ASNC - ECOLI - GREMLIN Contacts

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ASNC - Regulatory protein AsnC

UniProt:	P0ACI6	Sequences: MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVEKMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTTGHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG10093
Length:	152 (138)
Sequences:	13596 (8773)
Seq/√Len:	746.8

ECOLI - ASNC LRP YBAO
BACSU - AZLB YWRC LRPC YUGG LRPB YEZC LRPA
HALSA - Q9HNI1 Q9HQ13 Q9HQ33 Q9HQB6 Q9HP41 Q9HQG1 Q9HNW8 Q9HP70
SULSO - Q7LXS6 Q7LX67 Q97W90 Q97XP7 LYSM Q97X78 Q980P3 Q97VN7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
16_A	31_Q	2.943	1.00
47_K	55_A	2.891	1.00
75_I	135_S	2.571	1.00
104_H	137_E	2.520	1.00
97_E	110_K	2.479	1.00
25_Y	43_V	2.258	1.00
114_R	118_A	2.254	1.00
62_K	117_D	2.082	1.00
10_D	42_R	2.071	1.00
16_A	23_T	2.044	1.00
45_K	49_A	2.019	1.00
53_T	60_S	1.989	1.00
77_K	134_Q	1.885	1.00
92_L	126_K	1.866	1.00
69_G	110_K	1.851	1.00
53_T	58_D	1.817	1.00
71_F	99_Y	1.788	1.00
96_T	112_M	1.765	1.00
92_L	122_V	1.711	1.00
9_L	34_V	1.708	1.00
16_A	19_G	1.670	1.00
44_E	48_Q	1.667	1.00
34_V	38_T	1.648	1.00
26_A	30_K	1.633	1.00
73_G	139_L	1.587	1.00
23_T	31_Q	1.554	1.00
88_K	130_I	1.547	1.00
115_S	118_A	1.542	1.00
118_A	121_H	1.542	1.00
27_E	30_K	1.506	1.00
26_A	36_P	1.474	1.00
88_K	91_S	1.470	1.00
34_V	39_I	1.464	1.00
94_E	118_A	1.436	1.00
35_S	38_T	1.435	1.00
85_A	89_L	1.426	1.00
25_Y	40_H	1.415	1.00
13_I	28_L	1.413	1.00
86_L	90_E	1.391	1.00
73_G	105_Y	1.383	1.00
74_I	109_I	1.364	1.00
22_R	55_A	1.353	1.00
32_F	39_I	1.338	1.00
75_I	104_H	1.335	1.00
23_T	28_L	1.294	1.00
83_P	87_A	1.292	1.00
121_H	125_N	1.289	1.00
75_I	134_Q	1.286	1.00
8_N	12_G	1.285	1.00
66_Y	114_R	1.285	1.00
72_I	111_V	1.273	1.00
86_L	143_Q	1.237	1.00
27_E	31_Q	1.236	1.00
120_Q	143_Q	1.225	1.00
17_L	43_V	1.214	1.00
9_L	12_G	1.213	1.00
44_E	47_K	1.187	1.00
19_G	31_Q	1.176	1.00
76_L	85_A	1.172	1.00
20_N	31_Q	1.159	1.00
68_V	116_I	1.152	1.00
24_A	27_E	1.144	1.00
94_E	126_K	1.138	1.00
7_D	42_R	1.138	1.00
16_A	28_L	1.109	1.00
73_G	137_E	1.094	1.00
9_L	32_F	1.088	1.00
54_G	58_D	1.074	1.00
81_D	84_S	1.054	1.00
86_L	107_I	1.041	1.00
12_G	16_A	1.034	1.00
113_C	118_A	1.028	1.00
95_V	111_V	1.021	1.00
25_Y	29_A	1.019	1.00
12_G	15_E	1.014	1.00
15_E	19_G	1.009	1.00
98_A	109_I	1.000	1.00
60_S	63_Q	0.982	1.00
23_T	27_E	0.982	1.00
29_A	39_I	0.981	1.00
84_S	88_K	0.971	1.00
21_A	55_A	0.971	1.00
71_F	108_F	0.964	1.00
69_G	112_M	0.960	1.00
87_A	90_E	0.957	1.00
40_H	44_E	0.944	1.00
113_C	119_L	0.942	1.00
12_G	32_F	0.942	1.00
101_T	108_F	0.929	1.00
99_Y	110_K	0.926	1.00
22_R	47_K	0.914	1.00
36_P	40_H	0.913	1.00
67_D	115_S	0.909	1.00
61_P	112_M	0.906	1.00
29_A	34_V	0.900	1.00
25_Y	36_P	0.899	1.00
71_F	110_K	0.897	1.00
113_C	122_V	0.891	1.00
111_V	122_V	0.889	1.00
6_I	11_R	0.879	1.00
71_F	141_V	0.860	1.00
