HEM2 - ECOLI - GREMLIN Contacts

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HEM2 - Delta-aminolevulinic acid dehydratase

UniProt:	P0ACB2	Sequences: MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDYKAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARMSRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGADFIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKSYQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEYAMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG10428
Length:	324 (315)
Sequences:	2875 (1499)
Seq/√Len:	84.5

ECOLI - HEM2
BACSU - HEM2
HALSA - Q9HMZ1
SULSO - Q980U6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
233_S	259_L	3.562	1.00
226_R	258_E	3.537	1.00
257_R	261_E	3.433	1.00
91_D	94_R	3.424	1.00
87_E	126_S	3.100	1.00
30_D	302_K	3.032	1.00
64_E	315_L	3.029	1.00
117_S	154_A	2.815	1.00
291_E	320_K	2.663	1.00
249_A	301_I	2.651	1.00
166_A	171_Q	2.648	1.00
237_E	265_L	2.570	1.00
100_A	153_V	2.520	1.00
103_S	115_V	2.507	1.00
298_L	317_L	2.486	1.00
233_S	244_L	2.465	1.00
156_A	184_F	2.324	1.00
152_V	178_A	2.265	1.00
107_K	160_D	2.259	1.00
280_K	290_E	2.211	1.00
22_E	222_N	2.202	1.00
139_D	142_A	2.148	1.00
226_R	255_I	2.128	1.00
44_Y	54_M	2.118	1.00
256_V	269_A	2.065	1.00
46_A	54_M	2.024	1.00
260_R	307_D	2.023	1.00
93_W	146_N	2.020	1.00
194_T	233_S	2.019	1.00
66_E	70_N	1.993	1.00
67_R	319_E	1.973	1.00
289_D	292_K	1.969	1.00
281_F	287_A	1.967	1.00
8_P	235_L	1.959	1.00
268_G	308_L	1.906	1.00
95_E	156_A	1.901	1.00
45_K	55_R	1.887	1.00
243_C	268_G	1.878	1.00
15_P	19_A	1.871	1.00
294_V	320_K	1.847	1.00
86_D	90_S	1.834	1.00
103_S	117_S	1.824	1.00
148_G	178_A	1.794	1.00
62_A	110_V	1.794	1.00
69_A	110_V	1.752	1.00
32_V	308_L	1.750	1.00
134_C	137_G	1.741	1.00
227_R	260_R	1.719	1.00
234_L	238_A	1.714	1.00
60_H	63_R	1.700	1.00
295_L	317_L	1.692	1.00
44_Y	60_H	1.664	1.00
93_W	149_K	1.664	1.00
258_E	261_E	1.656	1.00
62_A	109_T	1.630	1.00
47_V	53_V	1.623	1.00
48_E	206_E	1.623	1.00
19_A	74_R	1.619	1.00
6_Q	231_R	1.601	1.00
58_E	105_I	1.593	1.00
250_G	271_Q	1.573	1.00
75_S	116_M	1.567	1.00
132_V	146_N	1.553	1.00
200_F	297_S	1.549	1.00
61_L	106_C	1.534	1.00
66_E	110_V	1.533	1.00
63_R	66_E	1.531	1.00
44_Y	56_I	1.526	1.00
251_A	300_S	1.500	0.99
227_R	258_E	1.494	0.99
294_V	313_F	1.474	0.99
234_L	263_T	1.472	0.99
19_A	29_N	1.471	0.99
43_D	57_P	1.452	0.99
161_F	243_C	1.446	0.99
