NAGC - ECOLI - GREMLIN Contacts

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NAGC - N-acetylglucosamine repressor

UniProt:	P0AF20	Sequences: MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQLAPASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITLFDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDPDSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILVRVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQRVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFNPQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKRAMLNGILLQHLLEN	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG10636
Length:	406 (367)
Sequences:	6626 (4918)
Seq/√Len:	256.7

ECOLI - NAGC MLC YPHH MAK ALSK NAGK NANK
BACSU - XYLR GLK SCRK
HALSA - Q9HMA7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
104_D	108_K	3.954	1.00
112_E	381_R	3.385	1.00
177_G	180_E	3.295	1.00
193_G	236_I	3.270	1.00
63_K	82_E	3.129	1.00
217_L	341_A	3.054	1.00
149_A	390_V	3.001	1.00
257_C	264_C	2.800	1.00
103_F	387_F	2.751	1.00
89_I	133_I	2.725	1.00
259_C	264_C	2.681	1.00
159_D	162_S	2.610	1.00
150_I	186_F	2.570	1.00
239_N	334_N	2.558	1.00
27_I	33_I	2.539	1.00
216_I	329_A	2.514	1.00
63_K	80_V	2.498	1.00
248_I	328_I	2.484	1.00
277_R	317_Y	2.440	1.00
316_E	354_A	2.348	1.00
130_L	185_R	2.344	1.00
179_V	183_E	2.330	1.00
319_G	323_G	2.325	1.00
101_T	383_A	2.275	1.00
320_R	358_C	2.269	1.00
127_H	185_R	2.226	1.00
90_G	383_A	2.189	1.00
265_L	325_T	2.134	1.00
113_E	135_Q	2.108	1.00
337_K	374_V	2.100	1.00
27_I	62_I	2.090	1.00
212_C	337_K	2.087	1.00
100_I	136_F	2.068	1.00
113_E	139_S	2.054	1.00
100_I	132_A	2.004	1.00
23_V	38_I	1.975	1.00
209_S	215_S	1.963	1.00
57_I	64_E	1.936	1.00
230_S	235_F	1.924	1.00
326_I	338_I	1.905	1.00
200_A	229_I	1.870	1.00
180_E	184_E	1.843	1.00
247_H	257_C	1.829	1.00
152_V	182_L	1.815	1.00
20_S	59_R	1.812	1.00
87_H	110_L	1.808	1.00
319_G	355_I	1.800	1.00
134_A	185_R	1.788	1.00
256_R	262_F	1.780	1.00
65_V	80_V	1.779	1.00
20_S	55_Q	1.772	1.00
257_C	260_G	1.761	1.00
53_T	62_I	1.759	1.00
150_I	188_V	1.747	1.00
153_I	198_S	1.734	1.00
218_V	326_I	1.715	1.00
247_H	259_C	1.706	1.00
213_E	336_Q	1.689	1.00
193_G	198_S	1.667	1.00
214_D	336_Q	1.658	1.00
200_A	227_G	1.657	1.00
323_G	358_C	1.641	1.00
181_A	184_E	1.640	1.00
64_E	77_I	1.635	1.00
194_H	244_E	1.625	1.00
