GREMLIN Database
MSCS - Small-conductance mechanosensitive channel
UniProt: P0C0S1 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11160
Length: 286 (249)
Sequences: 11430 (8334)
Seq/√Len: 528.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
258_K262_D3.6471.00
199_D259_R3.4411.00
137_D142_A3.3461.00
41_G81_T3.1671.00
206_T210_Q3.1181.00
146_L156_R3.1171.00
202_K255_E3.0011.00
227_E234_N2.9211.00
260_E263_A2.6891.00
260_E264_A2.6771.00
200_Q264_A2.5801.00
212_E252_D2.5421.00
135_Y142_A2.4801.00
169_K173_G2.4601.00
226_N236_V2.4411.00
229_G234_N2.3391.00
112_Q116_S2.2771.00
191_G232_S2.1981.00
203_Q207_N2.1791.00
197_D200_Q2.1541.00
207_N260_E2.1531.00
189_I234_N2.1511.00
200_Q203_Q2.1481.00
207_N211_S2.1371.00
189_I236_V2.1321.00
205_L257_I2.0031.00
200_Q204_I1.9731.00
147_S154_T1.9641.00
250_Y254_L1.9471.00
135_Y144_T1.9321.00
256_R259_R1.9211.00
204_I260_E1.8491.00
137_D178_F1.8131.00
193_A267_S1.8061.00
191_G234_N1.7651.00
252_D256_R1.7431.00
192_V201_V1.7341.00
208_I256_R1.7311.00
208_I260_E1.7041.00
225_L233_I1.7031.00
204_I264_A1.6961.00
146_L162_I1.6951.00
211_S256_R1.6651.00
202_K206_T1.6441.00
239_V253_V1.6221.00
196_S267_S1.6181.00
259_R263_A1.6101.00
157_T163_I1.5841.00
199_D255_E1.5701.00
137_D176_I1.5671.00
137_D140_G1.5551.00
127_F151_F1.5511.00
139_G174_N1.5321.00
216_L219_R1.5221.00
209_I237_V1.4991.00
212_E256_R1.4811.00
135_Y158_A1.4771.00
261_F266_I1.4621.00
141_V174_N1.4561.00
113_G116_S1.4491.00
187_E236_V1.4451.00
139_G173_G1.3991.00
214_R245_D1.3801.00
130_F153_T1.3731.00
241_S249_V1.3731.00
53_N70_S1.3711.00
154_T162_I1.3651.00
49_S74_R1.3621.00
34_A89_V1.3561.00
161_K176_I1.3461.00
163_I176_I1.3371.00
201_V235_F1.3291.00
43_I47_M1.3271.00
245_D248_N1.3261.00
136_V145_V1.3191.00
190_I257_I1.3131.00
153_T167_N1.3131.00
211_S260_E1.3111.00
255_E259_R1.3021.00
41_G45_A1.3011.00
199_D203_Q1.2971.00
105_L108_G1.2831.00
248_N252_D1.2681.00
134_E180_R1.2541.00
256_R260_E1.2441.00
86_L91_V1.2161.00
30_N33_A1.2091.00
205_L237_V1.2081.00
246_L250_Y1.2001.00
38_I42_L1.1931.00
192_V196_S1.1891.00
207_N210_Q1.1851.00
77_I81_T1.1771.00
204_I207_N1.1761.00
230_A273_M1.1761.00
186_N239_V1.1741.00
74_R78_I1.1681.00
143_G155_M1.1601.00
131_R180_R1.1581.00
214_R252_D1.1531.00
85_A89_V1.1311.00
193_A196_S1.1271.00
169_K172_A1.1191.00
189_I227_E1.1131.00
162_I180_R1.1111.00
169_K174_N1.1111.00
230_A234_N1.1071.00
49_S70_S1.0971.00
195_D258_K1.0901.00
242_N245_D1.0731.00
34_A85_A1.0721.00
99_V102_A1.0721.00
195_D270_Y1.0701.00
138_L170_I1.0601.00
157_T161_K1.0561.00
203_Q206_T1.0551.00
195_D267_S1.0541.00
199_D202_K1.0491.00
191_G229_G1.0441.00
196_S201_V1.0351.00
206_T223_V1.0311.00
194_Y228_L1.0261.00
226_N234_N1.0221.00
210_Q217_K1.0181.00
