GREMLIN Database
BIOB - Biotin synthase
UniProt: P12996 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10118
Length: 346 (306)
Sequences: 5865 (3417)
Seq/√Len: 195.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
114_E141_R4.7841.00
136_E182_D4.2711.00
158_P171_Q4.1181.00
78_V115_Q3.2711.00
121_K148_D3.2151.00
9_L204_G3.0541.00
29_Q33_Q3.0061.00
56_D65_R2.8841.00
31_H252_I2.7791.00
110_M114_E2.7421.00
78_V119_G2.7021.00
77_E80_Q2.6591.00
219_V254_M2.5861.00
260_R282_N2.5721.00
136_E140_Q2.5701.00
81_V116_M2.5541.00
178_E217_E2.5421.00
136_E179_K2.4451.00
13_T200_K2.3341.00
207_L253_M2.3021.00
82_L119_G2.2991.00
111_P141_R2.2951.00
171_Q175_D2.2211.00
175_D179_K2.1681.00
9_L207_L2.1551.00
39_Q311_N2.1081.00
140_Q144_N2.0731.00
15_L249_V1.9701.00
83_E86_R1.9671.00
119_G122_A1.9591.00
199_V242_D1.9521.00
86_R90_A1.9391.00
189_S209_L1.9301.00
154_L205_L1.9041.00
202_R242_D1.8791.00
85_A120_V1.8551.00
143_A183_A1.8321.00
159_E204_G1.8201.00
114_E145_A1.8011.00
27_A252_I1.7881.00
114_E118_Q1.7581.00
12_V253_M1.7451.00
13_T17_E1.7381.00
115_Q118_Q1.7221.00
229_G232_L1.6961.00
137_S140_Q1.6671.00
157_S201_D1.6601.00
8_T11_Q1.6551.00
80_Q83_E1.6441.00
117_V147_L1.6291.00
206_L250_A1.6251.00
82_L86_R1.6231.00
33_Q38_R1.6201.00
38_R311_N1.6061.00
149_Y186_K1.5761.00
274_A307_K1.5691.00
143_A185_I1.5521.00
87_K90_A1.5501.00
178_E182_D1.5471.00
172_E175_D1.5451.00
139_A183_A1.5341.00
229_G233_A1.5211.00
135_S138_Q1.5201.00
303_Q307_K1.5191.00
88_A93_S1.5151.00
299_D303_Q1.5031.00
25_F279_A1.5001.00
9_L13_T1.4851.00
9_L203_A1.4811.00
21_L245_R1.4801.00
113_L142_L1.4791.00
13_T203_A1.4791.00
29_Q32_R1.4701.00
9_L200_K1.4491.00
226_K237_D1.4411.00
30_V34_H1.4331.00
202_R246_T1.4261.00
244_I276_C1.4131.00
134_L142_L1.4121.00
17_E199_V1.4011.00
160_F163_N1.3961.00
169_T172_E1.3851.00
298_E302_L1.3641.00
29_Q278_M1.3611.00
270_E307_K1.3551.00
198_T201_D1.3481.00
261_L276_C1.3431.00
230_T233_A1.3421.00
42_V302_L1.3411.00
259_V281_A1.3351.00
297_E300_K1.3341.00
141_R144_N1.3321.00
178_E212_L1.3311.00
179_K182_D1.3291.00
110_M138_Q1.3201.00
229_G234_D1.3191.00
83_E87_K1.3051.00
7_W252_I1.2971.00
136_E183_A1.2881.00
16_F249_V1.2811.00
170_Y174_L1.2791.00
207_L211_N1.2781.00
302_L306_R1.2781.00
17_E200_K1.2761.00
230_T234_D1.2601.00
261_L281_A1.2591.00
47_S50_T1.2491.00
278_M308_L1.2341.00
26_E30_V1.2141.00
158_P162_G1.2111.00
82_L122_A1.2101.00
240_A272_T1.2061.00
94_T314_Q1.2051.00
