GREMLIN Database
METF - 5,10-methylenetetrahydrofolate reductase
UniProt: P0AEZ1 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10585
Length: 296 (270)
Sequences: 3763 (2338)
Seq/√Len: 142.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
173_V205_V4.3671.00
258_M289_T3.5251.00
192_L267_E3.2891.00
136_V149_V3.2311.00
103_A139_L3.1881.00
170_K174_D3.1401.00
151_A180_A3.0351.00
168_N171_R3.0251.00
198_C207_I2.8701.00
195_R199_V2.8091.00
233_A237_Q2.6881.00
188_V260_M2.6191.00
148_S179_R2.5881.00
100_R138_L2.5381.00
75_K79_D2.5271.00
192_L263_I2.4091.00
189_E193_R2.3241.00
116_A139_L2.2991.00
78_K85_A2.2761.00
100_R141_E2.2481.00
133_S171_R2.1911.00
104_R142_V2.1781.00
149_V180_A2.1581.00
166_L201_A2.1311.00
84_E113_H2.1151.00
261_V274_F2.1151.00
235_M256_I2.0881.00
163_Q197_R2.0311.00
196_D200_S2.0191.00
167_L171_R1.9581.00
167_L201_A1.9331.00
140_K178_N1.9271.00
262_K266_R1.9041.00
169_L180_A1.9031.00
274_F283_S1.8491.00
161_S164_A1.8401.00
238_M259_D1.7911.00
155_V162_A1.7701.00
149_V177_A1.7531.00
86_A179_R1.7351.00
198_C203_I1.7351.00
184_F224_A1.7241.00
34_T37_M1.7061.00
78_K84_E1.6831.00
211_I257_A1.6801.00
261_V272_F1.6771.00
134_D137_T1.6761.00
188_V263_I1.6361.00
255_N289_T1.6331.00
104_R108_N1.6241.00
251_L285_A1.5941.00
248_T251_L1.5861.00
113_H146_D1.5731.00
131_Y134_D1.5711.00
23_I265_S1.5641.00
221_K236_A1.5631.00
191_Y195_R1.5541.00
160_K164_A1.5521.00
164_A167_L1.5261.00
194_F198_C1.5141.00
193_R196_D1.5121.00
136_V147_I1.5051.00
170_K203_I1.5031.00
285_A288_H1.4761.00
47_R284_Y1.4491.00
51_L284_Y1.4161.00
170_K201_A1.4031.00
180_A205_V1.3921.00
193_R197_R1.3821.00
75_K109_N1.3821.00
25_V287_C1.3761.00
242_L252_V1.3701.00
137_T171_R1.3501.00
191_Y269_V1.3491.00
49_S82_G1.3441.00
245_D248_T1.3181.00
162_A197_R1.3171.00
78_K112_R1.3171.00
130_M134_D1.2971.00
36_E39_Q1.2931.00
221_K240_D1.2851.00
133_S168_N1.2821.00
210_G275_Y1.2771.00
284_Y288_H1.2731.00
195_R267_E1.2721.00
173_V204_D1.2711.00
146_D179_R1.2631.00
39_Q43_N1.2521.00
71_H106_Y1.2501.00
24_N54_K1.2371.00
107_W112_R1.2361.00
250_K281_E1.2231.00
188_V192_L1.2211.00
103_A145_F1.2171.00
263_I267_E1.2121.00
255_N259_D1.2111.00
187_D230_R1.2081.00
195_R207_I1.2051.00
217_F221_K1.2011.00
155_V159_A1.1931.00
219_Q223_F1.1921.00
133_S137_T1.1891.00
96_P141_E1.1851.00
24_N271_D1.1821.00
26_S55_F1.1731.00
146_D178_N1.1721.00
285_A289_T1.1651.00
254_A289_T1.1601.00
251_L255_N1.1480.99
254_A286_I1.1340.99
260_M263_I1.1260.99
133_S175_A1.1230.99
42_W76_G1.1140.99
38_E42_W1.1050.99
100_R142_V1.0990.99
86_A115_V1.0980.99
237_Q240_D1.0930.99
125_S128_P1.0860.99
113_H179_R1.0790.99
171_R175_A1.0780.99
58_V74_I1.0780.99
67_R98_E1.0600.99
173_V180_A1.0510.99
49_S83_L1.0460.99
182_T207_I1.0440.99
222_K226_M1.0380.99
105_D108_N1.0330.99
189_E263_I1.0310.99
253_G257_A1.0310.99
164_A168_N1.0270.99
61_G64_S1.0270.99
87_P106_Y1.0230.99
151_A182_T1.0210.99
46_D81_T1.0190.99
