GREMLIN Database
FRE - NAD(P)H-flavin reductase
UniProt: P0AEN1 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10334
Length: 233 (215)
Sequences: 20115 (14303)
Seq/√Len: 975.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
105_P133_D3.3141.00
22_R60_F2.8421.00
9_T24_V2.6411.00
124_T128_R2.6111.00
82_L85_H2.6041.00
20_R62_E2.5751.00
135_T165_V2.5721.00
149_L153_E2.5331.00
14_I20_R2.4801.00
11_V19_Y2.4221.00
22_R62_E2.3171.00
123_L151_E2.3101.00
7_K24_V2.2881.00
7_K86_Q2.2791.00
111_G203_R2.2551.00
110_A118_A2.2301.00
13_A19_Y2.1321.00
38_M48_P2.1231.00
10_S22_R2.1211.00
40_V88_V2.0991.00
107_I198_I2.0981.00
135_T163_Q2.0791.00
8_V81_I2.0421.00
37_L63_L2.0031.00
52_A64_H2.0021.00
12_E20_R1.9771.00
146_L168_V1.9521.00
198_I214_F1.9141.00
115_F136_I1.7981.00
110_A138_W1.7941.00
133_D163_Q1.7711.00
143_E171_P1.6961.00
140_G146_L1.6651.00
184_T187_L1.6331.00
10_S83_K1.6251.00
21_V77_V1.6231.00
154_A158_K1.6221.00
142_E145_H1.5961.00
198_I220_A1.5801.00
105_P135_T1.5241.00
24_V60_F1.5181.00
139_G206_M1.5131.00
109_I206_M1.4991.00
211_R215_C1.4981.00
23_I63_L1.4961.00
184_T188_Q1.4791.00
35_Q94_G1.4751.00
37_L89_V1.4741.00
200_I210_A1.4561.00
105_P196_H1.4471.00
104_R197_D1.4471.00
9_T60_F1.4411.00
104_R195_E1.4291.00
67_A75_K1.4261.00
111_G206_M1.4041.00
16_D144_Q1.3931.00
67_A78_M1.3861.00
109_I210_A1.3801.00
139_G182_V1.3771.00
139_G169_E1.3691.00
108_L118_A1.3551.00
12_E22_R1.3231.00
215_C222_E1.3131.00
119_R123_L1.3021.00
18_V113_T1.2821.00
8_V23_I1.2791.00
37_L51_M1.2581.00
204_F208_K1.2431.00
152_L164_V1.2261.00
119_R151_E1.2261.00
165_V178_R1.2111.00
153_E156_S1.2081.00
185_A188_Q1.2021.00
38_M90_D1.1991.00
64_H147_Y1.1971.00
143_E149_L1.1951.00
64_H148_D1.1921.00
11_V21_V1.1871.00
113_T116_S1.1731.00
212_D217_E1.1691.00
126_L159_H1.1671.00
200_I207_A1.1651.00
118_A136_I1.1631.00
20_R64_H1.1611.00
19_Y78_M1.1491.00
110_A136_I1.1461.00
119_R148_D1.1451.00
140_G166_P1.1441.00
185_A189_D1.1411.00
169_E181_T1.1271.00
20_R148_D1.1241.00
180_G183_L1.1181.00
136_I162_L1.0961.00
107_I196_H1.0771.00
11_V83_K1.0741.00
146_L166_P1.0691.00
213_L217_E1.0651.00
17_T145_H1.0621.00
7_K26_D1.0591.00
153_E166_P1.0561.00
8_V21_V1.0481.00
126_L158_K1.0481.00
125_A134_I1.0431.00
54_T123_L1.0421.00
108_L121_I1.0391.00
122_L134_I1.0371.00
143_E168_V1.0371.00
197_D224_R1.0351.00
141_R169_E1.0331.00
108_L134_I1.0331.00
71_N74_A1.0271.00
205_E209_I1.0221.00
41_M73_Y1.0191.00
40_V46_K1.0151.00
26_D86_Q1.0081.00
178_R189_D1.0061.00
159_H162_L0.9971.00
151_E154_A0.9961.00
17_T66_G0.9951.00
81_I85_H0.9951.00
178_R181_T0.9811.00
137_Y165_V0.9751.00
11_V82_L0.9751.00
149_L164_V0.9711.00
56_D127_A0.9691.00
146_L149_L0.9651.00
142_E170_Q0.9611.00
153_E157_L0.9601.00
184_T189_D0.9601.00
208_K212_D0.9511.00
18_V147_Y0.9481.00
39_V63_L0.9441.00
209_I212_D0.9431.00
76_A80_R0.9431.00
140_G168_V0.9281.00
57_E123_L0.9261.00
139_G167_V0.9211.00
32_R97_W0.9211.00
33_A124_T0.9201.00
184_T217_E0.9131.00
154_A157_L0.9101.00
150_C154_A0.9031.00
68_S71_N0.8951.00
49_F74_A0.8931.00
209_I213_L0.8881.00
221_R224_R0.8751.00
15_T145_H0.8691.00
106_M125_A0.8671.00
98_L106_M0.8571.00
28_A61_I0.8541.00
115_F152_L0.8521.00
16_D145_H0.8521.00
98_L121_I0.8491.00
25_P28_A0.8411.00
150_C153_E0.8351.00
188_Q218_R0.8341.00
138_W147_Y0.8331.00
108_L122_L0.8301.00
122_L152_L0.8291.00
184_T213_L0.8271.00
31_F55_P0.8211.00
45_D72_L0.8201.00
8_V11_V0.8201.00
192_T196_H0.8171.00
140_G145_H0.8091.00
212_D215_C0.8091.00
53_S123_L0.8041.00