193_V246_T1.1951.00
191_G205_L1.1931.00
89_K124_G1.1861.00
111_P114_E1.1831.00
12_V203_A1.1801.00
12_V207_L1.1791.00
20_L23_L1.1751.00
79_E83_E1.1711.00
7_W12_V1.1681.00
140_Q143_A1.1671.00
21_L25_F1.1561.00
74_R104_N1.1491.00
14_E18_K1.1461.00
113_L131_L1.1451.00
193_V243_F1.1371.00
118_Q145_A1.1311.00
206_L253_M1.1211.00
175_D178_E1.1201.00
303_Q306_R1.1141.00
159_E201_D1.1061.00
50_T101_A1.0931.00
6_R214_T1.0891.00
300_K303_Q1.0851.00
140_Q183_A1.0851.00
117_V127_A1.0811.00
174_L208_Q1.0751.00
158_P208_Q1.0631.00
52_A74_R1.0561.00
177_L187_V1.0511.00
269_N275_M1.0481.00
78_V112_Y1.0391.00
152_H173_R1.0331.00
6_R210_A1.0251.00
154_L170_Y1.0171.00
76_M81_V1.0161.00
203_A207_L1.0151.00
78_V82_L1.0151.00
78_V116_M1.0041.00
12_V249_V1.0011.00
201_D204_G1.0011.00
28_Q278_M1.0001.00
84_S87_K1.0001.00
141_R145_A0.9971.00
204_G207_L0.9850.99
231_P234_D0.9820.99
51_G62_Q0.9790.99
239_D242_D0.9760.99
111_P115_Q0.9740.99
247_I276_C0.9680.99
24_L245_R0.9670.99
16_F246_T0.9600.99
208_Q211_N0.9560.99
103_K133_T0.9560.99
98_M116_M0.9520.99
16_F199_V0.9500.99
170_Y208_Q0.9470.99
89_K123_M0.9410.99
110_M141_R0.9350.99
48_I81_V0.9320.99
277_F305_F0.9290.99
32_R310_L0.9290.99
6_R255_P0.9260.99
56_D63_S0.9200.99
51_G70_L0.9190.99
199_V246_T0.9110.99
152_H176_T0.9110.99
87_K91_A0.9100.99
158_P170_Y0.9070.99
38_R310_L0.8990.99
27_A30_V0.8970.99
131_L150_Y0.8960.99
274_A304_L0.8950.99
109_D112_Y0.8950.99
29_Q310_L0.8810.99
31_H34_H0.8760.99
209_L219_V0.8750.99
18_K24_L0.8730.99
5_P11_Q0.8720.99
114_E144_N0.8690.99
116_M119_G0.8620.99
306_R311_N0.8600.99
226_K234_D0.8600.99
73_E80_Q0.8590.99
84_S96_F0.8520.99
39_Q313_Q0.8510.99
244_I279_A0.8480.98
306_R312_P0.8440.98
284_I301_D0.8410.98
63_S231_P0.8390.98
115_Q119_G0.8360.98
104_N133_T0.8340.98
228_K233_A0.8330.98
305_F310_L0.8330.98
161_Y173_R0.8300.98
18_K21_L0.8220.98
181_R187_V0.8210.98
48_I98_M0.8200.98
25_F244_I0.8190.98
42_V305_F0.8180.98
61_P227_V0.8170.98
86_R89_K0.8160.98
118_Q122_A0.8150.98
25_F248_A0.8120.98
174_L178_E0.8100.98
86_R123_M0.8100.98
27_A31_H0.8030.98
32_R36_D0.8010.98
46_L96_F0.8010.98
179_K183_A0.8000.98
19_P245_R0.8000.98
82_L85_A0.7970.98
271_Q274_A0.7910.98
178_E211_N0.7890.98
150_Y180_V0.7880.98
52_A72_A0.7870.98
209_L254_M0.7870.98
207_L210_A0.7860.98
225_V293_T0.7860.98
305_F312_P0.7850.97
18_K26_E0.7850.97
97_C262_S0.7820.97
206_L209_L0.7820.97
69_G229_G0.7770.97
44_T93_S0.7740.97
121_K127_A0.7670.97
