GREMLIN Database
ASPA - Aspartate ammonia-lyase
UniProt: P0AC38 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10095
Length: 478 (406)
Sequences: 10906 (5656)
Seq/√Len: 280.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
63_K73_A3.9511.00
72_V137_H3.5501.00
56_K81_D3.3921.00
64_E252_K3.3431.00
60_M77_I3.2791.00
400_V403_G3.2681.00
161_I222_A3.1441.00
50_G158_S2.9111.00
60_M256_V2.8361.00
168_N215_V2.8201.00
288_A343_V2.7821.00
171_R212_K2.7471.00
173_G392_N2.6761.00
402_E406_Y2.6581.00
160_L280_V2.6501.00
449_T452_E2.6411.00
117_I121_L2.6311.00
46_E377_H2.6041.00
164_V219_Q2.5991.00
346_N375_S2.5371.00
224_L279_M2.5101.00
200_L396_A2.4941.00
252_K255_E2.4461.00
164_V215_V2.4361.00
177_K180_E2.3601.00
339_V382_A2.3371.00
69_P72_V2.3361.00
285_K347_D2.3301.00
157_Y226_L2.2731.00
63_K70_K2.2361.00
183_D398_K2.2331.00
226_L260_P2.2261.00
168_N219_Q2.0891.00
403_G407_N2.0591.00
313_N395_T2.0581.00
342_K371_A2.0321.00
166_A384_Y2.0121.00
171_R215_V2.0021.00
176_R392_N1.9701.00
173_G387_L1.9591.00
70_K74_N1.9451.00
400_V404_Y1.9411.00
252_K256_V1.9361.00
220_R224_L1.9281.00
173_G391_I1.9201.00
436_K444_E1.9121.00
176_R180_E1.9051.00
215_V219_Q1.8821.00
295_C336_V1.8681.00
289_V344_I1.8601.00
74_N78_A1.8211.00
217_N279_M1.8191.00
53_M158_S1.8061.00
167_I287_L1.7951.00
264_A270_A1.7761.00
228_V254_A1.7611.00
82_E121_L1.7571.00
56_K77_I1.7541.00
220_R223_E1.7501.00
291_M336_V1.7381.00
175_E212_K1.7381.00
439_R454_D1.7251.00
387_L392_N1.7251.00
181_F184_I1.7211.00
402_E460_Q1.7041.00
450_E454_D1.7001.00
423_N426_I1.6991.00
458_S461_N1.6771.00
60_M252_K1.6561.00
171_R208_S1.6551.00
278_V354_A1.6391.00
214_E290_K1.6361.00
169_Q387_L1.6361.00
73_A77_I1.6351.00
398_K402_E1.6331.00
251_K255_E1.6231.00
451_A454_D1.6111.00
213_E269_E1.6111.00
452_E455_D1.6001.00
230_L249_A1.5991.00
227_E262_V1.5861.00
436_K441_V1.5691.00
443_L450_E1.5391.00
349_T367_V1.5261.00
166_A380_T1.5211.00
446_G449_T1.4921.00
61_A252_K1.4861.00
177_K181_F1.4841.00
232_A270_A1.4811.00
75_A122_M1.4711.00
178_A208_S1.4621.00
221_T283_A1.4501.00
285_K351_T1.4421.00
117_I120_E1.4271.00
348_T352_M1.4191.00
223_E260_P1.4041.00
213_E275_G1.4011.00
110_T114_L1.4011.00
159_S380_T1.3841.00
430_I445_R1.3821.00
333_P390_C1.3771.00
397_N400_V1.3701.00
274_C365_E1.3671.00
56_K84_L1.3621.00
94_P119_L1.3621.00
430_I434_T1.3591.00
49_R53_M1.3421.00
216_K224_L1.3311.00
437_S440_E1.3201.00
46_E159_S1.3181.00
282_G286_R1.3151.00
300_L358_Q1.3121.00
156_V280_V1.3121.00
56_K60_M1.3081.00
171_R211_L1.3081.00
423_N447_L1.3031.00
436_K440_E1.3011.00