154_E230_R1.0090.99
117_L150_A0.9980.98
211_I261_V0.9970.98
147_I178_N0.9910.98
242_L248_T0.9850.98
40_T44_S0.9790.98
259_D262_K0.9760.98
238_M252_V0.9730.98
60_Y94_A0.9680.98
56_V83_L0.9660.98
184_F223_F0.9620.98
40_T43_N0.9600.98
103_A114_I0.9580.98
197_R201_A0.9490.98
272_F283_S0.9400.98
238_M241_G0.9350.98
43_N46_D0.9350.98
159_A165_D0.9320.98
116_A136_V0.9320.98
107_W143_A0.9280.98
46_D49_S0.9250.98
89_L102_I0.9230.97
140_K147_I0.9210.97
95_T98_E0.9200.97
94_A98_E0.9180.97
30_F33_R0.9150.97
197_R200_S0.9080.97
224_A231_I0.9050.97
125_S129_E0.9050.97
74_I87_P0.8970.97
97_D100_R0.8930.97
35_S69_R0.8880.97
99_L102_I0.8840.97
238_M256_I0.8820.97
190_S193_R0.8820.97
37_M40_T0.8740.97
136_V177_A0.8730.96
114_I145_F0.8680.96
190_S230_R0.8660.96
151_A194_F0.8620.96
47_R280_A0.8610.96
159_A162_A0.8560.96
27_F51_L0.8530.96
218_K221_K0.8510.96
242_L249_R0.8500.96
163_Q201_A0.8460.96
54_K271_D0.8400.96
43_N47_R0.8370.96
29_F77_I0.8360.95
50_S284_Y0.8310.95
217_F240_D0.8300.95
74_I111_I0.8300.95
27_F276_T0.8220.95
22_Q54_K0.8100.95
101_T105_D0.8070.95
137_T141_E0.8040.94
29_F56_V0.8020.94
89_L114_I0.8000.94
244_D247_E0.8000.94
170_K198_C0.7990.94
78_K109_N0.7990.94
223_F226_M0.7920.94
37_M41_L0.7910.94
220_A231_I0.7910.94
199_V204_D0.7840.94
67_R94_A0.7830.94
213_P253_G0.7830.94
45_I77_I0.7800.94
100_R104_R0.7780.93
97_D101_T0.7760.93
238_M255_N0.7750.93
47_R50_S0.7730.93
162_A193_R0.7720.93
21_G24_N0.7720.93
61_G101_T0.7680.93
45_I81_T0.7580.93
260_M264_L0.7560.92
101_T104_R0.7540.92
192_L195_R0.7540.92
166_L170_K0.7530.92
254_A282_M0.7490.92
116_A147_I0.7460.92
191_Y270_K0.7440.92
29_F45_I0.7410.92
256_I260_M0.7400.92
213_P286_I0.7360.91
138_L142_V0.7350.91
169_L203_I0.7340.91
62_A65_G0.7320.91
254_A285_A0.7310.91
187_D234_W0.7300.91
209_P264_L0.7260.91
216_N219_Q0.7260.91
243_D249_R0.7240.91
155_V158_E0.7240.91
247_E251_L0.7240.91
169_L173_V0.7220.91
94_A102_I0.7210.91
77_I85_A0.7200.90
258_M290_L0.7200.90
259_D263_I0.7190.90
42_W81_T0.7180.90
70_T87_P0.7180.90
72_S105_D0.7160.90
174_D198_C0.7140.90
136_V175_A0.7130.90
132_A135_L0.7120.90
234_W259_D0.7070.90
57_S181_I0.7070.90
86_A148_S0.7030.89
107_W144_D0.7020.89
235_M252_V0.7020.89
163_Q200_S0.7010.89
171_R174_D0.7000.89
51_L288_H0.6980.89
138_L141_E0.6980.89
196_D199_V0.6970.89
240_D243_D0.6960.89
152_Y165_D0.6950.89
187_D190_S0.6930.89
29_F41_L0.6920.89
255_N258_M0.6910.89
261_V290_L0.6900.89
198_C202_G0.6890.88
257_A260_M0.6870.88
212_L222_K0.6850.88
58_V77_I0.6830.88
214_V239_F0.6830.88
247_E255_N0.6820.88
276_T283_S0.6820.88
152_Y156_H0.6760.88
250_K279_R0.6760.88
38_E72_S0.6750.87
115_V226_M0.6740.87
99_L138_L0.6730.87
68_D105_D0.6730.87
166_L198_C0.6690.87
59_T273_H0.6670.87
77_I81_T0.6660.87
182_T191_Y0.6660.87
163_Q167_L0.6640.87
139_L145_F0.6610.86
26_S273_H0.6600.86
255_N274_F0.6600.86
254_A257_A0.6540.86
123_P126_G0.6540.86
148_S181_I0.6530.86
59_T275_Y0.6480.85
122_P125_S0.6450.85
