GREMLIN Database
FOLM - Dihydrofolate reductase FolM
UniProt: P0AFS3 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13189
Length: 240 (218)
Sequences: 131297 (91890)
Seq/√Len: 6223.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
103_A111_N3.2701.00
103_A107_Q2.9271.00
157_A161_N2.8351.00
61_T64_G2.8281.00
136_H163_T2.8141.00
133_D175_K2.5341.00
73_L123_L2.5151.00
9_I81_A2.4691.00
84_H136_H2.4001.00
20_L24_W2.3701.00
138_T160_D2.3191.00
66_M119_A2.1411.00
32_P51_G2.1381.00
117_N166_F2.1171.00
36_S57_A2.1061.00
81_A221_L2.0471.00
24_W50_A2.0081.00
185_L209_G2.0051.00
69_A120_L1.9951.00
15_G37_Y1.9361.00
107_Q111_N1.9291.00
121_E170_L1.9211.00
25_H29_Q1.9051.00
62_N99_A1.8761.00
8_P32_P1.8601.00
18_I184_I1.8491.00
14_G35_V1.8011.00
25_H215_D1.8001.00
34_I76_T1.7641.00
53_Q75_S1.7541.00
109_H155_S1.7341.00
39_T42_P1.7301.00
36_S68_F1.7251.00
71_E75_S1.7001.00
163_T178_S1.6791.00
40_H56_Q1.6791.00
22_L217_V1.6491.00
25_H218_D1.6101.00
83_L137_F1.6061.00
6_P31_Q1.6051.00
113_P159_L1.5901.00
70_D74_K1.5661.00
34_I72_V1.5551.00
208_P225_F1.5501.00
57_A64_G1.5401.00
26_F221_L1.5101.00
133_D177_N1.5091.00
106_M110_V1.4991.00
98_L101_V1.4931.00
25_H28_N1.4741.00
8_P78_G1.4601.00
44_I54_C1.4511.00
99_A115_L1.4491.00
55_I71_E1.4311.00
31_Q81_A1.4271.00
82_I116_L1.4101.00
23_A35_V1.4081.00
16_R187_N1.3881.00
45_D49_N1.3791.00
29_Q218_D1.3791.00
62_N115_L1.3791.00
66_M70_D1.3751.00
67_A71_E1.3721.00
84_H117_N1.3651.00
110_V158_A1.3651.00
123_L127_H1.3511.00
35_V52_A1.3511.00
57_A68_F1.3451.00
9_I31_Q1.3401.00
71_E74_K1.3361.00
55_I68_F1.3271.00
138_T178_S1.3111.00
32_P53_Q1.3101.00
65_V116_L1.3051.00
90_M104_C1.3051.00
117_N134_I1.2941.00
45_D48_I1.2861.00
62_N66_M1.2711.00
40_H45_D1.2671.00
9_I26_F1.2661.00
95_G125_R1.2621.00
8_P34_I1.2611.00
20_L50_A1.2481.00
20_L47_L1.2301.00
111_N115_L1.2291.00
64_G67_A1.2201.00
53_Q76_T1.2181.00
21_A24_W1.2001.00
20_L46_G1.1961.00
163_T176_V1.1941.00
78_G127_H1.1891.00
184_I208_P1.1571.00
192_A196_Q1.1571.00
16_R42_P1.1541.00
92_E101_V1.1541.00
10_L68_F1.1401.00
46_G50_A1.1391.00
44_I47_L1.1371.00
179_I217_V1.1361.00
106_M111_N1.1271.00
24_W28_N1.1171.00
185_L190_D1.1141.00
90_M101_V1.1081.00
85_N137_F1.1011.00
114_Y162_M1.0971.00
34_I53_Q1.0971.00
82_I120_L1.0941.00
99_A111_N1.0851.00
40_H54_C1.0831.00
88_A108_I1.0781.00
26_F31_Q1.0671.00
