MOTB - ECOLI - GREMLIN Contacts

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MOTB - Motility protein B

UniProt:	P0AF06	Sequences: MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVMWLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPNIEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPMFRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRANASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHENAESQNEPVSALEKPEVAPQVSVPTMPSAEPR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG10602
Length:	308 (254)
Sequences:	2761 (2209)
Seq/√Len:	138.6

ECOLI - MOTB YIAD MBHA
BACSU - YTXE MOTB

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
162_R	169_E	5.482	1.00
212_E	223_R	3.536	1.00
197_D	210_N	3.453	1.00
177_R	224_E	3.185	1.00
30_Y	34_M	2.945	1.00
208_Y	216_D	2.928	1.00
119_R	123_D	2.840	1.00
241_M	245_M	2.836	1.00
40_F	44_M	2.741	1.00
28_I	31_A	2.729	1.00
192_L	222_R	2.602	1.00
176_L	224_E	2.600	1.00
209_S	212_E	2.528	1.00
248_S	256_V	2.499	1.00
51_S	54_E	2.402	1.00
29_A	33_F	2.401	1.00
151_Q	261_S	2.310	1.00
50_S	55_L	2.302	1.00
117_K	121_D	2.273	1.00
250_R	254_D	2.262	1.00
249_D	252_P	2.248	1.00
29_A	38_M	2.227	1.00
163_T	199_F	2.194	1.00
199_F	254_D	2.190	1.00
40_F	43_V	2.163	1.00
195_H	245_M	2.159	1.00
168_V	224_E	2.147	1.00
214_S	240_G	2.117	1.00
203_S	208_Y	2.113	1.00
232_S	244_T	2.013	1.00
166_A	216_D	1.959	1.00
30_Y	35_T	1.912	1.00
215_A	240_G	1.857	1.00
222_R	238_V	1.851	1.00
154_D	158_R	1.805	1.00
38_M	42_L	1.743	1.00
25_S	29_A	1.726	1.00
215_A	219_N	1.704	1.00
166_A	207_G	1.693	1.00
24_G	27_K	1.678	1.00
219_N	222_R	1.671	1.00
52_P	56_I	1.658	1.00
183_L	230_L	1.645	1.00
198_D	254_D	1.569	1.00
89_D	92_Q	1.561	1.00
14_R	19_H	1.559	1.00
24_G	29_A	1.547	1.00
208_Y	212_E	1.543	1.00
248_S	254_D	1.530	1.00
72_G	76_I	1.497	1.00
34_M	40_F	1.491	1.00
72_G	77_S	1.478	1.00
73_G	77_S	1.478	1.00
201_Y	207_G	1.469	1.00
22_A	25_S	1.461	1.00
72_G	75_R	1.432	1.00
170_P	173_R	1.414	1.00
207_G	216_D	1.400	1.00
176_L	221_S	1.399	1.00
168_V	172_M	1.381	1.00
106_L	109_R	1.356	1.00
116_R	120_G	1.347	1.00
106_L	110_M	1.346	1.00
188_N	234_K	1.330	1.00
180_A	225_L	1.328	1.00
201_Y	204_G	1.305	1.00
22_A	29_A	1.304	1.00
226_M	232_S	1.287	1.00
24_G	43_V	1.285	1.00
72_G	78_N	1.280	1.00
76_I	80_E	1.278	1.00
193_S	263_L	1.257	1.00
198_D	250_R	1.255	1.00
163_T	197_D	1.251	1.00
191_S	239_V	1.232	1.00
92_Q	95_G	1.232	1.00
193_S	239_V	1.227	1.00
