GREMLIN Database
PHOB - Phosphate regulon transcriptional regulatory protein PhoB
UniProt: P0AFJ5 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10728
Length: 229 (209)
Sequences: 73847 (53114)
Seq/√Len: 3673.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
15_R31_E3.6921.00
15_R33_E3.2581.00
19_C31_E3.1731.00
72_S75_R2.7791.00
8_V38_A2.7291.00
39_V67_H2.6151.00
36_D58_G2.6071.00
35_Y59_G2.4221.00
59_G67_H2.4211.00
34_D58_G2.4181.00
43_N71_E2.4141.00
30_V47_P2.3941.00
4_R30_V2.2001.00
4_R28_Q2.1101.00
21_V109_P2.0661.00
32_A41_Q2.0411.00
19_C23_E2.0291.00
91_R111_E1.9941.00
33_E37_S1.8871.00
65_I79_V1.8821.00
25_N113_V1.8561.00
55_M85_R1.8471.00
52_L64_F1.8351.00
193_R196_D1.8141.00
81_M94_G1.7921.00
158_T161_K1.7691.00
88_E108_S1.7531.00
34_D37_S1.7411.00
6_L32_A1.7011.00
69_K100_D1.6861.00
81_M99_A1.6821.00
30_V41_Q1.6411.00
67_H71_E1.6301.00
136_G149_G1.6121.00
60_S63_Q1.6011.00
59_G63_Q1.5961.00
41_Q45_P1.5911.00
164_H168_T1.5571.00
35_Y64_F1.5191.00
176_R196_D1.5171.00
30_V45_P1.5131.00
165_F182_H1.4961.00
67_H70_R1.4951.00
22_L116_I1.4901.00
41_Q44_E1.4851.00
42_L68_L1.4791.00
101_D115_R1.4741.00
198_H201_R1.4381.00
65_I81_M1.4351.00
85_R90_D1.4321.00
36_D59_G1.4151.00
3_R120_M1.4011.00
80_V119_V1.4011.00
63_Q66_K1.3951.00
50_I77_I1.3841.00
49_L116_I1.3631.00
199_I203_R1.3491.00
100_D122_R1.3311.00
39_V64_F1.3201.00
54_W81_M1.3111.00
174_Y182_H1.3101.00
9_E15_R1.3041.00
66_K97_T1.2911.00
54_W62_I1.2811.00
52_L65_I1.2691.00
10_D53_D1.2591.00
39_V59_G1.2521.00
12_A33_E1.2481.00
41_Q47_P1.2401.00
8_V56_L1.2361.00
40_N44_E1.2201.00
165_F169_H1.2181.00
133_E138_S1.2151.00
37_S40_N1.1911.00
140_D143_S1.1851.00
175_S178_Q1.1771.00
37_S41_Q1.1721.00
27_F117_K1.1611.00
87_E90_D1.1601.00
36_D40_N1.1581.00
4_R45_P1.1571.00
6_L38_A1.1411.00
100_D119_V1.1391.00
32_A38_A1.1361.00
6_L47_P1.1241.00
96_E117_K1.1211.00
113_V117_K1.1181.00
62_I93_R1.1161.00
159_E205_A1.1071.00
26_G117_K1.1061.00
66_K93_R1.1061.00
72_S77_I1.0881.00
145_R152_P1.0821.00
139_L167_M1.0781.00
25_N117_K1.0641.00
88_E92_V1.0621.00
38_A64_F1.0601.00
165_F174_Y1.0601.00
6_L41_Q1.0591.00
111_E114_A1.0541.00
197_V200_R1.0521.00
160_F164_H1.0381.00
138_S147_M1.0351.00
194_T198_H1.0341.00
161_K164_H1.0241.00
62_I94_G1.0241.00
5_I27_F1.0201.00
208_P217_T1.0141.00
89_E93_R1.0121.00
16_E31_E1.0091.00
6_L42_L1.0081.00
178_Q182_H1.0061.00
49_L120_M1.0031.00
39_V43_N0.9961.00
93_R97_T0.9941.00
114_A118_A0.9861.00
3_R27_F0.9851.00
62_I97_T0.9791.00
144_H221_T0.9691.00
141_P164_H0.9681.00
91_R104_T0.9671.00
42_L72_S0.9651.00
32_A37_S0.9651.00
38_A42_L0.9641.00
177_E192_D0.9621.00
50_I68_L0.9591.00
92_V96_E0.9511.00
8_V50_I0.9451.00
18_V82_L0.9381.00
13_P17_M0.9241.00
119_V122_R0.9191.00
8_V32_A0.9161.00
52_L81_M0.9151.00
65_I97_T0.9121.00
157_P161_K0.9091.00
12_A16_E0.9081.00
3_R124_S0.9081.00
209_G212_D0.9051.00
52_L79_V0.9051.00
63_Q67_H0.9041.00
19_C29_P0.8991.00
193_R197_V0.8981.00
88_E91_R0.8981.00
7_V18_V0.8931.00
86_G104_T0.8881.00
193_R200_R0.8881.00
174_Y178_Q0.8791.00
40_N43_N0.8791.00
54_W60_S0.8761.00
147_M152_P0.8741.00
65_I99_A0.8741.00
78_P100_D0.8691.00
56_L64_F0.8661.00
93_R96_E0.8621.00
7_V29_P0.8621.00
201_R204_K0.8541.00
50_I79_V0.8541.00
133_E136_G0.8511.00
139_L160_F0.8491.00
4_R47_P0.8461.00
165_F179_L0.8371.00
27_F113_V0.8351.00
46_W72_S0.8321.00
131_V138_S0.8321.00
12_A15_R0.8251.00
