GREMLIN Database
POTB - Spermidine/putrescine transport system permease protein PotB
UniProt: P0AFK4 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10750
Length: 275 (245)
Sequences: 70635 (45560)
Seq/√Len: 2910.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
183_Q187_R4.0691.00
56_L60_N3.3131.00
175_R184_T3.0981.00
160_Y164_E2.9961.00
24_S43_Y2.8131.00
54_V229_V2.7481.00
162_S196_G2.6371.00
174_A184_T2.6261.00
53_E56_L2.6141.00
82_K171_L2.6041.00
64_I144_I2.6001.00
171_L189_I2.5841.00
180_S183_Q2.5781.00
68_A148_V2.5031.00
224_L232_V2.4611.00
41_D44_T2.3731.00
66_T201_C2.3631.00
181_K184_T2.3611.00
52_F56_L2.3601.00
182_L186_I2.3091.00
41_D45_R2.2611.00
159_L197_I2.2501.00
239_D242_F2.2331.00
179_A183_Q2.1161.00
183_Q186_I2.0381.00
74_Y185_F1.9991.00
155_M200_G1.9641.00
81_A164_E1.9591.00
170_L192_L1.9211.00
92_L160_Y1.9181.00
163_I193_T1.9101.00
22_G26_L1.9091.00
85_H88_R1.8631.00
174_A179_A1.8511.00
74_Y193_T1.8431.00
70_L201_C1.8221.00
171_L184_T1.7601.00
53_E57_H1.7541.00
175_R181_K1.7521.00
18_L22_G1.7441.00
62_A205_M1.7271.00
259_L263_R1.6631.00
171_L175_R1.6501.00
73_G155_M1.6311.00
65_A208_A1.6311.00
60_N64_I1.6231.00
74_Y159_L1.5991.00
80_L156_V1.5961.00
45_R242_F1.5961.00
76_F156_V1.5931.00
15_L19_M1.5271.00
155_M204_V1.5131.00
168_K171_L1.5071.00
229_V233_Q1.5031.00
71_V75_P1.4881.00
21_I248_I1.4851.00
87_V90_L1.4621.00
61_M225_L1.4541.00
212_F216_D1.4511.00
140_P223_N1.4231.00
40_L44_T1.4181.00
81_A160_Y1.4141.00
190_I197_I1.4061.00
60_N140_P1.3881.00
77_A159_L1.3681.00
199_A203_L1.3651.00
163_I189_I1.3651.00
39_T42_N1.3511.00
84_P88_R1.3341.00
159_L200_G1.3251.00
219_G223_N1.3181.00
69_C204_V1.3151.00
19_M23_T1.3141.00
212_F231_K1.3061.00
168_K172_E1.3021.00
233_Q237_I1.2941.00
82_K168_K1.2931.00
224_L229_V1.2791.00
78_W163_I1.2741.00
74_Y197_I1.2531.00
169_P172_E1.2491.00
50_L53_E1.2241.00
57_H60_N1.2081.00
64_I67_L1.2061.00
214_V218_M1.2051.00
174_A188_I1.2031.00
90_L93_F1.2011.00
233_Q239_D1.1991.00
166_L192_L1.1941.00
67_L71_V1.1841.00
58_S226_I1.1781.00
114_L142_A1.1781.00
146_G218_M1.1671.00
7_G11_L1.1481.00
180_S184_T1.1301.00
70_L197_I1.1241.00
194_M198_I1.1101.00
152_L156_V1.1101.00
115_S126_W1.1011.00
137_M140_P1.0941.00
68_A144_I1.0931.00
215_S225_L1.0801.00
143_V215_S1.0791.00
30_D37_V1.0711.00
232_V236_N1.0651.00
90_L94_L1.0621.00
81_A163_I1.0591.00
20_I248_I1.0441.00
64_I68_A1.0361.00
260_V264_A1.0351.00
211_L214_V1.0351.00
47_L52_F1.0351.00
192_L195_P1.0341.00
27_T30_D1.0321.00
144_I148_V1.0291.00
263_R266_R1.0281.00
65_A147_L1.0261.00
224_L228_N1.0251.00
158_P200_G1.0251.00
141_S145_I1.0231.00
159_L196_G1.0201.00