199_L	217_L	1.625	1.00
193_G	197_R	1.623	1.00
89_I	147_L	1.617	1.00
147_L	188_V	1.607	1.00
352_L	356_E	1.604	1.00
215_S	229_I	1.600	1.00
359_I	373_V	1.598	1.00
247_H	264_C	1.597	1.00
206_F	388_A	1.576	1.00
111_A	136_F	1.568	1.00
249_Q	328_I	1.566	1.00
356_E	360_N	1.562	1.00
324_K	328_I	1.558	1.00
111_A	139_S	1.552	1.00
238_R	336_Q	1.546	1.00
38_I	49_V	1.543	1.00
302_K	306_K	1.542	1.00
296_T	299_T	1.535	1.00
30_Y	41_Q	1.531	1.00
213_E	231_N	1.529	1.00
91_V	129_L	1.508	1.00
53_T	79_I	1.503	1.00
224_T	269_A	1.500	1.00
84_R	104_D	1.499	1.00
278_V	314_V	1.492	1.00
337_K	372_P	1.491	1.00
37_Q	40_E	1.463	1.00
304_A	315_I	1.441	1.00
97_D	114_H	1.436	1.00
319_G	354_A	1.433	1.00
305_N	350_V	1.430	1.00
349_K	353_P	1.424	1.00
357_S	361_T	1.422	1.00
86_F	148_I	1.421	1.00
143_K	146_E	1.395	1.00
354_A	357_S	1.385	1.00
26_L	33_I	1.374	1.00
200_A	215_S	1.373	1.00
299_T	302_K	1.355	1.00
57_I	79_I	1.350	1.00
303_A	308_D	1.336	1.00
102_L	136_F	1.336	1.00
260_G	264_C	1.328	1.00
91_V	133_I	1.326	1.00
19_N	52_I	1.320	1.00
130_L	186_F	1.315	1.00
134_A	144_L	1.304	1.00
99_T	114_H	1.293	1.00
203_E	207_G	1.292	1.00
101_T	109_V	1.290	1.00
255_E	267_T	1.288	1.00
26_L	41_Q	1.287	1.00
176_W	181_A	1.279	1.00
255_E	276_Q	1.277	1.00
35_R	79_I	1.273	1.00
202_A	378_L	1.263	1.00
88_A	391_K	1.249	1.00
91_V	100_I	1.248	1.00
157_L	168_M	1.247	1.00
237_G	243_G	1.243	1.00
205_Y	392_R	1.227	1.00
255_E	268_I	1.227	1.00
20_S	52_I	1.227	1.00
194_H	223_G	1.227	1.00
134_A	186_F	1.223	1.00
113_E	132_A	1.218	1.00
90_G	386_A	1.217	1.00
198_S	386_A	1.214	1.00
212_C	336_Q	1.212	1.00
358_C	361_T	1.194	1.00
115_Y	135_Q	1.191	1.00
274_I	318_V	1.182	1.00
329_A	332_L	1.181	1.00
348_D	352_L	1.177	1.00
277_R	280_N	1.174	1.00
358_C	362_Q	1.161	1.00
25_R	29_Q	1.160	1.00
237_G	242_V	1.131	1.00
182_L	190_C	1.129	1.00
23_V	49_V	1.127	1.00
298_K	302_K	1.126	1.00
316_E	357_S	1.126	1.00
22_A	42_S	1.126	1.00
90_G	151_S	1.122	1.00
37_Q	41_Q	1.121	1.00
115_Y	132_A	1.120	1.00
33_I	41_Q	1.119	1.00
35_R	64_E	1.116	1.00
64_E	79_I	1.113	1.00
223_G	244_E	1.105	1.00
278_V	311_A	1.105	1.00
216_I	226_A	1.103	1.00
32_P	80_V	1.102	1.00
66_D	78_S	1.099	1.00
135_Q	139_S	1.096	1.00
24_Y	81_T	1.093	1.00
132_A	135_Q	1.088	1.00
215_S	339_V	1.086	1.00
330_I	338_I	1.083	1.00
123_Q	181_A	1.079	1.00
231_N	235_F	1.074	1.00
87_H	142_R	1.071	1.00
