GLNP - ECOLI - GREMLIN Contacts

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GLNP - Glutamine transport system permease protein GlnP

UniProt:	P0AEQ6	Sequences: MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFARTFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSGAYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIKDTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKIL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG10388
Length:	219 (203)
Sequences:	20660 (12597)
Seq/√Len:	884.1

ECOLI - GLNP HISM GLTJ GLTK HISQ ARTQ YECS YHDY ARTM YHDX YEHY METI PHNE CYST CYSW YDCU PROW THIP POTI PSTC POTH PSTA POTC YDCV YCJP POTB YEHW UGPA TAUC YNJC MODB SSUC MALG UGPE YCJO MALF
BACSU - TCYM ARTQ GLNP GLNM YCKA YXEN TCYL TCYB METP YQGH OPUBB OPUCB OPUBD AMYC ARAP YQGI OPUAB OPUCD MDXF YURN AMYD MDXG GANP YTEP SSUC YURM GANQ YVGM YTLD LPLB YESQ YTCP ARAQ LPLC YESP
HALSA - Q9HMT4 Q9HML6 Q9HS11 Q9HP02 Q9HR05 Q9HNI9 Q9HPK3 Q9HP01 Q9HHQ7 Q9HHQ6 Q9HML7 Q9HS12
SULSO - Q97YY3 Q97ZC1 Q97UY9 Q97ZC2 Q97UF3 Q97YY2 Q97UF4 Q97UZ0 Q97ZT6 Q97ZT7 Q97Z98 Q97VC0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
32_G	98_M	3.272	1.00
152_G	209_L	3.193	1.00
124_G	134_T	3.139	1.00
186_R	189_E	3.021	1.00
93_A	165_F	2.869	1.00
20_K	24_W	2.823	1.00
130_S	133_E	2.719	1.00
43_A	55_A	2.540	1.00
177_G	194_V	2.461	1.00
132_W	136_R	2.260	1.00
125_L	134_T	2.254	1.00
65_T	70_Q	2.234	1.00
207_F	211_R	2.184	1.00
40_A	106_A	2.164	1.00
120_F	142_L	2.118	1.00
121_R	134_T	2.117	1.00
50_I	54_V	2.021	1.00
28_L	94_V	1.995	1.00
40_A	102_G	1.973	1.00
121_R	125_L	1.912	1.00
175_R	179_E	1.888	1.00
50_I	53_H	1.866	1.00
49_W	53_H	1.803	1.00
21_M	24_W	1.790	1.00
41_G	109_T	1.790	1.00
36_I	102_G	1.775	1.00
44_R	56_L	1.774	1.00
24_W	90_F	1.691	1.00
203_L	207_F	1.689	1.00
16_I	20_K	1.677	1.00
30_L	155_W	1.646	1.00
45_T	114_L	1.614	1.00
74_I	96_T	1.582	1.00
211_R	214_R	1.578	1.00
207_F	210_R	1.569	1.00
199_L	203_L	1.563	1.00
17_E	20_K	1.555	1.00
19_A	201_I	1.555	1.00
21_M	170_V	1.541	1.00
115_S	146_R	1.539	1.00
133_E	137_Y	1.537	1.00
131_R	135_I	1.519	1.00
25_I	165_F	1.517	1.00
177_G	190_I	1.503	1.00
38_L	109_T	1.500	1.00
130_S	134_T	1.499	1.00
112_A	147_M	1.497	1.00
26_S	159_I	1.472	1.00
107_E	110_R	1.467	1.00
181_I	187_A	1.465	1.00
14_L	17_E	1.454	1.00
59_I	106_A	1.454	1.00
33_G	101_S	1.437	1.00
105_I	151_L	1.408	1.00
125_L	131_R	1.389	1.00
129_L	133_E	1.386	1.00
208_I	211_R	1.363	1.00
176_Q	179_E	1.338	1.00
88_D	91_T	1.337	1.00
133_E	136_R	1.334	1.00
40_A	55_A	1.321	1.00
49_W	52_N	1.316	1.00
70_Q	100_N	1.288	1.00
105_I	154_Q	1.278	1.00
28_L	98_M	1.276	1.00
48_G	53_H	1.259	1.00
175_R	178_Q	1.251	1.00
109_T	147_M	1.247	1.00
26_S	162_T	1.244	1.00
110_R	114_L	1.235	1.00
48_G	51_A	1.223	1.00
24_W	28_L	1.223	1.00
12_I	16_I	1.204	1.00
32_G	94_V	1.203	1.00
124_G	138_V	1.200	1.00
