GREMLIN Database
ISCU - Iron-sulfur cluster assembly scaffold protein IscU
UniProt: P0ACD4 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13395
Length: 128 (116)
Sequences: 2489 (1196)
Seq/√Len: 111.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
48_N54_E4.8591.00
15_R75_E3.5101.00
16_N78_K2.7421.00
20_F57_R2.6321.00
81_S84_E2.6191.00
80_K84_E2.5771.00
72_L94_A2.5001.00
55_D78_K2.4401.00
31_M42_K2.3501.00
19_S59_K2.2511.00
56_A77_V2.2021.00
16_N74_T2.1731.00
9_D15_R2.1031.00
18_G57_R1.8961.00
46_K55_D1.8801.00
53_I85_A1.8311.00
24_D46_K1.8121.00
46_K54_E1.7951.00
72_L96_E1.6691.00
32_V113_A1.6621.00
52_I81_S1.6541.00
94_A104_I1.6391.00
70_S110_A1.6341.00
77_V114_I1.5911.00
44_Q57_R1.5861.00
29_S44_Q1.5781.00
22_N46_K1.5651.00
58_F74_T1.5501.00
10_H71_S1.5381.00
10_H15_R1.5381.00
19_S44_Q1.5351.00
73_V114_I1.5191.00
71_S74_T1.4541.00
15_R71_S1.3811.00
16_N71_S1.3661.00
56_A74_T1.3651.00
28_G117_A1.3050.99
51_G83_D1.2950.99
82_L118_I1.2940.99
47_V82_L1.2880.99
84_E87_A1.2860.99
20_F44_Q1.2850.99
7_V65_S1.2560.99
31_M40_V1.2530.99
24_D28_G1.2350.99
4_S8_I1.2030.99
72_L76_W1.1890.99
86_Q115_K1.1440.99
86_Q122_K1.0970.98
40_V61_Y1.0900.98
17_V67_I1.0390.97
114_I118_I0.9760.96
48_N51_G0.9650.96
115_K119_A0.9520.96
43_L73_V0.9450.95
29_S42_K0.9390.95
69_S111_E0.9180.95
86_Q119_A0.9140.94
119_A122_K0.9070.94
97_L100_P0.9030.94
73_V111_E0.9010.94
10_H75_E0.9010.94
83_D86_Q0.9000.94
48_N52_I0.8950.94
30_G113_A0.8830.93
19_S57_R0.8790.93
82_L86_Q0.8700.93
20_F24_D0.8580.92
6_K93_I0.8560.92
108_I112_D0.8550.92
17_V60_T0.8460.92
34_A101_P0.8450.92
105_H109_L0.8440.92
28_G120_D0.8430.92
16_N75_E0.8340.91
45_I56_A0.8220.91
28_G45_I0.8160.90
15_R74_T0.8140.90
104_I108_I0.7960.89
41_M60_T0.7860.89
17_V59_K0.7860.89
76_W80_K0.7730.88
53_I114_I0.7730.88
47_V51_G0.7680.87
65_S69_S0.7640.87
30_G43_L0.7590.87
72_L93_I0.7580.87
10_H14_P0.7560.87
14_P71_S0.7530.86
37_C105_H0.7520.86
83_D122_K0.7460.86
58_F66_A0.7440.86
69_S110_A0.7390.85
24_D47_V0.7310.85
33_G40_V0.7310.85
32_V116_A0.7290.85
65_S107_S0.7240.84
34_A60_T0.7210.84
99_L115_K0.7090.83
34_A65_S0.7060.83
76_W88_I0.7030.82
77_V85_A0.7030.82
62_G66_A0.7000.82
74_T78_K0.6880.81
96_E100_P0.6870.81
19_S29_S0.6810.80
20_F46_K0.6810.80
95_E98_E0.6800.80
72_L91_T0.6790.80
8_I12_E0.6750.80
66_A103_K0.6630.79
86_Q95_E0.6600.78
44_Q59_K0.6580.78
37_C109_L0.6470.77
89_K115_K0.6390.76
53_I77_V0.6390.76
65_S103_K0.6380.76
18_G55_D0.6360.76
96_E99_L0.6290.75
85_A114_I0.6230.74
101_P105_H0.6220.74
6_K9_D0.6200.74
60_T66_A0.6180.74
116_A119_A0.6180.74
73_V77_V0.6160.73
3_Y38_G0.6130.73
42_K59_K0.6120.73
104_I109_L0.6080.72
4_S12_E0.6010.72
24_D49_D0.5990.71
49_D83_D0.5890.70
23_N81_S0.5800.69
20_F58_F0.5790.69
12_E64_G0.5770.69
47_V117_A0.5740.68
4_S98_E0.5740.68
32_V64_G0.5720.68
38_G62_G0.5720.68
51_G82_L0.5710.68
9_D13_N0.5670.67
51_G54_E0.5640.67
64_G104_I0.5640.67
33_G67_I0.5610.66
18_G59_K0.5600.66
108_I111_E0.5590.66
86_Q118_I0.5590.66
91_T111_E0.5580.66
81_S120_D0.5570.66
7_V68_A0.5560.66
49_D52_I0.5470.64
69_S94_A0.5460.64
47_V54_E0.5460.64
35_P38_G0.5450.64
21_D49_D0.5400.63
91_T98_E0.5370.63
83_D87_A0.5350.63
6_K96_E0.5320.62
4_S100_P0.5310.62
48_N80_K0.5280.62
60_T103_K0.5270.62
34_A111_E0.5270.62
47_V87_A0.5260.61
21_D87_A0.5250.61
30_G120_D0.5240.61
55_D58_F0.5210.61
22_N44_Q0.5200.61
73_V96_E0.5200.61
118_I121_Y0.5190.60
10_H74_T0.5130.60
4_S104_I0.5080.59
94_A111_E0.5080.59
111_E114_I0.5040.58
40_V43_L0.5030.58
60_T105_H0.5000.58
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3lvlA 1 0.9766 100 0.118 Contact Map
2z7eA 5 0.9766 100 0.148 Contact Map
1su0B 1 0.8984 100 0.169 Contact Map
4eb5C 1 0.9531 100 0.173 Contact Map
1xjsA 1 0.9375 100 0.174 Contact Map
2qq4A 5 0.8984 100 0.197 Contact Map
3g0mA 1 0.9297 96.3 0.857 Contact Map
2e5aA 1 0.7891 50.6 0.922 Contact Map
1vqzA 1 0.7734 49.7 0.922 Contact Map
4ce4W 1 0.5 37.9 0.927 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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