GREMLIN Database
K1PF - 1-phosphofructokinase
UniProt: P0AEW9 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10337
Length: 312 (283)
Sequences: 22537 (16839)
Seq/√Len: 1001.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
54_I263_Y3.1701.00
136_C165_I2.7211.00
57_T80_A2.5571.00
71_Q86_V2.5031.00
270_S273_H2.4741.00
231_W275_L2.4621.00
224_L230_L2.4571.00
264_G269_E2.3461.00
229_A257_M2.2651.00
7_T132_F2.2371.00
155_T180_A2.2141.00
49_L54_I2.1711.00
213_R232_V2.1121.00
68_D86_V2.0731.00
157_L162_P2.0551.00
188_R224_L1.9971.00
51_D77_L1.9891.00
64_K87_Q1.9511.00
165_I262_I1.9341.00
238_W272_E1.9221.00
295_D298_Q1.9201.00
264_G277_L1.9191.00
154_M164_I1.9061.00
8_I46_A1.9041.00
256_S281_V1.8431.00
134_M163_C1.8401.00
126_L156_R1.8351.00
269_E273_H1.8261.00
72_Q76_E1.7981.00
185_K258_V1.7961.00
47_K77_L1.7791.00
267_M296_R1.7681.00
48_V259_G1.7661.00
231_W238_W1.7601.00
75_S81_N1.7591.00
229_A279_T1.7581.00
163_C182_W1.7501.00
182_W218_A1.7351.00
209_A232_V1.7311.00
224_L239_I1.7121.00
211_A215_Q1.7101.00
288_Q298_Q1.6961.00
253_A286_V1.6931.00
272_E276_R1.6851.00
151_T176_G1.6741.00
73_L76_E1.6671.00
261_L274_T1.6631.00
221_V261_L1.6571.00
230_L239_I1.6401.00
71_Q75_S1.6291.00
134_M165_I1.6131.00
192_E198_K1.6121.00
152_D155_T1.6071.00
183_L219_H1.5891.00
156_R160_Q1.5701.00
282_A286_V1.5681.00
49_L263_Y1.5381.00
211_A214_E1.5311.00
134_M262_I1.5231.00
151_T155_T1.5221.00
12_P60_G1.5221.00
68_D72_Q1.5181.00
64_K68_D1.5181.00
249_S290_N1.5071.00
260_G281_V1.5041.00
219_H233_N1.4971.00
243_P280_A1.4961.00
233_N236_G1.4921.00
36_L69_G1.4871.00
240_A275_L1.4811.00
204_D207_E1.4761.00
231_W271_S1.4701.00
38_A70_F1.4531.00
224_L227_E1.4331.00
210_H214_E1.4331.00
130_G160_Q1.4321.00
158_R182_W1.4231.00
206_I210_H1.4201.00
156_R159_S1.4121.00
167_D223_S1.4061.00
152_D156_R1.3991.00
260_G277_L1.3801.00
163_C183_L1.3771.00
197_R211_A1.3441.00
243_P276_R1.3391.00
240_A279_T1.3311.00
14_Y69_G1.3171.00
181_P184_V1.3131.00
55_D80_A1.3081.00
206_I237_E1.3061.00
43_I74_F1.3051.00
158_R180_A1.2981.00
245_V287_S1.2951.00
168_S186_P1.2931.00
163_C218_A1.2891.00
67_Q90_T1.2861.00
6_A49_L1.2701.00
229_A275_L1.2591.00
243_P279_T1.2481.00
219_H265_L1.2331.00
38_A73_L1.2291.00
224_L228_G1.2231.00
223_S257_M1.2231.00
247_V286_V1.2101.00
261_L265_L1.2091.00
16_L34_T1.2071.00
73_L77_L1.2071.00
185_K223_S1.2071.00
213_R234_A1.2061.00
273_H276_R1.2031.00
219_H261_L1.1981.00
170_R173_L1.1981.00
263_Y267_M1.1951.00
219_H271_S1.1951.00
71_Q84_Q1.1921.00
210_H232_V1.1791.00
64_K86_V1.1791.00
21_P32_K1.1771.00
197_R207_E1.1761.00
72_Q75_S1.1721.00
245_V286_V1.1721.00
69_G72_Q1.1711.00
52_L260_G1.1671.00
135_V157_L1.1661.00
260_G278_A1.1591.00
50_K79_I1.1461.00
68_D71_Q1.1341.00
155_T159_S1.1291.00
261_L278_A1.1291.00
264_G274_T1.1211.00
137_V164_I1.1181.00
188_R227_E1.1171.00
49_L56_V1.1111.00
45_V136_C1.1091.00
284_L287_S1.1081.00
243_P283_A1.1071.00
46_A56_V1.1041.00
21_P99_K1.1031.00
16_L36_L1.0981.00
207_E210_H1.0881.00
183_L261_L1.0801.00
61_F120_R1.0751.00
