PERM - ECOLI - GREMLIN Contacts

Home
GREMLIN
- Submit - Monomer
- Submit - Complex
Contacts
Metagenomics
- Contacts
- Structures
Structures
- List
Old Projects
- Complexes (2014)
- PFAM (2013)

PERM - Putative permease PerM

UniProt:	P0AFI9	Sequences: MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLVAIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRDMPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLLTIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVLEMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGTEFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPLATLIKAVIHAWPDGQIAQE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG14221
Length:	353 (325)
Sequences:	10986 (8594)
Seq/√Len:	476.7

ECOLI - PERM TQSA YDIK YHHT
BACSU - YRRI YUBA YUEF YTVI YDBI
HALSA - Q9HPX5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
230_G	284_V	5.182	1.00
228_W	243_A	4.914	1.00
58_R	62_I	3.506	1.00
315_S	335_A	3.143	1.00
58_R	61_S	2.872	1.00
50_Y	206_N	2.816	1.00
224_G	247_G	2.714	1.00
276_T	280_S	2.602	1.00
65_S	68_W	2.588	1.00
279_W	283_A	2.513	1.00
228_W	232_L	2.500	1.00
224_G	243_A	2.371	1.00
265_V	282_F	2.368	1.00
48_L	173_V	2.353	1.00
64_C	72_I	2.263	1.00
102_D	105_G	2.219	1.00
129_I	132_A	2.129	1.00
339_K	343_H	2.122	1.00
265_V	278_F	2.005	1.00
29_F	33_F	2.004	1.00
60_Q	66_R	1.995	1.00
231_F	236_L	1.928	1.00
280_S	283_A	1.901	1.00
53_E	57_V	1.888	1.00
45_A	173_V	1.877	1.00
200_Q	344_A	1.864	1.00
283_A	287_I	1.843	1.00
204_E	344_A	1.837	1.00
56_T	73_V	1.790	1.00
111_S	115_A	1.782	1.00
217_V	251_L	1.781	1.00
136_N	139_S	1.775	1.00
188_R	199_G	1.761	1.00
174_F	178_K	1.753	1.00
70_T	174_F	1.721	1.00
215_G	300_L	1.720	1.00
245_L	249_S	1.706	1.00
299_V	303_E	1.694	1.00
239_S	243_A	1.679	1.00
56_T	70_T	1.677	1.00
229_L	232_L	1.662	1.00
227_T	246_V	1.661	1.00
169_V	173_V	1.660	1.00
272_F	277_E	1.658	1.00
205_M	337_L	1.658	1.00
67_R	71_S	1.636	1.00
112_D	116_T	1.635	1.00
130_I	133_M	1.630	1.00
227_T	288_I	1.628	1.00
53_E	66_R	1.617	1.00
113_F	116_T	1.601	1.00
172_M	310_L	1.595	1.00
108_N	111_S	1.585	1.00
228_W	239_S	1.584	1.00
70_T	74_L	1.572	1.00
191_L	198_A	1.564	1.00
55_P	73_V	1.561	1.00
179_D	308_H	1.556	1.00
82_L	86_F	1.555	1.00
277_E	280_S	1.543	1.00
67_R	178_K	1.533	1.00
42_L	314_L	1.530	1.00
115_A	118_P	1.520	1.00
132_A	135_E	1.504	1.00
232_L	239_S	1.501	1.00
97_I	101_R	1.499	1.00
25_L	29_F	1.498	1.00
59_L	76_V	1.495	1.00
320_G	328_V	1.482	1.00
226_A	288_I	1.481	1.00
296_L	300_L	1.481	1.00
56_T	66_R	1.480	1.00