19_M22_G1.0171.00
225_L228_N1.0171.00
32_S35_K1.0141.00
78_W185_F1.0131.00
55_L226_I1.0121.00
75_P79_F1.0091.00
34_V37_V1.0061.00
179_A184_T1.0011.00
171_L181_K0.9951.00
65_A69_C0.9941.00
88_R164_E0.9901.00
184_T187_R0.9801.00
229_V246_T0.9781.00
59_L63_L0.9721.00
45_R48_D0.9631.00
211_L215_S0.9551.00
254_M258_L0.9431.00
77_A81_A0.9351.00
65_A144_I0.9351.00
234_F243_G0.9331.00
43_Y248_I0.9331.00
147_L208_A0.9271.00
32_S36_M0.9241.00
42_N241_P0.9181.00
60_N141_S0.9121.00
216_D228_N0.9121.00
67_L201_C0.9111.00
63_L67_L0.9091.00
79_F85_H0.9051.00
78_W181_K0.9041.00
26_L30_D0.8971.00
86_K89_P0.8861.00
110_L218_M0.8781.00
162_S193_T0.8761.00
159_L193_T0.8761.00
103_S150_I0.8741.00
193_T196_G0.8741.00
96_I157_M0.8731.00
73_G156_V0.8701.00
185_F190_I0.8671.00
107_I213_Y0.8591.00
194_M197_I0.8511.00
186_I190_I0.8501.00
24_S28_R0.8501.00
69_C73_G0.8491.00
55_L59_L0.8481.00
229_V232_V0.8451.00
60_N63_L0.8421.00
231_K235_L0.8401.00
179_A187_R0.8391.00
110_L146_G0.8371.00
51_Y246_T0.8341.00
89_P92_L0.8341.00
155_M159_L0.8341.00
111_K115_S0.8331.00
61_M140_P0.8321.00
260_V263_R0.8311.00
46_L249_T0.8241.00
138_F221_A0.8181.00
184_T189_I0.8171.00
80_L92_L0.8151.00
233_Q242_F0.8151.00
23_T26_L0.8151.00
61_M147_L0.8131.00
77_A156_V0.8121.00
146_G214_V0.8101.00
68_A71_V0.8071.00
129_V141_S0.8041.00
204_V208_A0.7981.00
141_S144_I0.7881.00
256_L260_V0.7881.00
233_Q246_T0.7861.00
77_A163_I0.7861.00
56_L59_L0.7821.00
64_I148_V0.7731.00
23_T244_A0.7711.00
106_R149_Y0.7571.00
73_G159_L0.7511.00
69_C151_L0.7501.00
42_N45_R0.7481.00
181_K185_F0.7471.00
25_F38_F0.7461.00
80_L160_Y0.7431.00
185_F189_I0.7431.00
250_L254_M0.7421.00
44_T47_L0.7391.00
10_V14_F0.7391.00
45_R239_D0.7381.00
30_D36_M0.7361.00
48_D52_F0.7311.00
80_L91_L0.7261.00
69_C155_M0.7241.00
219_G224_L0.7221.00
261_Y264_A0.7221.00
242_F246_T0.7211.00
170_L188_I0.7141.00
62_A66_T0.7121.00
70_L159_L0.7101.00
168_K175_R0.7091.00
58_S227_G0.7081.00
166_L193_T0.7071.00
96_I99_F0.7061.00
27_T37_V0.7051.00
54_V57_H0.7041.00
54_V226_I0.6991.00
103_S213_Y0.6991.00
151_L204_V0.6931.00
15_L18_L0.6911.00
66_T205_M0.6911.00
143_V219_G0.6911.00
244_A247_S0.6911.00
26_L35_K0.6901.00
230_I246_T0.6861.00
197_I200_G0.6861.00
226_I250_L0.6841.00
23_T28_R0.6831.00
173_A176_D0.6831.00
72_L148_V0.6821.00
137_M141_S0.6811.00
244_A248_I0.6771.00
107_I217_L0.6761.00
76_F152_L0.6741.00
20_I244_A0.6721.00
52_F55_L0.6701.00
173_A177_L0.6701.00
92_L157_M0.6691.00
27_T36_M0.6681.00
142_A146_G0.6631.00
159_L163_I0.6611.00
228_N232_V0.6601.00
211_L225_L0.6571.00
196_G200_G0.6571.00
79_F83_L0.6571.00
40_L43_Y0.6551.00
25_F39_T0.6551.00
194_M264_A0.6551.00
227_G250_L0.6521.00
