GREMLIN Database
RIBF - Riboflavin biosynthesis protein RibF
UniProt: P0AG40 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11079
Length: 313 (293)
Sequences: 3773 (2798)
Seq/√Len: 163.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
197_H200_E3.6021.00
227_V241_A3.1291.00
177_N180_L3.0371.00
179_A278_K2.8481.00
287_A291_E2.7511.00
279_K282_N2.7401.00
262_D304_R2.5651.00
79_L90_V2.4661.00
178_L182_E2.4411.00
229_V272_I2.3341.00
201_L249_V2.2441.00
233_G270_R2.1291.00
109_S114_K2.1241.00
242_N283_E2.1231.00
40_Q44_R2.0901.00
42_E45_K2.0791.00
144_G151_I2.0591.00
300_E304_R2.0211.00
38_G152_T2.0161.00
241_A258_V2.0071.00
227_V239_G1.9611.00
50_V88_D1.9531.00
228_E275_V1.9311.00
136_D139_L1.9081.00
229_V260_L1.9081.00
240_V296_I1.9011.00
120_F152_T1.8821.00
42_E46_R1.8771.00
38_G41_E1.8651.00
293_K297_A1.8351.00
200_E203_R1.8301.00
128_R136_D1.7941.00
240_V303_A1.7861.00
227_V258_V1.7851.00
228_E238_P1.7781.00
120_F150_D1.7661.00
106_N110_D1.7651.00
210_A267_L1.7571.00
144_G149_F1.7481.00
222_K254_Q1.7481.00
280_I302_T1.7441.00
240_V300_E1.7181.00
17_G119_K1.7081.00
108_I143_A1.6821.00
162_V168_A1.6811.00
228_E236_P1.6801.00
110_D114_K1.6641.00
105_Q109_S1.6571.00
20_L53_M1.6171.00
118_V121_L1.6061.00
263_V267_L1.6031.00
190_A273_Q1.6021.00
199_D255_Q1.5991.00
20_L118_V1.5951.00
229_V239_G1.5921.00
101_A131_A1.5871.00
53_M93_V1.5781.00
226_A306_F1.5671.00
105_Q146_E1.5631.00
280_I303_A1.5491.00
230_L275_V1.5451.00
159_E164_I1.5341.00
288_S291_E1.5261.00
207_F297_A1.5151.00
190_A275_V1.5081.00
125_D156_T1.5031.00
139_L143_A1.5021.00
22_I127_F1.4981.00
52_V87_V1.4951.00
228_E277_R1.4791.00
278_K306_F1.4611.00
43_G50_V1.4541.00
93_V99_F1.4451.00
18_C51_M1.4441.00
75_L92_C1.4421.00
51_M91_L1.4311.00
249_V289_L1.4261.00
40_Q50_V1.4261.00
172_A180_L1.4221.00
137_F153_S1.4131.00
210_A272_I1.4111.00
297_A300_E1.3931.00
230_L236_P1.3861.00
97_R101_A1.3841.00
109_S147_Y1.3811.00
73_T81_Y1.3771.00
230_L273_Q1.3481.00
239_G258_V1.3471.00
49_P89_Y1.3371.00
99_F102_L1.3301.00
103_T106_N1.3271.00
261_L304_R1.3271.00
302_T305_E1.3241.00
221_V279_K1.3171.00
277_R306_F1.3111.00
48_L119_K1.3111.00
261_L303_A1.3041.00
192_S271_H1.2961.00
201_L204_T1.2941.00
77_E217_Q1.2921.00
295_Q299_D1.2751.00
298_R301_L1.2621.00
192_S273_Q1.2581.00
172_A177_N1.2391.00
138_L141_Q1.2351.00
191_I276_L1.2341.00
199_D246_R1.2321.00
171_Q175_D1.2311.00
294_A298_R1.2211.00
231_G270_R1.2171.00
169_V184_L1.2001.00
260_L267_L1.1921.00
193_G212_V1.1801.00
238_P307_F1.1761.00
226_A277_R1.1741.00
243_I256_L1.1641.00
277_R307_F1.1621.00
64_A67_K1.1481.00
157_F164_I1.1471.00
224_V242_N1.1431.00
33_R37_Q1.1421.00
49_P88_D1.1351.00
298_R302_T1.1251.00
19_V122_A1.1241.00
168_A184_L1.1241.00
295_Q298_R1.1201.00
108_I149_F1.1141.00
39_L50_V1.1091.00
51_M89_Y1.0971.00
227_V276_L1.0931.00
34_A157_F1.0891.00
30_R159_E1.0881.00
77_E192_S1.0831.00
207_F300_E1.0790.99
50_V87_V1.0730.99
182_E188_P1.0700.99
80_R192_S1.0690.99
212_V258_V1.0680.99
188_P275_V1.0670.99
34_A37_Q1.0660.99
301_L305_E1.0530.99
113_V149_F1.0460.99
62_L96_D1.0440.99
246_R255_Q1.0420.99
19_V120_F1.0410.99
21_T39_L1.0410.99