121_Y	124_L	1.464	1.00
172_M	176_L	1.457	1.00
105_G	108_N	1.455	1.00
120_R	123_A	1.454	1.00
223_V	250_V	1.450	1.00
237_N	271_Q	1.432	1.00
286_L	290_A	1.431	1.00
118_P	122_P	1.418	1.00
26_V	30_G	1.416	1.00
131_D	135_E	1.415	1.00
185_N	189_R	1.407	1.00
109_K	112_D	1.387	1.00
64_C	68_W	1.372	1.00
291_L	296_L	1.370	1.00
249_S	260_V	1.368	1.00
143_T	146_D	1.367	1.00
122_P	125_M	1.367	1.00
261_T	265_V	1.346	1.00
132_A	136_N	1.344	1.00
33_F	36_S	1.340	1.00
52_L	177_L	1.338	1.00
268_A	281_C	1.333	1.00
229_L	233_L	1.313	1.00
134_A	137_M	1.310	1.00
105_G	109_K	1.285	1.00
89_L	93_W	1.282	1.00
116_T	120_R	1.280	1.00
264_V	281_C	1.280	1.00
18_A	22_L	1.269	1.00
316_V	328_V	1.261	1.00
242_L	264_V	1.255	1.00
179_D	182_Q	1.250	1.00
282_F	286_L	1.247	1.00
135_E	138_R	1.238	1.00
230_G	234_F	1.238	1.00
276_T	279_W	1.232	1.00
113_F	117_L	1.229	1.00
71_S	75_V	1.224	1.00
192_P	195_R	1.213	1.00
120_R	124_L	1.210	1.00
223_V	288_I	1.209	1.00
230_G	280_S	1.209	1.00
128_G	131_D	1.206	1.00
208_Q	336_T	1.206	1.00
55_P	58_R	1.191	1.00
204_E	343_H	1.187	1.00
100_I	104_P	1.186	1.00
49_A	176_L	1.182	1.00
236_L	268_A	1.182	1.00
152_S	156_L	1.177	1.00
56_T	69_A	1.173	1.00
214_R	218_L	1.167	1.00
56_T	177_L	1.165	1.00
128_G	132_A	1.159	1.00
69_A	73_V	1.159	1.00
41_P	45_A	1.154	1.00
78_V	82_L	1.152	1.00
52_L	77_F	1.150	1.00
268_A	278_F	1.146	1.00
45_A	169_V	1.143	1.00
113_F	118_P	1.142	1.00
186_A	189_R	1.141	1.00
200_Q	204_E	1.139	1.00
201_V	341_V	1.137	1.00
275_G	278_F	1.135	1.00
22_L	26_V	1.130	1.00
19_I	23_V	1.129	1.00
315_S	319_F	1.127	1.00
234_F	284_V	1.123	1.00
243_A	247_G	1.121	1.00
67_R	174_F	1.118	1.00
64_C	69_A	1.115	1.00
30_G	34_F	1.115	1.00
66_R	181_E	1.114	1.00
53_E	180_K	1.112	1.00
278_F	282_F	1.109	1.00
219_E	292_D	1.106	1.00
138_R	142_L	1.099	1.00
311_V	335_A	1.098	1.00
119_R	122_P	1.096	1.00
46_I	176_L	1.093	1.00
109_K	113_F	1.091	1.00
269_L	278_F	1.090	1.00
319_F	323_W	1.083	1.00
30_G	33_F	1.080	1.00
289_Q	293_G	1.078	1.00
182_Q	185_N	1.068	1.00
275_G	279_W	1.065	1.00
44_V	169_V	1.064	1.00
68_W	71_S	1.061	1.00
326_W	330_F	1.060	1.00
48_L	169_V	1.055	1.00
104_P	108_N	1.053	1.00
139_S	143_T	1.052	1.00
228_W	240_L	1.051	1.00
287_I	291_L	1.050	1.00
217_V	221_I	1.046	1.00
112_D	115_A	1.042	1.00
129_I	133_M	1.042	1.00
127_A	131_D	1.040	1.00
220_M	250_V	1.035	1.00
280_S	284_V	1.034	1.00
121_Y	125_M	1.030	1.00
291_L	295_L	1.027	1.00
219_E	296_L	1.024	1.00
45_A	49_A	1.018	1.00
144_M	148_V	1.014	1.00
110_L	113_F	1.006	1.00
42_L	172_M	1.005	1.00