76_V	113_M	1.434	0.99
25_T	29_N	1.433	0.99
76_V	115_V	1.431	0.99
156_A	182_A	1.417	0.99
253_L	301_I	1.416	0.99
39_E	101_R	1.415	0.99
249_A	269_A	1.412	0.99
121_F	150_Q	1.409	0.99
290_E	294_V	1.398	0.99
137_G	186_D	1.398	0.99
33_L	68_I	1.396	0.99
49_A	206_E	1.389	0.99
67_R	315_L	1.384	0.99
77_M	310_F	1.380	0.99
271_Q	313_F	1.371	0.99
316_D	319_E	1.370	0.99
42_D	60_H	1.368	0.99
134_C	139_D	1.363	0.99
190_M	243_C	1.360	0.99
77_M	163_A	1.354	0.99
259_L	267_I	1.352	0.99
31_L	317_L	1.337	0.99
65_I	110_V	1.327	0.99
38_E	45_K	1.327	0.99
290_E	316_D	1.324	0.99
117_S	159_A	1.318	0.99
279_I	297_S	1.317	0.99
11_L	21_F	1.314	0.99
237_E	244_L	1.314	0.99
119_T	162_I	1.303	0.98
62_A	105_I	1.301	0.98
152_V	182_A	1.300	0.98
65_I	106_C	1.269	0.98
24_T	301_I	1.259	0.98
271_Q	297_S	1.249	0.98
161_F	188_A	1.247	0.98
200_F	251_A	1.232	0.98
65_I	113_M	1.231	0.98
193_S	233_S	1.231	0.98
97_G	100_A	1.228	0.98
64_E	68_I	1.224	0.98
114_I	161_F	1.223	0.98
40_E	59_K	1.222	0.98
288_I	293_V	1.221	0.98
280_K	316_D	1.221	0.98
32_V	116_M	1.214	0.98
148_G	174_A	1.207	0.97
237_E	263_T	1.196	0.97
199_S	292_K	1.193	0.97
22_E	224_M	1.189	0.97
69_A	113_M	1.187	0.97
227_R	307_D	1.186	0.97
56_I	60_H	1.182	0.97
234_L	262_R	1.173	0.97
66_E	109_T	1.167	0.97
65_I	76_V	1.165	0.97
269_A	301_I	1.150	0.97
178_A	182_A	1.142	0.96
251_A	297_S	1.131	0.96
33_L	53_V	1.125	0.96
250_G	297_S	1.124	0.96
283_A	288_I	1.118	0.96
244_L	265_L	1.112	0.96
250_G	300_S	1.102	0.96
200_F	279_I	1.100	0.96
298_L	301_I	1.096	0.96
199_S	293_V	1.087	0.95
89_G	125_T	1.083	0.95
105_I	108_Q	1.083	0.95
134_C	142_A	1.076	0.95
291_E	294_V	1.070	0.95
68_I	315_L	1.069	0.95
120_C	130_C	1.067	0.95
283_A	294_V	1.063	0.95
196_F	221_M	1.062	0.95
244_L	259_L	1.057	0.95
311_S	314_A	1.056	0.95
226_R	257_R	1.056	0.95
85_T	211_A	1.055	0.95
16_A	114_I	1.050	0.94
96_D	101_R	1.043	0.94
13_K	186_D	1.039	0.94
127_H	133_L	1.021	0.94
38_E	43_D	1.015	0.93
62_A	66_E	1.009	0.93
73_I	318_A	1.009	0.93
14_S	188_A	1.004	0.93
116_M	163_A	0.999	0.93
17_L	114_I	0.992	0.93
55_R	209_G	0.989	0.92
37_V	61_L	0.988	0.92
31_L	311_S	0.983	0.92
302_K	309_I	0.968	0.92
224_M	299_G	0.968	0.92
106_C	110_V	0.963	0.91
50_M	277_A	0.960	0.91
107_K	115_V	0.960	0.91
151_A	162_I	0.954	0.91
33_L	315_L	0.952	0.91
38_E	57_P	0.945	0.91
244_L	267_I	0.944	0.91
119_T	154_A	0.943	0.91
297_S	300_S	0.943	0.91
147_L	166_A	0.942	0.90
260_R	264_E	0.939	0.90
