GREMLIN Database
PERM - Putative permease PerM
UniProt: P0AFI9 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG14221
Length: 353 (325)
Sequences: 10986 (8594)
Seq/√Len: 476.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
230_G284_V5.1821.00
228_W243_A4.9141.00
58_R62_I3.5061.00
315_S335_A3.1431.00
58_R61_S2.8721.00
50_Y206_N2.8161.00
224_G247_G2.7141.00
276_T280_S2.6021.00
65_S68_W2.5881.00
279_W283_A2.5131.00
228_W232_L2.5001.00
224_G243_A2.3711.00
265_V282_F2.3681.00
48_L173_V2.3531.00
64_C72_I2.2631.00
102_D105_G2.2191.00
129_I132_A2.1291.00
339_K343_H2.1221.00
265_V278_F2.0051.00
29_F33_F2.0041.00
60_Q66_R1.9951.00
231_F236_L1.9281.00
280_S283_A1.9011.00
53_E57_V1.8881.00
45_A173_V1.8771.00
200_Q344_A1.8641.00
283_A287_I1.8431.00
204_E344_A1.8371.00
56_T73_V1.7901.00
111_S115_A1.7821.00
217_V251_L1.7811.00
136_N139_S1.7751.00
188_R199_G1.7611.00
174_F178_K1.7531.00
70_T174_F1.7211.00
215_G300_L1.7201.00
245_L249_S1.7061.00
299_V303_E1.6941.00
239_S243_A1.6791.00
56_T70_T1.6771.00
229_L232_L1.6621.00
227_T246_V1.6611.00
169_V173_V1.6601.00
272_F277_E1.6581.00
205_M337_L1.6581.00
67_R71_S1.6361.00
112_D116_T1.6351.00
130_I133_M1.6301.00
227_T288_I1.6281.00
53_E66_R1.6171.00
113_F116_T1.6011.00
172_M310_L1.5951.00
108_N111_S1.5851.00
228_W239_S1.5841.00
70_T74_L1.5721.00
191_L198_A1.5641.00
55_P73_V1.5611.00
179_D308_H1.5561.00
82_L86_F1.5551.00
277_E280_S1.5431.00
67_R178_K1.5331.00
42_L314_L1.5301.00
115_A118_P1.5201.00
132_A135_E1.5041.00
232_L239_S1.5011.00
97_I101_R1.4991.00
25_L29_F1.4981.00
59_L76_V1.4951.00
320_G328_V1.4821.00
226_A288_I1.4811.00
296_L300_L1.4811.00
56_T66_R1.4801.00
121_Y124_L1.4641.00
172_M176_L1.4571.00
105_G108_N1.4551.00
120_R123_A1.4541.00
223_V250_V1.4501.00
237_N271_Q1.4321.00
286_L290_A1.4311.00
118_P122_P1.4181.00
26_V30_G1.4161.00
131_D135_E1.4151.00
185_N189_R1.4071.00
109_K112_D1.3871.00
64_C68_W1.3721.00
291_L296_L1.3701.00
249_S260_V1.3681.00
143_T146_D1.3671.00
122_P125_M1.3671.00
261_T265_V1.3461.00
132_A136_N1.3441.00
33_F36_S1.3401.00
52_L177_L1.3381.00
268_A281_C1.3331.00
229_L233_L1.3131.00
134_A137_M1.3101.00
105_G109_K1.2851.00
89_L93_W1.2821.00
116_T120_R1.2801.00
264_V281_C1.2801.00
18_A22_L1.2691.00
316_V328_V1.2611.00
242_L264_V1.2551.00
179_D182_Q1.2501.00
282_F286_L1.2471.00
135_E138_R1.2381.00
230_G234_F1.2381.00
276_T279_W1.2321.00
113_F117_L1.2291.00
71_S75_V1.2241.00
192_P195_R1.2131.00
120_R124_L1.2101.00
223_V288_I1.2091.00
230_G280_S1.2091.00
128_G131_D1.2061.00
208_Q336_T1.2061.00
55_P58_R1.1911.00
204_E343_H1.1871.00
100_I104_P1.1861.00
49_A176_L1.1821.00
