GREMLIN Database
COBU - Bifunctional adenosylcobalamin biosynthesis protein CobU
UniProt: P0AE76 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13238
Length: 181 (165)
Sequences: 2461 (1878)
Seq/√Len: 146.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
145_S148_A3.8311.00
18_E50_G3.0111.00
35_I44_I2.7831.00
82_C135_N2.7151.00
27_V52_P2.6531.00
63_H66_E2.6171.00
21_I77_V2.5981.00
83_V162_L2.5261.00
18_E29_Y2.4371.00
6_T134_T2.4181.00
46_H50_G2.3701.00
168_E179_K2.2691.00
17_A133_V2.2641.00
16_H170_W2.2211.00
21_I131_V2.1551.00
28_L58_V2.1191.00
3_I170_W2.0971.00
170_W179_K2.0531.00
10_R43_R2.0511.00
6_T174_S2.0421.00
9_A152_R2.0341.00
45_E49_Q2.0311.00
42_A45_E2.0061.00
26_Q76_E1.9881.00
4_L169_V1.9311.00
64_L80_L1.9301.00
4_L134_T1.8191.00
38_D41_A1.8181.00
139_M171_L1.7801.00
88_T92_F1.7711.00
43_R153_D1.7551.00
80_L132_L1.7281.00
77_V129_K1.6931.00
134_T159_N1.4851.00
29_Y50_G1.4611.00
5_V16_H1.4491.00
3_I20_L1.4491.00
117_Q161_Q1.4461.00
141_I148_A1.4361.00
124_Q165_A1.4361.00
30_I80_L1.4151.00
176_I180_I1.3911.00
123_C130_V1.3821.00
87_V158_V1.3251.00
171_L178_V1.3241.00
146_R150_H1.2921.00
18_E22_G1.2821.00
151_F155_A1.2751.00
16_H20_L1.2751.00
123_C132_L1.2681.00
72_I78_V1.2551.00
115_E118_S1.2501.00
42_A46_H1.2301.00
45_E48_R1.2291.00
126_C130_V1.2171.00
144_E148_A1.2111.00
61_W89_N1.2081.00
84_T155_A1.1991.00
90_L112_I1.1891.00
69_H125_R1.1861.00
21_I27_V1.1831.00
27_V54_H1.1821.00
5_V131_V1.1731.00
92_F147_L1.1521.00
88_T151_F1.1441.00
20_L168_E1.1390.99
173_V180_I1.1180.99
16_H172_V1.1050.99
33_S40_M1.0900.99
20_L131_V1.0640.99
143_P152_R1.0620.99
15_R50_G1.0620.99
15_R19_A1.0610.99
3_I129_K1.0440.99
173_V178_V1.0430.99
117_Q121_A1.0250.99
83_V158_V1.0130.99
123_C166_A1.0100.99
65_D115_E0.9950.99
6_T139_M0.9940.99
34_Q93_D0.9940.99
163_A169_V0.9940.99
79_L133_V0.9930.99
39_E142_V0.9850.98
144_E152_R0.9720.98
28_L76_E0.9700.98
169_V180_I0.9690.98
3_I168_E0.9650.98
169_V176_I0.9630.98
28_L75_N0.9600.98
29_Y79_L0.9580.98
10_R153_D0.9570.98
80_L86_M0.9560.98
69_H118_S0.9460.98
63_H115_E0.9400.98
155_A159_N0.9350.98
68_I126_C0.9310.98
22_G27_V0.9250.98
116_I161_Q0.9150.98
141_I152_R0.9070.97
113_N154_I0.8980.97
161_Q165_A0.8950.97
123_C162_L0.8940.97
50_G79_L0.8910.97
38_D42_A0.8830.97
84_T135_N0.8830.97
154_I158_V0.8820.97
112_I116_I0.8790.97
77_V131_V0.8790.97
90_L115_E0.8730.97
132_L166_A0.8630.97
156_G160_Q0.8620.97
176_I181_K0.8580.96
16_H177_G0.8570.96
60_R67_L0.8480.96
159_N163_A0.8380.96
135_N157_R0.8360.96
27_V77_V0.8290.96
23_D26_Q0.8260.96
82_C85_T0.8210.95
116_I158_V0.8160.95
137_V155_A0.8150.95
35_I41_A0.8130.95
30_I67_L0.8090.95
120_I162_L0.8060.95
141_I144_E0.8060.95
121_A125_R0.8030.95
65_D118_S0.8000.95
141_I145_S0.7970.95
142_V152_R0.7930.94
62_Q93_D0.7930.94