223_R	227_V	1.215	1.00
232_S	243_A	1.213	1.00
198_D	252_P	1.211	1.00
179_I	183_L	1.189	1.00
73_G	76_I	1.163	0.99
115_L	118_L	1.160	0.99
183_L	264_V	1.148	0.99
110_M	114_R	1.141	0.99
17_K	20_G	1.138	0.99
248_S	251_G	1.130	0.99
30_Y	44_M	1.127	0.99
164_G	210_N	1.123	0.99
210_N	258_R	1.121	0.99
111_E	115_L	1.118	0.99
29_A	40_F	1.118	0.99
193_S	241_M	1.117	0.99
224_E	227_V	1.114	0.99
50_S	54_E	1.112	0.99
201_Y	209_S	1.110	0.99
112_Q	116_R	1.108	0.99
29_A	46_L	1.104	0.99
177_R	228_G	1.104	0.99
20_G	23_H	1.103	0.99
49_I	52_P	1.101	0.99
28_I	33_F	1.101	0.99
200_P	210_N	1.098	0.99
177_R	181_P	1.092	0.99
21_A	24_G	1.092	0.99
91_T	96_E	1.085	0.99
116_R	119_R	1.070	0.99
86_G	89_D	1.058	0.99
209_S	223_R	1.050	0.99
29_A	36_A	1.044	0.99
75_R	78_N	1.044	0.99
216_D	219_N	1.042	0.99
224_E	228_G	1.040	0.99
173_R	224_E	1.040	0.99
197_D	258_R	1.038	0.99
149_R	261_S	1.037	0.99
164_G	197_D	1.037	0.99
72_G	81_S	1.033	0.99
23_H	28_I	1.032	0.99
25_S	38_M	1.029	0.99
27_K	36_A	1.028	0.99
201_Y	208_Y	1.024	0.99
113_S	116_R	1.021	0.99
20_G	24_G	1.017	0.99
33_F	38_M	1.014	0.99
13_R	16_A	1.012	0.98
33_F	39_A	1.010	0.98
39_A	45_W	1.006	0.98
28_I	39_A	0.994	0.98
42_L	46_L	0.991	0.98
13_R	18_S	0.987	0.98
118_L	123_D	0.973	0.98
208_Y	223_R	0.971	0.98
72_G	83_I	0.969	0.98
39_A	43_V	0.964	0.98
93_S	96_E	0.956	0.98
22_A	27_K	0.956	0.98
203_S	209_S	0.955	0.98
120_G	123_D	0.954	0.98
146_E	193_S	0.953	0.98
49_I	53_K	0.951	0.98
160_M	193_S	0.946	0.98
62_F	65_P	0.944	0.98
109_R	112_Q	0.943	0.98
142_D	151_Q	0.940	0.98
142_D	149_R	0.938	0.98
174_D	177_R	0.938	0.98
93_S	97_V	0.934	0.97
90_Y	95_G	0.931	0.97
24_G	46_L	0.919	0.97
72_G	80_E	0.911	0.97
195_H	242_A	0.907	0.97
84_P	87_G	0.902	0.97
144_V	147_G	0.896	0.97
211_W	238_V	0.891	0.97
69_A	73_G	0.890	0.97
146_E	149_R	0.885	0.96
68_T	72_G	0.885	0.96
147_G	265_L	0.885	0.96
100_Q	103_I	0.877	0.96
221_S	260_I	0.872	0.96
91_T	94_Q	0.871	0.96
36_A	42_L	0.868	0.96
120_G	124_Q	0.854	0.96
74_D	80_E	0.854	0.96
141_I	148_L	0.854	0.96
26_W	39_A	0.854	0.96
86_G	93_S	0.853	0.96
29_A	39_A	0.851	0.96
37_M	46_L	0.851	0.96
163_T	210_N	0.847	0.95
180_A	230_L	0.846	0.95
25_S	34_M	0.843	0.95
58_I	62_F	0.843	0.95
176_L	225_L	0.842	0.95
77_S	80_E	0.842	0.95
96_E	99_K	0.840	0.95
147_G	188_N	0.838	0.95
76_I	79_S	0.832	0.95
57_Q	60_E	0.828	0.95
46_L	61_Y	0.825	0.95
52_P	55_L	0.825	0.95
149_R	263_L	0.824	0.95
211_W	242_A	0.822	0.95
102_N	105_E	0.822	0.95
24_G	28_I	0.821	0.95