164_V222_A1.3001.00
289_V354_A1.2981.00
399_E403_G1.2961.00
281_H350_V1.2941.00
175_E179_V1.2931.00
204_F207_F1.2911.00
278_V286_R1.2861.00
51_M147_A1.2861.00
82_E117_I1.2811.00
295_C386_L1.2761.00
169_Q392_N1.2751.00
207_F360_Q1.2671.00
415_L418_F1.2621.00
173_G176_R1.2591.00
200_L204_F1.2501.00
59_A68_I1.2461.00
342_K374_E1.2371.00
75_A78_A1.2311.00
184_I398_K1.2241.00
87_G121_L1.2181.00
67_T140_K1.2151.00
311_E397_N1.2141.00
167_I211_L1.2141.00
345_G349_T1.2081.00
344_I352_M1.2041.00
179_V182_Q1.1961.00
81_D86_N1.1931.00
49_R84_L1.1861.00
311_E395_T1.1841.00
141_C241_T1.1671.00
254_A260_P1.1651.00
166_A387_L1.1621.00
218_I287_L1.1511.00
336_V382_A1.1511.00
350_V372_M1.1511.00
205_R266_D1.1501.00
181_F398_K1.1491.00
175_E208_S1.1451.00
52_V110_T1.1341.00
52_V83_V1.1331.00
200_L401_C1.1241.00
418_F452_E1.1211.00
141_C242_P1.1181.00
94_P97_V1.1171.00
182_Q201_G1.1101.00
168_N172_E1.1091.00
101_G109_N1.1091.00
298_L333_P1.1071.00
156_V372_M1.1041.00
52_V84_L1.1001.00
170_L211_L1.0961.00
174_F294_I1.0951.00
166_A169_Q1.0901.00
57_A256_V1.0901.00
79_A82_E1.0831.00
340_C344_I1.0831.00
207_F294_I1.0781.00
157_Y259_F1.0761.00
419_I424_G1.0751.00
170_L391_I1.0741.00
164_V168_N1.0731.00
169_Q384_Y1.0731.00
216_K223_E1.0691.00
347_D351_T1.0671.00
82_E88_K1.0671.00
183_D201_G1.0661.00
434_T441_V1.0631.00
159_S162_K1.0611.00
194_D305_P1.0591.00
74_N77_I1.0581.00
197_P405_V1.0561.00
445_R449_T1.0511.00
54_V147_A1.0481.00
64_E256_V1.0441.00
277_Y372_M1.0431.00
162_K380_T1.0401.00
45_P90_M1.0391.00
186_K401_C1.0391.00
75_A79_A1.0351.00
440_E444_E1.0351.00
50_G154_I1.0331.00
152_F369_G1.0331.00
417_P452_E1.0301.00
77_I81_D1.0301.00
184_I401_C1.0271.00
292_S337_N1.0241.00
160_L222_A1.0191.00
177_K204_F1.0191.00
39_N374_E1.0191.00
49_R158_S1.0191.00
177_K391_I1.0181.00
418_F456_I1.0061.00
283_A287_L1.0051.00
428_G432_A1.0041.00
429_K433_E0.9971.00
182_Q199_T0.9971.00
191_Q334_E0.9961.00
209_I265_E0.9951.00
53_M256_V0.9941.00
313_N397_N0.9931.00
198_M235_I0.9921.00
150_T228_V0.9921.00
291_M383_C0.9781.00
317_L389_K0.9761.00
152_F277_Y0.9741.00
75_A134_P0.9731.00
280_V372_M0.9721.00
45_P49_R0.9721.00
220_R279_M0.9711.00
157_Y222_A0.9701.00
70_K73_A0.9701.00
157_Y161_I0.9681.00
300_L359_L0.9681.00
199_T463_M0.9671.00
312_I394_I0.9641.00
209_I213_E0.9561.00
167_I215_V0.9551.00
160_L218_I0.9531.00
298_L394_I0.9511.00
49_R89_C0.9501.00
228_V253_L0.9481.00
65_L242_P0.9451.00
222_A226_L0.9451.00
226_L259_F0.9401.00
54_V150_T0.9351.00
78_A121_L0.9271.00
188_G198_M0.9261.00
81_D84_L0.9251.00
63_K74_N0.9231.00
189_R194_D0.9211.00