136_E139_M0.7820.94
113_N161_Q0.7790.94
64_L86_M0.7790.94
19_A23_D0.7670.93
39_E149_R0.7660.93
34_Q92_F0.7660.93
34_Q88_T0.7610.93
16_H19_A0.7540.93
150_H154_I0.7380.92
157_R161_Q0.7370.92
116_I154_I0.7350.92
36_L92_F0.7260.91
37_D40_M0.7190.91
122_A126_C0.7170.91
139_M173_V0.7160.91
116_I120_I0.7100.90
161_Q164_A0.7100.90
72_I127_P0.7100.90
120_I165_A0.7030.90
36_L41_A0.7010.90
15_R160_Q0.6990.90
46_H49_Q0.6990.90
41_A45_E0.6850.89
80_L130_V0.6810.89
66_E70_A0.6800.89
87_V155_A0.6780.88
33_S85_T0.6760.88
154_I157_R0.6750.88
28_L78_V0.6740.88
10_R157_R0.6730.88
8_G139_M0.6730.88
26_Q75_N0.6680.88
47_H51_R0.6670.88
76_E128_A0.6660.87
52_P55_W0.6660.87
73_N76_E0.6660.87
46_H157_R0.6580.87
3_I6_T0.6550.87
40_M44_I0.6540.87
62_Q90_L0.6530.86
148_A152_R0.6520.86
5_V20_L0.6510.86
140_G152_R0.6490.86
114_A118_S0.6460.86
94_Y150_H0.6440.86
91_L147_L0.6380.85
111_A114_A0.6370.85
122_A125_R0.6370.85
120_I161_Q0.6350.85
153_D160_Q0.6340.85
34_Q61_W0.6340.85
33_S59_E0.6300.84
75_N127_P0.6250.84
170_W177_G0.6160.83
159_N180_I0.6150.83
139_M174_S0.6150.83
72_I76_E0.6100.83
26_Q56_R0.6090.82
62_Q89_N0.6070.82
22_G54_H0.6030.82
80_L83_V0.6020.82
163_A176_I0.5980.81
40_M84_T0.5980.81
81_E85_T0.5970.81
32_T85_T0.5960.81
87_V154_I0.5930.81
25_S129_K0.5860.80
145_S152_R0.5840.80
20_L23_D0.5830.80
84_T87_V0.5830.80
6_T169_V0.5810.79
72_I128_A0.5800.79
173_V176_I0.5750.79
19_A164_A0.5740.79
4_L163_A0.5690.78
90_L95_G0.5660.78
6_T159_N0.5650.77
5_V170_W0.5620.77
58_V67_L0.5620.77
121_A124_Q0.5620.77
36_L88_T0.5590.77
18_E133_V0.5550.76
83_V132_L0.5530.76
9_A143_P0.5510.76
4_L174_S0.5490.75
34_Q62_Q0.5480.75
60_R66_E0.5470.75
8_G159_N0.5440.75
124_Q167_N0.5440.75
138_G156_G0.5440.75
148_A151_F0.5430.75
149_R153_D0.5370.74
78_V130_V0.5360.74
29_Y55_W0.5330.73
60_R86_M0.5320.73
83_V159_N0.5310.73
65_D119_L0.5300.73
25_S77_V0.5300.73
20_L170_W0.5290.73
138_G159_N0.5260.72
18_E79_L0.5260.72
28_L56_R0.5220.72
138_G152_R0.5210.72
137_V141_I0.5200.71
82_C89_N0.5200.71
3_I131_V0.5190.71
149_R152_R0.5170.71
92_F137_V0.5160.71
94_Y115_E0.5150.71
56_R76_E0.5150.71
140_G153_D0.5140.71
112_I154_I0.5140.71
134_T155_A0.5100.70
121_A165_A0.5040.69
85_T88_T0.5040.69
18_E27_V0.5030.69
85_T135_N0.5000.69
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1c9kA 3 0.9945 100 0.124 Contact Map
3cmuA 1 0.9171 98.6 0.828 Contact Map
3io5A 2 0.779 98.5 0.831 Contact Map
3cmwA 1 0.9337 98.4 0.839 Contact Map
4ppfA 1 0.8398 98.3 0.843 Contact Map
3ldaA 1 0.7901 98.3 0.844 Contact Map
1u94A 3 0.8287 98.1 0.851 Contact Map
3hr8A 1 0.9282 98.1 0.852 Contact Map
1xp8A 3 0.8011 97.8 0.862 Contact Map
2z43A 1 0.7956 97.8 0.862 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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