284_L288_Q1.0731.00
136_C262_I1.0701.00
54_I266_L1.0671.00
19_F33_T1.0631.00
219_H231_W1.0611.00
170_R174_V1.0471.00
247_V250_T1.0361.00
62_L67_Q1.0361.00
47_K51_D1.0321.00
264_G267_M1.0321.00
191_L222_I1.0321.00
126_L160_Q1.0241.00
71_Q85_V1.0221.00
144_G148_E1.0211.00
54_I267_M1.0211.00
127_S130_G1.0181.00
62_L71_Q1.0131.00
218_A234_A1.0091.00
279_T282_A1.0051.00
50_K78_G1.0041.00
48_V256_S1.0011.00
57_T128_W1.0011.00
12_P70_F0.9991.00
183_L265_L0.9971.00
232_V237_E0.9961.00
209_A222_I0.9891.00
261_L271_S0.9871.00
242_P279_T0.9841.00
185_K261_L0.9821.00
191_L208_A0.9821.00
210_H237_E0.9791.00
57_T83_F0.9791.00
81_N84_Q0.9661.00
135_V162_P0.9611.00
38_A69_G0.9581.00
57_T132_F0.9571.00
148_E152_D0.9531.00
50_K55_D0.9521.00
256_S285_A0.9501.00
276_R280_A0.9471.00
8_I42_G0.9461.00
166_F181_P0.9451.00
69_G73_L0.9411.00
31_V106_F0.9401.00
195_A208_A0.9301.00
227_E239_I0.9271.00
236_G272_E0.9251.00
18_G94_V0.9161.00
155_T176_G0.9131.00
274_T278_A0.9121.00
136_C258_V0.9111.00
188_R192_E0.9091.00
52_L281_V0.9081.00
137_V150_F0.9071.00
263_Y296_R0.9061.00
17_V37_H0.9051.00
187_N190_E0.9011.00
263_Y277_L0.8971.00
137_V154_M0.8951.00
16_L92_I0.8951.00
129_L132_F0.8911.00
49_L259_G0.8911.00
228_G242_P0.8881.00
19_F35_G0.8881.00
247_V287_S0.8861.00
234_A237_E0.8841.00
71_Q81_N0.8811.00
270_S274_T0.8791.00
65_D90_T0.8691.00
132_F162_P0.8681.00
246_D287_S0.8681.00
167_D185_K0.8651.00
126_L159_S0.8651.00
12_P58_V0.8601.00
210_H213_R0.8601.00
265_L274_T0.8581.00
209_A220_V0.8581.00
63_G90_T0.8561.00
62_L84_Q0.8551.00
256_S293_I0.8511.00
24_E27_E0.8471.00
7_T59_G0.8461.00
256_S260_G0.8441.00
43_I292_G0.8361.00
70_F90_T0.8311.00
238_W275_L0.8301.00
9_T153_W0.8291.00
20_C23_I0.8261.00
207_E211_A0.8261.00
12_P43_I0.8231.00
45_V258_V0.8201.00
257_M279_T0.8141.00
240_A272_E0.8081.00
240_A276_R0.8051.00
124_D127_S0.8051.00
186_P191_L0.7991.00
248_V289_S0.7991.00
96_L106_F0.7981.00
269_E277_L0.7951.00
15_D39_A0.7861.00
9_T135_V0.7861.00
167_D186_P0.7861.00
61_F85_V0.7851.00
117_D120_R0.7741.00
50_K56_V0.7721.00
25_R102_E0.7721.00
256_S282_A0.7701.00
172_A175_A0.7691.00
245_V283_A0.7681.00
206_I239_I0.7681.00
260_G264_G0.7661.00
178_K181_P0.7641.00
129_L162_P0.7621.00
165_I185_K0.7601.00
46_A58_V0.7601.00
118_W149_A0.7591.00
62_L86_V0.7561.00
114_T117_D0.7531.00
57_T82_R0.7471.00
171_E175_A0.7461.00
22_E27_E0.7451.00
154_M180_A0.7451.00
149_A153_W0.7441.00
221_V231_W0.7421.00
91_R96_L0.7391.00
233_N271_S0.7391.00
116_A119_E0.7371.00
126_L130_G0.7351.00
279_T283_A0.7351.00
95_K105_D0.7331.00
191_L212_L0.7321.00
233_N238_W0.7311.00
213_R233_N0.7261.00
52_L263_Y0.7221.00
226_A244_S0.7181.00
176_G179_A0.7161.00
277_L299_L0.7151.00
93_N96_L0.7151.00
52_L299_L0.7141.00
277_L296_R0.7121.00
151_T180_A0.7081.00
209_A213_R0.7051.00
47_K292_G0.7041.00
134_M266_L0.6991.00
206_I232_V0.6991.00
13_A91_R0.6921.00
90_T93_N0.6911.00
83_F86_V0.6901.00
6_A266_L0.6901.00
282_A285_A0.6901.00
7_T57_T0.6881.00
257_M278_A0.6881.00
52_L277_L0.6881.00
280_A284_L0.6851.00
73_L292_G0.6851.00