45_D73_Y0.8011.00
148_D151_E0.7991.00
104_R132_R0.7971.00
126_L155_L0.7911.00
77_V81_I0.7901.00
122_L136_I0.7881.00
69_E72_L0.7861.00
64_H116_S0.7851.00
122_L155_L0.7851.00
213_L218_R0.7841.00
137_Y167_V0.7841.00
65_I77_V0.7811.00
57_E60_F0.7781.00
115_F138_W0.7751.00
149_L166_P0.7731.00
141_R170_Q0.7701.00
181_T205_E0.7701.00
56_D59_G0.7671.00
156_S160_P0.7581.00
31_F120_S0.7511.00
39_V77_V0.7481.00
52_A62_E0.7471.00
24_V59_G0.7461.00
54_T127_A0.7421.00
70_I75_K0.7411.00
109_I137_Y0.7401.00
106_M134_I0.7381.00
175_W179_T0.7361.00
13_A16_D0.7351.00
105_P132_R0.7301.00
46_K90_D0.7261.00
9_T22_R0.7211.00
108_L199_Y0.7201.00
193_L196_H0.7171.00
151_E155_L0.7161.00
111_G205_E0.7161.00
31_F53_S0.7151.00
195_E224_R0.7141.00
104_R224_R0.7121.00
32_R35_Q0.7101.00
90_D93_H0.7051.00
31_F54_T0.7041.00
56_D124_T0.7031.00
178_R188_Q0.7011.00
183_L217_E0.7001.00
50_S118_A0.6991.00
183_L186_V0.6991.00
55_P124_T0.6991.00
73_Y77_V0.6981.00
53_S62_E0.6961.00
200_I214_F0.6961.00
156_S162_L0.6941.00
21_V65_I0.6941.00
200_I211_R0.6911.00
115_F148_D0.6871.00
170_Q181_T0.6851.00
14_I64_H0.6851.00
124_T127_A0.6841.00
106_M132_R0.6781.00
172_E175_W0.6741.00
18_V64_H0.6731.00
109_I198_I0.6721.00
94_G97_W0.6691.00
122_L162_L0.6661.00
43_E76_A0.6651.00
142_E171_P0.6611.00
210_A213_L0.6611.00
38_M46_K0.6601.00
31_F51_M0.6591.00
51_M61_I0.6511.00
36_Y48_P0.6511.00
121_I199_Y0.6481.00
40_V90_D0.6401.00
119_R152_L0.6391.00
91_I94_G0.6371.00
137_Y189_D0.6351.00
76_A81_I0.6341.00
107_I135_T0.6321.00
23_I87_I0.6311.00
47_R73_Y0.6311.00
25_P29_F0.6301.00
37_L61_I0.6281.00
126_L134_I0.6271.00
192_T195_E0.6241.00
47_R72_L0.6231.00
65_I74_A0.6231.00
177_G180_G0.6201.00
57_E127_A0.6151.00
171_P175_W0.6121.00
208_K211_R0.6041.00
187_L218_R0.6031.00
181_T209_I0.6011.00
99_R197_D0.6001.00
100_D124_T0.6001.00
38_M93_H0.5961.00
47_R71_N0.5951.00
156_S164_V0.5901.00
103_E197_D0.5901.00
206_M209_I0.5861.00
220_A224_R0.5841.00
143_E175_W0.5841.00
126_L162_L0.5821.00
74_A78_M0.5801.00
143_E172_E0.5801.00
17_T141_R0.5791.00
96_A117_Y0.5791.00
40_V44_R0.5791.00
123_L155_L0.5731.00
106_M197_D0.5721.00
62_E151_E0.5711.00
182_V186_V0.5691.00
41_M45_D0.5681.00
111_G139_G0.5681.00
43_E72_L0.5671.00
19_Y67_A0.5621.00
98_L125_A0.5591.00
103_E131_N0.5581.00
169_E209_I0.5581.00
171_P179_T0.5551.00
66_G203_R0.5541.00
49_F201_A0.5531.00
187_L217_E0.5511.00
61_I89_V0.5511.00
57_E62_E0.5461.00
156_S163_Q0.5451.00
21_V78_M0.5441.00
103_E106_M0.5421.00
133_D161_G0.5401.00
39_V89_V0.5391.00
133_D194_A0.5371.00
54_T57_E0.5351.00
81_I87_I0.5341.00
53_S57_E0.5331.00
69_E75_K0.5281.00
72_L75_K0.5261.00
17_T67_A0.5261.00
105_P195_E0.5241.00
106_M199_Y0.5231.00
33_A55_P0.5221.00
135_T196_H0.5201.00
38_M47_R0.5191.00
32_R124_T0.5181.00
205_E208_K0.5171.00
148_D152_L0.5141.00
25_P61_I0.5121.00
35_Q97_W0.5111.00
64_H113_T0.5091.00
167_V186_V0.5081.00
100_D128_R0.5061.00
34_G50_S0.5061.00
122_L125_A0.5041.00
203_R207_A0.5021.00
11_V14_I0.5021.00
67_A74_A0.5011.00
111_G209_I0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1qfjA 1 0.9657 100 0.164 Contact Map
1tvcA 1 0.9957 100 0.181 Contact Map
1krhA 1 0.9828 100 0.191 Contact Map
1gvhA 3 0.9914 100 0.192 Contact Map
1cqxA 2 0.9871 100 0.204 Contact Map
4p6vF 1 0.9828 100 0.21 Contact Map
2r6hA 1 0.9785 100 0.214 Contact Map
4g1vA 3 0.9571 100 0.221 Contact Map
4u9uA 1 0.9828 100 0.23 Contact Map
2eixA 2 0.97 100 0.232 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0084 seconds.