236_L268_A1.1821.00
152_S156_L1.1771.00
56_T69_A1.1731.00
214_R218_L1.1671.00
56_T177_L1.1651.00
128_G132_A1.1591.00
69_A73_V1.1591.00
41_P45_A1.1541.00
78_V82_L1.1521.00
52_L77_F1.1501.00
268_A278_F1.1461.00
45_A169_V1.1431.00
113_F118_P1.1421.00
186_A189_R1.1411.00
200_Q204_E1.1391.00
201_V341_V1.1371.00
275_G278_F1.1351.00
22_L26_V1.1301.00
19_I23_V1.1291.00
315_S319_F1.1271.00
234_F284_V1.1231.00
243_A247_G1.1211.00
67_R174_F1.1181.00
64_C69_A1.1151.00
30_G34_F1.1151.00
66_R181_E1.1141.00
53_E180_K1.1121.00
278_F282_F1.1091.00
219_E292_D1.1061.00
138_R142_L1.0991.00
311_V335_A1.0981.00
119_R122_P1.0961.00
46_I176_L1.0931.00
109_K113_F1.0911.00
269_L278_F1.0901.00
319_F323_W1.0831.00
30_G33_F1.0801.00
289_Q293_G1.0781.00
182_Q185_N1.0681.00
275_G279_W1.0651.00
44_V169_V1.0641.00
68_W71_S1.0611.00
326_W330_F1.0601.00
48_L169_V1.0551.00
104_P108_N1.0531.00
139_S143_T1.0521.00
228_W240_L1.0511.00
287_I291_L1.0501.00
217_V221_I1.0461.00
112_D115_A1.0421.00
129_I133_M1.0421.00
127_A131_D1.0401.00
220_M250_V1.0351.00
280_S284_V1.0341.00
121_Y125_M1.0301.00
291_L295_L1.0271.00
219_E296_L1.0241.00
45_A49_A1.0181.00
144_M148_V1.0141.00
110_L113_F1.0061.00
42_L172_M1.0051.00
162_I166_L1.0011.00
19_I22_L1.0001.00
267_V271_Q0.9971.00
180_K184_L0.9941.00
293_G297_V0.9931.00
231_F242_L0.9911.00
172_M314_L0.9841.00
59_L73_V0.9831.00
234_F277_E0.9831.00
221_I247_G0.9821.00
216_K297_V0.9811.00
137_M140_R0.9781.00
224_G228_W0.9771.00
230_G288_I0.9721.00
315_S331_A0.9701.00
218_L222_V0.9671.00
93_W96_G0.9661.00
319_F335_A0.9601.00
155_S159_L0.9521.00
138_R141_M0.9481.00
24_I28_G0.9481.00
338_I342_I0.9471.00
284_V288_I0.9471.00
168_L172_M0.9451.00
74_L173_V0.9431.00
295_L299_V0.9421.00
94_Q98_Y0.9401.00
184_L188_R0.9391.00
219_E223_V0.9341.00
114_A118_P0.9301.00
223_V257_A0.9281.00
234_F281_C0.9251.00
311_V339_K0.9241.00
52_L73_V0.9241.00
98_Y101_R0.9231.00
97_I100_I0.9221.00
211_N215_G0.9181.00
272_F275_G0.9171.00
223_V227_T0.9171.00
231_F246_V0.9141.00
238_Y241_L0.9121.00
57_V60_Q0.9101.00
207_Q211_N0.9091.00
86_F90_P0.9061.00
102_D106_M0.9051.00
21_L25_L0.9051.00
245_L260_V0.9031.00
225_I229_L0.9011.00
182_Q186_A0.8991.00
140_R143_T0.8981.00
211_N304_A0.8951.00
188_R195_R0.8931.00
269_L275_G0.8921.00
108_N112_D0.8911.00
38_L318_I0.8911.00
136_N140_R0.8911.00
115_A119_R0.8901.00
247_G251_L0.8891.00
29_F32_I0.8801.00
142_L146_D0.8801.00
243_A246_V0.8771.00
198_A202_W0.8691.00
231_F281_C0.8671.00
225_I228_W0.8561.00
26_V33_F0.8551.00
301_F312_I0.8541.00
156_L160_L0.8511.00
290_A294_N0.8461.00
85_A89_L0.8441.00
91_I94_Q0.8431.00
