GREMLIN Database
SUBI - Sulfate-binding protein
UniProt: P0AG78 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10929
Length: 329 (313)
Sequences: 1400 (787)
Seq/√Len: 44.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
250_E255_K5.0161.00
272_E289_K4.5811.00
156_Y316_H4.5051.00
161_G300_F4.4611.00
259_E263_K3.7831.00
48_K54_N3.6941.00
71_V80_V3.5551.00
272_E290_Y3.2731.00
311_K315_E3.2191.00
268_P283_D3.1851.00
249_V255_K3.0971.00
46_H50_Q3.0591.00
132_N166_H2.9211.00
255_K259_E2.9141.00
39_Y264_Y2.8141.00
175_Q325_Q2.6421.00
165_H298_K2.6051.00
248_V252_K2.4351.00
135_I181_L2.4261.00
69_T73_N2.4201.00
42_A273_I2.4081.00
38_Q277_N2.3671.00
72_I91_I2.3641.00
55_V257_V2.3471.00
170_D173_K2.3281.00
164_L174_A2.2161.00
118_F207_V2.1501.00
176_D180_A2.1491.00
171_Q315_E2.1391.00
180_A184_N2.0860.99
270_G274_A2.0570.99
108_N239_E2.0360.99
268_P286_V2.0220.99
216_L231_T1.9960.99
140_S186_E1.9240.99
39_Y267_S1.9170.99
42_A270_G1.9100.99
22_I53_D1.8940.99
107_D282_R1.8860.99
250_E259_E1.8790.99
45_A49_Q1.8720.99
127_Q136_K1.8630.99
38_Q276_K1.8600.99
167_N174_A1.8490.99
76_E252_K1.8340.99
267_S270_G1.8190.99
162_Y177_F1.8180.99
142_I186_E1.8110.98
132_N162_Y1.7930.98
272_E275_A1.7790.98
100_E103_K1.7720.98
104_R246_D1.7490.98
130_D233_S1.7310.98
47_W53_D1.7300.98
24_L258_A1.7270.98
174_A178_V1.6850.98
164_L171_Q1.6750.97
93_E310_T1.6690.97
22_I257_V1.6450.97
179_R183_K1.6370.97
288_K291_E1.6330.97
35_L273_I1.6100.97
141_V181_L1.6040.97
167_N170_D1.5860.96
110_A241_T1.5840.96
268_P285_E1.5810.96
21_D54_N1.5470.96
35_L274_A1.5450.96
167_N173_K1.5440.96
104_R243_S1.5290.96
91_I97_I1.5110.95
98_D104_R1.5080.95
314_K318_A1.5010.95
244_V259_E1.4840.95
92_A97_I1.4740.95
105_L244_V1.4740.95
260_A263_K1.4680.95
39_Y270_G1.4650.94
246_D255_K1.4600.94
164_L167_N1.4600.94
76_E251_K1.4440.94
39_Y274_A1.4270.94
46_H264_Y1.4220.94
140_S184_N1.4150.93
19_A53_D1.4120.93
23_Q58_R1.3990.93
246_D249_V1.3910.93
283_D286_V1.3900.93
300_F305_E1.3840.93
173_K176_D1.3590.92
41_K45_A1.3500.92
275_A286_V1.3470.92
303_D308_G1.3430.91
272_E286_V1.3280.91
76_E248_V1.3190.91
220_N278_Y1.3070.90
246_D250_E1.3060.90
302_I308_G1.3000.90
17_V21_D1.2990.90
39_Y46_H1.2980.90
122_K228_E1.2940.90
143_T155_N1.2740.89
141_V186_E1.2500.88
97_I243_S1.2490.88
97_I109_S1.2340.88
164_L305_E1.2240.87
264_Y267_S1.2200.87
273_I277_N1.2120.87
47_W257_V1.2110.87
118_F209_I1.2090.86
92_A98_D1.1850.85
197_N201_E1.1810.85
315_E322_T1.1790.85
85_A102_I1.1700.85
88_V241_T1.1550.84