89_C117_I0.9131.00
53_M253_L0.9091.00
216_K220_R0.9091.00
69_P137_H0.9071.00
194_D304_G0.9061.00
250_V254_A0.9051.00
425_D429_K0.9011.00
430_I441_V0.8981.00
71_S74_N0.8961.00
46_E158_S0.8941.00
119_L135_N0.8931.00
281_H372_M0.8901.00
281_H347_D0.8881.00
282_G347_D0.8871.00
198_M268_I0.8831.00
405_V459_V0.8801.00
51_M54_V0.8781.00
416_N419_I0.8761.00
171_R175_E0.8751.00
93_F97_V0.8741.00
78_A82_E0.8741.00
61_A249_A0.8741.00
61_A65_L0.8721.00
50_G155_A0.8721.00
97_V101_G0.8681.00
185_L463_M0.8641.00
172_E176_R0.8621.00
174_F204_F0.8621.00
192_L300_L0.8601.00
180_E395_T0.8581.00
184_I396_A0.8571.00
178_A205_R0.8561.00
342_K370_Q0.8541.00
133_N136_D0.8531.00
439_R443_L0.8511.00
203_E360_Q0.8491.00
287_L383_C0.8491.00
308_G400_V0.8481.00
223_E226_L0.8461.00
430_I444_E0.8451.00
174_F208_S0.8451.00
208_S212_K0.8421.00
64_E70_K0.8401.00
439_R450_E0.8381.00
93_F99_Q0.8361.00
115_A118_G0.8351.00
86_N90_M0.8291.00
312_I393_G0.8281.00
173_G177_K0.8261.00
152_F372_M0.8251.00
53_M84_L0.8251.00
292_S355_E0.8231.00
49_R90_M0.8190.99
282_G351_T0.8180.99
174_F391_I0.8150.99
430_I433_E0.8140.99
194_D408_S0.8120.99
452_E456_I0.8120.99
192_L303_S0.8060.99
202_Q267_L0.8040.99
370_Q374_E0.8020.99
336_V386_L0.8010.99
289_V351_T0.8000.99
185_L405_V0.7980.99
346_N374_E0.7960.99
273_D290_K0.7940.99
227_E276_A0.7940.99
58_A142_Q0.7910.99
294_I386_L0.7910.99
87_G117_I0.7870.99
189_R305_P0.7860.99
161_I259_F0.7830.99
59_A73_A0.7810.99
63_K68_I0.7810.99
203_E301_L0.7770.99
181_F396_A0.7750.99
341_F349_T0.7750.99
82_E89_C0.7730.99
72_V134_P0.7720.99
51_M110_T0.7720.99
233_T323_I0.7710.99
213_E227_E0.7640.99
111_N115_A0.7610.99
227_E264_A0.7560.99
263_P267_L0.7550.99
429_K432_A0.7530.99
268_I360_Q0.7530.99
167_I383_C0.7520.99
180_E398_K0.7520.99
229_N270_A0.7510.99
279_M282_G0.7510.99
186_K203_E0.7490.99
353_A367_V0.7480.99
39_N42_S0.7470.99
426_I430_I0.7470.99
315_P393_G0.7440.99
344_I348_T0.7440.99
76_I134_P0.7440.99
211_L383_C0.7420.99
80_C83_V0.7420.99
342_K346_N0.7390.99
183_D460_Q0.7370.99
349_T371_A0.7370.99
445_R448_L0.7370.99
451_A455_D0.7360.99
60_M64_E0.7340.99
406_Y439_R0.7340.99
160_L221_T0.7320.99
390_C394_I0.7300.99
301_L359_L0.7300.99
280_V284_L0.7280.99
313_N316_E0.7270.99
275_G290_K0.7250.99
346_N376_V0.7230.99
184_I199_T0.7220.99
453_L457_F0.7190.99
87_G92_Q0.7160.99
211_L214_E0.7160.99
68_I140_K0.7140.99
250_V263_P0.7130.99
72_V76_I0.7120.99
47_F148_Y0.7120.99
206_A293_K0.7120.99
272_S360_Q0.7120.99
313_N393_G0.7110.99
172_E175_E0.7100.99
56_K256_V0.7090.99
295_C333_P0.7080.99
83_V114_L0.7050.98
56_K73_A0.7050.98
