GREMLIN Database
IAP - Alkaline phosphatase isozyme conversion protein
UniProt: P10423 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10488
Length: 345 (275)
Sequences: 6258 (4800)
Seq/√Len: 289.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
64_Q67_Q4.0541.00
62_R66_Q3.7981.00
65_F171_A3.5181.00
108_A164_E3.3731.00
150_V206_L2.9611.00
111_Q166_G2.7501.00
153_E156_E2.6211.00
54_E173_S2.5461.00
52_P56_L2.4591.00
148_L171_A2.4351.00
107_K164_E2.3661.00
115_M200_V2.3611.00
36_Q60_Y2.3281.00
184_N188_R2.2991.00
106_G166_G2.2561.00
61_I118_L2.2431.00
55_M59_D2.2331.00
58_A100_V2.2261.00
103_A168_R2.2191.00
110_Q165_Y2.2141.00
151_M171_A2.2111.00
115_M172_T2.2001.00
182_A265_I2.1861.00
186_L269_A2.1831.00
34_N38_R2.1591.00
39_H44_F2.0221.00
38_R293_A1.9981.00
285_N290_Q1.9581.00
61_I171_A1.9281.00
77_F81_Y1.9261.00
111_Q168_R1.9191.00
62_R74_I1.8761.00
71_R105_E1.8591.00
58_A98_S1.8571.00
36_Q64_Q1.8241.00
59_D63_Q1.8061.00
37_A149_G1.7891.00
292_R295_T1.7751.00
37_A145_A1.7541.00
48_M51_T1.7391.00
63_Q67_Q1.7301.00
42_T139_Q1.7191.00
54_E118_L1.7061.00
32_F153_E1.6931.00
101_I170_V1.6791.00
103_A166_G1.6731.00
45_P53_A1.6361.00
29_P32_F1.5991.00
172_T200_V1.5841.00
116_A148_L1.5831.00
212_L215_N1.5481.00
303_S309_L1.5471.00
18_F21_P1.5061.00
17_C21_P1.5031.00
114_I151_M1.4891.00
97_G173_S1.4871.00
65_F155_A1.4851.00
59_D62_R1.4831.00
104_H169_F1.4831.00
109_P196_N1.4821.00
58_A118_L1.4741.00
36_Q68_M1.4691.00
32_F156_E1.4611.00
53_A56_L1.4601.00
64_Q156_E1.4521.00
155_A169_F1.4261.00
158_L167_I1.4191.00
110_Q198_L1.4141.00
168_R185_L1.4001.00
64_Q68_M1.3791.00
302_N306_D1.3701.00
41_A293_A1.3561.00
170_V185_L1.3561.00
76_T96_T1.3541.00
144_N202_N1.3541.00
65_F102_A1.3481.00
62_R100_V1.3231.00
194_K271_I1.3221.00
163_T167_I1.3071.00
48_M177_E1.3011.00
63_Q66_Q1.2831.00
143_D175_E1.2811.00
183_E187_K1.2661.00
116_A151_M1.2651.00
36_Q152_L1.2601.00
70_Y169_F1.2601.00
34_N293_A1.2581.00
103_A111_Q1.2571.00
39_H43_F1.2461.00
293_A298_F1.2301.00
34_N298_F1.2111.00
68_M159_K1.2101.00
139_Q292_R1.2091.00
52_P55_M1.2071.00
57_S118_L1.1931.00
212_L218_V1.1901.00
186_L265_I1.1821.00
120_T138_L1.1731.00
20_L23_H1.1711.00
121_Y138_L1.1691.00
113_I200_V1.1681.00
75_R189_M1.1651.00
174_G180_L1.1611.00
49_T174_G1.1521.00
74_I98_S1.1291.00
215_N218_V1.1251.00
55_M98_S1.1181.00
35_T39_H1.1111.00
97_G174_G1.1081.00
143_D287_D1.1001.00
185_L197_T1.0991.00
186_L266_F1.0951.00
231_R234_A1.0881.00
215_N219_K1.0801.00
104_H163_T1.0801.00
142_D205_N1.0771.00
193_E196_N1.0761.00
194_K269_A1.0741.00
17_C22_V1.0711.00
40_I57_S1.0701.00
113_I197_T1.0681.00
113_I170_V1.0641.00
77_F80_R1.0641.00
233_L237_R1.0541.00
70_Y104_H1.0521.00
15_G18_F1.0521.00
151_M169_F1.0511.00
156_E160_N1.0431.00
200_V266_F1.0321.00
189_M193_E1.0301.00
212_L217_G1.0281.00
41_A141_M1.0241.00
19_I23_H1.0241.00
107_K162_P1.0171.00
172_T182_A1.0171.00
116_A144_N1.0141.00
267_D273_V1.0131.00