126_E	181_A	1.065	1.00
164_K	177_G	1.062	1.00
187_K	190_C	1.060	1.00
83_T	106_S	1.057	1.00
353_P	356_E	1.057	1.00
255_E	277_R	1.051	1.00
352_L	375_R	1.049	1.00
125_L	173_V	1.044	1.00
247_H	260_G	1.042	1.00
330_I	359_I	1.041	1.00
122_Q	173_V	1.030	1.00
277_R	314_V	1.030	1.00
330_I	371_L	1.026	1.00
197_R	234_I	1.026	1.00
26_L	42_S	1.025	1.00
87_H	146_E	1.019	1.00
299_T	303_A	1.014	1.00
127_H	131_N	1.005	1.00
281_L	314_V	1.003	1.00
46_P	50_T	1.002	1.00
191_F	393_A	0.997	1.00
102_L	140_Y	0.996	1.00
214_D	231_N	0.995	1.00
204_H	232_G	0.990	1.00
257_C	263_G	0.990	1.00
204_H	229_I	0.990	1.00
103_F	109_V	0.989	1.00
252_P	324_K	0.988	1.00
199_L	341_A	0.983	1.00
60_G	82_E	0.974	1.00
216_I	228_I	0.969	1.00
235_F	333_F	0.966	1.00
177_G	181_A	0.961	1.00
142_R	146_E	0.953	1.00
228_I	235_F	0.950	1.00
89_I	137_I	0.950	1.00
379_D	382_S	0.945	1.00
203_E	378_L	0.944	1.00
134_A	138_D	0.941	1.00
312_S	316_E	0.939	1.00
277_R	281_L	0.938	1.00
36_I	76_A	0.938	1.00
109_V	112_E	0.935	1.00
354_A	358_C	0.932	1.00
115_Y	128_A	0.932	1.00
204_H	215_S	0.931	1.00
340_I	345_T	0.927	1.00
323_G	327_A	0.921	1.00
121_T	124_T	0.920	1.00
151_S	198_S	0.919	1.00
173_V	176_W	0.918	1.00
326_I	359_I	0.917	1.00
183_E	187_K	0.917	1.00
89_I	150_I	0.914	1.00
88_A	390_V	0.912	1.00
302_K	305_N	0.911	1.00
195_D	225_G	0.910	1.00
151_S	390_V	0.910	1.00
122_Q	176_W	0.910	1.00
89_I	102_L	0.905	1.00
27_I	38_I	0.902	1.00
137_I	147_L	0.902	1.00
353_P	357_S	0.900	1.00
128_A	132_A	0.900	1.00
264_C	267_T	0.900	1.00
181_A	185_R	0.895	1.00
251_E	268_I	0.891	1.00
228_I	333_F	0.890	1.00
220_V	322_L	0.890	1.00
91_V	150_I	0.880	1.00
32_P	78_S	0.879	1.00
99_T	112_E	0.878	1.00
218_V	245_I	0.877	1.00
183_E	190_C	0.874	1.00
207_G	210_Q	0.873	1.00
196_I	219_R	0.868	1.00
61_L	85_N	0.866	1.00
168_M	171_I	0.865	1.00
54_R	58_E	0.863	1.00
339_V	376_S	0.862	1.00
98_A	117_L	0.862	1.00
111_A	140_Y	0.861	1.00
28_D	81_T	0.860	1.00
63_K	83_T	0.859	1.00
88_A	103_F	0.859	1.00
277_R	318_V	0.858	0.99
281_L	313_E	0.858	0.99
214_D	238_R	0.857	0.99
223_G	266_E	0.856	0.99
131_N	135_Q	0.855	0.99
320_R	354_A	0.854	0.99
304_A	350_V	0.854	0.99
323_G	359_I	0.852	0.99
44_L	49_V	0.850	0.99
348_D	375_R	0.850	0.99
164_K	175_N	0.848	0.99
276_Q	280_N	0.845	0.99
93_L	98_A	0.844	0.99
304_A	312_S	0.844	0.99
324_K	362_Q	0.843	0.99
124_T	127_H	0.843	0.99
257_C	267_T	0.843	0.99