92_A100_E1.1380.83
24_L257_V1.1320.83
216_L295_P1.1280.82
98_D109_S1.1230.82
153_R313_Q1.1220.82
70_S75_I1.1170.82
306_F312_A1.0950.80
138_G184_N1.0920.80
175_Q322_T1.0920.80
220_N293_A1.0900.80
222_L278_Y1.0800.79
51_T54_N1.0740.79
100_E104_R1.0720.79
88_V91_I1.0710.79
53_D256_E1.0650.78
198_T204_I1.0640.78
147_K324_D1.0590.78
286_V290_Y1.0550.78
180_A183_K1.0530.78
290_Y293_A1.0510.78
151_G155_N1.0470.77
308_G311_K1.0420.77
83_A211_W1.0380.77
46_H49_Q1.0370.77
291_E296_K1.0330.76
137_P184_N1.0290.76
170_D174_A1.0230.76
105_L243_S1.0160.75
177_F181_L1.0070.74
151_G192_A1.0050.74
249_V254_T0.9920.73
256_E259_E0.9840.73
152_A155_N0.9800.72
46_H269_E0.9770.72
160_W178_V0.9760.72
65_G84_L0.9730.72
268_P284_A0.9730.72
89_D93_E0.9680.71
231_T295_P0.9590.71
112_Y240_P0.9580.71
286_V291_E0.9570.71
192_A211_W0.9550.70
102_I241_T0.9490.70
105_L263_K0.9470.70
42_A45_A0.9460.70
272_E276_K0.9380.69
160_W164_L0.9380.69
72_I90_A0.9370.69
249_V259_E0.9370.69
231_T297_L0.9330.69
84_L154_W0.9300.68
220_N224_K0.9270.68
48_K51_T0.9160.67
65_G154_W0.9140.67
37_E41_K0.9080.67
302_I312_A0.9080.67
21_D53_D0.9030.66
29_Y151_G0.9020.66
65_G83_A0.8940.65
117_V215_A0.8910.65
42_A264_Y0.8900.65
137_P180_A0.8900.65
31_P211_W0.8810.64
48_K52_G0.8810.64
196_T208_L0.8800.64
216_L221_E0.8800.64
98_D243_S0.8790.64
163_A169_N0.8780.64
234_E300_F0.8770.64
42_A269_E0.8760.64
320_G324_D0.8640.63
19_A22_I0.8600.62
83_A192_A0.8450.61
201_E222_L0.8420.61
144_P155_N0.8400.60
135_I184_N0.8380.60
98_D105_L0.8370.60
28_S32_T0.8350.60
187_V198_T0.8310.60
142_I204_I0.8280.59
31_P192_A0.8260.59
303_D312_A0.8100.58
21_D56_V0.8050.57
247_K293_A0.8050.57
82_L192_A0.8030.57
275_A290_Y0.8000.57
216_L278_Y0.7990.57
244_V249_V0.7990.57
18_M22_I0.7980.56
92_A99_K0.7930.56
104_R259_E0.7910.56
65_G152_A0.7900.56
219_A222_L0.7890.56
22_I55_V0.7870.55
161_G305_E0.7870.55
82_L211_W0.7850.55
249_V252_K0.7850.55
161_G164_L0.7820.55
103_K296_K0.7820.55
32_T192_A0.7760.54
119_L218_A0.7750.54
142_I198_T0.7720.54
37_E201_E0.7700.54
83_A150_G0.7690.54
65_G151_G0.7680.54
182_Y188_L0.7670.54
311_K314_K0.7670.54
155_N209_I0.7670.54
287_A291_E0.7660.53
196_T200_V0.7640.53
210_A218_A0.7600.53
159_A181_L0.7590.53
181_L327_S0.7590.53
45_A50_Q0.7560.52
242_V265_L0.7560.52
29_Y81_T0.7510.52
235_S299_L0.7500.52
104_R244_V0.7500.52
178_V326_I0.7490.52
18_M53_D0.7480.52
151_G154_W0.7450.51
39_Y260_A0.7450.51
132_N177_F0.7420.51
174_A300_F0.7390.51
156_Y178_V0.7390.51
166_H173_K0.7380.51
162_Y234_E0.7370.51
