GREMLIN Database
HISJ - Histidine-binding periplasmic protein
UniProt: P0AEU0 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG12124
Length: 260 (228)
Sequences: 21512 (15033)
Seq/√Len: 995.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
31_G86_I3.8141.00
137_G177_I3.5881.00
27_N68_T3.5331.00
62_R238_E2.8571.00
145_E161_S2.3541.00
162_Y171_D2.3231.00
80_S86_I2.2851.00
164_G167_N2.2391.00
29_R68_T2.2111.00
107_D237_A2.1951.00
95_I218_G2.1711.00
27_N66_Q2.1151.00
95_I100_Q2.1141.00
246_E250_K2.1041.00
97_E100_Q2.0801.00
145_E149_N2.0781.00
29_R70_V2.0371.00
244_T247_K2.0301.00
82_K102_E1.9791.00
237_A241_A1.9681.00
60_C67_C1.9631.00
58_E61_K1.9541.00
135_R160_V1.9331.00
229_R233_N1.8981.00
104_A229_R1.8891.00
43_N49_V1.8761.00
221_L229_R1.8761.00
62_R235_A1.8761.00
103_I220_G1.8481.00
182_Q187_A1.8401.00
107_D233_N1.8131.00
221_L232_L1.7901.00
127_T130_S1.7901.00
168_I180_A1.7591.00
226_N230_E1.7251.00
58_E244_T1.7121.00
80_S85_K1.6591.00
171_D176_R1.6341.00
30_I56_A1.6331.00
117_V187_A1.6251.00
135_R158_E1.6051.00
90_M219_M1.5741.00
58_E239_M1.5721.00
140_Q163_Q1.5631.00
43_N47_E1.5491.00
58_E62_R1.5341.00
119_A175_G1.5001.00
72_N80_S1.4811.00
222_R225_D1.4781.00
230_E234_K1.4691.00
42_K71_E1.4561.00
106_T233_N1.4331.00
119_A122_S1.4111.00
146_T150_E1.3901.00
78_I103_I1.3671.00
120_K199_D1.3661.00
111_A216_G1.3641.00
62_R242_D1.3621.00
77_L89_I1.3561.00
41_S49_V1.3391.00
231_A234_K1.3331.00
104_A224_E1.3321.00
121_N201_K1.3321.00
20_A24_I1.3191.00
173_T199_D1.3151.00
54_D251_K1.3141.00
147_F151_H1.3111.00
171_D177_I1.2781.00
57_K69_F1.2771.00
53_I57_K1.2711.00
34_P71_E1.2671.00
63_I235_A1.2651.00
135_R178_D1.2641.00
149_N161_S1.2531.00
184_E188_S1.2501.00
59_L235_A1.2411.00
93_L220_G1.2371.00
163_Q167_N1.2311.00
84_K222_R1.2121.00
52_D109_L1.2081.00
114_S144_Q1.2051.00
134_K178_D1.1981.00
170_S174_A1.1911.00
30_I88_A1.1901.00
116_L179_A1.1881.00
115_R184_E1.1861.00
56_A90_M1.1841.00
238_E242_D1.1801.00
51_F253_F1.1701.00
104_A226_N1.1621.00
79_P83_A1.1461.00
118_V179_A1.1391.00
138_V145_E1.1341.00
115_R202_F1.1191.00
89_I93_L1.1191.00
137_G162_Y1.1171.00
136_V179_A1.1171.00
51_F248_L1.1161.00
132_K156_G1.1121.00
98_K101_Q1.1111.00
19_A22_A1.1031.00
21_F25_P1.0991.00
235_A238_E1.0961.00
226_N229_R1.0951.00
240_R245_Y1.0951.00
26_Q65_T1.0931.00
120_K201_K1.0921.00
72_N86_I1.0921.00
235_A239_M1.0881.00
79_P82_K1.0861.00
231_A235_A1.0831.00
20_A23_A1.0691.00
162_Y168_I1.0671.00
170_S173_T1.0481.00
