PSTS - ECOLI - GREMLIN Contacts

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PSTS - Phosphate-binding protein PstS

UniProt:	P0AG82	Sequences: MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIGGVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRRADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGYVEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWPITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWKTNIKDSSGKPLY	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG10734
Length:	346 (282)
Sequences:	5261 (3795)
Seq/√Len:	226.0

ECOLI - PSTS
BACSU - PSTS
HALSA - Q9HML5 Q9HS10
SULSO - Q97ZT8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
127_D	132_K	4.030	1.00
121_D	124_T	3.885	1.00
135_K	152_S	3.648	1.00
137_D	152_S	3.444	1.00
153_Q	214_G	3.410	1.00
128_I	155_I	3.274	1.00
119_V	241_P	3.212	1.00
233_K	243_S	2.929	1.00
120_L	232_T	2.861	1.00
121_D	144_L	2.832	1.00
111_P	213_P	2.580	1.00
135_K	154_N	2.519	1.00
123_K	127_D	2.465	1.00
32_A	75_V	2.397	1.00
106_L	125_L	2.382	1.00
124_T	145_N	2.326	1.00
107_A	209_V	2.300	1.00
35_T	60_G	2.201	1.00
34_A	37_P	2.186	1.00
145_N	149_K	2.158	1.00
124_T	127_D	2.143	1.00
32_A	69_Q	2.134	1.00
48_Y	300_K	2.122	1.00
86_D	90_A	2.121	1.00
61_I	65_G	2.112	1.00
95_F	287_K	2.044	1.00
245_T	248_N	2.043	1.00
119_V	233_K	2.018	1.00
235_I	241_P	2.013	1.00
323_D	327_E	2.005	1.00
47_T	304_W	2.003	1.00
47_T	50_K	2.000	1.00
156_A	212_L	1.972	1.00
169_V	279_S	1.950	1.00
107_A	219_V	1.903	1.00
41_Y	301_F	1.891	1.00
124_T	144_L	1.883	1.00
327_E	331_A	1.881	1.00
38_A	60_G	1.869	1.00
118_L	230_A	1.863	1.00
63_S	80_S	1.830	1.00
51_E	308_T	1.808	1.00
303_D	330_R	1.807	1.00
82_A	88_K	1.770	1.00
108_V	232_T	1.763	1.00
121_D	235_I	1.755	1.00
106_L	170_F	1.687	1.00
48_Y	54_N	1.680	1.00
135_K	153_Q	1.660	1.00
129_Y	157_V	1.628	1.00
41_Y	305_A	1.615	1.00
120_L	125_L	1.614	1.00
76_D	294_Q	1.597	1.00
126_G	178_N	1.580	1.00
224_A	231_Y	1.569	1.00
209_V	217_G	1.552	1.00
125_L	234_L	1.533	1.00
103_G	249_F	1.514	1.00
31_G	77_F	1.491	1.00
38_A	42_A	1.461	1.00
134_K	138_D	1.449	1.00
296_T	300_K	1.423	1.00
153_Q	213_P	1.414	1.00
30_T	57_N	1.412	1.00
69_Q	75_V	1.411	1.00
104_V	279_S	1.398	1.00
133_I	138_D	1.386	1.00
327_E	330_R	1.379	1.00
283_I	305_A	1.376	1.00
49_Q	55_K	1.375	1.00
60_G	203_D	1.374	1.00
80_S	284_L	1.355	1.00
70_I	78_G	1.354	1.00
291_K	294_Q	1.348	1.00
329_V	332_A	1.326	1.00
207_A	211_R	1.321	1.00
27_A	297_E	1.314	1.00
15_T	18_M	1.294	1.00
127_D	133_I	1.292	1.00
206_A	219_V	1.277	1.00
202_N	223_Y	1.258	1.00
37_P	41_Y	1.258	1.00
107_A	229_L	1.255	1.00
326_V	330_R	1.250	1.00
328_Q	331_A	1.237	1.00
157_V	216_I	1.233	1.00
118_L	233_K	1.229	1.00
98_P	329_V	1.221	1.00
