GREMLIN Database
RISA - Riboflavin synthase
UniProt: P0AFU8 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11406
Length: 213 (196)
Sequences: 3742 (2517)
Seq/√Len: 179.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
89_K92_D3.1061.00
107_A123_F2.6021.00
70_I136_Y2.5191.00
122_W156_R2.3931.00
143_D174_K2.3191.00
108_E124_K2.3161.00
105_T130_L2.3131.00
55_N58_H2.3071.00
81_W173_K2.2541.00
134_I149_V2.1681.00
132_K192_V2.0911.00
53_E60_S2.0771.00
11_K27_E2.0701.00
88_A94_I2.0631.00
110_A124_K2.0611.00
13_V27_E2.0561.00
133_Y192_V2.0301.00
75_D179_R1.9921.00
122_W158_C1.9821.00
119_R166_L1.9661.00
153_T156_R1.9161.00
27_E57_N1.8951.00
11_K81_W1.8931.00
124_K155_T1.8701.00
25_V60_S1.8051.00
90_F98_L1.7961.00
174_K178_A1.7811.00
142_I180_V1.7761.00
145_I171_L1.7561.00
65_K69_R1.7301.00
164_E168_R1.7291.00
151_E158_C1.7041.00
10_A84_V1.6531.00
112_I121_I1.6451.00
36_L42_V1.6281.00
8_G28_L1.6201.00
37_E40_A1.6011.00
44_H82_V1.5981.00
115_S120_Q1.5951.00
108_E179_R1.5821.00
142_I182_I1.5721.00
125_V182_I1.5631.00
90_F94_I1.5571.00
64_M160_H1.5491.00
109_V121_I1.5301.00
112_I119_R1.5281.00
17_E22_R1.5171.00
109_V174_K1.5111.00
76_L80_D1.5001.00
47_C71_T1.4771.00
124_K156_R1.4761.00
25_V58_H1.4741.00
111_K122_W1.4511.00
7_Q86_R1.3981.00
109_V123_F1.3831.00
187_Q191_V1.3811.00
38_T164_E1.3701.00
99_M141_G1.3541.00
135_L188_T1.3381.00
118_N160_H1.3171.00
7_Q93_E1.3141.00
44_H59_V1.3131.00
145_I170_T1.2761.00
98_L186_P1.2651.00
134_I157_F1.2591.00
121_I166_L1.2561.00
120_Q158_C1.2461.00
66_E137_K1.2421.00
9_T81_W1.2411.00
94_I98_L1.2391.00
150_G158_C1.2321.00
45_N143_D1.2251.00
143_D170_T1.2211.00
134_I140_I1.2081.00
121_I161_L1.2051.00
77_K80_D1.2051.00
107_A142_I1.1901.00
166_L172_G1.1881.00
45_N72_N1.1871.00
27_E58_H1.1851.00
123_F159_V1.1671.00
150_G160_H1.1631.00
135_L193_D1.1501.00
98_L187_Q1.1411.00
135_L186_P1.1411.00
13_V58_H1.1361.00
72_N179_R1.1341.00
66_E70_I1.1311.00
125_V134_I1.1301.00
8_G31_H1.1271.00
142_I159_V1.1191.00
69_R115_S1.1161.00
52_T62_D1.1061.00
145_I161_L1.0981.00
36_L54_I1.0851.00
24_H63_L1.0831.00
45_N76_L1.0821.00
110_A156_R1.0811.00
171_L174_K1.0761.00
106_T179_R1.0731.00
145_I174_K1.0691.00
36_L51_V1.0641.00
86_R93_E1.0601.00
135_L189_Q1.0591.00
65_K115_S1.0551.00
133_Y184_I1.0541.00
23_T52_T1.0421.00
126_Q129_Q1.0421.00
15_I22_R1.0200.99
192_V196_E1.0130.99
114_T119_R1.0060.99
120_Q160_H0.9950.99
123_F180_V0.9930.99
33_L36_L0.9870.99
112_I175_K0.9720.99
51_V54_I0.9710.99
81_W172_G0.9690.99
107_A182_I0.9620.99
100_S186_P0.9510.99
136_Y151_E0.9480.99
10_A26_V0.9390.99
89_K94_I0.9360.99
109_V180_V0.9330.99
35_G93_E0.9280.99
140_I157_F0.9240.99
31_H34_D0.9230.99
9_T170_T0.9210.99
64_M150_G0.9170.99
21_F117_N0.9150.99
175_K178_A0.9130.99
35_G86_R0.9120.99
125_V131_M0.9050.99
40_A95_G0.9040.99
72_N181_N0.9030.99
120_Q150_G0.9000.99
121_I171_L0.8950.99
12_L82_V0.8890.99
87_A93_E0.8890.99
109_V112_I0.8870.99
16_D23_T0.8780.98
100_S103_I0.8770.98
14_S25_V0.8770.98
170_T174_K0.8740.98
14_S78_V0.8650.98
23_T60_S0.8590.98
161_L166_L0.8580.98
111_K176_L0.8550.98
8_G32_M0.8420.98
12_L15_I0.8370.98
21_F116_E0.8370.98
12_L26_V0.8360.98
142_I157_F0.8350.98
38_T54_I0.8310.98
125_V130_L0.8270.98