35_V	39_L	1.193	1.00
39_L	43_A	1.184	1.00
121_R	139_I	1.182	1.00
14_L	176_Q	1.155	1.00
53_H	57_V	1.145	1.00
171_A	175_R	1.140	1.00
131_R	134_T	1.133	1.00
90_F	94_V	1.126	1.00
62_I	99_I	1.125	1.00
94_V	98_M	1.119	1.00
37_G	105_I	1.118	1.00
26_S	155_W	1.106	1.00
71_V	96_T	1.096	1.00
54_V	57_V	1.095	1.00
108_I	154_Q	1.095	1.00
71_V	165_F	1.087	1.00
91_T	94_V	1.083	1.00
17_E	21_M	1.071	1.00
52_N	56_L	1.060	1.00
13_P	17_E	1.058	1.00
129_L	137_Y	1.055	1.00
178_Q	181_I	1.054	1.00
70_Q	74_I	1.053	1.00
65_T	100_N	1.053	1.00
113_V	139_I	1.045	1.00
181_I	191_W	1.043	1.00
60_E	203_L	1.036	1.00
210_R	214_R	1.032	1.00
211_R	215_R	1.021	1.00
54_V	58_F	1.020	1.00
179_E	183_G	1.003	1.00
89_P	169_G	0.995	1.00
43_A	46_F	0.980	1.00
159_I	201_I	0.977	1.00
34_L	151_L	0.973	1.00
44_R	107_E	0.963	1.00
189_E	192_S	0.961	1.00
55_A	106_A	0.959	1.00
135_I	140_L	0.958	1.00
108_I	150_P	0.953	1.00
45_T	113_V	0.950	1.00
118_K	122_E	0.941	1.00
90_F	170_V	0.936	1.00
116_I	143_A	0.934	1.00
29_G	98_M	0.930	1.00
67_I	161_D	0.928	1.00
124_G	129_L	0.925	1.00
74_I	92_A	0.921	1.00
157_I	161_D	0.916	1.00
62_I	103_A	0.914	1.00
67_I	100_N	0.904	1.00
30_L	158_S	0.903	1.00
204_V	208_I	0.902	1.00
184_N	187_A	0.902	1.00
43_A	51_A	0.900	1.00
28_L	32_G	0.887	1.00
208_I	212_L	0.887	1.00
29_G	158_S	0.883	1.00
31_A	35_V	0.881	1.00
167_V	171_A	0.880	1.00
41_G	113_V	0.875	1.00
146_R	150_P	0.873	1.00
112_A	146_R	0.867	1.00
135_I	139_I	0.866	1.00
57_V	60_E	0.864	1.00
180_I	190_I	0.860	1.00
210_R	213_E	0.857	1.00
58_F	102_G	0.856	1.00
47_G	52_N	0.854	1.00
73_F	77_A	0.853	1.00
163_S	198_Y	0.847	1.00
165_F	170_V	0.847	1.00
22_T	159_I	0.846	1.00
71_V	164_L	0.840	1.00
72_M	76_F	0.837	1.00
77_A	81_A	0.837	1.00
60_E	199_L	0.835	1.00
42_F	135_I	0.829	1.00
48_G	54_V	0.829	1.00
19_A	197_F	0.826	1.00
70_Q	96_T	0.826	1.00
27_V	31_A	0.822	1.00
101_S	154_Q	0.817	1.00
41_G	45_T	0.817	1.00
53_H	56_L	0.809	1.00
116_I	146_R	0.804	1.00
13_P	16_I	0.799	1.00
91_T	95_V	0.798	1.00
177_G	181_I	0.796	1.00
58_F	62_I	0.795	1.00
165_F	172_E	0.794	1.00
38_L	140_L	0.790	1.00
36_I	98_M	0.790	1.00
48_G	52_N	0.783	1.00
159_I	205_L	0.783	1.00
113_V	143_A	0.777	1.00
46_F	55_A	0.775	1.00
18_G	176_Q	0.773	1.00
56_L	60_E	0.772	1.00
28_L	90_F	0.772	1.00
193_A	197_F	0.769	1.00
118_K	121_R	0.767	1.00
121_R	131_R	0.757	1.00
119_G	123_A	0.757	1.00
71_V	168_I	0.754	1.00
68_V	167_V	0.752	1.00
205_L	209_L	0.747	1.00
171_A	176_Q	0.746	1.00
101_S	158_S	0.746	1.00
33_G	105_I	0.746	1.00
183_G	186_R	0.744	1.00
19_A	173_L	0.740	1.00
97_I	162_T	0.729	1.00
159_I	202_T	0.728	1.00
21_M	90_F	0.727	1.00
105_I	109_T	0.722	1.00
200_I	204_V	0.722	1.00
93_A	168_I	0.719	1.00
58_F	103_A	0.717	1.00
29_G	97_I	0.717	1.00
25_I	29_G	0.716	1.00
89_P	170_V	0.715	1.00
51_A	54_V	0.715	1.00
109_T	113_V	0.712	1.00
73_F	192_S	0.703	1.00
46_F	131_R	0.701	1.00
