GREMLIN Database
MEPA - Penicillin-insensitive murein endopeptidase
UniProt: P0C0T5 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10580
Length: 274 (257)
Sequences: 450 (286)
Seq/√Len: 17.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
45_I48_A6.2371.00
75_V98_M5.4921.00
74_L98_M5.1111.00
164_I200_V5.0531.00
70_G74_L4.9591.00
78_I119_V4.9021.00
36_S220_S4.3661.00
45_I71_H4.1171.00
194_R233_D3.6451.00
128_T134_Q3.5771.00
74_L119_V3.2900.99
64_D136_L3.0800.99
185_Q189_D3.0580.99
142_D207_R3.0090.99
57_H82_S2.8950.99
121_I212_V2.6580.97
80_R172_D2.5760.97
238_E241_S2.5070.96
179_N207_R2.4990.96
241_S244_E2.4890.96
77_F214_L2.4610.96
39_S203_W2.3050.94
22_P269_L2.1530.91
253_P256_K2.0040.88
125_L128_T1.9980.88
157_K160_I1.9870.88
123_L164_I1.9470.87
22_P258_P1.8380.83
105_R261_L1.8190.82
176_I203_W1.8060.82
140_A208_A1.7710.81
23_W58_Y1.7610.80
99_G106_F1.7490.80
170_D212_V1.7320.79
100_M106_F1.7090.78
164_I210_M1.7010.78
74_L78_I1.6720.77
236_G241_S1.6630.76
36_S45_I1.6520.76
247_K253_P1.5960.73
164_I178_V1.5870.73
19_A61_M1.5790.72
133_A137_R1.5270.70
145_S148_G1.5180.69
77_F163_L1.4970.68
77_F170_D1.4710.67
240_Q244_E1.4650.66
246_P251_T1.4380.65
174_T229_P1.4290.64
124_Q140_A1.4280.64
236_G239_L1.4190.64
142_D190_A1.3990.63
247_K255_K1.3910.62
49_D79_Q1.3720.61
70_G75_V1.3640.61
53_I68_Y1.3420.59
161_F189_D1.3380.59
238_E242_W1.3330.59
105_R135_L1.3320.59
143_L160_I1.3200.58
161_F190_A1.2900.56
255_K258_P1.2810.56
58_Y69_F1.2780.56
29_P50_T1.2770.56
32_G46_V1.2650.55
179_N182_I1.2600.55
77_F212_V1.2560.54
144_V181_A1.2410.53
66_R89_G1.2330.53
34_A269_L1.2300.53
31_P35_Q1.2260.52
63_T135_L1.2250.52
117_L121_I1.2170.52
93_V134_Q1.2060.51
22_P268_L1.2040.51
91_G125_L1.1980.51
251_T256_K1.1960.51
20_A23_W1.1950.51
207_R229_P1.1950.51
241_S245_P1.1830.50
174_T225_D1.1640.49
174_T215_R1.1600.48
55_S65_Q1.1570.48
37_I99_G1.1490.48
88_L163_L1.1320.47
70_G98_M1.1310.47
53_I60_V1.1310.47
153_S237_A1.1260.46
200_V210_M1.1200.46
23_W92_T1.1020.45
115_T252_K1.0990.45
42_N105_R1.0960.45
239_L242_W1.0950.44
36_S217_P1.0850.44
144_V182_I1.0750.43
84_Q87_N1.0670.43
36_S78_I1.0620.42
145_S150_H1.0610.42
124_Q143_L1.0610.42
150_H188_L1.0600.42
70_G78_I1.0600.42
45_I99_G1.0560.42
253_P270_D1.0530.42
130_W138_P1.0500.42
90_M160_I1.0440.41
198_R230_P1.0400.41
248_P251_T1.0300.41
246_P253_P1.0250.40
41_S51_L1.0160.40
239_L245_P1.0100.39
16_A231_S1.0090.39
139_Q208_A1.0080.39
81_L163_L0.9970.39
19_A90_M0.9940.38
153_S185_Q0.9930.38
185_Q188_L0.9820.38
16_A25_K0.9740.37
24_Q56_E0.9710.37
195_D267_A0.9560.36
33_S79_Q0.9550.36
63_T136_L0.9460.36
238_E245_P0.9440.36
82_S86_S0.9390.35
84_Q88_L0.9320.35
53_I58_Y0.9290.35
15_S18_L0.9290.35
24_Q106_F0.9270.35
95_I119_V0.9230.34
200_V208_A0.9230.34
