GREMLIN Database
PROX - Glycine betaine-binding periplasmic protein
UniProt: P0AFM2 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10773
Length: 330 (274)
Sequences: 5199 (3720)
Seq/√Len: 224.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
121_K223_D4.8141.00
292_I301_G3.9541.00
121_K225_V3.8051.00
121_K125_D3.7031.00
282_P285_D3.3581.00
133_A230_P3.1671.00
104_Y263_K3.0001.00
308_D312_K2.8101.00
34_V79_A2.7961.00
130_T225_V2.7381.00
19_T22_A2.7001.00
50_R308_D2.5971.00
123_T127_Y2.5461.00
292_I309_G2.5041.00
118_L208_T2.4411.00
105_R258_H2.4201.00
30_T58_T2.3861.00
119_I129_I2.3441.00
309_G313_A2.2551.00
32_N60_N2.1481.00
131_N228_Q2.1051.00
30_T60_N2.0951.00
130_T134_Q2.0501.00
117_Y205_F2.0321.00
17_T20_F1.9821.00
292_I296_G1.9591.00
285_D289_Q1.8971.00
124_A225_V1.8941.00
43_F293_M1.8781.00
33_P81_F1.8731.00
49_S53_E1.8661.00
50_R53_E1.8481.00
263_K267_E1.8421.00
48_V83_A1.8091.00
92_N96_A1.8071.00
46_L50_R1.7841.00
120_D123_T1.7791.00
13_T16_S1.7611.00
301_G309_G1.7481.00
55_L275_L1.7091.00
72_T75_A1.6791.00
216_N288_A1.6611.00
131_N230_P1.6521.00
49_S62_P1.6311.00
140_I143_L1.6291.00
122_K201_G1.6151.00
195_I218_L1.6131.00
95_E99_G1.6011.00
173_A233_A1.5991.00
91_D95_E1.5841.00
130_T228_Q1.5331.00
16_S19_T1.5171.00
53_E59_V1.5151.00
302_D305_G1.5091.00
157_C163_C1.5081.00
51_A275_L1.4971.00
12_A15_I1.4961.00
91_D94_Y1.4891.00
104_Y266_A1.4801.00
301_G305_G1.4801.00
102_K263_K1.4711.00
103_F260_V1.4591.00
18_Q21_A1.4531.00
135_L205_F1.4521.00
266_A273_A1.4451.00
291_A295_D1.4391.00
195_I199_K1.4021.00
50_R304_Q1.3951.00
265_W269_N1.3921.00
49_S59_V1.3891.00
203_P206_Y1.3471.00
296_G301_G1.3461.00
143_L203_P1.3451.00
291_A296_G1.3351.00
116_G226_W1.3181.00
35_Q45_T1.2981.00
120_D201_G1.2891.00
293_M306_H1.2891.00
107_G277_A1.2881.00
46_L49_S1.2841.00
93_M97_A1.2731.00
90_H93_M1.2621.00
104_Y273_A1.2561.00
173_A231_F1.2461.00
123_T203_P1.2391.00
187_Y191_M1.2211.00
288_A292_I1.2111.00
46_L304_Q1.2091.00
305_G309_G1.2031.00
14_L17_T1.1961.00
129_I140_I1.1961.00
186_N190_M1.1881.00
153_D183_N1.1831.00
11_F14_L1.1791.00
186_N189_A1.1751.00
71_Y101_K1.1731.00
48_V275_L1.1671.00
274_K278_I1.1581.00
80_T262_N1.1561.00
17_T21_A1.1471.00
274_K277_A1.1461.00
51_A279_M1.1441.00
117_Y167_I1.1411.00
187_Y190_M1.1331.00
196_S200_E1.1301.00
73_S79_A1.1271.00
281_L289_Q1.1261.00
167_I170_Q1.1261.00
216_N287_N1.1171.00
108_V258_H1.1171.00
32_N63_S1.1161.00
112_G283_V1.1111.00
15_I20_F1.1051.00
303_I307_V1.0971.00
106_E261_A1.0951.00
18_Q22_A1.0911.00
160_G164_E1.0881.00
123_T144_F1.0831.00
50_R311_I1.0661.00
133_A231_F1.0641.00
34_V70_G1.0631.00
123_T129_I1.0581.00
186_N192_A1.0481.00
140_I144_F1.0471.00
31_V57_Y1.0391.00
259_I276_F1.0371.00
262_N268_K1.0361.00
174_Y229_V1.0341.00
90_H97_A1.0311.00
119_I123_T1.0271.00
141_A144_F1.0271.00
119_I124_A1.0251.00
170_Q233_A1.0241.00
284_A287_N1.0221.00
93_M96_A1.0211.00
71_Y75_A1.0201.00
15_I18_Q1.0181.00