107_E141_R0.7660.97
118_Q144_N0.7650.97
221_I250_A0.7650.97
154_L208_Q0.7620.97
262_S285_F0.7590.97
181_R213_P0.7580.97
76_M84_S0.7520.97
104_N107_E0.7510.97
13_T199_V0.7500.97
48_I101_A0.7480.97
74_R108_R0.7470.97
55_E103_K0.7460.97
35_F259_V0.7430.97
240_A269_N0.7400.96
159_E162_G0.7400.96
139_A185_I0.7390.96
55_E167_T0.7380.96
241_F272_T0.7370.96
195_L235_N0.7350.96
21_L241_F0.7340.96
138_Q141_R0.7330.96
227_V230_T0.7310.96
206_L254_M0.7310.96
159_E163_N0.7270.96
79_E119_G0.7240.96
66_Y231_P0.7230.96
214_T255_P0.7230.96
32_R40_V0.7180.96
245_R249_V0.7140.96
247_I281_A0.7100.95
244_I275_M0.7090.95
154_L157_S0.7080.95
298_E314_Q0.7070.95
44_T88_A0.7070.95
171_Q208_Q0.7070.95
276_C281_A0.7070.95
40_V277_F0.7060.95
147_L185_I0.7060.95
14_E17_E0.7030.95
10_S14_E0.7000.95
120_V127_A0.6990.95
162_G165_I0.6980.95
226_K233_A0.6960.95
299_D302_L0.6940.95
35_F255_P0.6930.95
56_D167_T0.6930.95
203_A253_M0.6930.95
85_A125_L0.6900.95
40_V310_L0.6900.95
16_F203_A0.6900.95
10_S200_K0.6890.95
133_T173_R0.6830.94
135_S176_T0.6820.94
110_M142_L0.6810.94
50_T73_E0.6800.94
177_L189_S0.6790.94
25_F29_Q0.6750.94
292_T295_N0.6740.94
126_E148_D0.6740.94
254_M257_S0.6740.94
79_E82_L0.6720.94
11_Q14_E0.6710.94
209_L258_Y0.6680.94
58_K66_Y0.6660.94
89_K94_T0.6650.94
135_S179_K0.6650.94
199_V202_R0.6650.94
108_R112_Y0.6640.94
139_A176_T0.6640.94
82_L123_M0.6600.93
19_P23_L0.6590.93
160_F196_G0.6580.93
268_M272_T0.6580.93
160_F164_I0.6560.93
131_L142_L0.6560.93
160_F195_L0.6550.93
12_V206_L0.6550.93
209_L217_E0.6550.93
285_F290_L0.6550.93
223_M243_F0.6510.93
40_V305_F0.6510.93
135_S172_E0.6490.93
64_S71_E0.6470.93
7_W253_M0.6450.93
89_K125_L0.6440.93
265_R276_C0.6360.92
129_M147_L0.6360.92
177_L181_R0.6360.92
59_Y173_R0.6340.92
96_F127_A0.6330.92
203_A249_V0.6320.92
43_S222_N0.6320.92
284_I305_F0.6310.92
30_V33_Q0.6300.92
263_A281_A0.6270.92
35_F256_T0.6260.91
180_V185_I0.6260.91
163_N196_G0.6250.91
113_L117_V0.6240.91
83_E90_A0.6230.91
270_E300_K0.6210.91
54_P105_P0.6200.91
157_S204_G0.6190.91
23_L26_E0.6180.91
107_E138_Q0.6180.91
47_S264_G0.6150.91
134_L137_S0.6130.91
49_K100_A0.6120.91
192_I195_L0.6120.91
18_K245_R0.6120.91
36_D282_N0.6110.90
164_I232_L0.6100.90
240_A275_M0.6100.90
21_L38_R0.6100.90
188_C222_N0.6090.90
41_Q313_Q0.6080.90
47_S262_S0.6070.90
133_T152_H0.6060.90
156_T160_F0.6050.90
240_A271_Q0.6050.90
134_L138_Q0.6050.90
42_V312_P0.6020.90
213_P257_S0.6000.90