128_R133_R1.0390.99
205_I289_L1.0330.99
36_L85_C1.0310.99
237_L260_L1.0230.99
16_E49_P1.0210.99
75_L79_L1.0170.99
245_T254_Q1.0170.99
54_L90_V1.0140.99
219_S243_I1.0140.99
33_R85_C1.0120.99
188_P277_R1.0100.99
195_V272_I1.0080.99
95_F100_A1.0060.99
290_D293_K1.0030.99
194_R269_G0.9980.99
104_A134_E0.9940.99
261_L300_E0.9860.99
81_Y187_H0.9750.99
242_N284_Q0.9670.99
289_L293_K0.9600.99
239_G261_L0.9570.99
238_P261_L0.9540.99
164_I184_L0.9530.99
142_K146_E0.9520.99
53_M91_L0.9510.99
97_R100_A0.9490.99
138_L142_K0.9430.99
105_Q134_E0.9430.99
178_L221_V0.9420.99
36_L52_V0.9380.99
139_L142_K0.9360.99
126_D163_R0.9330.99
70_A174_A0.9290.98
61_E216_R0.9280.98
222_K245_T0.9260.98
129_F139_L0.9250.98
102_L110_D0.9230.98
173_L178_L0.9220.98
125_D155_Q0.9200.98
104_A135_G0.9190.98
241_A256_L0.9180.98
52_V82_L0.9180.98
30_R186_G0.9160.98
297_A301_L0.9150.98
187_H190_A0.9090.98
108_I139_L0.9090.98
42_E120_F0.9070.98
36_L87_V0.9040.98
278_K302_T0.9010.98
37_Q41_E0.9000.98
122_A152_T0.8990.98
236_P275_V0.8970.98
30_R33_R0.8960.98
156_T163_R0.8940.98
194_R271_H0.8940.98
46_R150_D0.8900.98
221_V282_N0.8900.98
135_G139_L0.8900.98
290_D294_A0.8880.98
108_I113_V0.8840.98
231_G235_K0.8810.98
178_L181_A0.8790.98
168_A172_A0.8790.98
296_I300_E0.8770.98
259_H300_E0.8750.98
106_N109_S0.8700.98
80_R83_A0.8690.98
235_K265_M0.8690.98
19_V39_L0.8660.98
205_I296_I0.8540.97
199_D250_A0.8530.97
113_V147_Y0.8520.97
104_A139_L0.8510.97
56_E92_C0.8490.97
17_G117_R0.8470.97
231_G234_E0.8430.97
142_K145_M0.8390.97
24_N57_P0.8290.97
30_R164_I0.8270.97
129_F143_A0.8210.97
207_F296_I0.8190.97
82_L90_V0.8190.97
42_E122_A0.8180.97
259_H296_I0.8180.97
102_L106_N0.8130.96
201_L289_L0.8110.96
166_S170_R0.8060.96
62_L97_R0.8040.96
242_N293_K0.8000.96
42_E152_T0.8000.96
180_L183_S0.8000.96
245_T283_E0.7970.96
28_V82_L0.7940.96
123_V137_F0.7930.96
103_T134_E0.7920.96
63_F100_A0.7910.96
102_L111_L0.7900.96
258_V274_V0.7870.96
110_D115_H0.7850.96
18_C48_L0.7830.96
196_V213_P0.7800.96
81_Y84_E0.7790.96
37_Q40_Q0.7790.96
199_D249_V0.7790.96
231_G237_L0.7760.95
284_Q291_E0.7750.95
284_Q295_Q0.7750.95
222_K283_E0.7740.95
46_R120_F0.7730.95
141_Q145_M0.7720.95
240_V261_L0.7720.95
301_L304_R0.7710.95
182_E187_H0.7700.95
125_D163_R0.7690.95
267_L270_R0.7680.95
164_I169_V0.7670.95
243_I276_L0.7650.95
162_V171_Q0.7640.95
126_D156_T0.7620.95
281_R295_Q0.7600.95
213_P253_R0.7570.95
302_T306_F0.7560.95
160_G183_S0.7510.95
77_E190_A0.7510.95
159_E184_L0.7460.94
240_V259_H0.7440.94
228_E307_F0.7440.94
294_A297_A0.7420.94
172_A184_L0.7410.94
74_R217_Q0.7400.94
247_P283_E0.7360.94
60_L67_K0.7340.94
226_A307_F0.7290.94
19_V48_L0.7290.94
41_E45_K0.7290.94
178_L276_L0.7270.94
44_R88_D0.7270.94
134_E139_L0.7260.93
79_L83_A0.7260.93
28_V36_L0.7210.93
28_V52_V0.7200.93
260_L263_V0.7190.93
74_R77_E0.7170.93
33_R187_H0.7140.93
291_E294_A0.7130.93
159_E162_V0.7120.93
241_A276_L0.7120.93
72_L189_F0.7100.93
212_V272_I0.7090.93
46_R119_K0.7080.93
53_M99_F0.7070.93
231_G272_I0.7060.93
256_L276_L0.7050.92
24_N58_Q0.7010.92
226_A240_V0.7010.92
79_L92_C0.7000.92
38_G42_E0.6990.92
40_Q43_G0.6990.92
58_Q71_R0.6990.92
103_T131_A0.6980.92