261_A266_A1.0181.00
10_A13_T1.0161.00
47_L307_V1.0111.00
54_K278_I1.0091.00
85_N260_V1.0071.00
217_E295_D0.9981.00
52_L272_A0.9961.00
283_V287_N0.9911.00
289_Q306_H0.9841.00
52_L57_Y0.9821.00
161_W164_E0.9731.00
114_A210_T0.9721.00
19_T23_D0.9691.00
208_T214_V0.9651.00
309_G312_K0.9641.00
285_D310_W0.9611.00
285_D314_H0.9561.00
272_A276_F0.9561.00
116_G132_I0.9511.00
43_F289_Q0.9491.00
273_A277_A0.9461.00
167_I171_L0.9401.00
40_E286_I0.9341.00
106_E273_A0.9321.00
185_G189_A0.9321.00
123_T126_Q0.9291.00
285_D288_A0.9291.00
264_A268_K0.9281.00
94_Y97_A0.9251.00
47_L50_R0.9211.00
116_G210_T0.9170.99
136_K175_E0.9170.99
40_E84_V0.9140.99
157_C164_E0.9010.99
127_Y140_I0.9000.99
31_V269_N0.8990.99
155_T160_G0.8950.99
32_N80_T0.8930.99
12_A16_S0.8930.99
96_A99_G0.8860.99
265_W272_A0.8840.99
194_T206_Y0.8830.99
45_T62_P0.8820.99
169_H173_A0.8800.99
41_E84_V0.8760.99
168_N172_A0.8760.99
47_L311_I0.8750.99
157_C160_G0.8750.99
47_L281_L0.8740.99
94_Y98_G0.8690.99
189_A192_A0.8680.99
141_A152_A0.8680.99
60_N63_S0.8610.99
47_L310_W0.8600.99
193_D197_R0.8600.99
304_Q308_D0.8590.99
77_G80_T0.8590.99
51_A311_I0.8560.99
92_N95_E0.8530.99
122_K126_Q0.8490.99
39_T294_H0.8440.99
42_T303_I0.8430.99
47_L51_A0.8380.99
96_A100_D0.8370.99
73_S78_D0.8360.99
187_Y192_A0.8360.99
81_F276_F0.8350.99
129_I134_Q0.8330.99
153_D157_C0.8260.99
275_L279_M0.8250.99
81_F261_A0.8190.99
90_H94_Y0.8150.99
195_I217_E0.8140.99
301_G306_H0.8090.99
188_A191_M0.8080.99
29_I269_N0.8030.99
123_T140_I0.8020.99
284_A288_A0.8010.99
185_G188_A0.7970.99
81_F272_A0.7920.99
39_T293_M0.7910.99
136_K141_A0.7890.98
171_L182_H0.7880.98
124_A129_I0.7870.98
31_V272_A0.7870.98
118_L226_W0.7840.98
53_E58_T0.7840.98
48_V276_F0.7840.98
91_D98_G0.7790.98
111_N257_M0.7780.98
106_E277_A0.7760.98
43_F47_L0.7740.98
57_Y271_A0.7720.98
305_G308_D0.7720.98
72_T76_S0.7700.98
23_D26_G0.7650.98
70_G82_T0.7650.98
32_N79_A0.7560.98
185_G190_M0.7560.98
281_L286_I0.7530.98
9_T13_T0.7510.98
164_E184_Q0.7480.98
261_A265_W0.7420.98
15_I19_T0.7380.98
33_P45_T0.7360.98
120_D204_V0.7340.98
108_V256_T0.7310.98
16_S20_F0.7290.97
113_A255_S0.7250.97
48_V259_I0.7240.97
90_H98_G0.7230.97
20_F23_D0.7220.97
280_Q310_W0.7170.97
210_T287_N0.7170.97
169_H172_A0.7160.97
160_G163_C0.7090.97
186_N191_M0.7090.97
166_A169_H0.7080.97
48_V279_M0.7070.97
122_K198_Y0.7050.97
14_L18_Q0.7050.97
14_L19_T0.7010.97
95_E98_G0.6990.97
17_T22_A0.6960.97
158_N187_Y0.6930.97
52_L55_L0.6930.97
217_E291_A0.6920.97
154_L159_P0.6920.97
154_L157_C0.6890.96
197_R202_K0.6880.96
47_L289_Q0.6860.96
45_T83_A0.6860.96
132_I227_L0.6840.96
40_E44_Q0.6810.96
106_E259_I0.6750.96
133_A136_K0.6740.96
201_G204_V0.6730.96
47_L279_M0.6720.96
37_T209_W0.6700.96
170_Q229_V0.6700.96
46_L62_P0.6670.96
111_N286_I0.6640.96
213_W294_H0.6590.95
193_D204_V0.6590.95
71_Y85_N0.6580.95
117_Y132_I0.6560.95
262_N265_W0.6560.95
115_Q163_C0.6550.95
172_A177_T0.6490.95
36_S70_G0.6440.95
