GREMLIN Database
HYFE - Hydrogenase-4 component E
UniProt: P0AEW1 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG14213
Length: 216 (210)
Sequences: 397 (294)
Seq/√Len: 20.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
106_V133_G4.0051.00
49_S58_L3.3541.00
22_I102_A3.2951.00
46_A61_S3.1571.00
39_L69_V2.7470.99
184_D189_V2.5870.98
98_M144_S2.5600.98
10_L40_V2.4930.97
69_V215_K2.4610.97
38_S183_T2.3640.96
177_V180_G2.2420.95
145_Q148_I2.2140.95
190_I194_L2.1400.94
184_D214_L2.0950.93
155_Y172_R2.0900.93
46_A55_E2.0580.92
21_V141_C1.9890.91
90_K152_I1.9610.90
42_V65_F1.9560.90
61_S205_T1.9060.89
14_M192_M1.8720.88
46_A82_N1.8580.88
65_F70_L1.8330.87
62_A82_N1.8330.87
17_T39_L1.8310.87
169_L176_L1.7960.86
146_R211_L1.7940.86
163_H166_L1.7790.85
36_C39_L1.7610.85
34_Y156_C1.7210.83
145_Q151_Q1.6830.82
34_Y183_T1.6610.81
155_Y183_T1.6370.80
59_I71_L1.6160.79
15_M71_L1.6090.79
110_A138_G1.5600.77
17_T107_L1.5580.76
173_A183_T1.5460.76
180_G184_D1.5280.75
101_L140_L1.5180.75
134_H155_Y1.5180.75
28_R149_L1.5150.74
19_L102_A1.5050.74
10_L43_S1.4920.73
21_V157_L1.4710.72
59_I100_L1.4580.71
41_L190_I1.4320.70
72_V148_I1.4240.70
139_L142_I1.4210.69
9_N186_I1.3800.67
39_L58_L1.3800.67
135_F161_G1.3780.67
44_I49_S1.3720.67
109_C195_L1.3700.67
6_I169_L1.3560.66
39_L43_S1.3510.65
191_V203_H1.3390.65
97_M190_I1.3380.65
45_F169_L1.3320.64
149_L177_V1.2970.62
147_N156_C1.2930.62
8_N93_F1.2790.61
151_Q178_E1.2780.61
45_F196_A1.2740.61
31_C97_M1.2740.61
198_K203_H1.2640.60
27_Y31_C1.2560.60
62_A66_I1.2470.59
6_I23_S1.2370.58
110_A117_K1.2370.58
77_T81_R1.2280.58
155_Y195_L1.2180.57
88_P145_Q1.2150.57
26_S147_N1.2140.57
71_L74_L1.2090.57
26_S68_K1.2040.56
46_A62_A1.2020.56
66_I71_L1.1990.56
64_A158_M1.1830.55
38_S180_G1.1810.55
28_R32_G1.1770.55
22_I137_L1.1760.54
142_I154_G1.1710.54
38_S182_A1.1610.54
130_V179_I1.1480.53
138_G157_L1.1460.53
38_S90_K1.1430.52
175_E183_T1.1210.51
140_L176_L1.1160.51
157_L163_H1.1140.50
75_I150_R1.1130.50
81_R136_L1.1110.50
128_L187_F1.1070.50
175_E186_I1.0980.49
150_R156_C1.0960.49
25_K109_C1.0950.49
50_C58_L1.0870.49
13_L40_V1.0720.48
17_T194_L1.0630.47
19_L108_L1.0550.47
147_N199_I1.0530.46
41_L177_V1.0510.46
30_S67_T1.0500.46
68_K174_P1.0410.46
159_E167_A1.0330.45
101_L153_F1.0330.45
38_S69_V1.0320.45
163_H180_G1.0310.45
25_K141_C1.0310.45
138_G161_G1.0270.45
105_I172_R1.0220.44
192_M200_W1.0200.44
27_Y215_K1.0160.44
131_A165_V1.0120.44
68_K199_I1.0090.44
51_L71_L1.0080.44
11_A162_S1.0070.43
68_K131_A1.0050.43
12_G167_A1.0020.43
43_S70_L0.9950.43
180_G214_L0.9950.43
212_T215_K0.9860.42
81_R116_V0.9830.42
166_L174_P0.9760.42
188_A198_K0.9740.41
112_V130_V0.9710.41
106_V139_L0.9690.41
58_L128_L0.9680.41
21_V188_A0.9670.41
69_V94_G0.9640.41
206_L212_T0.9640.41
133_G174_P0.9570.40
148_I194_L0.9550.40
25_K131_A0.9520.40
28_R77_T0.9490.40
110_A144_S0.9350.39
93_F114_Q0.9320.39
14_M134_H0.9290.39
177_V181_I0.9260.38
148_I203_H0.9230.38
17_T213_A0.9220.38
127_A165_V0.9150.38
74_L101_L0.9140.38
76_M201_R0.8950.36
31_C35_A0.8680.35
132_L211_L0.8650.35
30_S153_F0.8620.35
71_L75_I0.8610.34
88_P208_V0.8580.34
55_E62_A0.8540.34
60_W155_Y0.8440.33
186_I195_L0.8430.33
197_R204_G0.8390.33
60_W196_A0.8370.33
181_I199_I0.8360.33
68_K187_F0.8270.32
26_S195_L0.8260.32
170_A177_V0.8220.32
196_A206_L0.8200.32
139_L187_F0.8190.32
156_C183_T0.8160.32
155_Y188_A0.8110.32
31_C183_T0.8080.31
25_K115_P0.8030.31
73_P161_G0.7970.31