19_V46_R0.6960.92
242_N246_R0.6900.92
231_G267_L0.6890.92
54_L92_C0.6880.92
173_L218_V0.6840.91
19_V42_E0.6830.91
220_P243_I0.6800.91
83_A90_V0.6770.91
240_V280_I0.6760.91
20_L51_M0.6750.91
63_F95_F0.6730.91
226_A303_A0.6700.90
39_L122_A0.6690.90
230_L234_E0.6690.90
77_E80_R0.6690.90
144_G148_G0.6680.90
131_A134_E0.6670.90
303_A307_F0.6670.90
249_V293_K0.6650.90
242_N247_P0.6570.90
24_N71_R0.6570.90
242_N259_H0.6570.90
266_D270_R0.6560.90
105_Q143_A0.6550.89
30_R157_F0.6520.89
36_L82_L0.6490.89
143_A146_E0.6480.89
222_K282_N0.6470.89
40_Q87_V0.6470.89
196_V255_Q0.6460.89
137_F141_Q0.6430.89
168_A180_L0.6420.89
159_E183_S0.6410.88
121_L151_I0.6400.88
127_F140_L0.6400.88
162_V180_L0.6360.88
159_E168_A0.6360.88
123_V140_L0.6350.88
199_D251_G0.6340.88
80_R273_Q0.6330.88
227_V274_V0.6320.88
112_L118_V0.6310.88
22_I53_M0.6290.88
76_R271_H0.6240.87
81_Y85_C0.6210.87
56_E75_L0.6190.87
210_A229_V0.6190.87
60_L64_A0.6190.87
61_E71_R0.6180.87
173_L220_P0.6150.86
176_D279_K0.6140.86
21_T50_V0.6140.86
281_R299_D0.6140.86
141_Q151_I0.6130.86
144_G147_Y0.6110.86
77_E273_Q0.6110.86
197_H269_G0.6090.86
248_T285_R0.6070.86
56_E94_R0.6070.86
80_R84_E0.6060.86
219_S256_L0.6030.85
76_R79_L0.6010.85
168_A171_Q0.5950.84
43_G46_R0.5930.84
21_T52_V0.5920.84
238_P262_D0.5920.84
284_Q287_A0.5910.84
57_P62_L0.5910.84
246_R283_E0.5900.84
44_R50_V0.5880.84
125_D153_S0.5830.83
121_L149_F0.5800.83
50_V82_L0.5790.83
17_G48_L0.5780.83
48_L120_F0.5770.83
174_A218_V0.5730.82
207_F242_N0.5720.82
231_G236_P0.5720.82
267_L272_I0.5720.82
108_I140_L0.5660.81
210_A260_L0.5660.81
15_Q18_C0.5660.81
213_P255_Q0.5660.81
40_Q85_C0.5640.81
140_L149_F0.5630.81
200_E204_T0.5620.81
104_A132_G0.5610.81
123_V133_R0.5600.81
73_T187_H0.5580.81
16_E117_R0.5570.80
291_E295_Q0.5540.80
96_D99_F0.5490.79
108_I144_G0.5490.79
18_C89_Y0.5470.79
60_L69_P0.5460.79
218_V225_Y0.5400.78
126_D133_R0.5370.78
98_R101_A0.5330.77
79_L82_L0.5320.77
217_Q220_P0.5310.77
123_V127_F0.5310.77
121_L140_L0.5300.77
54_L58_Q0.5290.77
171_Q174_A0.5290.77
110_D113_V0.5230.76
57_P75_L0.5220.76
125_D137_F0.5210.76
24_N126_D0.5200.76
18_C49_P0.5180.75
261_L307_F0.5170.75
57_P92_C0.5170.75
56_E71_R0.5150.75
33_R84_E0.5120.75
288_S294_A0.5100.74
127_F240_V0.5100.74
135_G140_L0.5100.74
35_L122_A0.5100.74
76_R192_S0.5090.74
291_E298_R0.5090.74
109_S146_E0.5090.74
237_L263_V0.5090.74
226_A278_K0.5080.74
21_T51_M0.5070.74
136_D142_K0.5070.74
60_L133_R0.5060.74
34_A38_G0.5060.74
41_E44_R0.5060.74
38_G122_A0.5060.74
28_V85_C0.5060.74
60_L63_F0.5040.73
20_L93_V0.5030.73
20_L121_L0.5020.73
55_F127_F0.5000.73
114_K305_E0.5000.73
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2x0kA 5 0.9936 100 0.179 Contact Map
3op1A 4 0.9617 100 0.213 Contact Map
1mrzA 2 0.853 100 0.225 Contact Map
3bnwA 2 0.3994 100 0.593 Contact Map
1n08A 1 0.4473 100 0.598 Contact Map
1nb0A 1 0.4121 100 0.606 Contact Map
3covA 2 0.7348 100 0.68 Contact Map
2ejcA 2 0.7476 100 0.709 Contact Map
3ag6A 2 0.754 100 0.717 Contact Map
3hl4A 2 0.4824 100 0.719 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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