226_L254_A0.7030.98
120_E123_G0.7020.98
163_L376_V0.7010.98
64_E242_P0.7000.98
97_V100_G0.7000.98
43_D370_Q0.6980.98
210_L290_K0.6970.98
99_Q113_V0.6970.98
187_M307_A0.6960.98
308_G397_N0.6960.98
269_E275_G0.6930.98
262_V265_E0.6910.98
448_L456_I0.6900.98
83_V93_F0.6890.98
253_L256_V0.6890.98
174_F211_L0.6870.98
217_N275_G0.6870.98
93_F100_G0.6850.98
162_K166_A0.6830.98
372_M376_V0.6820.98
436_K442_V0.6820.98
396_A401_C0.6810.98
230_L253_L0.6800.98
342_K378_I0.6790.98
341_F353_A0.6790.98
67_T242_P0.6790.98
419_I423_N0.6780.98
71_S75_A0.6760.98
383_C387_L0.6750.98
280_V377_H0.6740.98
68_I72_V0.6740.98
153_R276_A0.6740.98
107_N315_P0.6720.98
157_Y260_P0.6700.98
176_R179_V0.6700.98
278_V350_V0.6700.98
294_I391_I0.6690.98
168_N212_K0.6680.98
79_A122_M0.6670.98
68_I73_A0.6650.98
304_G308_G0.6640.98
163_L380_T0.6630.98
94_P99_Q0.6630.98
441_V444_E0.6620.98
57_A60_M0.6620.98
460_Q464_H0.6610.98
399_E402_E0.6600.98
277_Y350_V0.6600.98
42_S159_S0.6590.98
437_S441_V0.6580.98
210_L273_D0.6540.98
184_I402_E0.6540.98
186_K198_M0.6540.98
296_N337_N0.6510.97
214_E286_R0.6510.97
420_G423_N0.6490.97
69_P73_A0.6470.97
134_P137_H0.6470.97
65_L252_K0.6460.97
181_F200_L0.6460.97
168_N171_R0.6440.97
169_Q173_G0.6440.97
344_I351_T0.6420.97
187_M405_V0.6420.97
291_M387_L0.6410.97
179_V183_D0.6410.97
59_A76_I0.6400.97
440_E450_E0.6390.97
426_I433_E0.6390.97
39_N370_Q0.6390.97
63_K77_I0.6380.97
292_S352_M0.6380.97
170_L387_L0.6370.97
341_F352_M0.6370.97
349_T353_A0.6360.97
96_D99_Q0.6360.97
441_V445_R0.6360.97
204_F401_C0.6350.97
119_L123_G0.6350.97
269_E290_K0.6350.97
89_C92_Q0.6350.97
278_V351_T0.6340.97
133_N422_H0.6340.97
41_I46_E0.6340.97
221_T225_L0.6330.97
216_K219_Q0.6300.97
400_V407_N0.6290.97
278_V282_G0.6280.97
178_A182_Q0.6270.97
155_A376_V0.6260.97
163_L284_L0.6230.97
38_N363_V0.6210.97
108_M111_N0.6180.97
221_T280_V0.6180.97
67_T141_C0.6180.97
93_F101_G0.6170.97
317_L393_G0.6150.96
86_N89_C0.6140.96
60_M73_A0.6130.96
443_L454_D0.6130.96
280_V376_V0.6130.96
160_L225_L0.6120.96
177_K394_I0.6100.96
217_N224_L0.6080.96
68_I76_I0.6080.96
287_L379_L0.6050.96
161_I219_Q0.6050.96
52_V90_M0.6050.96
332_V386_L0.6040.96
116_N120_E0.6030.96
336_V390_C0.6020.96
159_S376_V0.6020.96
404_Y407_N0.6010.96
59_A138_V0.6010.96
217_N220_R0.6010.96
214_E217_N0.6000.96
82_E87_G0.6000.96
170_L383_C0.5990.96
428_G431_C0.5990.96
406_Y437_S0.5990.96
210_L272_S0.5970.96
160_L164_V0.5970.96
310_N397_N0.5970.96
440_E443_L0.5940.96
284_L379_L0.5940.96
72_V75_A0.5920.96
349_T368_I0.5910.96
348_T351_T0.5910.96
430_I436_K0.5860.95