56_Q187_F0.7960.31
7_V17_T0.7960.31
135_F162_S0.7940.31
35_A70_L0.7910.30
110_A133_G0.7900.30
57_L194_L0.7860.30
26_S199_I0.7800.30
47_T62_A0.7790.30
30_S150_R0.7780.30
50_C63_S0.7710.29
183_T192_M0.7680.29
98_M140_L0.7660.29
34_Y38_S0.7640.29
48_L61_S0.7600.29
33_F71_L0.7600.29
26_S161_G0.7590.29
211_L214_L0.7590.29
46_A66_I0.7590.29
46_A79_A0.7580.29
67_T155_Y0.7570.29
43_S55_E0.7510.28
159_E182_A0.7510.28
46_A215_K0.7460.28
134_H138_G0.7460.28
25_K41_L0.7420.28
37_Q184_D0.7420.28
75_I156_C0.7370.28
94_G97_M0.7350.27
37_Q53_A0.7350.27
28_R31_C0.7340.27
44_I99_A0.7290.27
17_T144_S0.7230.27
74_L181_I0.7230.27
166_L193_V0.7190.27
116_V175_E0.7140.26
7_V171_W0.7130.26
68_K159_E0.7110.26
39_L93_F0.7080.26
176_L211_L0.7060.26
107_L133_G0.7060.26
27_Y119_P0.7040.26
25_K106_V0.7040.26
43_S53_A0.7030.26
59_I67_T0.7010.26
133_G140_L0.7010.26
161_G193_V0.6970.26
181_I189_V0.6920.25
161_G207_D0.6920.25
162_S214_L0.6920.25
137_L162_S0.6900.25
65_F95_P0.6890.25
142_I204_G0.6890.25
55_E59_I0.6870.25
135_F142_I0.6790.25
42_V181_I0.6770.25
139_L156_C0.6770.25
98_M176_L0.6760.24
126_P129_A0.6690.24
69_V109_C0.6690.24
95_P101_L0.6620.24
40_V212_T0.6620.24
68_K207_D0.6570.24
111_F143_V0.6570.24
9_N120_M0.6550.23
180_G215_K0.6550.23
208_V214_L0.6490.23
35_A190_I0.6480.23
34_Y153_F0.6470.23
94_G155_Y0.6460.23
113_V188_A0.6460.23
110_A212_T0.6400.23
84_P154_G0.6390.23
6_I100_L0.6390.23
66_I132_L0.6380.23
152_I169_L0.6320.22
68_K208_V0.6270.22
80_A194_L0.6250.22
142_I193_V0.6220.22
36_C132_L0.6220.22
79_A190_I0.6210.22
208_V212_T0.6200.22
112_V212_T0.6200.22
49_S202_T0.6200.22
88_P151_Q0.6160.22
192_M206_L0.6160.22
21_V195_L0.6140.22
60_W71_L0.6130.22
156_C162_S0.6100.21
68_K83_I0.6050.21
46_A146_R0.6030.21
60_W182_A0.5960.21
28_R66_I0.5920.21
156_C165_V0.5880.20
76_M115_P0.5850.20
18_S155_Y0.5850.20
10_L212_T0.5820.20
103_A211_L0.5810.20
78_Y149_L0.5800.20
189_V214_L0.5750.20
45_F152_I0.5740.20
106_V109_C0.5710.20
69_V110_A0.5690.20
37_Q145_Q0.5660.20
43_S105_I0.5650.20
130_V180_G0.5610.19
91_A112_V0.5610.19
122_T171_W0.5580.19
91_A98_M0.5580.19
36_C78_Y0.5570.19
88_P98_M0.5570.19
27_Y181_I0.5540.19
57_L102_A0.5520.19
15_M70_L0.5520.19
18_S106_V0.5520.19
59_I189_V0.5510.19
29_L181_I0.5470.19
133_G195_L0.5470.19
126_P155_Y0.5450.19
48_L151_Q0.5440.19
8_N16_L0.5430.19
115_P177_V0.5420.19
57_L200_W0.5410.19
58_L164_L0.5370.18
20_F131_A0.5350.18
48_L131_A0.5340.18
12_G60_W0.5340.18
70_L101_L0.5320.18
17_T66_I0.5310.18
64_A168_L0.5300.18
147_N170_A0.5280.18
102_A106_V0.5280.18
36_C170_A0.5260.18
128_L143_V0.5240.18
37_Q160_N0.5210.18
110_A158_M0.5190.18
14_M45_F0.5170.18
69_V82_N0.5170.18
142_I172_R0.5170.18
56_Q176_L0.5160.18
16_L193_V0.5150.18
170_A180_G0.5150.18
26_S193_V0.5140.18
78_Y141_C0.5110.17
40_V71_L0.5100.17
101_L115_P0.5080.17
18_S60_W0.5060.17
151_Q187_F0.5050.17
14_M136_L0.5040.17
32_G191_V0.5040.17
78_Y125_K0.5000.17
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4he8K 1 0.4167 97 0.878 Contact Map
3rkoK 1 0.4398 96.6 0.884 Contact Map
3rkoN 1 0.9537 45.1 0.941 Contact Map
3rkoM 1 0.9213 25.7 0.948 Contact Map
2qi9A 2 0.5787 17.1 0.952 Contact Map
4he8N 1 0.8981 15.3 0.953 Contact Map
4g1uA 2 0.5787 15 0.953 Contact Map
4he8M 1 0.9213 12.9 0.955 Contact Map
2nq2A 2 0.5833 11.1 0.956 Contact Map
3w4tA 1 0.3843 5.8 0.961 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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