163_A174_A0.7320.50
83_A151_G0.7320.50
189_D198_T0.7300.50
40_N261_Y0.7290.50
197_N200_V0.7280.50
25_L202_R0.7260.50
83_A154_W0.7220.49
65_G155_N0.7220.49
200_V222_L0.7200.49
140_S209_I0.7190.49
36_Y81_T0.7190.49
91_I95_G0.7180.49
105_L109_S0.7180.49
154_W211_W0.7130.48
151_G211_W0.7090.48
119_L196_T0.7080.48
211_W240_P0.7070.48
37_E66_K0.7070.48
35_L39_Y0.7040.47
182_Y326_I0.7040.47
284_A288_K0.7020.47
155_N192_A0.7010.47
287_A290_Y0.6980.47
301_T308_G0.6980.47
299_L302_I0.6980.47
143_T209_I0.6980.47
95_G98_D0.6950.47
164_L169_N0.6950.47
161_G234_E0.6940.46
286_V289_K0.6930.46
223_G226_K0.6890.46
126_K206_D0.6880.46
84_L211_W0.6880.46
51_T256_E0.6850.46
272_E291_E0.6840.46
323_F327_S0.6840.46
200_V264_Y0.6760.45
39_Y42_A0.6750.45
138_G198_T0.6730.44
162_Y167_N0.6720.44
285_E288_K0.6710.44
219_A229_I0.6670.44
82_L154_W0.6670.44
268_P272_E0.6640.44
112_Y274_A0.6640.44
171_Q174_A0.6620.43
188_L323_F0.6600.43
220_N276_K0.6600.43
151_G195_S0.6580.43
144_P189_D0.6570.43
246_D259_E0.6540.43
115_T235_S0.6540.43
247_K285_E0.6520.42
26_N40_N0.6470.42
195_S318_A0.6450.42
107_D283_D0.6450.42
276_K290_Y0.6430.42
200_V224_K0.6420.42
162_Y233_S0.6420.42
91_I94_R0.6420.42
164_L170_D0.6410.41
47_W260_A0.6400.41
31_P112_Y0.6400.41
14_A17_V0.6390.41
160_W174_A0.6380.41
231_T287_A0.6380.41
44_S55_V0.6370.41
172_A180_A0.6370.41
46_H260_A0.6370.41
37_E59_Q0.6350.41
75_I197_N0.6350.41
116_I209_I0.6260.40
80_V243_S0.6250.40
102_I303_D0.6250.40
219_A228_E0.6240.40
310_T314_K0.6240.40
143_T154_W0.6230.40
31_P240_P0.6230.40
17_V57_I0.6230.40
155_N211_W0.6230.40
188_L217_L0.6190.39
313_Q321_G0.6190.39
84_L155_N0.6180.39
178_V322_T0.6170.39
318_A321_G0.6160.39
168_N243_S0.6140.39
26_N81_T0.6140.39
23_Q252_K0.6140.39
145_N187_V0.6120.39
137_P226_K0.6110.39
132_N135_I0.6090.38
111_P242_V0.6080.38
37_E58_R0.6080.38
82_L152_A0.6080.38
95_G99_K0.6080.38
24_L57_I0.6070.38
203_G226_K0.6070.38
303_D306_F0.6050.38
32_T82_L0.6030.38
20_K325_Q0.6030.38
27_V62_G0.6020.38
137_P296_K0.6020.38
32_T83_A0.6010.38
224_K278_Y0.5970.37
163_A167_N0.5970.37
44_S257_V0.5970.37
115_T297_L0.5970.37
176_D184_N0.5960.37
110_A282_R0.5960.37
228_E321_G0.5950.37
162_Y166_H0.5950.37
109_S243_S0.5950.37
134_L185_V0.5940.37
75_I153_R0.5930.37
90_A94_R0.5930.37
299_L303_D0.5910.37
88_V110_A0.5910.37
145_N189_D0.5890.37
83_A91_I0.5890.37
84_L143_T0.5890.37
83_A152_A0.5880.37
305_E308_G0.5870.36
184_N195_S0.5800.36
201_E319_N0.5800.36
312_A322_T0.5790.36
116_I211_W0.5790.36
244_V262_L0.5780.36