314_N	320_S	1.215	1.00
246_E	250_A	1.213	1.00
285_I	302_F	1.211	1.00
307_K	330_R	1.205	1.00
100_V	281_T	1.205	1.00
120_L	128_I	1.193	1.00
85_S	88_K	1.189	1.00
41_Y	283_I	1.183	1.00
48_Y	304_W	1.177	1.00
44_W	305_A	1.176	1.00
15_T	19_S	1.173	1.00
97_F	306_Y	1.171	1.00
28_S	57_N	1.166	1.00
158_V	208_F	1.164	1.00
28_S	55_K	1.163	1.00
306_Y	310_A	1.162	1.00
25_A	54_N	1.144	1.00
41_Y	99_T	1.143	1.00
289_Q	295_G	1.140	1.00
300_K	303_D	1.134	1.00
13_A	16_L	1.132	1.00
67_V	84_L	1.132	1.00
105_V	224_A	1.127	1.00
311_K	315_D	1.123	1.00
43_K	315_D	1.123	1.00
135_K	150_L	1.121	1.00
236_S	239_G	1.112	1.00
18_M	21_F	1.103	1.00
160_R	164_S	1.101	1.00
246_E	314_N	1.100	1.00
234_L	277_I	1.096	1.00
159_R	198_G	1.091	1.00
97_F	285_I	1.086	1.00
32_A	61_I	1.082	1.00
173_Y	177_V	1.077	1.00
73_N	286_H	1.076	1.00
47_T	312_Q	1.052	1.00
84_L	94_L	1.050	1.00
235_I	239_G	1.048	1.00
303_D	307_K	1.046	1.00
79_A	283_I	1.045	1.00
158_V	212_L	1.042	1.00
34_A	81_D	1.042	1.00
306_Y	329_V	1.036	1.00
122_G	174_L	1.032	1.00
104_V	169_V	1.031	1.00
12_V	15_T	1.021	1.00
129_Y	170_F	1.020	1.00
59_Q	69_Q	1.017	1.00
328_Q	332_A	1.013	1.00
67_V	80_S	1.011	1.00
34_A	41_Y	1.007	1.00
119_V	235_I	0.987	1.00
36_F	102_G	0.987	1.00
87_E	90_A	0.984	1.00
242_V	274_A	0.983	1.00
132_K	180_E	0.983	1.00
21_F	25_A	0.981	1.00
310_A	326_V	0.973	1.00
71_I	91_Q	0.966	1.00
41_Y	79_A	0.961	1.00
225_K	228_N	0.960	1.00
11_V	14_A	0.952	1.00
75_V	78_G	0.947	1.00
36_F	281_T	0.945	1.00
299_L	303_D	0.943	1.00
101_I	280_T	0.938	1.00
43_K	46_D	0.934	1.00
45_A	56_V	0.927	1.00
301_F	305_A	0.927	1.00
99_T	281_T	0.927	1.00
324_S	327_E	0.927	1.00
222_A	318_Y	0.925	1.00
8_V	12_V	0.922	1.00
199_G	205_I	0.920	1.00
106_L	275_W	0.920	1.00
99_T	283_I	0.920	1.00
19_S	22_S	0.920	1.00
158_V	197_L	0.919	1.00
323_D	326_V	0.917	1.00
123_K	126_G	0.916	1.00
134_K	154_N	0.914	0.99
40_V	100_V	0.913	0.99
221_Y	244_P	0.903	0.99
86_D	89_L	0.899	0.99
18_M	23_V	0.895	0.99
45_A	301_F	0.892	0.99
83_P	96_Q	0.886	0.99
84_L	284_L	0.885	0.99
68_K	72_A	0.883	0.99
325_V	328_Q	0.881	0.99
45_A	49_Q	0.880	0.99
221_Y	225_K	0.873	0.99
156_A	197_L	0.870	0.99
202_N	219_V	0.865	0.99
45_A	58_Y	0.864	0.99
234_L	275_W	0.861	0.99
158_V	215_A	0.859	0.99
167_S	218_Y	0.858	0.99
18_M	22_S	0.850	0.99
69_Q	74_T	0.845	0.99
77_F	283_I	0.844	0.99
98_P	280_T	0.844	0.99
82_A	168_F	0.843	0.99
37_P	99_T	0.843	0.99
29_L	297_E	0.842	0.99
122_G	126_G	0.840	0.99
242_V	248_N	0.836	0.99
102_G	279_S	0.835	0.99
104_V	166_T	0.833	0.99
145_N	148_L	0.832	0.99
100_V	313_A	0.832	0.99
208_F	212_L	0.830	0.99
20_A	24_F	0.830	0.99
64_S	163_G	0.827	0.99
81_D	281_T	0.826	0.99
250_A	319_A	0.824	0.99
102_G	220_E	0.820	0.99
159_R	196_G	0.820	0.99
144_L	148_L	0.819	0.99