162_I	166_L	1.001	1.00
19_I	22_L	1.000	1.00
267_V	271_Q	0.997	1.00
180_K	184_L	0.994	1.00
293_G	297_V	0.993	1.00
231_F	242_L	0.991	1.00
172_M	314_L	0.984	1.00
59_L	73_V	0.983	1.00
234_F	277_E	0.983	1.00
221_I	247_G	0.982	1.00
216_K	297_V	0.981	1.00
137_M	140_R	0.978	1.00
224_G	228_W	0.977	1.00
230_G	288_I	0.972	1.00
315_S	331_A	0.970	1.00
218_L	222_V	0.967	1.00
93_W	96_G	0.966	1.00
319_F	335_A	0.960	1.00
155_S	159_L	0.952	1.00
138_R	141_M	0.948	1.00
24_I	28_G	0.948	1.00
338_I	342_I	0.947	1.00
284_V	288_I	0.947	1.00
168_L	172_M	0.945	1.00
74_L	173_V	0.943	1.00
295_L	299_V	0.942	1.00
94_Q	98_Y	0.940	1.00
184_L	188_R	0.939	1.00
219_E	223_V	0.934	1.00
114_A	118_P	0.930	1.00
223_V	257_A	0.928	1.00
234_F	281_C	0.925	1.00
311_V	339_K	0.924	1.00
52_L	73_V	0.924	1.00
98_Y	101_R	0.923	1.00
97_I	100_I	0.922	1.00
211_N	215_G	0.918	1.00
272_F	275_G	0.917	1.00
223_V	227_T	0.917	1.00
231_F	246_V	0.914	1.00
238_Y	241_L	0.912	1.00
57_V	60_Q	0.910	1.00
207_Q	211_N	0.909	1.00
86_F	90_P	0.906	1.00
102_D	106_M	0.905	1.00
21_L	25_L	0.905	1.00
245_L	260_V	0.903	1.00
225_I	229_L	0.901	1.00
182_Q	186_A	0.899	1.00
140_R	143_T	0.898	1.00
211_N	304_A	0.895	1.00
188_R	195_R	0.893	1.00
269_L	275_G	0.892	1.00
108_N	112_D	0.891	1.00
38_L	318_I	0.891	1.00
136_N	140_R	0.891	1.00
115_A	119_R	0.890	1.00
247_G	251_L	0.889	1.00
29_F	32_I	0.880	1.00
142_L	146_D	0.880	1.00
243_A	246_V	0.877	1.00
198_A	202_W	0.869	1.00
231_F	281_C	0.867	1.00
225_I	228_W	0.856	1.00
26_V	33_F	0.855	1.00
301_F	312_I	0.854	1.00
156_L	160_L	0.851	1.00
290_A	294_N	0.846	1.00
85_A	89_L	0.844	1.00
91_I	94_Q	0.843	1.00
28_G	31_I	0.843	1.00
103_M	107_L	0.838	1.00
80_I	84_M	0.833	1.00
219_E	297_V	0.828	1.00
232_L	243_A	0.827	1.00
133_M	136_N	0.827	1.00
197_L	200_Q	0.824	1.00
201_V	344_A	0.824	1.00
139_S	142_L	0.822	1.00
331_A	335_A	0.818	1.00
222_V	226_A	0.815	1.00
221_I	225_I	0.807	1.00
261_T	285_Y	0.803	1.00
20_A	24_I	0.802	1.00
141_M	144_M	0.797	1.00
196_G	200_Q	0.796	1.00
58_R	63_G	0.795	1.00
314_L	318_I	0.793	1.00
341_V	345_W	0.787	1.00
259_V	263_P	0.786	1.00
250_V	257_A	0.785	1.00
220_M	224_G	0.778	1.00
22_L	25_L	0.776	1.00
266_G	270_F	0.775	1.00
148_V	152_S	0.770	1.00
158_G	161_T	0.769	1.00
186_A	190_V	0.765	1.00
232_L	235_G	0.761	1.00
101_R	105_G	0.760	1.00
222_V	296_L	0.758	1.00
218_L	300_L	0.755	1.00
234_F	280_S	0.755	1.00
190_V	342_I	0.753	1.00
292_D	297_V	0.753	1.00
227_T	231_F	0.750	1.00
143_T	147_S	0.748	1.00
147_S	150_K	0.747	1.00
98_Y	102_D	0.745	1.00
246_V	260_V	0.745	1.00
