L0.5140.45
46_V56_N0.5120.45
341_M344_F0.5120.45
371_S374_V0.5100.45
69_A226_A0.5100.45
140_V377_Y0.5090.45
94_A335_C0.5070.45
157_M257_P0.5070.45
162_I165_L0.5060.44
113_L174_F0.5060.44
45_P220_L0.5050.44
20_I142_A0.5050.44
198_N202_A0.5050.44
316_L320_P0.5040.44
201_G204_R0.5040.44
168_I176_T0.5040.44
202_A226_A0.5030.44
292_I364_G0.5030.44
186_V214_V0.5030.44
75_V134_I0.5020.44
63_I263_I0.5000.44
195_A215_L0.5000.44
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1zcdA 1 0.9665 100 0.28 Contact Map
4czbA 2 0.9278 99.9 0.829 Contact Map
4cz8A 2 0.9149 99.9 0.847 Contact Map
4bwzA 2 0.8943 99.8 0.853 Contact Map
3zuxA 1 0.7629 54.8 0.974 Contact Map
3orgA 2 0.4304 16.5 0.98 Contact Map
4av3A 2 0.2165 10.5 0.982 Contact Map
2kluA 1 0.0902 6.7 0.983 Contact Map
4u9nA 2 0.366 5.4 0.984 Contact Map
4kjrA 3 0.4304 4.5 0.984 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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