48_I131_L0.6000.90
274_A308_L0.6000.90
238_V243_F0.6000.90
304_L307_K0.5950.89
114_E142_L0.5940.89
95_R128_C0.5940.89
91_A299_D0.5930.89
163_N166_T0.5890.89
22_D25_F0.5890.89
247_I280_G0.5890.89
193_V202_R0.5890.89
44_T314_Q0.5890.89
239_D243_F0.5880.89
305_F308_L0.5850.88
180_V183_A0.5850.88
139_A150_Y0.5840.88
258_Y282_N0.5810.88
43_S153_N0.5810.88
110_M129_M0.5800.88
193_V238_V0.5790.88
23_L27_A0.5790.88
49_K288_C0.5780.88
61_P289_K0.5780.88
220_P223_M0.5780.88
251_R282_N0.5770.88
22_D26_E0.5750.88
56_D64_S0.5750.88
241_F245_R0.5740.88
188_C262_S0.5730.87
242_D246_T0.5720.87
47_S192_I0.5690.87
139_A180_V0.5690.87
95_R260_R0.5690.87
263_A293_T0.5680.87
97_C151_N0.5660.87
98_M120_V0.5640.87
112_Y116_M0.5610.86
271_Q275_M0.5600.86
9_L12_V0.5580.86
270_E304_L0.5570.86
174_L212_L0.5560.86
192_I264_G0.5550.86
273_Q284_I0.5550.86
42_V298_E0.5550.86
188_C264_G0.5530.86
272_T276_C0.5510.85
126_E149_Y0.5500.85
52_A104_N0.5500.85
161_Y165_I0.5490.85
181_R220_P0.5460.85
250_A254_M0.5450.85
21_L24_L0.5450.85
7_W11_Q0.5430.85
78_V81_V0.5400.84
48_I100_A0.5400.84
290_L294_P0.5390.84
110_M131_L0.5370.84
191_G221_I0.5370.84
266_E296_P0.5370.84
210_A214_T0.5360.84
82_L96_F0.5340.84
113_L116_M0.5340.84
180_V187_V0.5340.84
47_S73_E0.5330.83
154_L212_L0.5330.83
296_P300_K0.5320.83
61_P165_I0.5320.83
197_E202_R0.5310.83
273_Q285_F0.5310.83
219_V257_S0.5300.83
263_A273_Q0.5300.83
6_R207_L0.5300.83
212_L218_S0.5290.83
45_L95_R0.5280.83
62_Q97_C0.5270.83
189_S258_Y0.5270.83
26_E29_Q0.5270.83
186_K217_E0.5260.83
160_F191_G0.5240.82
51_G61_P0.5230.82
249_V253_M0.5220.82
272_T275_M0.5200.82
181_R217_E0.5190.82
12_V15_L0.5190.82
159_E198_T0.5180.82
84_S88_A0.5170.82
177_L212_L0.5160.81
59_Y262_S0.5130.81
66_Y107_E0.5120.81
85_A123_M0.5110.81
170_Y201_D0.5090.81
9_L253_M0.5090.81
112_Y115_Q0.5080.80
56_D166_T0.5070.80
199_V249_V0.5030.80
29_Q38_R0.5010.80
41_Q188_C0.5010.80
222_N264_G0.5000.79
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1r30A 2 0.8988 100 0.38 Contact Map
3t7vA 1 0.9046 100 0.448 Contact Map
3iixA 1 0.9133 100 0.46 Contact Map
4r33A 2 0.9133 100 0.466 Contact Map
4wcxA 1 0.9769 100 0.49 Contact Map
4rtbA 2 0.9249 100 0.505 Contact Map
4u0oB 1 0.7081 100 0.683 Contact Map
1oltA 1 0.922 99.8 0.784 Contact Map
4jc0A 1 0.8931 99.8 0.79 Contact Map
2qgqA 1 0.7139 99.8 0.793 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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