42_L	176_L	0.745	1.00
242_L	267_V	0.745	1.00
59_L	69_A	0.745	1.00
220_M	251_L	0.744	1.00
199_G	203_K	0.737	1.00
56_T	60_Q	0.736	1.00
107_L	111_S	0.735	1.00
45_A	172_M	0.733	1.00
49_A	173_V	0.730	1.00
219_E	301_F	0.727	1.00
237_N	240_L	0.727	1.00
218_L	221_I	0.723	1.00
210_T	214_R	0.722	1.00
44_V	48_L	0.719	1.00
204_E	208_Q	0.719	1.00
119_R	123_A	0.718	1.00
57_V	181_E	0.715	1.00
219_E	250_V	0.713	1.00
153_L	157_V	0.712	1.00
26_V	29_F	0.712	1.00
96_G	100_I	0.710	1.00
24_I	31_I	0.709	1.00
161_T	165_Y	0.709	1.00
307_L	335_A	0.709	1.00
224_G	244_V	0.708	1.00
21_L	33_F	0.702	1.00
195_R	199_G	0.702	1.00
223_V	297_V	0.701	1.00
265_V	269_L	0.699	1.00
185_N	188_R	0.699	1.00
147_S	151_I	0.696	1.00
319_F	334_L	0.695	1.00
19_I	24_I	0.694	1.00
197_L	344_A	0.694	1.00
271_Q	277_E	0.693	1.00
246_V	285_Y	0.692	1.00
236_L	239_S	0.692	1.00
53_E	177_L	0.690	1.00
134_A	140_R	0.689	1.00
131_D	134_A	0.688	1.00
158_G	162_I	0.684	1.00
245_L	263_P	0.682	1.00
60_Q	69_A	0.680	1.00
208_Q	211_N	0.680	1.00
34_F	37_G	0.679	1.00
188_R	191_L	0.679	1.00
187_V	190_V	0.678	1.00
95_Q	98_Y	0.677	1.00
164_V	168_L	0.672	1.00
204_E	340_A	0.671	1.00
66_R	70_T	0.669	1.00
101_R	104_P	0.667	1.00
92_A	96_G	0.666	1.00
297_V	301_F	0.665	1.00
297_V	300_L	0.665	1.00
168_L	317_V	0.664	1.00
195_R	198_A	0.663	1.00
186_A	311_V	0.663	1.00
257_A	289_Q	0.662	1.00
149_V	152_S	0.662	1.00
148_V	151_I	0.661	1.00
130_I	134_A	0.658	1.00
103_M	106_M	0.656	1.00
227_T	243_A	0.656	1.00
162_I	165_Y	0.653	1.00
203_K	207_Q	0.653	1.00
23_V	27_A	0.653	1.00
130_I	136_N	0.651	1.00
114_A	120_R	0.645	1.00
27_A	31_I	0.645	1.00
161_T	294_N	0.644	1.00
205_M	340_A	0.644	1.00
82_L	85_A	0.642	1.00
129_I	134_A	0.641	1.00
309_P	313_I	0.639	1.00
246_V	250_V	0.638	1.00
59_L	72_I	0.638	1.00
185_N	199_G	0.637	1.00
268_A	272_F	0.636	1.00
146_D	150_K	0.635	1.00
52_L	55_P	0.634	1.00
80_I	83_L	0.634	1.00
117_L	120_R	0.632	1.00
216_K	220_M	0.628	1.00
201_V	205_M	0.627	1.00
250_V	297_V	0.627	1.00
149_V	153_L	0.626	1.00
133_M	137_M	0.626	1.00
287_I	290_A	0.625	0.99
134_A	138_R	0.625	0.99
140_R	144_M	0.623	0.99
137_M	141_M	0.621	0.99
59_L	64_C	0.620	0.99
60_Q	63_G	0.620	0.99
254_Y	309_P	0.619	0.99
81_L	85_A	0.616	0.99
302_S	312_I	0.615	0.99
114_A	117_L	0.615	0.99
151_I	155_S	0.615	0.99
227_T	284_V	0.615	0.99
305_V	332_I	0.612	0.99
21_L	24_I	0.611	0.99
83_L	87_V	0.610	0.99
260_V	264_V	0.610	0.99
312_I	332_I	0.608	0.99
145_G	148_V	0.607	0.99
110_L	114_A	0.607	0.99
335_A	339_K	0.604	0.99
57_V	61_S	0.603	0.99