92_V110_K0.8221.00
51_L112_L0.8171.00
208_P213_R0.8161.00
20_F24_Q0.8141.00
16_E19_C0.8131.00
66_K70_R0.8101.00
54_W90_D0.8031.00
51_L82_L0.7941.00
18_V112_L0.7891.00
42_L47_P0.7811.00
64_F68_L0.7761.00
118_A121_R0.7741.00
80_V115_R0.7711.00
200_R204_K0.7711.00
139_L146_V0.7701.00
4_R48_D0.7691.00
55_M84_A0.7681.00
192_D196_D0.7671.00
54_W85_R0.7651.00
118_A123_I0.7601.00
59_G64_F0.7541.00
109_P113_V0.7521.00
177_E181_N0.7471.00
207_E213_R0.7421.00
95_L115_R0.7411.00
68_L75_R0.7411.00
90_D93_R0.7411.00
111_E115_R0.7361.00
89_E110_K0.7351.00
108_S111_E0.7291.00
107_F112_L0.7271.00
209_G213_R0.7251.00
217_T221_T0.7221.00
8_V64_F0.7211.00
42_L77_I0.7171.00
35_Y39_V0.7141.00
144_H161_K0.7091.00
80_V101_D0.7051.00
147_M150_E0.7021.00
7_V19_C0.7011.00
156_G205_A0.7011.00
39_V42_L0.6991.00
82_L107_F0.6961.00
101_D118_A0.6901.00
136_G140_D0.6901.00
43_N72_S0.6861.00
16_E20_F0.6861.00
202_L205_A0.6831.00
110_K114_A0.6801.00
7_V31_E0.6791.00
71_E75_R0.6781.00
72_S76_D0.6771.00
68_L79_V0.6771.00
35_Y58_G0.6761.00
42_L50_I0.6751.00
165_F168_T0.6741.00
168_T182_H0.6731.00
3_R48_D0.6721.00
120_M123_I0.6721.00
178_Q181_N0.6671.00
96_E114_A0.6671.00
148_A151_E0.6661.00
9_E56_L0.6651.00
197_V201_R0.6631.00
195_V199_I0.6631.00
43_N67_H0.6601.00
88_E110_K0.6591.00
94_G99_A0.6551.00
17_M21_V0.6541.00
78_P126_M0.6521.00
136_G148_A0.6521.00
76_D126_M0.6481.00
199_I202_L0.6461.00
15_R19_C0.6461.00
212_D215_V0.6421.00
162_L166_F0.6281.00
123_I127_A0.6221.00
91_R108_S0.6181.00
191_E194_T0.6171.00
78_P122_R0.6161.00
69_K77_I0.6141.00
210_G213_R0.6131.00
139_L164_H0.6121.00
194_T199_I0.6091.00
53_D105_K0.6081.00
65_I69_K0.6071.00
203_R208_P0.6051.00
51_L80_V0.6051.00
131_V140_D0.6051.00
137_L148_A0.6041.00
140_D145_R0.6011.00
166_F199_I0.6011.00
11_E84_A0.5991.00
39_V71_E0.5981.00
14_I107_F0.5961.00
207_E212_D0.5911.00
51_L116_I0.5901.00
62_I90_D0.5871.00
156_G159_E0.5871.00
54_W94_G0.5861.00
22_L27_F0.5851.00
161_K165_F0.5841.00
115_R119_V0.5841.00
212_D220_G0.5831.00
76_D125_P0.5821.00
21_V113_V0.5811.00
89_E92_V0.5801.00
83_T91_R0.5791.00
107_F111_E0.5791.00
180_L183_V0.5771.00
10_D83_T0.5761.00
144_H160_F0.5751.00
35_Y56_L0.5741.00
42_L64_F0.5721.00
114_A117_K0.5721.00
168_T174_Y0.5711.00
78_P120_M0.5711.00
141_P168_T0.5701.00
5_I22_L0.5701.00
176_R192_D0.5691.00
5_I49_L0.5641.00
11_E14_I0.5611.00
23_E29_P0.5571.00
69_K78_P0.5561.00
176_R180_L0.5561.00
117_K121_R0.5541.00
166_F169_H0.5501.00
190_V195_V0.5481.00
159_E162_L0.5431.00
23_E28_Q0.5421.00
148_A207_E0.5381.00
92_V95_L0.5351.00
131_V134_M0.5321.00
200_R203_R0.5301.00
52_L56_L0.5231.00
68_L77_I0.5211.00
213_R216_Q0.5211.00
134_M138_S0.5191.00
84_A106_P0.5191.00
116_I120_M0.5191.00
118_A122_R0.5131.00
148_A208_P0.5101.00
27_F120_M0.5091.00
207_E214_M0.5071.00
37_S58_G0.5061.00
83_T105_K0.5051.00
198_H202_L0.5041.00
212_D216_Q0.5031.00
10_D56_L0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1kgsA 1 0.9563 100 0.208 Contact Map
2gwrA 1 0.9694 100 0.211 Contact Map
1ys7A 2 0.9782 100 0.211 Contact Map
3r0jA 2 0.9607 100 0.214 Contact Map
2oqrA 2 0.9694 100 0.227 Contact Map
4b09A 2 0.9214 100 0.24 Contact Map
4kfcA 2 0.9694 100 0.242 Contact Map
1p2fA 1 0.9432 100 0.254 Contact Map
2hqrA 2 0.9476 100 0.255 Contact Map
3q9sA 1 0.9127 100 0.261 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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