106_G165_Y1.0121.00
187_K269_A1.0121.00
36_Q39_H1.0061.00
36_Q153_E1.0051.00
115_M170_V1.0041.00
16_V19_I1.0021.00
230_D233_L1.0001.00
216_S219_K0.9981.00
263_A275_S0.9971.00
78_N81_Y0.9961.00
39_H64_Q0.9941.00
106_G163_T0.9941.00
301_G305_H0.9941.00
213_Y216_S0.9931.00
39_H56_L0.9921.00
117_H262_D0.9891.00
75_R79_S0.9831.00
70_Y102_A0.9821.00
78_N96_T0.9811.00
60_Y64_Q0.9771.00
30_G34_N0.9761.00
11_A14_L0.9631.00
207_I210_D0.9631.00
172_T266_F0.9471.00
114_I167_I0.9471.00
158_L163_T0.9471.00
73_D103_A0.9471.00
212_L216_S0.9391.00
175_E287_D0.9381.00
75_R184_N0.9361.00
287_D291_Q0.9351.00
235_I238_S0.9341.00
117_H143_D0.9311.00
153_E157_R0.9301.00
118_L171_A0.9281.00
183_E265_I0.9261.00
28_K157_R0.9251.00
35_T38_R0.9201.00
142_D208_V0.9171.00
10_A13_A0.9161.00
31_D35_T0.9151.00
47_R145_A0.9071.00
75_R188_R0.9061.00
244_T247_P0.9051.00
296_P301_G0.9051.00
229_R233_L0.9011.00
17_C20_L0.9001.00
114_I169_F0.8951.00
36_Q149_G0.8941.00
174_G177_E0.8901.00
175_E286_K0.8851.00
38_R43_F0.8821.00
117_H175_E0.8791.00
104_H159_K0.8771.00
12_L15_G0.8701.00
65_F152_L0.8691.00
33_A305_H0.8681.00
77_F82_I0.8661.00
120_T140_G0.8651.00
75_R99_T0.8631.00
70_Y155_A0.8621.00
62_R72_S0.8621.00
264_E268_K0.8601.00
39_H60_Y0.8591.00
76_T79_S0.8571.00
49_T97_G0.8571.00
232_A236_A0.8541.00
21_P24_A0.8401.00
213_Y218_V0.8371.00
19_I22_V0.8371.00
78_N94_N0.8361.00
243_A247_P0.8331.00
120_T145_A0.8311.00
138_L289_Y0.8281.00
49_T177_E0.8201.00
69_G72_S0.8191.00
13_A17_C0.8151.00
36_Q156_E0.8131.00
234_A237_R0.8131.00
281_W284_G0.8050.99
298_F302_N0.7990.99
79_S83_Y0.7990.99
203_L308_R0.7930.99
105_E108_A0.7930.99
56_L59_D0.7900.99
49_T54_E0.7890.99
101_I168_R0.7890.99
13_A16_V0.7870.99
10_A14_L0.7860.99
47_R57_S0.7830.99
301_G304_W0.7780.99
303_S306_D0.7780.99
68_M152_L0.7730.99
55_M58_A0.7690.99
227_L231_R0.7660.99
30_G299_P0.7610.99
232_A235_I0.7560.99
58_A62_R0.7550.99
214_F217_G0.7540.99
45_P52_P0.7530.99
199_L228_T0.7500.99
231_R235_I0.7470.99
310_D314_H0.7460.99
13_A18_F0.7440.99
230_D234_A0.7430.99
58_A74_I0.7380.99
71_R103_A0.7310.99
18_F22_V0.7310.99
142_D296_P0.7290.99
76_T98_S0.7270.99
286_K291_Q0.7240.99
260_C264_E0.7230.99
77_F173_S0.7220.99
241_I245_T0.7210.99
265_I268_K0.7210.99
185_L266_F0.7180.99
30_G33_A0.7170.99
308_R312_H0.7150.99
145_A149_G0.7130.99
17_C24_A0.7130.99
197_T271_I0.7120.99
200_V273_V0.7120.99
146_A296_P0.7090.99
61_I148_L0.7050.99
158_L161_T0.7030.99
309_L313_Q0.7020.99
65_F169_F0.7010.99
242_A247_P0.6990.99
17_C23_H0.6940.98
46_G137_T0.6930.98
142_D146_A0.6920.98
190_S193_E0.6920.98
54_E97_G0.6900.98
117_H176_E0.6900.98
257_T273_V0.6860.98
183_E268_K0.6850.98
104_H158_L0.6840.98
28_K33_A0.6820.98
9_T15_G0.6760.98
40_I148_L0.6740.98
143_D262_D0.6700.98
176_E287_D0.6700.98
165_Y198_L0.6690.98
79_S82_I0.6680.98
74_I77_F0.6680.98
144_N204_D0.6670.98
111_Q197_T0.6670.98
32_F35_T0.6650.98
12_L19_I0.6640.98
75_R78_N0.6640.98
285_N288_G0.6640.98