197_R208_A0.6811.00
184_V220_V0.6771.00
206_I230_L0.6731.00
228_G239_I0.6711.00
165_I183_L0.6661.00
165_I258_V0.6661.00
60_G84_Q0.6611.00
55_D133_D0.6611.00
35_G289_S0.6591.00
269_E274_T0.6581.00
83_F128_W0.6571.00
103_V107_N0.6571.00
41_K169_S0.6561.00
157_L164_I0.6551.00
128_W132_F0.6551.00
14_Y36_L0.6551.00
252_G293_I0.6521.00
281_V299_L0.6441.00
151_T154_M0.6441.00
135_V153_W0.6431.00
148_E151_T0.6411.00
257_M275_L0.6351.00
147_P151_T0.6341.00
104_T107_N0.6321.00
39_A44_N0.6311.00
229_A240_A0.6311.00
19_F289_S0.6281.00
167_D255_D0.6281.00
213_R235_S0.6211.00
275_L279_T0.6191.00
46_A79_I0.6151.00
205_V224_L0.6141.00
257_M282_A0.6131.00
98_E102_E0.6121.00
170_R175_A0.6101.00
133_D162_P0.6091.00
7_T128_W0.6071.00
55_D266_L0.6051.00
66_N69_G0.6051.00
294_T299_L0.6001.00
178_K217_I0.5991.00
43_I58_V0.5971.00
68_D75_S0.5961.00
284_L298_Q0.5961.00
52_L259_G0.5951.00
208_A212_L0.5941.00
113_V116_A0.5931.00
268_R274_T0.5921.00
119_E152_D0.5911.00
134_M183_L0.5911.00
171_E174_V0.5901.00
284_L299_L0.5901.00
77_L295_D0.5901.00
145_V148_E0.5891.00
177_L181_P0.5861.00
240_A243_P0.5851.00
93_N107_N0.5801.00
18_G34_T0.5771.00
6_A134_M0.5761.00
92_I96_L0.5751.00
133_D163_C0.5741.00
217_I220_V0.5721.00
23_I104_T0.5681.00
206_I209_A0.5681.00
213_R217_I0.5671.00
233_N237_E0.5661.00
49_L262_I0.5641.00
175_A215_Q0.5641.00
25_R98_E0.5631.00
259_G263_Y0.5631.00
45_V262_I0.5631.00
164_I181_P0.5581.00
221_V257_M0.5581.00
191_L205_V0.5581.00
273_H277_L0.5541.00
151_T174_V0.5461.00
38_A292_G0.5421.00
185_K221_V0.5411.00
128_W131_Q0.5411.00
213_R220_V0.5401.00
157_L161_C0.5391.00
226_A247_V0.5391.00
44_N291_V0.5391.00
18_G31_V0.5371.00
191_L220_V0.5331.00
74_F79_I0.5321.00
96_L104_T0.5321.00
197_R204_D0.5321.00
50_K77_L0.5311.00
221_V275_L0.5311.00
205_V208_A0.5301.00
271_S274_T0.5291.00
42_G46_A0.5281.00
102_E105_D0.5281.00
59_G125_S0.5281.00
165_I265_L0.5271.00
181_P217_I0.5271.00
213_R216_G0.5261.00
127_S131_Q0.5261.00
64_K88_G0.5251.00
189_R193_I0.5231.00
95_K251_V0.5231.00
108_F111_F0.5211.00
85_V120_R0.5211.00
19_F99_K0.5211.00
45_V259_G0.5201.00
195_A211_A0.5191.00
134_M265_L0.5191.00
179_A216_G0.5181.00
271_S275_L0.5161.00
265_L268_R0.5161.00
30_L107_N0.5151.00
154_M177_L0.5151.00
93_N105_D0.5111.00
28_V41_K0.5111.00
43_I47_K0.5101.00
90_T95_K0.5091.00
179_A215_Q0.5081.00
125_S128_W0.5061.00
91_R95_K0.5061.00
82_R85_V0.5051.00
116_A120_R0.5041.00
153_W156_R0.5041.00
105_D251_V0.5031.00
146_S150_F0.5021.00
251_V291_V0.5021.00
191_L232_V0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2ajrA 2 0.9904 100 0.186 Contact Map
2jg1A 2 0.9904 100 0.187 Contact Map
3vasA 1 0.9519 100 0.202 Contact Map
2f02A 2 0.9872 100 0.205 Contact Map
3ie7A 2 0.9712 100 0.21 Contact Map
2abqA 2 0.9712 100 0.217 Contact Map
2jg5A 1 0.9712 100 0.218 Contact Map
2fv7A 2 0.9487 100 0.223 Contact Map
3umoA 4 0.9744 100 0.223 Contact Map
2rbcA 2 0.9455 100 0.223 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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