279_I	313_F	0.936	0.90
38_E	55_R	0.934	0.90
230_I	233_S	0.933	0.90
297_S	313_F	0.933	0.90
49_A	287_A	0.932	0.90
71_A	315_L	0.931	0.90
13_K	17_L	0.928	0.90
41_I	57_P	0.926	0.90
120_C	216_R	0.926	0.90
86_D	89_G	0.926	0.90
130_C	216_R	0.924	0.90
99_V	124_Y	0.921	0.89
28_L	318_A	0.919	0.89
280_K	284_L	0.919	0.89
17_L	188_A	0.918	0.89
253_L	256_V	0.915	0.89
291_E	295_L	0.914	0.89
58_E	102_M	0.908	0.89
279_I	288_I	0.907	0.89
116_M	161_F	0.901	0.88
69_A	112_E	0.898	0.88
68_I	76_V	0.897	0.88
103_S	159_A	0.894	0.88
93_W	153_V	0.893	0.88
256_V	301_I	0.892	0.88
64_E	67_R	0.890	0.88
229_A	255_I	0.888	0.88
243_C	308_L	0.886	0.88
295_L	320_K	0.884	0.87
173_Q	177_Q	0.882	0.87
123_E	209_G	0.881	0.87
71_A	319_E	0.877	0.87
234_L	264_E	0.877	0.87
279_I	293_V	0.876	0.87
106_C	113_M	0.872	0.87
172_V	189_I	0.867	0.86
317_L	320_K	0.866	0.86
105_I	109_T	0.863	0.86
194_T	244_L	0.861	0.86
200_F	293_V	0.861	0.86
164_P	175_I	0.857	0.86
121_F	146_N	0.856	0.86
35_I	61_L	0.854	0.86
146_N	150_Q	0.847	0.85
253_L	300_S	0.838	0.84
26_L	317_L	0.838	0.84
6_Q	238_A	0.837	0.84
190_M	308_L	0.832	0.84
146_N	149_K	0.831	0.84
40_E	43_D	0.831	0.84
88_T	93_W	0.831	0.84
256_V	306_A	0.830	0.84
154_A	162_I	0.827	0.84
32_V	310_F	0.822	0.83
290_E	320_K	0.821	0.83
39_E	83_H	0.820	0.83
42_D	59_K	0.820	0.83
28_L	31_L	0.819	0.83
103_S	154_A	0.818	0.83
230_I	259_L	0.814	0.83
283_A	290_E	0.807	0.82
208_A	212_L	0.807	0.82
144_L	174_A	0.803	0.82
280_K	294_V	0.796	0.81
171_Q	191_S	0.794	0.81
280_K	313_F	0.793	0.81
279_I	283_A	0.790	0.81
65_I	105_I	0.789	0.81
151_A	175_I	0.784	0.80
271_Q	279_I	0.782	0.80
281_F	285_A	0.780	0.80
106_C	115_V	0.779	0.80
80_G	130_C	0.778	0.80
66_E	69_A	0.774	0.79
119_T	166_A	0.772	0.79
313_F	320_K	0.772	0.79
314_A	318_A	0.771	0.79
14_S	17_L	0.771	0.79
58_E	98_L	0.770	0.79
26_L	31_L	0.768	0.79
145_E	149_K	0.767	0.79
133_L	138_V	0.762	0.78
80_G	120_C	0.761	0.78
43_D	60_H	0.759	0.78
104_R	156_A	0.758	0.78
200_F	250_G	0.755	0.78
295_L	299_G	0.754	0.78
264_E	267_I	0.746	0.77
80_G	216_R	0.743	0.77
244_L	263_T	0.741	0.76
120_C	123_E	0.740	0.76
122_C	130_C	0.739	0.76
245_M	310_F	0.738	0.76
194_T	259_L	0.734	0.76
20_M	74_R	0.732	0.76
26_L	295_L	0.731	0.75
230_I	234_L	0.730	0.75
35_I	78_T	0.730	0.75
87_E	127_H	0.729	0.75
53_V	276_Y	0.729	0.75
104_R	108_Q	0.728	0.75
35_I	64_E	0.726	0.75
123_E	130_C	0.724	0.75
81_I	123_E	0.723	0.75
56_I	64_E	0.721	0.74