188_V267_E0.6440.85
71_H87_P0.6420.85
189_E267_E0.6420.85
173_V203_I0.6410.85
279_R282_M0.6410.85
92_I121_L0.6390.84
191_Y209_P0.6390.84
71_H105_D0.6380.84
166_L197_R0.6380.84
222_K225_D0.6380.84
30_F59_T0.6370.84
214_V235_M0.6350.84
131_Y136_V0.6340.84
27_F48_L0.6330.84
217_F239_F0.6320.84
149_V169_L0.6300.84
115_V148_S0.6290.84
258_M286_I0.6290.84
136_V140_K0.6280.83
120_D156_H0.6250.83
94_A99_L0.6220.83
113_H148_S0.6200.83
23_I271_D0.6190.83
209_P270_K0.6160.82
60_Y90_T0.6160.82
106_Y114_I0.6150.82
217_F236_A0.6150.82
91_C94_A0.6130.82
233_A236_A0.6100.82
156_H165_D0.6100.82
186_F257_A0.6080.81
61_G65_G0.6070.81
252_V256_I0.6050.81
42_W46_D0.6010.81
162_A166_L0.5970.80
151_A169_L0.5970.80
234_W256_I0.5950.80
24_N208_I0.5930.80
265_S272_F0.5930.80
184_F277_L0.5920.80
38_E73_I0.5880.79
159_A164_A0.5880.79
132_A150_A0.5870.79
98_E102_I0.5860.79
189_E234_W0.5810.78
48_L210_G0.5810.78
281_E285_A0.5810.78
29_F34_T0.5790.78
195_R270_K0.5770.78
87_P111_I0.5770.78
68_D108_N0.5750.78
91_C135_L0.5720.77
61_G66_E0.5680.77
265_S290_L0.5660.77
231_I236_A0.5640.76
76_G81_T0.5640.76
252_V255_N0.5620.76
137_T175_A0.5620.76
126_G129_E0.5610.76
216_N223_F0.5590.76
255_N262_K0.5590.76
189_E192_L0.5580.76
215_S282_M0.5580.76
117_L183_Q0.5580.76
187_D260_M0.5580.76
179_R208_I0.5540.75
29_F74_I0.5540.75
33_R65_G0.5520.75
261_V265_S0.5500.75
57_S86_A0.5490.74
223_F227_T0.5490.74
34_T41_L0.5490.74
281_E288_H0.5490.74
188_V264_L0.5440.74
131_Y168_N0.5430.74
102_I106_Y0.5400.73
60_Y92_I0.5400.73
65_G229_V0.5400.73
152_Y158_E0.5390.73
191_Y264_L0.5370.73
40_T279_R0.5370.73
95_T128_P0.5360.73
27_F53_P0.5350.73
192_L196_D0.5340.72
49_S81_T0.5340.72
68_D72_S0.5340.72
55_F179_R0.5330.72
90_T212_L0.5320.72
236_A240_D0.5320.72
38_E45_I0.5290.72
242_L245_D0.5280.72
185_F191_Y0.5260.71
51_L287_C0.5250.71
118_R158_E0.5250.71
66_E121_L0.5240.71
274_F286_I0.5220.71
195_R268_G0.5200.70
112_R144_D0.5190.70
258_M287_C0.5180.70
254_A258_M0.5170.70
107_W145_F0.5160.70
262_K289_T0.5140.70
88_H183_Q0.5140.70
174_D177_A0.5130.69
96_P100_R0.5130.69
117_L273_H0.5120.69
57_S278_N0.5120.69
105_D109_N0.5100.69
184_F212_L0.5080.69
58_V70_T0.5080.69
34_T40_T0.5070.69
63_N66_E0.5050.68
98_E101_T0.5050.68
36_E221_K0.5040.68
223_F229_V0.5030.68
182_T279_R0.5030.68
220_A224_A0.5030.68
152_Y275_Y0.5030.68
217_F242_L0.5030.68
217_F243_D0.5020.68
174_D200_S0.5000.67
107_W141_E0.5000.67
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3fstA 4 0.9662 100 0.086 Contact Map
3aptA 2 0.9561 100 0.097 Contact Map
3ijdA 2 0.8885 100 0.267 Contact Map
4ht3A 1 0.8041 96.9 0.909 Contact Map
2p10A 5 0.777 96.5 0.913 Contact Map
1rd5A 6 0.7804 96.3 0.915 Contact Map
3vndA 3 0.7872 96 0.918 Contact Map
3navA 2 0.7973 95.8 0.919 Contact Map
1vc4A 2 0.7399 94.5 0.925 Contact Map
2ekcA 1 0.777 94.5 0.925 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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