24_Q100_M0.9210.34
30_V46_V0.9130.34
42_N258_P0.9050.33
78_I83_S0.9050.33
66_R101_P0.8960.33
149_K179_N0.8950.33
200_V229_P0.8910.33
190_A196_W0.8900.33
182_I186_L0.8890.33
163_L236_G0.8880.33
71_H220_S0.8840.32
162_S165_K0.8810.32
249_G255_K0.8780.32
91_G126_P0.8720.32
21_T260_P0.8720.32
39_S224_E0.8700.32
59_Q109_G0.8700.32
75_V210_M0.8590.31
252_K255_K0.8590.31
77_F80_R0.8570.31
143_L210_M0.8530.31
39_S174_T0.8520.31
222_E264_S0.8510.31
54_Q129_R0.8490.30
31_P158_P0.8480.30
85_V89_G0.8480.30
33_S78_I0.8440.30
191_G218_A0.8440.30
23_W42_N0.8430.30
80_R83_S0.8360.30
61_M92_T0.8350.30
59_Q130_W0.8340.30
35_Q222_E0.8340.30
71_H75_V0.8330.30
111_A119_V0.8290.29
242_W245_P0.8290.29
77_F81_L0.8280.29
79_Q263_P0.8240.29
205_Q242_W0.8150.29
165_K196_W0.8110.29
247_K256_K0.8020.28
53_I63_T0.8020.28
20_A184_Q0.8000.28
174_T244_E0.7980.28
58_Y92_T0.7960.28
266_Q270_D0.7920.28
99_G166_L0.7900.27
165_K189_D0.7840.27
76_M172_D0.7830.27
70_G115_T0.7820.27
119_V123_L0.7800.27
64_D135_L0.7770.27
263_P267_A0.7740.27
184_Q240_Q0.7730.27
191_G231_S0.7730.27
63_T68_Y0.7710.27
34_A86_S0.7660.26
34_A72_P0.7560.26
41_S227_P0.7530.26
18_L266_Q0.7460.25
32_G99_G0.7440.25
48_A255_K0.7400.25
66_R69_F0.7390.25
152_V185_Q0.7360.25
51_L74_L0.7350.25
102_A268_L0.7340.25
163_L169_Q0.7320.25
109_G130_W0.7310.25
18_L258_P0.7290.25
247_K251_T0.7280.25
93_V253_P0.7270.25
100_M105_R0.7250.24
94_L182_I0.7250.24
123_L208_A0.7250.24
232_G236_G0.7220.24
98_M142_D0.7200.24
175_R187_C0.7190.24
48_A117_L0.7190.24
183_K187_C0.7130.24
82_S267_A0.7120.24
203_W212_V0.7120.24
164_I169_Q0.7100.24
165_K169_Q0.7100.24
60_V63_T0.7050.24
81_L176_I0.7020.23
239_L243_F0.7020.23
23_W57_H0.7020.23
119_V125_L0.6970.23
34_A247_K0.6960.23
146_R149_K0.6960.23
16_A48_A0.6960.23
17_S21_T0.6950.23
197_L212_V0.6950.23
249_G256_K0.6950.23
175_R183_K0.6940.23
252_K256_K0.6910.23
196_W200_V0.6910.23
167_A170_D0.6900.23
186_L200_V0.6860.23
196_W212_V0.6800.22
63_T100_M0.6790.22
49_D254_E0.6790.22
40_F143_L0.6760.22
150_H181_A0.6750.22
23_W61_M0.6740.22
222_E225_D0.6730.22
198_R229_P0.6720.22
42_N68_Y0.6720.22
14_S17_S0.6700.22
50_T219_D0.6670.22
29_P52_P0.6630.22
182_I242_W0.6630.22
131_T134_Q0.6620.22
174_T238_E0.6610.22
147_D270_D0.6600.22
39_S75_V0.6580.22
229_P238_E0.6550.21
61_M64_D0.6530.21
29_P194_R0.6520.21
73_D95_I0.6510.21
98_M115_T0.6490.21
32_G51_L0.6480.21
181_A190_A0.6480.21
174_T198_R0.6480.21
123_L178_V0.6470.21
23_W90_M0.6450.21
45_I208_A0.6440.21
100_M142_D0.6440.21
17_S266_Q0.6370.21
117_L183_K0.6370.21
83_S178_V0.6350.21
36_S221_L0.6330.21
42_N261_L0.6320.20
248_P256_K0.6310.20
34_A48_A0.6280.20
55_S135_L0.6270.20
151_V181_A0.6250.20
78_I165_K0.6240.20
126_P134_Q0.6230.20
145_S152_V0.6210.20
149_K184_Q0.6140.20
79_Q171_K0.6120.20