125_T	133_L	0.720	0.74
61_L	65_I	0.717	0.74
14_S	114_I	0.716	0.74
229_A	233_S	0.716	0.74
200_F	313_F	0.714	0.74
225_N	228_E	0.703	0.73
38_E	41_I	0.703	0.73
119_T	171_Q	0.700	0.72
6_Q	235_L	0.699	0.72
47_V	206_E	0.694	0.72
76_V	106_C	0.692	0.71
58_E	62_A	0.692	0.71
251_A	301_I	0.689	0.71
43_D	59_K	0.688	0.71
26_L	298_L	0.688	0.71
147_L	171_Q	0.684	0.71
45_K	209_G	0.681	0.70
133_L	137_G	0.680	0.70
13_K	137_G	0.678	0.70
278_M	282_A	0.676	0.70
191_S	194_T	0.675	0.70
8_P	11_L	0.671	0.69
55_R	123_E	0.671	0.69
81_I	209_G	0.669	0.69
41_I	59_K	0.668	0.69
290_E	313_F	0.664	0.68
123_E	216_R	0.661	0.68
230_I	262_R	0.660	0.68
251_A	271_Q	0.658	0.68
122_C	208_A	0.658	0.68
152_V	155_A	0.656	0.67
138_V	168_M	0.655	0.67
245_M	268_G	0.654	0.67
133_L	136_H	0.649	0.67
283_A	289_D	0.648	0.66
37_V	102_M	0.647	0.66
78_T	103_S	0.646	0.66
223_P	299_G	0.643	0.66
268_G	310_F	0.643	0.66
250_G	301_I	0.642	0.66
65_I	68_I	0.641	0.66
36_F	204_F	0.640	0.65
293_V	297_S	0.639	0.65
57_P	60_H	0.635	0.65
21_F	222_N	0.633	0.65
19_A	224_M	0.632	0.64
271_Q	298_L	0.627	0.64
122_C	216_R	0.627	0.64
155_A	187_T	0.625	0.64
51_P	287_A	0.624	0.64
69_A	111_P	0.621	0.63
44_Y	64_E	0.620	0.63
62_A	65_I	0.619	0.63
130_C	208_A	0.618	0.63
58_E	101_R	0.617	0.63
294_V	316_D	0.617	0.63
143_T	147_L	0.615	0.62
260_R	265_L	0.614	0.62
107_K	111_P	0.613	0.62
227_R	257_R	0.613	0.62
100_A	150_Q	0.611	0.62
77_M	116_M	0.609	0.62
71_A	318_A	0.607	0.61
280_K	283_A	0.606	0.61
110_V	113_M	0.605	0.61
100_A	154_A	0.604	0.61
133_L	139_D	0.604	0.61
155_A	188_A	0.604	0.61
172_V	175_I	0.603	0.61
263_T	267_I	0.602	0.61
178_A	181_A	0.602	0.61
77_M	80_G	0.600	0.60
120_C	208_A	0.598	0.60
73_I	315_L	0.598	0.60
35_I	76_V	0.598	0.60
227_R	264_E	0.597	0.60
15_P	22_E	0.597	0.60
11_L	188_A	0.596	0.60
233_S	237_E	0.595	0.60
121_F	132_V	0.593	0.60
121_F	171_Q	0.591	0.59
40_E	57_P	0.591	0.59
61_L	102_M	0.589	0.59
41_I	101_R	0.588	0.59
197_A	217_K	0.586	0.59
132_V	150_Q	0.586	0.59
8_P	231_R	0.585	0.59
71_A	74_R	0.583	0.58
294_V	297_S	0.583	0.58
80_G	123_E	0.582	0.58
17_L	20_M	0.581	0.58
193_S	259_L	0.581	0.58
121_F	166_A	0.580	0.58
142_A	145_E	0.579	0.58
84_H	91_D	0.579	0.58
49_A	277_A	0.578	0.58
81_I	85_T	0.576	0.57
93_W	150_Q	0.576	0.57
31_L	116_M	0.575	0.57
23_E	225_N	0.574	0.57
217_K	292_K	0.574	0.57
223_P	252_Y	0.573	0.57
208_A	216_R	0.573	0.57
65_I	109_T	0.573	0.57
16_A	19_A	0.571	0.57