140_K	153_I	0.820	0.95
195_H	246_R	0.817	0.95
25_S	43_V	0.810	0.94
195_H	260_I	0.809	0.94
24_G	40_F	0.809	0.94
121_D	185_G	0.805	0.94
171_Y	174_D	0.804	0.94
215_A	238_V	0.799	0.94
60_E	64_T	0.794	0.94
87_G	95_G	0.793	0.94
118_L	125_L	0.793	0.94
203_S	206_K	0.792	0.94
212_E	219_N	0.791	0.94
59_A	62_F	0.791	0.94
42_L	45_W	0.785	0.93
32_D	41_F	0.781	0.93
43_V	46_L	0.781	0.93
90_Y	94_Q	0.779	0.93
168_V	221_S	0.772	0.93
56_I	60_E	0.771	0.93
36_A	46_L	0.765	0.92
78_N	81_S	0.764	0.92
71_T	74_D	0.764	0.92
191_S	237_R	0.763	0.92
72_G	82_P	0.762	0.92
102_N	107_K	0.757	0.92
15_K	18_S	0.757	0.92
73_G	79_S	0.757	0.92
125_L	128_S	0.755	0.92
29_A	43_V	0.753	0.92
77_S	82_P	0.751	0.92
30_Y	39_A	0.750	0.92
90_Y	93_S	0.749	0.92
84_P	95_G	0.747	0.91
152_I	176_L	0.739	0.91
220_A	223_R	0.736	0.91
203_S	207_G	0.733	0.91
248_S	252_P	0.731	0.91
29_A	55_L	0.727	0.90
95_G	99_K	0.724	0.90
29_A	42_L	0.722	0.90
94_Q	98_N	0.720	0.90
103_I	106_L	0.720	0.90
91_T	95_G	0.720	0.90
86_G	91_T	0.719	0.90
152_I	160_M	0.712	0.89
92_Q	96_E	0.710	0.89
231_D	234_K	0.709	0.89
230_L	236_L	0.708	0.89
184_N	229_G	0.706	0.89
16_A	19_H	0.704	0.89
162_R	165_S	0.704	0.89
198_D	253_D	0.703	0.89
77_S	81_S	0.701	0.89
163_T	201_Y	0.701	0.89
195_H	265_L	0.701	0.89
24_G	45_W	0.701	0.89
165_S	206_K	0.700	0.89
53_K	56_I	0.699	0.89
16_A	20_G	0.699	0.89
68_T	71_T	0.698	0.88
100_Q	110_M	0.697	0.88
29_A	34_M	0.696	0.88
152_I	193_S	0.696	0.88
124_Q	130_P	0.696	0.88
73_G	78_N	0.696	0.88
14_R	17_K	0.694	0.88
25_S	33_F	0.689	0.88
100_Q	104_E	0.683	0.87
27_K	46_L	0.679	0.87
21_A	25_S	0.678	0.87
75_R	80_E	0.677	0.87
28_I	43_V	0.674	0.87
84_P	90_Y	0.671	0.86
154_D	160_M	0.671	0.86
172_M	193_S	0.671	0.86
74_D	79_S	0.664	0.86
193_S	261_S	0.664	0.86
124_Q	128_S	0.664	0.86
164_G	200_P	0.662	0.86
86_G	90_Y	0.662	0.86
24_G	30_Y	0.658	0.85
117_K	120_G	0.655	0.85
87_G	94_Q	0.654	0.85
80_E	84_P	0.650	0.85
197_D	254_D	0.650	0.85
89_D	96_E	0.649	0.85
84_P	89_D	0.646	0.84
151_Q	188_N	0.645	0.84
85_G	89_D	0.640	0.84
103_I	114_R	0.640	0.84
69_A	80_E	0.635	0.83
219_N	238_V	0.635	0.83
115_L	148_L	0.635	0.83
19_H	24_G	0.633	0.83
88_D	91_T	0.632	0.83
146_E	263_L	0.632	0.83
109_R	113_S	0.631	0.83
29_A	61_Y	0.628	0.83
200_P	209_S	0.628	0.83
219_N	223_R	0.628	0.83
226_M	236_L	0.627	0.82
53_K	57_Q	0.625	0.82
101_P	104_E	0.623	0.82
103_I	107_K	0.623	0.82
129_D	132_L	0.622	0.82
139_L	226_M	0.621	0.82
85_G	88_D	0.620	0.82
115_L	121_D	0.620	0.82