249_A253_L0.5850.95
405_V410_G0.5850.95
163_L379_L0.5830.95
137_H140_K0.5820.95
218_I283_A0.5800.95
411_I414_Y0.5800.95
288_A379_L0.5780.95
426_I445_R0.5780.95
279_M286_R0.5780.95
371_A374_E0.5780.95
398_K401_C0.5770.95
228_V263_P0.5760.95
216_K227_E0.5740.95
165_D169_Q0.5740.95
337_N364_M0.5730.95
332_V390_C0.5720.95
175_E178_A0.5720.95
285_K289_V0.5720.95
54_V151_G0.5710.95
443_L455_D0.5710.95
345_G348_T0.5690.95
461_N464_H0.5690.95
194_D410_G0.5660.94
421_H425_D0.5650.94
207_F210_L0.5640.94
111_N114_L0.5610.94
212_K216_K0.5590.94
170_L173_G0.5580.94
53_M257_T0.5570.94
284_L287_L0.5560.94
184_I201_G0.5540.94
203_E297_D0.5540.94
88_K91_D0.5540.94
318_Q331_V0.5530.94
286_R354_A0.5510.94
298_L386_L0.5510.94
455_D461_N0.5490.93
59_A77_I0.5490.93
100_G109_N0.5460.93
211_L291_M0.5450.93
186_K301_L0.5440.93
369_G373_F0.5420.93
87_G90_M0.5420.93
82_E86_N0.5410.93
50_G53_M0.5410.93
204_F394_I0.5400.93
167_I218_I0.5380.93
223_E227_E0.5370.93
211_L287_L0.5360.93
282_G285_K0.5360.93
199_T464_H0.5360.93
219_Q223_E0.5350.93
336_V340_C0.5350.93
339_V378_I0.5340.93
164_V283_A0.5330.92
227_E265_E0.5330.92
455_D458_S0.5330.92
53_M57_A0.5320.92
192_L358_Q0.5320.92
221_T279_M0.5320.92
156_V225_L0.5310.92
428_G433_E0.5300.92
217_N286_R0.5290.92
54_V230_L0.5290.92
166_A385_N0.5280.92
209_I212_K0.5270.92
191_Q363_V0.5260.92
249_A252_K0.5250.92
111_N118_G0.5240.92
144_T229_N0.5240.92
58_A253_L0.5230.92
295_C337_N0.5230.92
298_L391_I0.5230.92
52_V80_C0.5230.92
292_S356_A0.5220.92
99_Q116_N0.5220.92
115_A119_L0.5190.91
415_L453_L0.5180.91
38_N332_V0.5180.91
422_H426_I0.5160.91
198_M202_Q0.5160.91
48_V88_K0.5150.91
343_V380_T0.5140.91
229_N271_T0.5130.91
284_L343_V0.5130.91
181_F201_G0.5120.91
157_Y225_L0.5120.91
387_L391_I0.5120.91
185_L459_V0.5110.91
46_E162_K0.5110.91
158_S161_I0.5100.91
116_N123_G0.5100.91
300_L303_S0.5100.91
83_V90_M0.5090.91
281_H375_S0.5070.90
275_G279_M0.5060.90
95_V99_Q0.5060.90
84_L89_C0.5040.90
403_G406_Y0.5040.90
427_V442_V0.5020.90
234_A323_I0.5020.90
52_V87_G0.5000.90
244_E255_E0.5000.90
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1yfmA 4 0.9393 100 0.145 Contact Map
1furA 4 0.9582 100 0.147 Contact Map
1vdkA 4 0.9603 100 0.155 Contact Map
1jswA 4 0.9623 100 0.158 Contact Map
3e04A 4 0.9644 100 0.16 Contact Map
3r6qA 4 0.9686 100 0.16 Contact Map
3gtdA 4 0.9498 100 0.161 Contact Map
4hgvA 4 0.9623 100 0.166 Contact Map
3ocfA 4 0.9393 100 0.175 Contact Map
4admA 4 0.9477 100 0.181 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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