56_I	65_I	0.571	0.57
249_A	256_V	0.571	0.57
260_R	263_T	0.569	0.56
162_I	175_I	0.568	0.56
45_K	123_E	0.567	0.56
258_E	262_R	0.566	0.56
68_I	113_M	0.565	0.56
17_L	161_F	0.565	0.56
25_T	30_D	0.565	0.56
180_D	185_K	0.565	0.56
24_T	299_G	0.563	0.56
52_G	316_D	0.562	0.56
311_S	317_L	0.561	0.55
7_R	10_R	0.559	0.55
42_D	57_P	0.559	0.55
196_F	226_R	0.557	0.55
260_R	267_I	0.556	0.55
36_F	270_Y	0.556	0.55
24_T	224_M	0.555	0.55
38_E	59_K	0.554	0.55
161_F	190_M	0.554	0.55
138_V	219_Y	0.553	0.54
89_G	121_F	0.547	0.54
162_I	189_I	0.545	0.53
176_R	180_D	0.544	0.53
32_V	75_S	0.542	0.53
226_R	261_E	0.541	0.53
30_D	309_I	0.539	0.53
297_S	301_I	0.538	0.52
78_T	99_V	0.535	0.52
92_A	150_Q	0.534	0.52
77_M	122_C	0.534	0.52
50_M	287_A	0.533	0.52
142_A	146_N	0.533	0.52
91_D	150_Q	0.531	0.51
81_I	120_C	0.529	0.51
67_R	71_A	0.529	0.51
66_E	108_Q	0.529	0.51
301_I	306_A	0.528	0.51
313_F	316_D	0.528	0.51
193_S	245_M	0.527	0.51
81_I	216_R	0.524	0.51
232_E	235_L	0.524	0.51
138_V	218_S	0.522	0.50
192_Y	210_S	0.522	0.50
81_I	130_C	0.521	0.50
63_R	67_R	0.521	0.50
143_T	166_A	0.521	0.50
295_L	298_L	0.520	0.50
91_D	123_E	0.520	0.50
24_T	226_R	0.518	0.50
63_R	109_T	0.518	0.50
80_G	122_C	0.518	0.50
174_A	178_A	0.518	0.50
47_V	55_R	0.517	0.50
96_D	104_R	0.517	0.50
204_F	270_Y	0.516	0.49
41_I	44_Y	0.515	0.49
279_I	290_E	0.514	0.49
227_R	231_R	0.514	0.49
35_I	65_I	0.513	0.49
38_E	42_D	0.512	0.49
132_V	142_A	0.512	0.49
277_A	281_F	0.511	0.49
20_M	308_L	0.511	0.49
34_P	204_F	0.510	0.49
154_A	159_A	0.509	0.49
33_L	64_E	0.509	0.49
144_L	148_G	0.508	0.48
257_R	260_R	0.507	0.48
80_G	98_L	0.506	0.48
46_A	284_L	0.504	0.48
73_I	113_M	0.504	0.48
77_M	216_R	0.503	0.48
87_E	132_V	0.503	0.48
234_L	237_E	0.502	0.48
289_D	294_V	0.502	0.48
282_A	287_A	0.502	0.48
259_L	262_R	0.501	0.47
231_R	263_T	0.501	0.47
100_A	212_L	0.500	0.47
66_E	156_A	0.500	0.47

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1l6sA	5	0.9969	100	-0.002	Contact Map
1w1zA	5	0.9815	100	0.006	Contact Map
3obkA	5	0.9969	100	0.012	Contact Map
1w5qA	5	0.9722	100	0.015	Contact Map
1h7nA	5	0.9969	100	0.045	Contact Map
1pv8A	5	0.8673	100	0.054	Contact Map
3vcrA	3	0.6019	96.5	0.953	Contact Map
1wbhA	3	0.5864	96.1	0.955	Contact Map
1vhcA	3	0.5833	94.9	0.959	Contact Map
3i4eA	4	0.8704	94.4	0.96	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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