192_L	219_N	0.618	0.82
67_A	70_V	0.617	0.81
198_D	243_A	0.615	0.81
107_K	110_M	0.612	0.81
162_R	168_V	0.612	0.81
119_R	143_L	0.612	0.81
78_N	84_P	0.610	0.81
210_N	214_S	0.610	0.81
59_A	63_R	0.610	0.81
41_F	45_W	0.609	0.81
139_L	175_I	0.609	0.81
75_R	79_S	0.609	0.81
108_K	112_Q	0.606	0.80
184_N	228_G	0.601	0.80
29_A	45_W	0.600	0.80
85_G	90_Y	0.599	0.80
123_D	127_E	0.597	0.79
24_G	36_A	0.596	0.79
108_K	111_E	0.596	0.79
27_K	35_T	0.592	0.79
50_S	58_I	0.588	0.78
40_F	46_L	0.587	0.78
267_K	270_E	0.584	0.78
68_T	73_G	0.584	0.78
162_R	167_D	0.583	0.78
178_A	181_P	0.581	0.77
36_A	45_W	0.580	0.77
67_A	71_T	0.579	0.77
225_L	230_L	0.577	0.77
196_T	258_R	0.573	0.77
22_A	40_F	0.573	0.77
86_G	96_E	0.571	0.76
22_A	45_W	0.570	0.76
55_L	58_I	0.569	0.76
158_R	255_A	0.567	0.76
195_H	261_S	0.566	0.76
102_N	106_L	0.566	0.76
83_I	87_G	0.565	0.76
34_M	48_S	0.565	0.76
197_D	201_Y	0.565	0.76
87_G	90_Y	0.564	0.75
141_I	261_S	0.562	0.75
216_D	220_A	0.561	0.75
126_I	129_D	0.560	0.75
125_L	133_R	0.556	0.74
237_R	265_L	0.554	0.74
27_K	38_M	0.553	0.74
87_G	92_Q	0.552	0.74
73_G	82_P	0.551	0.74
181_P	184_N	0.551	0.74
89_D	95_G	0.550	0.74
69_A	77_S	0.549	0.73
26_W	41_F	0.549	0.73
47_I	58_I	0.548	0.73
110_M	113_S	0.547	0.73
33_F	37_M	0.546	0.73
33_F	47_I	0.545	0.73
67_A	77_S	0.540	0.72
35_T	211_W	0.540	0.72
218_A	240_G	0.539	0.72
176_L	227_V	0.537	0.72
25_S	30_Y	0.537	0.72
25_S	39_A	0.536	0.72
33_F	45_W	0.534	0.71
34_M	38_M	0.533	0.71
121_D	124_Q	0.532	0.71
137_P	140_K	0.532	0.71
34_M	39_A	0.532	0.71
246_R	252_P	0.528	0.71
204_G	208_Y	0.525	0.70
162_R	176_L	0.523	0.70
104_E	118_L	0.520	0.69
89_D	97_V	0.517	0.69
74_D	82_P	0.517	0.69
168_V	260_I	0.517	0.69
50_S	60_E	0.514	0.69
152_I	265_L	0.512	0.68
87_G	91_T	0.511	0.68
146_E	150_I	0.510	0.68
70_V	73_G	0.509	0.68
18_S	23_H	0.507	0.67
85_G	92_Q	0.503	0.67
101_P	105_E	0.500	0.66
191_S	263_L	0.500	0.66
88_D	99_K	0.500	0.66
198_D	246_R	0.500	0.66

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2zovA	2	0.539	100	0.542	Contact Map
2zvyA	2	0.5942	100	0.546	Contact Map
3wpwA	2	0.474	100	0.558	Contact Map
3s0yA	4	0.5974	100	0.572	Contact Map
3khnA	2	0.5162	100	0.587	Contact Map
4b62A	1	0.4578	100	0.6	Contact Map
3s06A	3	0.5097	100	0.61	Contact Map
2k1sA	1	0.474	100	0.618	Contact Map
3ldtA	2	0.4578	100	0.621	Contact Map
3oonA	2	0.3604	100	0.636	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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