28_G31_I0.8431.00
103_M107_L0.8381.00
80_I84_M0.8331.00
219_E297_V0.8281.00
232_L243_A0.8271.00
133_M136_N0.8271.00
197_L200_Q0.8241.00
201_V344_A0.8241.00
139_S142_L0.8221.00
331_A335_A0.8181.00
222_V226_A0.8151.00
221_I225_I0.8071.00
261_T285_Y0.8031.00
20_A24_I0.8021.00
141_M144_M0.7971.00
196_G200_Q0.7961.00
58_R63_G0.7951.00
314_L318_I0.7931.00
341_V345_W0.7871.00
259_V263_P0.7861.00
250_V257_A0.7851.00
220_M224_G0.7781.00
22_L25_L0.7761.00
266_G270_F0.7751.00
148_V152_S0.7701.00
158_G161_T0.7691.00
186_A190_V0.7651.00
232_L235_G0.7611.00
101_R105_G0.7601.00
222_V296_L0.7581.00
218_L300_L0.7551.00
234_F280_S0.7551.00
190_V342_I0.7531.00
292_D297_V0.7531.00
227_T231_F0.7501.00
143_T147_S0.7481.00
147_S150_K0.7471.00
98_Y102_D0.7451.00
246_V260_V0.7451.00
42_L176_L0.7451.00
242_L267_V0.7451.00
59_L69_A0.7451.00
220_M251_L0.7441.00
199_G203_K0.7371.00
56_T60_Q0.7361.00
107_L111_S0.7351.00
45_A172_M0.7331.00
49_A173_V0.7301.00
219_E301_F0.7271.00
237_N240_L0.7271.00
218_L221_I0.7231.00
210_T214_R0.7221.00
44_V48_L0.7191.00
204_E208_Q0.7191.00
119_R123_A0.7181.00
57_V181_E0.7151.00
219_E250_V0.7131.00
153_L157_V0.7121.00
26_V29_F0.7121.00
96_G100_I0.7101.00
24_I31_I0.7091.00
161_T165_Y0.7091.00
307_L335_A0.7091.00
224_G244_V0.7081.00
21_L33_F0.7021.00
195_R199_G0.7021.00
223_V297_V0.7011.00
265_V269_L0.6991.00
185_N188_R0.6991.00
147_S151_I0.6961.00
319_F334_L0.6951.00
19_I24_I0.6941.00
197_L344_A0.6941.00
271_Q277_E0.6931.00
246_V285_Y0.6921.00
236_L239_S0.6921.00
53_E177_L0.6901.00
134_A140_R0.6891.00
131_D134_A0.6881.00
158_G162_I0.6841.00
245_L263_P0.6821.00
60_Q69_A0.6801.00
208_Q211_N0.6801.00
34_F37_G0.6791.00
188_R191_L0.6791.00
187_V190_V0.6781.00
95_Q98_Y0.6771.00
164_V168_L0.6721.00
204_E340_A0.6711.00
66_R70_T0.6691.00
101_R104_P0.6671.00
92_A96_G0.6661.00
297_V301_F0.6651.00
297_V300_L0.6651.00
168_L317_V0.6641.00
195_R198_A0.6631.00
186_A311_V0.6631.00
257_A289_Q0.6621.00
149_V152_S0.6621.00
148_V151_I0.6611.00
130_I134_A0.6581.00
103_M106_M0.6561.00
227_T243_A0.6561.00
162_I165_Y0.6531.00
203_K207_Q0.6531.00
23_V27_A0.6531.00
130_I136_N0.6511.00
114_A120_R0.6451.00
27_A31_I0.6451.00
161_T294_N0.6441.00
205_M340_A0.6441.00
82_L85_A0.6421.00
129_I134_A0.6411.00
309_P313_I0.6391.00
246_V250_V0.6381.00
59_L72_I0.6381.00
185_N199_G0.6371.00
268_A272_F0.6361.00
146_D150_K0.6351.00
52_L55_P0.6341.00
80_I83_L0.6341.00
117_L120_R0.6321.00
216_K220_M0.6281.00
201_V205_M0.6271.00
250_V297_V0.6271.00
149_V153_L0.6261.00
133_M137_M0.6261.00
287_I290_A0.6250.99
134_A138_R0.6250.99
140_R144_M0.6230.99
137_M141_M0.6210.99