28_L	31_A	0.694	1.00
75_Y	92_A	0.694	1.00
23_L	27_V	0.689	1.00
25_I	93_A	0.689	1.00
191_W	194_V	0.685	1.00
32_G	35_V	0.682	1.00
44_R	47_G	0.682	1.00
139_I	142_L	0.679	1.00
100_N	161_D	0.678	1.00
75_Y	168_I	0.677	1.00
206_S	210_R	0.677	1.00
25_I	170_V	0.675	1.00
192_S	195_A	0.675	1.00
173_L	176_Q	0.668	1.00
40_A	43_A	0.667	1.00
26_S	30_L	0.665	1.00
192_S	196_V	0.664	1.00
163_S	166_I	0.664	1.00
25_I	90_F	0.661	1.00
150_P	153_N	0.657	1.00
77_A	80_M	0.656	1.00
191_W	195_A	0.655	1.00
89_P	92_A	0.650	1.00
196_V	200_I	0.647	1.00
212_L	216_M	0.647	1.00
194_V	198_Y	0.647	1.00
166_I	175_R	0.644	1.00
44_R	52_N	0.638	1.00
16_I	19_A	0.638	1.00
119_G	122_E	0.637	1.00
181_I	190_I	0.633	1.00
101_S	161_D	0.632	1.00
203_L	206_S	0.630	1.00
97_I	165_F	0.628	1.00
22_T	198_Y	0.624	1.00
43_A	54_V	0.622	1.00
61_V	65_T	0.621	1.00
118_K	125_L	0.619	1.00
97_I	158_S	0.617	1.00
37_G	109_T	0.617	1.00
154_Q	157_I	0.613	1.00
174_T	191_W	0.604	1.00
37_G	41_G	0.597	1.00
36_I	39_L	0.597	1.00
209_L	212_L	0.596	1.00
62_I	70_Q	0.596	1.00
98_M	102_G	0.590	1.00
204_V	211_R	0.589	1.00
44_R	59_I	0.587	1.00
32_G	36_I	0.586	1.00
101_S	105_I	0.585	1.00
155_W	205_L	0.580	1.00
20_K	23_L	0.579	1.00
177_G	180_I	0.577	1.00
24_W	91_T	0.574	1.00
30_L	34_L	0.571	1.00
44_R	106_A	0.569	1.00
36_I	40_A	0.567	1.00
178_Q	182_A	0.567	1.00
75_Y	89_P	0.565	1.00
35_V	98_M	0.564	1.00
185_F	188_L	0.562	1.00
122_E	137_Y	0.558	1.00
204_V	207_F	0.557	1.00
148_L	212_L	0.556	1.00
67_I	97_I	0.556	1.00
75_Y	87_I	0.555	1.00
34_L	109_T	0.554	1.00
117_H	146_R	0.553	1.00
93_A	170_V	0.553	1.00
149_P	213_E	0.550	1.00
180_I	184_N	0.548	1.00
38_L	144_L	0.546	1.00
174_T	194_V	0.545	1.00
130_S	137_Y	0.544	1.00
47_G	131_R	0.544	1.00
148_L	216_M	0.543	1.00
60_E	206_S	0.542	1.00
124_G	127_L	0.542	1.00
194_V	197_F	0.540	1.00
129_L	134_T	0.539	1.00
121_R	135_I	0.538	1.00
188_L	192_S	0.538	1.00
21_M	172_E	0.537	1.00
163_S	174_T	0.535	1.00
89_P	168_I	0.535	1.00
35_V	38_L	0.531	1.00
15_L	197_F	0.525	1.00
172_E	176_Q	0.525	1.00
82_F	85_L	0.522	1.00
113_V	135_I	0.520	1.00
37_G	106_A	0.519	1.00
17_E	24_W	0.519	1.00
47_G	50_I	0.518	1.00
174_T	198_Y	0.517	1.00
112_A	150_P	0.517	1.00
100_N	104_Y	0.514	1.00
207_F	214_R	0.508	1.00
87_I	91_T	0.508	1.00
34_L	105_I	0.508	1.00
64_G	100_N	0.508	1.00
199_L	202_T	0.505	1.00
34_L	155_W	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4ymuD	2	0.9817	100	0.359	Contact Map
2onkC	2	0.9635	99.9	0.426	Contact Map
3rlfF	1	0.9452	99.9	0.434	Contact Map
3d31C	2	0.9452	99.9	0.438	Contact Map
3tuiA	2	0.9543	99.9	0.442	Contact Map
4tquM	1	0.9909	99.9	0.467	Contact Map
3rlfG	1	0.9954	99.9	0.487	Contact Map
4tquN	1	0.9543	99.9	0.519	Contact Map
1hgvA	8	0.21	2.6	0.957	Contact Map
4mt1A	3	0.79	2.1	0.959	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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