21_A211_K1.0641.00
7_L80_R1.0571.00
79_L120_L1.0531.00
196_Q200_N1.0291.00
63_D67_A1.0121.00
143_E180_A1.0101.00
60_S104_C1.0051.00
166_F176_V1.0051.00
34_I55_I0.9991.00
100_D104_C0.9961.00
117_N162_M0.9781.00
55_I75_S0.9771.00
44_I48_I0.9741.00
67_A70_D0.9591.00
21_A25_H0.9361.00
102_L111_N0.9251.00
219_Y223_S0.9251.00
25_H214_I0.9231.00
8_P76_T0.9191.00
142_V160_D0.9171.00
113_P136_H0.9121.00
22_L83_L0.9121.00
152_Y156_K0.9061.00
137_F217_V0.9041.00
97_P151_A0.9001.00
109_H156_K0.8951.00
88_A104_C0.8941.00
62_N118_H0.8941.00
59_F112_T0.8941.00
93_K96_A0.8921.00
187_N190_D0.8891.00
56_Q64_G0.8851.00
60_S107_Q0.8811.00
150_I169_K0.8801.00
27_I51_G0.8781.00
27_I52_A0.8771.00
10_L36_S0.8721.00
18_I22_L0.8671.00
124_L127_H0.8661.00
72_V76_T0.8631.00
106_M154_A0.8581.00
196_Q199_L0.8551.00
153_A161_N0.8551.00
160_D180_A0.8471.00
16_R20_L0.8461.00
99_A118_H0.8461.00
183_L189_H0.8431.00
117_N136_H0.8431.00
25_H211_K0.8371.00
57_A65_V0.8361.00
63_D66_M0.8361.00
148_K169_K0.8281.00
160_D178_S0.8261.00
46_G49_N0.8201.00
101_V104_C0.8151.00
73_L126_G0.8151.00
16_R39_T0.8121.00
123_L126_G0.8121.00
89_W188_E0.8111.00
69_A119_A0.8071.00
139_D152_Y0.8061.00
88_A188_E0.8041.00
127_H131_A0.8041.00
82_I134_I0.8021.00
10_L79_L0.8011.00
22_L214_I0.7981.00
206_T212_E0.7981.00
48_I54_C0.7931.00
26_F218_D0.7921.00
195_R199_L0.7791.00
32_P76_T0.7791.00
216_L219_Y0.7671.00
84_H116_L0.7591.00
53_Q71_E0.7591.00
211_K215_D0.7581.00
197_Q200_N0.7571.00
113_P117_N0.7561.00
125_R170_L0.7561.00
177_N220_L0.7531.00
99_A103_A0.7501.00
102_L151_A0.7461.00
133_D224_C0.7451.00
95_G170_L0.7431.00
145_G161_N0.7411.00
37_Y54_C0.7381.00
202_S225_F0.7331.00
153_A157_A0.7281.00
65_V115_L0.7271.00
106_M155_S0.7261.00
21_A214_I0.7241.00
37_Y56_Q0.7241.00
192_A195_R0.7231.00
80_R127_H0.7151.00
83_L217_V0.7141.00
79_L123_L0.7121.00
105_M155_S0.7071.00
14_G36_S0.7061.00
12_T85_N0.7041.00
124_L132_S0.7041.00
119_A122_R0.7011.00
60_S108_I0.6901.00
191_D194_Y0.6901.00
62_N119_A0.6881.00
209_G212_E0.6881.00
214_I218_D0.6861.00
138_T180_A0.6851.00
21_A210_E0.6821.00
135_I221_L0.6771.00
29_Q222_T0.6771.00
102_L114_Y0.6741.00
17_R186_F0.6721.00
41_Y45_D0.6721.00
73_L78_G0.6691.00
37_Y40_H0.6631.00
65_V119_A0.6621.00
94_P150_I0.6621.00
141_V153_A0.6611.00
165_S169_K0.6571.00
143_E160_D0.6561.00
47_L50_A0.6561.00
24_W27_I0.6531.00