165_K170_D0.6070.19
171_K195_D0.6070.19
135_L138_P0.6060.19
132_S154_T0.6060.19
174_T208_A0.6040.19
45_I129_R0.6030.19
34_A99_G0.6030.19
159_E228_L0.6030.19
65_Q136_L0.6020.19
81_L85_V0.6000.19
144_V179_N0.6000.19
173_V240_Q0.5990.19
19_A261_L0.5990.19
44_C168_A0.5980.19
135_L268_L0.5960.19
66_R143_L0.5910.19
141_L155_L0.5900.19
42_N259_P0.5890.19
21_T42_N0.5880.19
41_S198_R0.5880.19
33_S83_S0.5880.19
40_F174_T0.5870.19
195_D270_D0.5870.19
22_P259_P0.5860.19
37_I115_T0.5850.19
164_I228_L0.5840.19
61_M140_A0.5840.19
61_M82_S0.5810.19
119_V160_I0.5800.18
230_P238_E0.5800.18
251_T255_K0.5790.18
149_K243_F0.5780.18
36_S115_T0.5770.18
165_K190_A0.5730.18
19_A92_T0.5720.18
132_S150_H0.5700.18
35_Q102_A0.5700.18
106_F138_P0.5680.18
15_S132_S0.5680.18
88_L166_L0.5670.18
77_F173_V0.5670.18
31_P224_E0.5660.18
21_T105_R0.5660.18
26_I249_G0.5640.18
119_V137_R0.5630.18
130_W135_L0.5630.18
260_P266_Q0.5620.18
161_F186_L0.5610.18
90_M100_M0.5600.18
36_S101_P0.5570.18
23_W259_P0.5560.18
128_T215_R0.5560.18
218_A253_P0.5540.18
257_T260_P0.5540.18
144_V155_L0.5520.17
263_P266_Q0.5520.17
18_L56_E0.5510.17
51_L214_L0.5500.17
16_A22_P0.5500.17
247_K252_K0.5500.17
94_L144_V0.5500.17
175_R235_C0.5490.17
129_R163_L0.5470.17
188_L266_Q0.5420.17
62_R122_F0.5410.17
101_P122_F0.5410.17
61_M261_L0.5400.17
98_M198_R0.5390.17
34_A93_V0.5370.17
77_F169_Q0.5370.17
74_L95_I0.5370.17
28_Q77_F0.5360.17
203_W238_E0.5360.17
129_R267_A0.5350.17
88_L195_D0.5340.17
187_C235_C0.5340.17
247_K250_T0.5330.17
17_S34_A0.5310.17
23_W258_P0.5310.17
107_N150_H0.5280.17
123_L200_V0.5280.17
182_I252_K0.5240.17
36_S99_G0.5230.16
48_A208_A0.5220.16
57_H129_R0.5220.16
34_A162_S0.5210.16
73_D193_D0.5200.16
149_K181_A0.5190.16
16_A270_D0.5190.16
86_S171_K0.5180.16
41_S261_L0.5180.16
81_L93_V0.5180.16
166_L180_P0.5160.16
61_M90_M0.5160.16
35_Q88_L0.5160.16
87_N162_S0.5150.16
16_A266_Q0.5150.16
252_K257_T0.5140.16
168_A201_R0.5130.16
26_I266_Q0.5120.16
99_G205_Q0.5100.16
60_V68_Y0.5080.16
156_W160_I0.5070.16
55_S122_F0.5070.16
63_T105_R0.5070.16
99_G160_I0.5070.16
55_S160_I0.5060.16
217_P229_P0.5050.16
27_T95_I0.5050.16
248_P255_K0.5040.16
30_V102_A0.5030.16
73_D255_K0.5030.16
153_S264_S0.5020.16
52_P117_L0.5010.16
51_L170_D0.5000.16
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1tzpA 2 0.8796 100 0.173 Contact Map
3fefA 3 0.5365 24 0.975 Contact Map
4nt9B 3 0.5547 12.4 0.978 Contact Map
4mphA 2 0.5511 8.7 0.979 Contact Map
2mxzA 1 0.3759 7 0.98 Contact Map
2exnA 1 0.4051 6.9 0.98 Contact Map
2vo9A 1 0.4124 6.7 0.98 Contact Map
2p5iA 1 0.6825 5.2 0.981 Contact Map
1cf4B 1 0.1533 4.7 0.982 Contact Map
2p0lA 1 0.6569 4.6 0.982 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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