64_S243_S0.5780.36
216_L229_I0.5770.36
16_S19_A0.5770.36
92_A243_S0.5740.35
32_T84_L0.5730.35
50_Q259_E0.5730.35
117_V229_I0.5710.35
86_Y313_Q0.5710.35
83_A240_P0.5710.35
89_D92_A0.5700.35
283_D288_K0.5700.35
120_V228_E0.5670.35
39_Y273_I0.5660.35
264_Y269_E0.5660.35
31_P83_A0.5640.34
211_W238_A0.5640.34
53_D257_V0.5620.34
30_D191_G0.5620.34
190_S194_G0.5610.34
32_T211_W0.5600.34
143_T211_W0.5550.34
153_R208_L0.5540.34
152_A231_T0.5530.33
113_T240_P0.5520.33
116_I155_N0.5520.33
164_L178_V0.5510.33
29_Y36_Y0.5490.33
78_D254_T0.5490.33
65_G211_W0.5490.33
145_N188_L0.5480.33
197_N312_A0.5450.33
200_V278_Y0.5440.33
63_G191_G0.5440.33
322_T325_Q0.5430.33
20_K53_D0.5430.33
189_D194_G0.5430.33
137_P289_K0.5410.32
292_N314_K0.5410.32
301_T305_E0.5410.32
300_F308_G0.5400.32
14_A18_M0.5380.32
41_K190_S0.5380.32
22_I96_R0.5380.32
56_V128_I0.5380.32
254_T257_V0.5380.32
244_V255_K0.5370.32
143_T151_G0.5370.32
129_H226_K0.5370.32
40_N61_H0.5360.32
310_T318_A0.5350.32
144_P197_N0.5340.32
85_A282_R0.5340.32
186_E295_P0.5310.32
192_A240_P0.5310.32
44_S267_S0.5290.31
26_N30_D0.5290.31
275_A281_P0.5280.31
187_V320_G0.5280.31
100_E171_Q0.5280.31
85_A88_V0.5270.31
153_R156_Y0.5260.31
247_K251_K0.5260.31
83_A155_N0.5250.31
82_L240_P0.5250.31
160_W316_H0.5250.31
270_G282_R0.5250.31
256_E260_A0.5240.31
65_G302_I0.5210.31
240_P279_Y0.5190.31
107_D285_E0.5190.31
49_Q103_K0.5190.31
83_A143_T0.5180.31
137_P288_K0.5160.30
157_L317_F0.5160.30
80_V91_I0.5160.30
55_V268_P0.5160.30
15_T18_M0.5150.30
71_V91_I0.5140.30
40_N59_Q0.5140.30
218_A227_F0.5130.30
17_V53_D0.5130.30
14_A19_A0.5130.30
15_T286_V0.5120.30
83_A116_I0.5120.30
31_P151_G0.5110.30
30_D81_T0.5100.30
129_H311_K0.5100.30
127_Q138_G0.5100.30
30_D33_R0.5090.30
16_S20_K0.5080.30
143_T240_P0.5060.30
239_E270_G0.5060.30
86_Y310_T0.5040.29
178_V306_F0.5040.29
161_G306_F0.5030.29
104_R109_S0.5030.29
92_A95_G0.5030.29
81_T111_P0.5020.29
143_T192_A0.5010.29
120_V261_Y0.5010.29
103_K108_N0.5000.29
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1sbpA 1 0.9392 100 0.603 Contact Map
4jb7A 2 0.693 100 0.801 Contact Map
4rxlA 1 0.6687 99.9 0.822 Contact Map
3r26A 1 0.6748 99.9 0.834 Contact Map
4g68A 1 0.9119 99.9 0.836 Contact Map
3cg1A 1 0.7325 99.9 0.839 Contact Map
2hxwA 2 0.6991 99.9 0.844 Contact Map
3rpwA 1 0.8571 99.9 0.851 Contact Map
4kd5C 3 0.6809 99.8 0.855 Contact Map
3k6vA 1 0.7842 99.8 0.855 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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