59_L64_C0.6200.99
60_Q63_G0.6200.99
254_Y309_P0.6190.99
81_L85_A0.6160.99
302_S312_I0.6150.99
114_A117_L0.6150.99
151_I155_S0.6150.99
227_T284_V0.6150.99
305_V332_I0.6120.99
21_L24_I0.6110.99
83_L87_V0.6100.99
260_V264_V0.6100.99
312_I332_I0.6080.99
145_G148_V0.6070.99
110_L114_A0.6070.99
335_A339_K0.6040.99
57_V61_S0.6030.99
166_L170_P0.6020.99
41_P302_S0.6010.99
211_N214_R0.5980.99
323_W327_G0.5980.99
204_E207_Q0.5980.99
184_L203_K0.5950.99
173_V177_L0.5930.99
93_W97_I0.5930.99
22_L29_F0.5930.99
108_N115_A0.5910.99
28_G32_I0.5910.99
135_E139_S0.5910.99
23_V28_G0.5900.99
224_G240_L0.5900.99
76_V79_G0.5900.99
52_L173_V0.5900.99
307_L312_I0.5900.99
74_L174_F0.5860.99
316_V332_I0.5860.99
41_P165_Y0.5830.99
296_L299_V0.5820.99
325_F328_V0.5810.99
90_P333_P0.5810.99
49_A177_L0.5800.99
51_L213_I0.5780.99
303_E306_N0.5750.99
117_L121_Y0.5750.99
247_G254_Y0.5710.99
246_V257_A0.5700.99
79_G82_L0.5680.99
216_K250_V0.5650.99
219_E300_L0.5640.99
49_A53_E0.5630.99
145_G149_V0.5630.99
116_T119_R0.5600.99
140_R145_G0.5560.99
75_V79_G0.5540.99
302_S306_N0.5540.99
226_A230_G0.5520.99
299_V302_S0.5510.99
70_T178_K0.5500.99
188_R192_P0.5500.99
272_F278_F0.5490.99
102_D237_N0.5460.99
209_I212_Y0.5450.99
96_G104_P0.5410.99
127_A130_I0.5410.99
220_M223_V0.5410.99
205_M208_Q0.5390.99
65_S69_A0.5380.98
40_A43_L0.5370.98
91_I95_Q0.5320.98
92_A95_Q0.5310.98
223_V226_A0.5310.98
227_T247_G0.5300.98
313_I317_V0.5300.98
216_K219_E0.5300.98
87_V91_I0.5280.98
246_V264_V0.5260.98
104_P107_L0.5230.98
205_M209_I0.5210.98
197_L201_V0.5210.98
171_L294_N0.5200.98
219_E257_A0.5200.98
160_L164_V0.5180.98
25_L28_G0.5160.98
183_M186_A0.5160.98
144_M147_S0.5150.98
215_G336_T0.5150.98
260_V332_I0.5140.98
88_V92_A0.5140.98
176_L310_L0.5130.98
311_V315_S0.5130.98
18_A21_L0.5130.98
118_P121_Y0.5120.98
250_V292_D0.5120.98
224_G261_T0.5120.98
261_T286_L0.5070.98
201_V204_E0.5070.98
208_Q337_L0.5070.98
175_F179_D0.5040.98
181_E185_N0.5030.98
258_F261_T0.5030.98
31_I35_F0.5020.98
259_V289_Q0.5020.98
21_L30_G0.5020.98
297_V332_I0.5020.98
184_L202_W0.5000.98
72_I76_V0.5000.98
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4lz6A 1 0.8952 24.5 0.953 Contact Map
2kv5A 1 0.0907 15.3 0.957 Contact Map
4gycB 2 0.1728 11.4 0.959 Contact Map
3w4tA 1 0.8612 11.1 0.96 Contact Map
4b19A 1 0.085 11 0.96 Contact Map
4humA 1 0.8725 7.4 0.963 Contact Map
4wgvA 2 0.238 5.7 0.965 Contact Map
3mktA 1 0.8839 5.3 0.965 Contact Map
3pjsK 4 0.3654 5.1 0.965 Contact Map
4bgnA 3 0.2861 5.1 0.965 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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