35_G95_G0.8240.98
22_R68_L0.8180.97
130_L184_I0.8180.97
88_A92_D0.8150.97
47_C103_I0.8060.97
12_L76_L0.8060.97
73_L76_L0.8010.97
125_V157_F0.7970.97
9_T168_R0.7940.97
62_D163_P0.7870.97
131_M193_D0.7850.97
105_T125_V0.7830.97
193_D197_R0.7800.97
40_A93_E0.7620.96
18_K23_T0.7580.96
112_I176_L0.7560.96
32_M35_G0.7440.96
118_N166_L0.7430.96
145_I169_T0.7410.95
64_M118_N0.7360.95
105_T126_Q0.7340.95
49_L67_T0.7300.95
188_T194_T0.7240.95
157_F182_I0.7240.95
23_T53_E0.7190.95
140_I182_I0.7190.95
26_V84_V0.7140.94
187_Q195_V0.7140.94
25_V55_N0.7110.94
131_M155_T0.7100.94
74_G179_R0.7050.94
44_H84_V0.7040.94
23_T62_D0.7000.94
152_V193_D0.6950.94
28_L32_M0.6880.93
68_L74_G0.6880.93
174_K180_V0.6870.93
54_I58_H0.6840.93
132_K194_T0.6840.93
36_L86_R0.6840.93
38_T52_T0.6830.93
103_I141_G0.6800.93
28_L33_L0.6780.93
5_I95_G0.6780.93
26_V61_F0.6770.93
113_L120_Q0.6740.93
188_T191_V0.6730.93
32_M36_L0.6730.93
128_S154_P0.6720.93
113_L122_W0.6710.92
45_N170_T0.6670.92
7_Q92_D0.6600.92
30_D33_L0.6530.91
184_I189_Q0.6510.91
50_T164_E0.6490.91
191_V195_V0.6490.91
63_L67_T0.6440.91
20_N65_K0.6430.91
135_L190_A0.6370.90
89_K93_E0.6350.90
15_I78_V0.6330.90
122_W153_T0.6290.90
107_A125_V0.6260.90
133_Y193_D0.6250.90
22_R65_K0.6230.89
49_L63_L0.6220.89
3_T94_I0.6180.89
13_V78_V0.6160.89
13_V25_V0.6150.89
128_S155_T0.6140.89
44_H76_L0.6130.89
110_A176_L0.6120.89
40_A87_A0.6120.89
112_I166_L0.6100.88
45_N80_D0.6080.88
64_M159_V0.6070.88
37_E54_I0.6070.88
9_T172_G0.6070.88
113_L158_C0.6050.88
36_L40_A0.6040.88
18_K21_F0.6010.88
72_N143_D0.6000.88
1_M4_G0.5970.87
73_L82_V0.5910.87
123_F142_I0.5880.87
102_H139_F0.5850.86
13_V57_N0.5840.86
38_T51_V0.5810.86
73_L80_D0.5800.86
115_S118_N0.5780.86
26_V44_H0.5780.86
38_T168_R0.5770.86
33_L59_V0.5760.85
40_A86_R0.5750.85
195_V198_V0.5720.85
139_F145_I0.5720.85
193_D196_E0.5700.85
5_I87_A0.5660.84
68_L73_L0.5640.84
70_I150_G0.5630.84
190_A194_T0.5630.84
42_V54_I0.5610.84
65_K160_H0.5600.84
134_I182_I0.5590.84
123_F157_F0.5580.84
161_L171_L0.5570.84
139_F187_Q0.5560.83
120_Q151_E0.5560.83
161_L165_T0.5550.83
134_I184_I0.5540.83
139_F169_T0.5450.82
112_I172_G0.5450.82
44_H49_L0.5430.82
87_A100_S0.5390.81
98_L139_F0.5380.81
32_M86_R0.5380.81
184_I188_T0.5350.81
5_I32_M0.5340.81
110_A122_W0.5340.81
88_A141_G0.5320.81
30_D57_N0.5310.81
109_V176_L0.5270.80
105_T184_I0.5200.79
132_K197_R0.5200.79
145_I172_G0.5180.79
58_H61_F0.5170.79
97_H146_S0.5150.79
139_F163_P0.5120.78
99_M104_M0.5090.78
83_N170_T0.5090.78
44_H61_F0.5090.78
66_E69_R0.5070.77
38_T53_E0.5070.77
131_M152_V0.5060.77
53_E58_H0.5050.77
12_L73_L0.5050.77
70_I137_K0.5000.76
111_K156_R0.5000.76
64_M117_N0.5000.76
81_W111_K0.5000.76
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1i8dA 3 0.9484 100 0.076 Contact Map
1kzlA 1 0.9437 100 0.086 Contact Map
4g6iA 3 0.9202 100 0.091 Contact Map
3a35A 1 0.8638 100 0.138 Contact Map
3ddyA 1 0.8216 100 0.151 Contact Map
4tkoB 1 0.5915 45.3 0.954 Contact Map
2wg5A 3 0.2629 26.5 0.96 Contact Map
3h43A 5 0.277 19.8 0.962 Contact Map
2qcpX 1 0.3662 17 0.963 Contact Map
4nbqA 3 0.4413 15.9 0.964 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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