57_K61_K1.0451.00
55_L239_M1.0331.00
172_L180_A1.0281.00
172_L200_Y1.0201.00
65_T231_A1.0191.00
73_P76_A1.0181.00
56_A69_F1.0141.00
139_L142_T1.0121.00
56_A67_C1.0081.00
172_L177_I1.0081.00
47_E57_K1.0011.00
244_T248_L0.9911.00
51_F249_A0.9911.00
122_S125_Q0.9841.00
17_A227_E0.9831.00
42_K46_G0.9791.00
54_D252_Y0.9761.00
30_I67_C0.9761.00
25_P29_R0.9711.00
55_L245_Y0.9681.00
49_V54_D0.9621.00
118_V203_G0.9621.00
234_K238_E0.9601.00
146_T149_N0.9541.00
54_D57_K0.9521.00
22_A26_Q0.9501.00
167_N170_S0.9491.00
239_M244_T0.9331.00
88_A221_L0.9301.00
104_A223_K0.9281.00
245_Y249_A0.9261.00
82_K103_I0.9221.00
51_F245_Y0.9221.00
88_A232_L0.9211.00
227_E231_A0.9201.00
233_N236_F0.9111.00
78_I93_L0.9071.00
137_G168_I0.9061.00
22_A65_T0.9061.00
62_R244_T0.8981.00
22_A25_P0.8951.00
48_L53_I0.8861.00
59_L232_L0.8811.00
96_T99_R0.8801.00
173_T195_P0.8791.00
140_Q161_S0.8781.00
118_V122_S0.8721.00
37_A41_S0.8711.00
94_S215_V0.8711.00
125_Q130_S0.8681.00
195_P198_K0.8681.00
247_K250_K0.8661.00
153_A156_G0.8591.00
39_F110_Y0.8591.00
99_R105_F0.8521.00
97_E101_Q0.8461.00
88_A219_M0.8451.00
74_L77_L0.8451.00
106_T232_L0.8351.00
34_P48_L0.8341.00
18_T21_F0.8331.00
147_F150_E0.8331.00
107_D240_R0.8321.00
153_A157_I0.8301.00
116_L181_F0.8291.00
112_A185_V0.8271.00
100_Q223_K0.8261.00
76_A80_S0.8151.00
233_N237_A0.8121.00
129_E132_K0.8091.00
249_A253_F0.8071.00
152_W157_I0.8001.00
165_Q182_Q0.7931.00
166_D170_S0.7891.00
247_K251_K0.7851.00
238_E241_A0.7821.00
105_F220_G0.7811.00
42_K48_L0.7791.00
189_E194_Q0.7741.00
58_E235_A0.7741.00
28_I88_A0.7691.00
32_T91_S0.7691.00
19_A23_A0.7681.00
56_A219_M0.7681.00
28_I65_T0.7671.00
62_R239_M0.7621.00
152_W156_G0.7601.00
17_A21_F0.7581.00
89_I220_G0.7571.00
246_E249_A0.7551.00
17_A20_A0.7531.00
59_L63_I0.7531.00
115_R204_G0.7531.00
32_T53_I0.7521.00
123_D201_K0.7311.00
32_T69_F0.7281.00
113_D184_E0.7251.00
95_I108_K0.7251.00
56_A88_A0.7211.00
125_Q201_K0.7211.00
112_A184_E0.7191.00
169_Y173_T0.7171.00
57_K67_C0.7161.00
117_V200_Y0.7161.00
239_M242_D0.7111.00
160_V171_D0.7051.00
28_I60_C0.7051.00
106_T221_L0.7031.00
63_I231_A0.7021.00
38_P41_S0.7021.00
162_Y177_I0.7011.00
70_V85_K0.6961.00
227_E230_E0.6961.00
23_A26_Q0.6941.00
30_I69_F0.6931.00
131_L179_A0.6911.00
29_R87_D0.6891.00
81_L89_I0.6851.00
150_E153_A0.6851.00
19_A25_P0.6821.00
118_V201_K0.6801.00
148_G159_I0.6801.00
200_Y203_G0.6791.00
72_N77_L0.6791.00
84_K224_E0.6781.00
94_S99_R0.6781.00
59_L90_M0.6771.00