110_V154_A0.6521.00
88_A155_S0.6511.00
136_H176_V0.6431.00
157_A160_D0.6411.00
131_A175_K0.6391.00
185_L195_R0.6361.00
12_T84_H0.6361.00
35_V54_C0.6351.00
69_A116_L0.6351.00
70_D73_L0.6351.00
12_T116_L0.6301.00
70_D123_L0.6291.00
134_I176_V0.6291.00
193_E196_Q0.6251.00
115_L119_A0.6251.00
121_E125_R0.6241.00
120_L124_L0.6231.00
170_L176_V0.6211.00
62_N100_D0.6191.00
94_P170_L0.6141.00
135_I217_V0.6141.00
127_H132_S0.6111.00
7_L31_Q0.6111.00
26_F81_A0.6071.00
113_P116_L0.6061.00
219_Y222_T0.6051.00
11_I23_A0.5991.00
150_I165_S0.5991.00
16_R37_Y0.5951.00
94_P166_F0.5921.00
212_E225_F0.5911.00
111_N114_Y0.5891.00
104_C107_Q0.5861.00
72_V77_H0.5861.00
37_Y44_I0.5851.00
78_G123_L0.5841.00
103_A115_L0.5841.00
157_A164_R0.5831.00
142_V157_A0.5821.00
160_D164_R0.5791.00
72_V75_S0.5771.00
167_A177_N0.5721.00
38_R58_D0.5681.00
48_I52_A0.5681.00
215_D224_C0.5661.00
9_I221_L0.5661.00
150_I162_M0.5661.00
164_R167_A0.5661.00
73_L77_H0.5641.00
212_E224_C0.5641.00
62_N103_A0.5631.00
110_V159_L0.5621.00
114_Y118_H0.5621.00
184_I213_V0.5621.00
55_I72_V0.5611.00
37_Y42_P0.5611.00
14_G23_A0.5591.00
69_A73_L0.5581.00
59_F86_A0.5551.00
87_S109_H0.5511.00
114_Y158_A0.5511.00
7_L131_A0.5451.00
24_W211_K0.5441.00
15_G38_R0.5441.00
12_T86_A0.5441.00
161_N164_R0.5411.00
108_I112_T0.5401.00
170_L174_V0.5401.00
26_F217_V0.5381.00
68_F116_L0.5371.00
70_D119_A0.5331.00
127_H130_A0.5291.00
10_L116_L0.5271.00
154_A162_M0.5211.00
161_N165_S0.5201.00
94_P162_M0.5191.00
206_T224_C0.5181.00
8_P72_V0.5181.00
6_P32_P0.5181.00
51_G75_S0.5161.00
42_P46_G0.5151.00
124_L131_A0.5121.00
90_M105_M0.5121.00
36_S55_I0.5101.00
38_R56_Q0.5101.00
171_A204_M0.5091.00
84_H113_P0.5081.00
212_E215_D0.5071.00
36_S59_F0.5061.00
23_A27_I0.5031.00
105_M151_A0.5031.00
10_L72_V0.5021.00
145_G157_A0.5021.00
27_I33_V0.5001.00
92_E96_A0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3gemA 4 0.9625 100 0.125 Contact Map
3wtcA 4 0.9875 100 0.136 Contact Map
2o23A 3 0.9708 100 0.141 Contact Map
1vl8A 4 0.9875 100 0.142 Contact Map
4weoA 4 0.9542 100 0.142 Contact Map
3pk0A 4 0.9875 100 0.142 Contact Map
4rgbA 2 0.9875 100 0.143 Contact Map
3rihA 4 0.9875 100 0.143 Contact Map
3lf2A 4 0.9792 100 0.144 Contact Map
1iy8A 4 0.9792 100 0.146 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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