225_G	244_E	0.839	0.99
19_N	44_L	0.839	0.99
249_Q	252_P	0.836	0.99
251_E	254_G	0.831	0.99
349_K	375_R	0.830	0.99
196_I	227_G	0.825	0.99
137_I	144_L	0.824	0.99
261_N	362_Q	0.823	0.99
237_G	334_N	0.821	0.99
57_I	62_I	0.818	0.99
310_L	313_E	0.818	0.99
198_S	389_L	0.817	0.99
339_V	374_V	0.816	0.99
115_Y	131_N	0.816	0.99
27_I	79_I	0.814	0.99
130_L	182_L	0.814	0.99
68_Q	76_A	0.813	0.99
212_C	215_S	0.805	0.99
346_E	380_H	0.803	0.99
213_E	232_G	0.802	0.99
191_F	390_V	0.800	0.99
278_V	300_I	0.798	0.99
317_Y	321_H	0.796	0.99
80_V	106_S	0.796	0.99
46_P	51_K	0.792	0.99
130_L	134_A	0.791	0.99
301_C	304_A	0.791	0.99
88_A	148_I	0.791	0.99
197_R	227_G	0.790	0.99
214_D	230_S	0.784	0.99
154_L	158_V	0.784	0.99
220_V	344_I	0.782	0.99
210_Q	377_E	0.780	0.99
203_E	376_S	0.778	0.99
348_D	353_P	0.773	0.99
223_G	246_G	0.772	0.99
378_L	382_S	0.769	0.99
194_H	225_G	0.765	0.99
319_G	351_L	0.764	0.99
133_I	150_I	0.756	0.99
36_I	40_E	0.754	0.99
199_L	382_S	0.752	0.99
322_L	355_I	0.749	0.99
259_C	263_G	0.747	0.99
304_A	307_G	0.746	0.99
101_T	112_E	0.745	0.99
30_Y	33_I	0.743	0.99
301_C	305_N	0.740	0.99
154_L	165_I	0.736	0.98
207_G	211_D	0.736	0.98
136_F	140_Y	0.734	0.98
274_I	300_I	0.733	0.98
242_V	334_N	0.732	0.98
254_G	268_I	0.731	0.98
305_N	349_K	0.731	0.98
376_S	382_S	0.731	0.98
163_G	177_G	0.730	0.98
304_A	311_A	0.729	0.98
279_L	293_D	0.727	0.98
248_I	325_T	0.726	0.98
260_G	263_G	0.726	0.98
261_N	264_C	0.725	0.98
274_I	314_V	0.725	0.98
82_E	85_N	0.725	0.98
334_N	371_L	0.725	0.98
22_A	26_L	0.722	0.98
320_R	324_K	0.720	0.98
26_L	38_I	0.720	0.98
195_D	219_R	0.713	0.98
98_A	115_Y	0.708	0.98
126_E	178_L	0.707	0.98
320_R	357_S	0.706	0.98
251_E	255_E	0.704	0.98
249_Q	261_N	0.700	0.98
281_L	285_G	0.697	0.98
128_A	131_N	0.695	0.98
113_E	136_F	0.694	0.98
123_Q	127_H	0.693	0.98
351_L	354_A	0.691	0.98
316_E	358_C	0.691	0.98
308_D	311_A	0.690	0.98
295_C	299_T	0.690	0.98
249_Q	362_Q	0.689	0.98
45_A	48_S	0.688	0.98
378_L	384_I	0.688	0.98
275_E	300_I	0.687	0.98
184_E	187_K	0.686	0.98
257_C	261_N	0.684	0.98
157_L	241_N	0.683	0.98
313_E	317_Y	0.682	0.97
155_P	170_H	0.680	0.97
193_G	389_L	0.680	0.97
118_P	128_A	0.680	0.97
103_F	384_I	0.679	0.97
92_R	194_H	0.679	0.97
104_D	110_L	0.679	0.97
34_S	76_A	0.676	0.97
205_Y	388_A	0.675	0.97
25_R	28_D	0.673	0.97
194_H	197_R	0.672	0.97
221_H	343_E	0.672	0.97