166_L	170_P	0.602	0.99
41_P	302_S	0.601	0.99
211_N	214_R	0.598	0.99
323_W	327_G	0.598	0.99
204_E	207_Q	0.598	0.99
184_L	203_K	0.595	0.99
173_V	177_L	0.593	0.99
93_W	97_I	0.593	0.99
22_L	29_F	0.593	0.99
108_N	115_A	0.591	0.99
28_G	32_I	0.591	0.99
135_E	139_S	0.591	0.99
23_V	28_G	0.590	0.99
224_G	240_L	0.590	0.99
76_V	79_G	0.590	0.99
52_L	173_V	0.590	0.99
307_L	312_I	0.590	0.99
74_L	174_F	0.586	0.99
316_V	332_I	0.586	0.99
41_P	165_Y	0.583	0.99
296_L	299_V	0.582	0.99
325_F	328_V	0.581	0.99
90_P	333_P	0.581	0.99
49_A	177_L	0.580	0.99
51_L	213_I	0.578	0.99
303_E	306_N	0.575	0.99
117_L	121_Y	0.575	0.99
247_G	254_Y	0.571	0.99
246_V	257_A	0.570	0.99
79_G	82_L	0.568	0.99
216_K	250_V	0.565	0.99
219_E	300_L	0.564	0.99
49_A	53_E	0.563	0.99
145_G	149_V	0.563	0.99
116_T	119_R	0.560	0.99
140_R	145_G	0.556	0.99
75_V	79_G	0.554	0.99
302_S	306_N	0.554	0.99
226_A	230_G	0.552	0.99
299_V	302_S	0.551	0.99
70_T	178_K	0.550	0.99
188_R	192_P	0.550	0.99
272_F	278_F	0.549	0.99
102_D	237_N	0.546	0.99
209_I	212_Y	0.545	0.99
96_G	104_P	0.541	0.99
127_A	130_I	0.541	0.99
220_M	223_V	0.541	0.99
205_M	208_Q	0.539	0.99
65_S	69_A	0.538	0.98
40_A	43_L	0.537	0.98
91_I	95_Q	0.532	0.98
92_A	95_Q	0.531	0.98
223_V	226_A	0.531	0.98
227_T	247_G	0.530	0.98
313_I	317_V	0.530	0.98
216_K	219_E	0.530	0.98
87_V	91_I	0.528	0.98
246_V	264_V	0.526	0.98
104_P	107_L	0.523	0.98
205_M	209_I	0.521	0.98
197_L	201_V	0.521	0.98
171_L	294_N	0.520	0.98
219_E	257_A	0.520	0.98
160_L	164_V	0.518	0.98
25_L	28_G	0.516	0.98
183_M	186_A	0.516	0.98
144_M	147_S	0.515	0.98
215_G	336_T	0.515	0.98
260_V	332_I	0.514	0.98
88_V	92_A	0.514	0.98
176_L	310_L	0.513	0.98
311_V	315_S	0.513	0.98
18_A	21_L	0.513	0.98
118_P	121_Y	0.512	0.98
250_V	292_D	0.512	0.98
224_G	261_T	0.512	0.98
261_T	286_L	0.507	0.98
201_V	204_E	0.507	0.98
208_Q	337_L	0.507	0.98
175_F	179_D	0.504	0.98
181_E	185_N	0.503	0.98
258_F	261_T	0.503	0.98
31_I	35_F	0.502	0.98
259_V	289_Q	0.502	0.98
21_L	30_G	0.502	0.98
297_V	332_I	0.502	0.98
184_L	202_W	0.500	0.98
72_I	76_V	0.500	0.98

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4lz6A	1	0.8952	24.5	0.953	Contact Map
2kv5A	1	0.0907	15.3	0.957	Contact Map
4gycB	2	0.1728	11.4	0.959	Contact Map
3w4tA	1	0.8612	11.1	0.96	Contact Map
4b19A	1	0.085	11	0.96	Contact Map
4humA	1	0.8725	7.4	0.963	Contact Map
4wgvA	2	0.238	5.7	0.965	Contact Map
3mktA	1	0.8839	5.3	0.965	Contact Map
3pjsK	4	0.3654	5.1	0.965	Contact Map
4bgnA	3	0.2861	5.1	0.965	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0111 seconds.