48_Y	301_F	0.818	0.99
108_V	230_A	0.816	0.99
312_Q	315_D	0.816	0.99
130_L	180_E	0.813	0.99
304_W	312_Q	0.811	0.99
248_N	274_A	0.807	0.99
21_F	24_F	0.805	0.99
306_Y	330_R	0.804	0.99
135_K	149_K	0.802	0.99
84_L	96_Q	0.799	0.99
67_V	78_G	0.798	0.99
197_L	208_F	0.798	0.99
96_Q	284_L	0.797	0.99
88_K	91_Q	0.796	0.99
127_D	130_L	0.792	0.99
98_P	325_V	0.792	0.99
61_I	69_Q	0.788	0.98
201_G	204_G	0.788	0.98
205_I	217_G	0.787	0.98
137_D	145_N	0.786	0.98
180_E	184_N	0.784	0.98
36_F	279_S	0.784	0.98
70_I	94_L	0.784	0.98
164_S	202_N	0.783	0.98
14_A	17_S	0.783	0.98
44_W	309_G	0.776	0.98
32_A	59_Q	0.776	0.98
283_I	302_F	0.773	0.98
36_F	81_D	0.773	0.98
62_G	66_G	0.767	0.98
9_A	14_A	0.766	0.98
89_L	94_L	0.763	0.98
128_I	148_L	0.763	0.98
126_G	174_L	0.761	0.98
129_Y	216_I	0.761	0.98
249_F	278_T	0.757	0.98
120_L	150_L	0.754	0.98
122_G	173_Y	0.752	0.98
204_G	208_F	0.748	0.98
43_K	316_L	0.747	0.98
114_K	230_A	0.745	0.98
11_V	15_T	0.742	0.98
77_F	285_I	0.741	0.98
67_V	88_K	0.741	0.98
37_P	283_I	0.740	0.98
322_P	325_V	0.738	0.98
160_R	218_Y	0.738	0.98
43_K	47_T	0.736	0.98
144_L	241_P	0.729	0.98
135_K	138_D	0.727	0.97
137_D	150_L	0.727	0.97
248_N	273_D	0.721	0.97
10_T	13_A	0.717	0.97
248_N	272_E	0.716	0.97
243_S	248_N	0.715	0.97
311_K	314_N	0.711	0.97
105_V	221_Y	0.710	0.97
39_P	223_Y	0.709	0.97
81_D	279_S	0.702	0.97
158_V	199_G	0.702	0.97
102_G	281_T	0.701	0.97
34_A	165_G	0.701	0.97
8_V	11_V	0.699	0.97
325_V	329_V	0.698	0.97
113_L	151_P	0.695	0.97
126_G	130_L	0.692	0.97
96_Q	282_F	0.691	0.97
137_D	149_K	0.688	0.96
161_A	200_K	0.685	0.96
13_A	17_S	0.681	0.96
244_P	248_N	0.678	0.96
231_Y	244_P	0.677	0.96
122_G	238_D	0.677	0.96
210_Q	228_N	0.676	0.96
97_F	329_V	0.673	0.96
22_S	25_A	0.671	0.96
37_P	281_T	0.670	0.96
286_H	289_Q	0.669	0.96
172_S	176_K	0.669	0.96
16_L	19_S	0.666	0.96
219_V	223_Y	0.665	0.96
40_V	313_A	0.662	0.96
105_V	231_Y	0.660	0.96
120_L	234_L	0.658	0.95
246_E	320_S	0.656	0.95
247_E	250_A	0.655	0.95
17_S	22_S	0.654	0.95
110_I	214_G	0.654	0.95
17_S	20_A	0.652	0.95
62_G	81_D	0.652	0.95
47_T	51_E	0.652	0.95
81_D	102_G	0.652	0.95
106_L	129_Y	0.649	0.95
171_T	175_A	0.648	0.95
168_F	172_S	0.647	0.95
310_A	313_A	0.643	0.95
108_V	118_L	0.642	0.95
199_G	208_F	0.639	0.95
121_D	241_P	0.639	0.95
164_S	200_K	0.638	0.95
206_A	225_K	0.637	0.95
12_V	16_L	0.635	0.95
66_G	165_G	0.635	0.95
166_T	202_N	0.631	0.94
38_A	61_I	0.629	0.94
138_D	150_L	0.628	0.94
129_Y	159_R	0.627	0.94
220_E	223_Y	0.626	0.94
34_A	99_T	0.624	0.94
181_W	185_V	0.623	0.94
65_G	69_Q	0.621	0.94
84_L	89_L	0.620	0.94
82_A	85_S	0.618	0.94
158_V	205_I	0.618	0.94
105_V	244_P	0.614	0.93
120_L	124_T	0.613	0.93
109_N	213_P	0.613	0.93
233_K	241_P	0.613	0.93
105_V	249_F	0.611	0.93