87_A	91_S	0.858	1.00
99_Y	141_V	0.855	1.00
95_V	98_A	0.851	1.00
26_A	40_H	0.844	1.00
46_M	51_I	0.835	1.00
21_A	27_E	0.822	1.00
95_V	109_I	0.821	1.00
10_D	45_K	0.814	1.00
104_H	135_S	0.799	1.00
11_R	15_E	0.791	1.00
88_K	92_L	0.787	1.00
89_L	98_A	0.775	1.00
99_Y	144_N	0.767	1.00
83_P	86_L	0.763	1.00
21_A	57_I	0.761	1.00
28_L	39_I	0.756	1.00
123_L	138_T	0.751	1.00
13_I	42_R	0.750	1.00
28_L	43_V	0.748	1.00
37_G	41_V	0.745	1.00
82_Y	107_I	0.743	1.00
73_G	108_F	0.738	1.00
6_I	51_I	0.738	1.00
121_H	126_K	0.731	1.00
74_I	85_A	0.730	1.00
73_G	104_H	0.727	1.00
84_S	87_A	0.727	1.00
13_I	39_I	0.724	1.00
66_Y	94_E	0.714	1.00
7_D	10_D	0.714	1.00
16_A	20_N	0.709	1.00
105_Y	137_E	0.707	1.00
20_N	23_T	0.706	1.00
51_I	63_Q	0.697	1.00
86_L	145_P	0.696	1.00
128_Q	136_T	0.693	1.00
100_Y	107_I	0.687	1.00
75_I	105_Y	0.682	1.00
94_E	122_V	0.679	1.00
85_A	107_I	0.679	1.00
117_D	120_Q	0.677	1.00
77_K	132_E	0.669	1.00
13_I	23_T	0.663	1.00
70_C	119_L	0.662	1.00
81_D	129_T	0.659	1.00
66_Y	112_M	0.658	1.00
70_C	140_I	0.656	1.00
116_I	140_I	0.652	1.00
102_T	140_I	0.650	1.00
117_D	121_H	0.649	1.00
41_V	45_K	0.648	1.00
8_N	11_R	0.645	1.00
84_S	132_E	0.641	1.00
69_G	99_Y	0.636	1.00
13_I	16_A	0.635	1.00
72_I	136_T	0.630	1.00
89_L	109_I	0.630	1.00
21_A	54_G	0.625	1.00
41_V	44_E	0.623	1.00
10_D	46_M	0.619	1.00
29_A	36_P	0.614	1.00
14_L	46_M	0.614	1.00
13_I	43_V	0.611	1.00
94_E	114_R	0.607	1.00
69_G	144_N	0.601	1.00
69_G	141_V	0.600	1.00
50_G	53_T	0.598	1.00
123_L	136_T	0.595	1.00
72_I	109_I	0.594	1.00
74_I	133_I	0.593	1.00
92_L	130_I	0.592	1.00
42_R	46_M	0.591	1.00
60_S	64_L	0.588	1.00
25_Y	39_I	0.583	1.00
75_I	137_E	0.578	1.00
73_G	101_T	0.570	1.00
59_V	64_L	0.568	1.00
52_I	55_A	0.568	1.00
37_G	40_H	0.567	1.00
83_P	129_T	0.566	1.00
70_C	138_T	0.565	1.00
118_A	122_V	0.563	1.00
13_I	46_M	0.562	1.00
126_K	130_I	0.561	1.00
116_I	142_L	0.560	1.00
58_D	61_P	0.546	1.00
122_V	127_I	0.544	1.00
81_D	131_D	0.544	1.00
101_T	105_Y	0.543	1.00
122_V	126_K	0.535	1.00
101_T	141_V	0.535	1.00
119_L	140_I	0.531	1.00
100_Y	106_S	0.527	1.00
26_A	29_A	0.525	1.00
100_Y	128_Q	0.524	1.00
76_L	106_S	0.524	1.00
9_L	38_T	0.523	1.00
18_M	56_R	0.521	1.00
45_K	48_Q	0.513	1.00
117_D	143_Q	0.507	1.00
78_S	134_Q	0.506	1.00
15_E	18_M	0.505	1.00
24_A	29_A	0.504	1.00
74_I	136_T	0.502	1.00
104_H	139_L	0.501	1.00
96_T	110_K	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4ch7A	1	0.9408	100	0.153	Contact Map
4un1B	1	0.9737	100	0.187	Contact Map
4pcqA	2	0.9803	100	0.208	Contact Map
2cg4A	6	0.9803	100	0.208	Contact Map
2p5vA	5	1	100	0.21	Contact Map
4un1A	1	0.9342	100	0.217	Contact Map
2e1cA	1	0.9605	100	0.224	Contact Map
2ia0A	2	0.9408	100	0.228	Contact Map
3i4pA	5	0.9605	100	0.249	Contact Map
2dbbA	4	0.9539	100	0.25	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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