50_R307_V0.6420.95
163_C233_A0.6420.95
272_A275_L0.6360.94
155_T161_W0.6350.94
36_S82_T0.6320.94
136_K176_L0.6290.94
188_A192_A0.6260.94
37_T64_E0.6260.94
154_L161_W0.6250.94
93_M98_G0.6250.94
13_T17_T0.6250.94
180_V183_N0.6240.94
135_L227_L0.6230.94
159_P164_E0.6220.94
210_T226_W0.6220.94
165_G169_H0.6200.94
271_A274_K0.6200.94
192_A195_I0.6180.94
75_A102_K0.6160.93
14_L27_K0.6140.93
93_M165_G0.6100.93
288_A291_A0.6090.93
33_P52_L0.6090.93
292_I306_H0.6070.93
132_I176_L0.6060.93
215_S226_W0.6050.93
144_F205_F0.6050.93
185_G191_M0.6040.93
129_I135_L0.6040.93
159_P163_C0.6040.93
65_V70_G0.6030.93
125_D225_V0.6020.93
9_T16_S0.6000.93
155_T193_D0.5970.92
22_A25_P0.5970.92
216_N291_A0.5950.92
48_V109_F0.5900.92
25_P28_G0.5890.92
44_Q286_I0.5880.92
292_I313_A0.5870.92
118_L224_V0.5870.92
109_F281_L0.5860.92
44_Q83_A0.5850.92
129_I144_F0.5840.92
35_Q64_E0.5840.92
261_A273_A0.5820.91
24_L28_G0.5810.91
36_S84_V0.5790.91
62_P65_V0.5790.91
210_T283_V0.5770.91
50_R54_K0.5760.91
155_T185_G0.5760.91
104_Y264_A0.5730.91
91_D97_A0.5710.91
104_Y261_A0.5710.91
208_T215_S0.5690.91
134_Q227_L0.5690.91
18_Q23_D0.5680.90
74_L103_F0.5680.90
191_M194_T0.5680.90
119_I127_Y0.5670.90
116_G215_S0.5660.90
175_E178_N0.5630.90
40_E211_P0.5620.90
57_Y269_N0.5620.90
154_L160_G0.5610.90
190_M193_D0.5590.90
35_Q62_P0.5590.90
264_A267_E0.5580.90
196_S199_K0.5570.90
74_L262_N0.5560.89
154_L167_I0.5540.89
51_A54_K0.5540.89
7_F10_A0.5520.89
109_F259_I0.5520.89
11_F15_I0.5510.89
135_L144_F0.5510.89
7_F11_F0.5500.89
35_Q42_T0.5490.89
185_G193_D0.5460.89
31_V265_W0.5450.89
266_A272_A0.5440.88
292_I305_G0.5440.88
29_I57_Y0.5430.88
141_A179_T0.5410.88
11_F16_S0.5410.88
232_S253_P0.5400.88
9_T12_A0.5400.88
265_W273_A0.5390.88
174_Y231_F0.5350.88
191_M214_V0.5350.88
184_Q189_A0.5340.88
257_M286_I0.5330.87
12_A18_Q0.5330.87
39_T187_Y0.5320.87
118_L215_S0.5310.87
71_Y260_V0.5290.87
117_Y154_L0.5290.87
36_S41_E0.5250.87
54_K312_K0.5240.87
124_A130_T0.5220.86
218_L224_V0.5220.86
142_K145_D0.5220.86
134_Q139_K0.5200.86
37_T66_D0.5200.86
111_N256_T0.5200.86
157_C161_W0.5200.86
89_L105_R0.5200.86
10_A17_T0.5190.86
13_T19_T0.5180.86
155_T164_E0.5170.86
154_L164_E0.5150.86
216_N284_A0.5130.85
115_Q255_S0.5120.85
156_G164_E0.5080.85
35_Q41_E0.5070.85
131_N136_K0.5040.84
155_T159_P0.5030.84
125_D228_Q0.5030.84
71_Y103_F0.5030.84
33_P48_V0.5010.84
122_K313_A0.5010.84
55_L271_A0.5000.84
88_P255_S0.5000.84
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1r9lA 1 0.9364 100 0.324 Contact Map
3o66A 1 0.7545 100 0.427 Contact Map
4ne4A 1 0.7788 100 0.43 Contact Map
2rinA 1 0.8273 100 0.485 Contact Map
3r6uA 1 0.7515 100 0.49 Contact Map
1sw5A 1 0.7485 100 0.516 Contact Map
3tmgA 1 0.803 100 0.535 Contact Map
3l6gA 1 0.7727 100 0.538 Contact Map
2b4lA 1 0.497 100 0.541 Contact Map
1p99A 1 0.7091 99.6 0.792 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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