84_R	110_L	0.671	0.97
68_Q	71_T	0.670	0.97
259_C	267_T	0.670	0.97
83_T	104_D	0.669	0.97
254_G	264_C	0.669	0.97
179_V	192_V	0.667	0.97
102_L	111_A	0.666	0.97
102_L	147_L	0.666	0.97
19_N	22_A	0.664	0.97
350_V	354_A	0.664	0.97
300_I	315_I	0.662	0.97
65_V	78_S	0.660	0.97
94_G	343_E	0.660	0.97
275_E	297_I	0.658	0.97
208_A	377_E	0.656	0.97
83_T	108_K	0.656	0.97
297_I	300_I	0.655	0.97
156_G	266_E	0.655	0.97
214_D	235_F	0.653	0.97
247_H	263_G	0.652	0.97
245_I	332_L	0.651	0.97
68_Q	72_G	0.649	0.97
103_F	107_S	0.648	0.97
35_R	46_P	0.646	0.97
360_N	370_N	0.645	0.96
109_V	384_I	0.644	0.96
352_L	355_I	0.643	0.96
137_I	140_Y	0.642	0.96
245_I	265_L	0.639	0.96
303_A	306_K	0.639	0.96
53_T	57_I	0.639	0.96
163_G	179_V	0.638	0.96
92_R	153_I	0.636	0.96
254_G	262_F	0.635	0.96
265_L	318_V	0.632	0.96
197_R	244_E	0.631	0.96
23_V	52_I	0.621	0.96
336_Q	372_P	0.621	0.96
313_E	316_E	0.620	0.96
61_L	105_L	0.618	0.96
151_S	191_F	0.617	0.95
90_G	387_F	0.616	0.95
238_R	335_P	0.615	0.95
247_H	254_G	0.614	0.95
212_C	372_P	0.613	0.95
228_I	243_G	0.611	0.95
323_G	362_Q	0.609	0.95
238_R	334_N	0.609	0.95
42_S	49_V	0.608	0.95
215_S	337_K	0.608	0.95
131_N	134_A	0.607	0.95
224_T	318_V	0.606	0.95
255_E	280_N	0.605	0.95
321_H	325_T	0.603	0.95
203_E	377_E	0.602	0.95
26_L	30_Y	0.600	0.95
316_E	319_G	0.598	0.95
250_V	325_T	0.597	0.95
281_L	310_L	0.596	0.95
301_C	315_I	0.594	0.94
327_A	331_N	0.594	0.94
349_K	352_L	0.593	0.94
134_A	137_I	0.592	0.94
271_N	275_E	0.590	0.94
268_I	277_R	0.588	0.94
35_R	223_G	0.586	0.94
132_A	136_F	0.585	0.94
148_I	390_V	0.583	0.94
141_Q	144_L	0.582	0.94
298_K	346_E	0.580	0.94
92_R	343_E	0.579	0.94
98_A	125_L	0.578	0.94
218_V	226_A	0.578	0.94
85_N	146_E	0.578	0.94
208_A	211_D	0.576	0.94
254_G	257_C	0.574	0.93
94_G	222_R	0.574	0.93
92_R	99_T	0.573	0.93
99_T	383_A	0.572	0.93
217_L	339_V	0.572	0.93
172_Q	175_N	0.571	0.93
82_E	86_F	0.571	0.93
303_A	311_A	0.570	0.93
206_F	384_I	0.570	0.93
49_V	53_T	0.570	0.93
55_Q	59_R	0.569	0.93
87_H	102_L	0.569	0.93
257_C	262_F	0.568	0.93
374_V	377_E	0.568	0.93
297_I	301_C	0.567	0.93
208_A	339_V	0.567	0.93
191_F	389_L	0.566	0.93
100_I	115_Y	0.566	0.93
251_E	324_K	0.566	0.93
155_P	168_M	0.565	0.93
182_L	186_F	0.564	0.93
216_I	338_I	0.563	0.93
83_T	86_F	0.562	0.93
327_A	362_Q	0.561	0.93
55_Q	58_E	0.560	0.93
149_A	191_F	0.558	0.92