151_L207_P0.6481.00
38_F41_D0.6441.00
85_H91_L0.6431.00
76_F95_L0.6431.00
69_C208_A0.6421.00
10_V13_V0.6411.00
189_I192_L0.6401.00
61_M143_V0.6401.00
143_V147_L0.6391.00
126_W129_V0.6381.00
66_T70_L0.6371.00
23_T27_T0.6351.00
20_I247_S0.6351.00
255_G259_L0.6351.00
142_A218_M0.6231.00
61_M144_I0.6231.00
241_P244_A0.6211.00
4_T7_G0.6181.00
87_V91_L0.6181.00
197_I201_C0.6101.00
112_I115_S0.6091.00
9_L13_V0.6081.00
49_P53_E0.6051.00
86_K90_L0.6041.00
115_S137_M0.6041.00
240_W244_A0.6011.00
104_L217_L0.5981.00
158_P196_G0.5931.00
69_C148_V0.5931.00
111_K137_M0.5911.00
247_S250_L0.5901.00
16_P24_S0.5871.00
215_S228_N0.5851.00
37_V40_L0.5821.00
78_W189_I0.5801.00
16_P20_I0.5801.00
228_N231_K0.5791.00
58_S209_M0.5781.00
99_F153_P0.5771.00
77_A160_Y0.5741.00
213_Y216_D0.5741.00
62_A208_A0.5731.00
46_L246_T0.5721.00
57_H223_N0.5711.00
37_V41_D0.5711.00
46_L242_F0.5681.00
24_S248_I0.5681.00
184_T188_I0.5671.00
26_L37_V0.5651.00
5_I9_L0.5641.00
137_M142_A0.5641.00
33_F36_M0.5631.00
186_I189_I0.5631.00
95_L153_P0.5601.00
253_V256_L0.5591.00
72_L152_L0.5591.00
16_P21_I0.5581.00
238_R242_F0.5571.00
61_M65_A0.5531.00
139_T142_A0.5531.00
50_L229_V0.5521.00
206_L210_G0.5521.00
89_P93_F0.5511.00
14_F18_L0.5491.00
83_L181_K0.5491.00
39_T43_Y0.5471.00
99_F154_F0.5441.00
70_L74_Y0.5431.00
26_L36_M0.5421.00
11_L15_L0.5421.00
215_S219_G0.5411.00
198_I201_C0.5411.00
189_I193_T0.5401.00
28_R35_K0.5391.00
16_P23_T0.5391.00
258_L262_W0.5381.00
21_I25_F0.5371.00
240_W243_G0.5371.00
230_I247_S0.5361.00
180_S186_I0.5361.00
66_T208_A0.5351.00
103_S107_I0.5351.00
213_Y217_L0.5341.00
201_C204_V0.5341.00
110_L113_F0.5321.00
147_L211_L0.5321.00
24_S245_A0.5301.00
50_L224_L0.5271.00
242_F245_A0.5251.00
98_P153_P0.5241.00
43_Y47_L0.5221.00
62_A209_M0.5201.00
101_T105_I0.5191.00
200_G204_V0.5181.00
147_L215_S0.5161.00
94_L98_P0.5151.00
49_P52_F0.5151.00
208_A211_L0.5131.00
34_V38_F0.5121.00
172_E175_R0.5121.00
84_P89_P0.5121.00
71_V74_Y0.5121.00
43_Y245_A0.5101.00
207_P210_G0.5091.00
84_P168_K0.5081.00
259_L262_W0.5081.00
107_I111_K0.5061.00
92_L164_E0.5061.00
57_H225_L0.5051.00
78_W82_K0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2onkC 2 0.8873 100 0.278 Contact Map
4tquM 1 0.9309 100 0.307 Contact Map
3d31C 2 0.8836 100 0.319 Contact Map
3rlfG 1 0.9564 100 0.354 Contact Map
3rlfF 1 0.92 100 0.357 Contact Map
4tquN 1 0.8945 100 0.372 Contact Map
4ymuD 2 0.7818 100 0.465 Contact Map
3tuiA 2 0.76 99.9 0.49 Contact Map
2mfrA 1 0.1855 11.4 0.946 Contact Map
2l2tA 2 0.1527 10.1 0.947 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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