39_H63_Q0.6570.98
65_F70_Y0.6550.98
111_Q196_N0.6480.98
189_M194_K0.6460.98
184_N187_K0.6460.98
175_E202_N0.6460.98
37_A40_I0.6460.98
15_G26_S0.6450.98
288_G291_Q0.6430.98
154_L158_L0.6420.97
16_V20_L0.6400.97
73_D101_I0.6390.97
14_L20_L0.6390.97
54_E61_I0.6380.97
215_N275_S0.6380.97
188_R193_E0.6370.97
117_H287_D0.6370.97
48_M53_A0.6360.97
41_A139_Q0.6360.97
112_I198_L0.6350.97
40_I145_A0.6320.97
213_Y219_K0.6290.97
30_G302_N0.6250.97
182_A262_D0.6240.97
176_E179_K0.6230.97
80_R94_N0.6230.97
143_D176_E0.6220.97
74_I79_S0.6210.97
190_S194_K0.6200.97
33_A149_G0.6190.97
214_F218_V0.6180.97
208_V283_L0.6160.97
147_G204_D0.6160.97
183_E269_A0.6150.97
110_Q166_G0.6150.97
214_F219_K0.6130.97
23_H27_P0.6130.97
76_T81_Y0.6100.97
309_L312_H0.6090.97
175_E204_D0.6070.97
32_F36_Q0.6070.97
14_L19_I0.6070.97
285_N295_T0.6070.97
176_E262_D0.6000.96
229_R232_A0.5980.96
61_I169_F0.5960.96
151_M203_L0.5960.96
236_A243_A0.5960.96
235_I239_H0.5960.96
150_V305_H0.5940.96
170_V266_F0.5940.96
31_D34_N0.5930.96
175_E261_N0.5930.96
116_A147_G0.5930.96
80_R83_Y0.5920.96
238_S242_A0.5920.96
14_L23_H0.5920.96
16_V24_A0.5910.96
185_L189_M0.5900.96
46_G120_T0.5870.96
261_N286_K0.5870.96
15_G19_I0.5860.96
37_A301_G0.5820.96
240_G245_T0.5800.95
107_K163_T0.5800.95
300_A304_W0.5790.95
19_I24_A0.5760.95
142_D278_A0.5750.95
101_I190_S0.5740.95
179_K182_A0.5740.95
46_G122_A0.5740.95
16_V23_H0.5730.95
100_V171_A0.5690.95
232_A274_L0.5690.95
12_L16_V0.5670.95
146_A205_N0.5660.95
143_D286_K0.5640.95
14_L22_V0.5610.95
13_A21_P0.5600.95
185_L192_T0.5590.95
45_P56_L0.5590.95
295_T298_F0.5530.94
14_L17_C0.5510.94
58_A173_S0.5510.94
81_Y97_G0.5500.94
306_D309_L0.5450.94
240_G243_A0.5430.94
28_K305_H0.5380.93
109_P166_G0.5350.93
190_S197_T0.5350.93
68_M102_A0.5340.93
32_F64_Q0.5330.93
62_R78_N0.5330.93
150_V154_L0.5330.93
18_F24_A0.5310.93
16_V21_P0.5290.93
213_Y217_G0.5290.93
110_Q196_N0.5260.93
90_K93_H0.5240.92
22_V25_S0.5240.92
118_L148_L0.5230.92
244_T291_Q0.5220.92
113_I168_R0.5210.92
9_T12_L0.5210.92
33_A37_A0.5190.92
57_S61_I0.5180.92
20_L24_A0.5180.92
19_I25_S0.5160.92
60_Y152_L0.5130.92
108_A166_G0.5130.92
236_A239_H0.5110.91
159_K162_P0.5110.91
207_I296_P0.5110.91
180_L184_N0.5100.91
116_A171_A0.5070.91
262_D287_D0.5050.91
182_A263_A0.5050.91
226_K230_D0.5050.91
243_A246_N0.5050.91
117_H204_D0.5040.91
37_A41_A0.5020.91
113_I172_T0.5010.91
237_R243_A0.5010.91
29_P157_R0.5000.91
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2ek8A 2 0.8174 100 0.432 Contact Map
4tweA 2 0.8841 100 0.47 Contact Map
3iibA 2 0.8116 100 0.474 Contact Map
3kasA 2 0.8928 100 0.475 Contact Map
4mhpA 1 0.8261 100 0.48 Contact Map
1tkjA 1 0.7275 100 0.482 Contact Map
3fedA 2 0.8696 100 0.483 Contact Map
3pb6X 1 0.8203 100 0.485 Contact Map
2afwA 5 0.829 100 0.486 Contact Map
3guxA 1 0.6986 100 0.487 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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