95_S98_A1.0021.00
185_R240_W0.9771.00
184_R246_L0.9761.00
189_I226_N0.9711.00
186_N250_Y0.9681.00
111_L119_A0.9591.00
223_V237_V0.9501.00
253_V257_I0.9371.00
51_A55_L0.9351.00
27_Y31_I0.9311.00
152_S164_V0.9281.00
29_V33_A0.9211.00
251_W255_E0.9131.00
187_E238_R0.9101.00
209_I239_V0.9091.00
103_A107_V0.9081.00
192_V235_F0.9041.00
42_L46_R0.9031.00
206_T221_M0.9021.00
198_I235_F0.9021.00
149_Q154_T0.9001.00
42_L78_I0.8961.00
45_A49_S0.8961.00
191_G231_S0.8951.00
110_A115_L0.8931.00
160_G240_W0.8931.00
37_I81_T0.8861.00
196_S200_Q0.8841.00
97_I101_G0.8811.00
156_R162_I0.8801.00
261_F268_F0.8741.00
144_T156_R0.8731.00
214_R249_V0.8701.00
214_R248_N0.8681.00
212_E215_I0.8681.00
67_D71_A0.8641.00
45_A74_R0.8611.00
123_L171_I0.8611.00
198_I202_K0.8591.00
192_V266_I0.8561.00
37_I88_R0.8561.00
227_E254_L0.8541.00
267_S274_D0.8541.00
212_E253_V0.8501.00
204_I266_I0.8501.00
228_L233_I0.8481.00
231_S270_Y0.8471.00
81_T85_A0.8451.00
116_S168_G0.8431.00
53_N57_I0.8431.00
191_G230_A0.8411.00
70_S74_R0.8401.00
136_V155_M0.8371.00
270_Y274_D0.8371.00
224_R251_W0.8351.00
198_I233_I0.8261.00
33_A88_R0.8231.00
247_Q251_W0.8211.00
208_I253_V0.8211.00
78_I82_L0.8201.00
145_V155_M0.8201.00
252_D255_E0.8121.00
139_G169_K0.8081.00
162_I181_E0.8081.00
225_L254_L0.8071.00
114_S120_A0.8031.00
128_R131_R0.8021.00
202_K251_W0.8001.00
87_G92_Q0.7961.00
193_A269_P0.7951.00
224_R236_V0.7881.00
141_V163_I0.7851.00
102_A111_L0.7851.00
192_V261_F0.7821.00
39_I43_I0.7811.00
120_A171_I0.7781.00
198_I258_K0.7741.00
41_G77_I0.7621.00
46_R50_N0.7611.00
156_R160_G0.7561.00
216_L242_N0.7541.00
205_L261_F0.7481.00
188_F254_L0.7481.00
127_F152_S0.7451.00
28_A31_I0.7401.00
226_N250_Y0.7391.00
194_Y268_F0.7311.00
99_V103_A0.7311.00
186_N241_S0.7301.00
191_G194_Y0.7281.00
45_A81_T0.7281.00
190_I205_L0.7241.00
107_V115_L0.7231.00
230_A271_P0.7211.00
228_L254_L0.7211.00
32_V36_A0.7171.00
229_G232_S0.7121.00
205_L209_I0.7081.00
48_I73_V0.7071.00
117_N168_G0.7021.00
156_R181_E0.6951.00
194_Y233_I0.6941.00
45_A77_I0.6931.00
209_I253_V0.6901.00
130_F170_I0.6891.00
188_F239_V0.6891.00
203_Q264_A0.6881.00
46_R49_S0.6861.00
237_V257_I0.6851.00
194_Y270_Y0.6841.00
99_V104_G0.6831.00
126_M129_P0.6801.00
231_S273_M0.6791.00
103_A106_A0.6771.00
209_I215_I0.6761.00
53_N66_A0.6731.00
204_I208_I0.6721.00
138_L165_I0.6701.00
43_I46_R0.6621.00
267_S272_Q0.6611.00
38_I41_G0.6601.00
222_T238_R0.6571.00
233_I258_K0.6531.00
52_V55_L0.6521.00
215_I249_V0.6501.00
149_Q152_S0.6491.00
81_T84_A0.6481.00
193_A268_F0.6481.00
26_S30_N0.6481.00
100_L104_G0.6471.00
208_I211_S0.6461.00
208_I212_E0.6451.00
112_Q168_G0.6441.00
197_D259_R0.6441.00
185_R238_R0.6431.00
247_Q250_Y0.6431.00
144_T244_G0.6421.00
205_L223_V0.6421.00