24_Y	61_L	0.557	0.92
334_N	363_A	0.557	0.92
363_A	368_R	0.553	0.92
201_L	389_L	0.552	0.92
180_E	183_E	0.552	0.92
297_I	343_E	0.551	0.92
300_I	304_A	0.551	0.92
117_L	125_L	0.549	0.92
102_L	142_R	0.549	0.92
268_I	321_H	0.548	0.92
253_L	320_R	0.548	0.92
98_A	129_L	0.548	0.92
94_G	97_D	0.547	0.92
348_D	354_A	0.546	0.92
225_G	266_E	0.544	0.91
159_D	166_H	0.544	0.91
226_A	245_I	0.544	0.91
200_A	217_L	0.543	0.91
149_A	387_F	0.541	0.91
51_K	54_R	0.541	0.91
292_L	295_C	0.540	0.91
371_L	375_R	0.539	0.91
327_A	358_C	0.539	0.91
341_A	376_S	0.538	0.91
199_L	385_G	0.538	0.91
86_F	146_E	0.537	0.91
95_R	222_R	0.537	0.91
267_T	276_Q	0.536	0.91
51_K	55_Q	0.535	0.91
126_E	176_W	0.535	0.91
35_R	50_T	0.534	0.91
112_E	383_A	0.534	0.91
348_D	351_L	0.533	0.91
349_K	356_E	0.533	0.91
57_I	61_L	0.533	0.91
26_L	29_Q	0.532	0.91
250_V	321_H	0.532	0.91
309_S	313_E	0.531	0.90
131_N	138_D	0.531	0.90
204_H	213_E	0.530	0.90
248_I	265_L	0.529	0.90
312_S	350_V	0.528	0.90
319_G	358_C	0.528	0.90
273_A	276_Q	0.527	0.90
84_R	146_E	0.527	0.90
129_L	182_L	0.527	0.90
90_G	153_I	0.525	0.90
67_Q	78_S	0.524	0.90
314_V	318_V	0.522	0.90
249_Q	254_G	0.521	0.90
237_G	240_G	0.518	0.89
275_E	279_L	0.516	0.89
204_H	214_D	0.516	0.89
93_L	152_V	0.514	0.89
271_N	343_E	0.514	0.89
280_N	285_G	0.514	0.89
197_R	201_L	0.513	0.89
250_V	268_I	0.513	0.89
326_I	355_I	0.513	0.89
92_R	219_R	0.513	0.89
272_A	276_Q	0.512	0.89
141_Q	146_E	0.509	0.89
357_S	360_N	0.507	0.88
74_R	314_V	0.507	0.88
144_L	188_V	0.507	0.88
326_I	329_A	0.506	0.88
88_A	149_A	0.505	0.88
54_R	57_I	0.504	0.88
208_A	374_V	0.503	0.88
249_Q	324_K	0.503	0.88
199_L	219_R	0.503	0.88
207_G	374_V	0.503	0.88
263_G	267_T	0.503	0.88
279_L	285_G	0.502	0.88
177_G	183_E	0.502	0.88
92_R	97_D	0.502	0.88
230_S	335_P	0.501	0.88
195_D	223_G	0.500	0.88
275_E	295_C	0.500	0.88

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1z05A	2	0.9704	100	0.169	Contact Map
1z6rA	3	0.9384	100	0.176	Contact Map
2hoeA	2	0.867	100	0.256	Contact Map
4ijaA	2	0.9089	100	0.289	Contact Map
3o8mA	1	0.9113	100	0.394	Contact Map
2yhwA	2	0.7414	100	0.405	Contact Map
1bdgA	1	0.867	100	0.409	Contact Map
2ap1A	2	0.7217	100	0.416	Contact Map
4db3A	2	0.7365	100	0.418	Contact Map
3f9mA	1	0.8719	100	0.418	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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