234_K237_A0.6771.00
76_A79_P0.6751.00
78_I82_K0.6751.00
167_N171_D0.6731.00
124_I134_K0.6631.00
239_M245_Y0.6611.00
58_E248_L0.6601.00
34_P40_E0.6601.00
115_R187_A0.6581.00
41_S252_Y0.6571.00
16_S21_F0.6551.00
104_A225_D0.6551.00
135_R177_I0.6541.00
137_G171_D0.6501.00
33_D74_L0.6481.00
225_D228_L0.6471.00
169_Y200_Y0.6471.00
18_T22_A0.6461.00
55_L236_F0.6461.00
36_Y92_S0.6431.00
27_N65_T0.6401.00
219_M232_L0.6391.00
239_M243_G0.6351.00
81_L86_I0.6331.00
95_I105_F0.6311.00
230_E233_N0.6281.00
188_S193_K0.6241.00
117_V169_Y0.6231.00
33_D40_E0.6231.00
191_F195_P0.6221.00
106_T219_M0.6201.00
189_E193_K0.6191.00
119_A199_D0.6181.00
197_G200_Y0.6141.00
143_T215_V0.6101.00
138_V161_S0.6101.00
74_L93_L0.6091.00
40_E91_S0.6081.00
32_T48_L0.6071.00
70_V86_I0.6041.00
237_A240_R0.6021.00
60_C64_N0.5961.00
188_S202_F0.5961.00
192_L195_P0.5941.00
53_I69_F0.5921.00
36_Y74_L0.5911.00
81_L222_R0.5891.00
114_S181_F0.5861.00
81_L103_I0.5831.00
19_A65_T0.5821.00
82_K222_R0.5771.00
93_L103_I0.5771.00
31_G77_L0.5761.00
49_V252_Y0.5741.00
149_N159_I0.5721.00
43_N46_G0.5721.00
59_L219_M0.5691.00
33_D91_S0.5691.00
243_G246_E0.5681.00
16_S20_A0.5661.00
191_F194_Q0.5641.00
52_D92_S0.5621.00
160_V177_I0.5621.00
136_V148_G0.5611.00
148_G152_W0.5601.00
60_C63_I0.5581.00
61_K244_T0.5581.00
195_P199_D0.5571.00
105_F218_G0.5571.00
21_F24_I0.5571.00
108_K111_A0.5571.00
85_K176_R0.5561.00
148_G181_F0.5561.00
60_C65_T0.5531.00
31_G89_I0.5521.00
33_D139_L0.5491.00
107_D236_F0.5491.00
31_G72_N0.5471.00
100_Q104_A0.5411.00
23_A231_A0.5331.00
54_D248_L0.5291.00
36_Y39_F0.5281.00
173_T200_Y0.5251.00
121_N175_G0.5241.00
239_M248_L0.5231.00
132_K152_W0.5231.00
113_D208_K0.5221.00
158_E161_S0.5191.00
110_Y217_T0.5151.00
182_Q186_A0.5131.00
183_D217_T0.5111.00
34_P42_K0.5101.00
25_P87_D0.5081.00
36_Y40_E0.5071.00
56_A60_C0.5061.00
149_N153_A0.5051.00
240_R243_G0.5051.00
116_L131_L0.5051.00
138_V148_G0.5021.00
177_I180_A0.5011.00
77_L93_L0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2ylnA 1 0.8846 100 0.271 Contact Map
1xt8A 1 0.8962 100 0.276 Contact Map
4powA 1 0.8846 100 0.277 Contact Map
2yjpA 1 0.8885 100 0.278 Contact Map
3hv1A 2 0.9423 100 0.281 Contact Map
3kbrA 4 0.8692 100 0.283 Contact Map
3k4uA 5 0.8846 100 0.288 Contact Map
4q0cA 2 0.9654 100 0.29 Contact Map
1pb7A 1 0.9231 100 0.291 Contact Map
2vhaA 2 0.9308 100 0.291 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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