116_S172_A0.6421.00
137_D141_V0.6411.00
206_T220_E0.6391.00
231_S271_P0.6371.00
92_Q95_S0.6371.00
27_Y30_N0.6351.00
154_T164_V0.6341.00
67_D147_S0.6341.00
37_I41_G0.6321.00
213_D217_K0.6311.00
138_L141_V0.6301.00
114_S171_I0.6291.00
206_T209_I0.6291.00
47_M50_N0.6281.00
79_A83_I0.6271.00
142_A178_F0.6231.00
141_V176_I0.6221.00
32_V35_L0.6201.00
36_A39_I0.6171.00
64_T125_V0.6171.00
45_A78_I0.6161.00
210_Q220_E0.6161.00
228_L258_K0.6151.00
201_V261_F0.6131.00
130_F136_V0.6121.00
107_V119_A0.6121.00
98_A101_G0.6111.00
48_I51_A0.6111.00
127_F154_T0.6111.00
191_G270_Y0.6101.00
209_I223_V0.6101.00
101_G104_G0.6091.00
120_A168_G0.6071.00
102_A106_A0.6041.00
84_A88_R0.6031.00
224_R247_Q0.6031.00
207_N264_A0.5991.00
214_R217_K0.5981.00
57_I66_A0.5971.00
203_Q210_Q0.5971.00
183_V242_N0.5941.00
231_S272_Q0.5941.00
97_I106_A0.5920.99
48_I77_I0.5900.99
94_A98_A0.5890.99
113_G168_G0.5870.99
47_M51_A0.5850.99
54_R58_S0.5830.99
113_G172_A0.5820.99
190_I237_V0.5810.99
200_Q207_N0.5810.99
192_V198_I0.5800.99
40_V44_I0.5800.99
201_V266_I0.5790.99
227_E250_Y0.5760.99
209_I221_M0.5760.99
63_A66_A0.5760.99
270_Y273_M0.5750.99
159_D185_R0.5720.99
71_A74_R0.5710.99
66_A70_S0.5700.99
34_A38_I0.5690.99
136_V170_I0.5690.99
63_A67_D0.5680.99
102_A105_L0.5670.99
196_S233_I0.5660.99
210_Q221_M0.5640.99
28_A32_V0.5630.99
186_N246_L0.5610.99
36_A40_V0.5590.99
193_A270_Y0.5540.99
195_D262_D0.5520.99
194_Y230_A0.5510.99
254_L257_I0.5510.99
71_A75_Y0.5510.99
130_F175_I0.5480.99
197_D201_V0.5460.99
29_V32_V0.5460.99
183_V219_R0.5450.99
49_S53_N0.5420.99
212_E217_K0.5420.99
204_I261_F0.5410.99
228_L257_I0.5400.99
35_L46_R0.5400.99
105_L113_G0.5370.99
165_I174_N0.5350.99
261_F264_A0.5320.99
109_L112_Q0.5320.99
115_L119_A0.5260.99
161_K178_F0.5260.99
144_T158_A0.5260.99
124_L130_F0.5240.99
84_A87_G0.5230.99
268_F272_Q0.5230.99
64_T68_F0.5220.99
198_I259_R0.5200.99
100_L103_A0.5190.99
51_A54_R0.5190.99
208_I264_A0.5190.99
39_I42_L0.5170.99
208_I261_F0.5140.99
193_A274_D0.5140.99
55_L58_S0.5120.99
80_F84_A0.5090.98
197_D262_D0.5080.98
202_K235_F0.5060.98
146_L181_E0.5060.98
259_R262_D0.5040.98
168_G172_A0.5040.98
45_A70_S0.5020.98
138_L173_G0.5010.98
222_T248_N0.5000.98
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
5ajiA 3 0.9196 100 0.103 Contact Map
4hw9A 3 0.8811 100 0.157 Contact Map
3udcA 3 0.8881 100 0.2 Contact Map
1nz9A 1 0.1678 23.9 0.948 Contact Map
2mi6A 1 0.1818 17 0.952 Contact Map
2e6zA 1 0.1783 16.8 0.952 Contact Map
3mlqE 1 0.1364 13.9 0.954 Contact Map
3tzuA 2 0.4126 11.8 0.955 Contact Map
2lt1A 1 0.2203 11.4 0.955 Contact Map
1ifkA 9 0.1783 9.3 0.957 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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