297_E	300_K	0.611	0.93
221_Y	246_E	0.608	0.93
325_V	332_A	0.608	0.93
34_A	283_I	0.607	0.93
27_A	54_N	0.605	0.93
308_T	311_K	0.605	0.93
54_N	297_E	0.604	0.93
235_I	238_D	0.602	0.93
31_G	79_A	0.602	0.93
25_A	48_Y	0.601	0.93
79_A	305_A	0.599	0.93
59_Q	65_G	0.598	0.93
35_T	201_G	0.598	0.93
302_F	306_Y	0.597	0.93
125_L	129_Y	0.596	0.92
169_V	173_Y	0.596	0.92
122_G	276_P	0.594	0.92
30_T	59_Q	0.593	0.92
124_T	133_I	0.591	0.92
155_I	214_G	0.590	0.92
164_S	205_I	0.589	0.92
20_A	23_V	0.587	0.92
134_K	152_S	0.585	0.92
233_K	244_P	0.581	0.91
180_E	183_N	0.580	0.91
54_N	300_K	0.579	0.91
199_G	204_G	0.578	0.91
111_P	214_G	0.577	0.91
99_T	305_A	0.576	0.91
212_L	215_A	0.574	0.91
225_K	317_D	0.574	0.91
157_V	181_W	0.573	0.91
62_G	166_T	0.572	0.91
83_P	282_F	0.570	0.91
170_F	277_I	0.569	0.91
107_A	217_G	0.567	0.90
156_A	214_G	0.566	0.90
63_S	66_G	0.562	0.90
161_A	201_G	0.561	0.90
41_Y	58_Y	0.559	0.90
122_G	144_L	0.559	0.90
17_S	21_F	0.558	0.90
57_N	69_Q	0.558	0.90
7_T	11_V	0.558	0.90
285_I	298_V	0.555	0.90
302_F	305_A	0.554	0.89
113_L	230_A	0.553	0.89
324_S	328_Q	0.552	0.89
13_A	18_M	0.551	0.89
11_V	17_S	0.551	0.89
160_R	202_N	0.549	0.89
124_T	149_K	0.547	0.89
123_K	180_E	0.545	0.89
128_I	144_L	0.545	0.89
175_A	178_N	0.541	0.88
14_A	24_F	0.540	0.88
207_A	210_Q	0.539	0.88
10_T	14_A	0.539	0.88
305_A	309_G	0.538	0.88
89_L	96_Q	0.537	0.88
48_Y	297_E	0.534	0.88
18_M	24_F	0.531	0.87
307_K	326_V	0.530	0.87
32_A	65_G	0.530	0.87
303_D	306_Y	0.529	0.87
97_F	302_F	0.529	0.87
36_F	166_T	0.525	0.87
68_K	91_Q	0.524	0.87
307_K	310_A	0.522	0.87
173_Y	176_K	0.521	0.86
295_G	298_V	0.518	0.86
118_L	232_T	0.518	0.86
121_D	125_L	0.517	0.86
35_T	62_G	0.516	0.86
62_G	65_G	0.515	0.86
76_D	298_V	0.513	0.86
120_L	216_I	0.512	0.85
167_S	171_T	0.512	0.85
71_I	94_L	0.511	0.85
135_K	214_G	0.511	0.85
68_K	85_S	0.509	0.85
130_L	175_A	0.509	0.85
118_L	150_L	0.508	0.85
166_T	279_S	0.508	0.85
304_W	308_T	0.507	0.85
81_D	166_T	0.506	0.85
209_V	215_A	0.506	0.85
84_L	88_K	0.505	0.85
181_W	184_N	0.505	0.85
11_V	16_L	0.504	0.85
130_L	183_N	0.503	0.84
69_Q	72_A	0.502	0.84
87_E	91_Q	0.502	0.84
44_W	47_T	0.500	0.84
102_G	318_Y	0.500	0.84
221_Y	249_F	0.500	0.84

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4f1vA	1	0.8844	100	0.355	Contact Map
1ixhA	1	0.922	100	0.459	Contact Map
1pc3A	1	0.9249	100	0.499	Contact Map
4n13A	1	0.7023	100	0.512	Contact Map
4lvqA	1	0.8728	100	0.595	Contact Map
4jwoA	1	0.7803	100	0.638	Contact Map
4ombA	1	0.7803	100	0.64	Contact Map
4ecfA	1	0.7399	99.9	0.653	Contact Map
4exlA	1	0.7514	99.9	0.657	Contact Map
1twyA	5	0.7139	99.9	0.658	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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