GREMLIN Database
FLGB - Flagellar basal body rod protein FlgB
UniProt: P0ABW9 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG14266
Length: 138 (125)
Sequences: 1540 (1190)
Seq/√Len: 106.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
68_T72_H2.9891.00
65_L75_A2.9601.00
30_I102_M2.9571.00
25_V43_I2.8501.00
48_E52_V2.8191.00
9_L127_I2.6271.00
44_D47_S2.6211.00
48_E51_K2.6171.00
38_Y41_R2.6011.00
14_E17_N2.5661.00
19_R112_N2.5501.00
125_G129_G2.4651.00
108_Q111_D2.4541.00
111_D115_Q2.3981.00
18_L43_I2.3461.00
67_M74_P2.3001.00
21_Q24_E2.1691.00
25_V85_L2.1591.00
45_F49_L1.9891.00
28_A38_Y1.9841.00
40_A99_T1.9621.00
22_R42_D1.9171.00
128_K131_M1.8551.00
21_Q43_I1.8541.00
67_M73_I1.8411.00
46_A50_K1.7381.00
130_M134_L1.7221.00
53_M56_G1.6591.00
23_Q112_N1.5571.00
12_Q119_S1.5411.00
26_L42_D1.5081.00
26_L109_F1.4661.00
132_N135_Q1.4241.00
117_Q120_L1.4161.00
121_S125_G1.3471.00
116_Y120_L1.3410.99
41_R87_Y1.3040.99
101_D104_R1.2920.99
120_L124_S1.2880.99
102_M106_R1.2640.99
13_Q17_N1.1970.99
24_E27_A1.1870.99
104_R107_T1.1780.99
28_A120_L1.1750.99
104_R108_Q1.1690.99
131_M135_Q1.1440.98
90_P101_D1.1370.98
106_R110_A1.1310.98
126_Q129_G1.1090.98
8_A13_Q1.0940.98
23_Q103_D1.0700.97
16_L20_A1.0570.97
28_A41_R1.0550.97
30_I33_A1.0490.97
34_D92_Q1.0460.97
26_L112_N0.9980.96
103_D106_R0.9970.96
65_L72_H0.9940.96
40_A88_R0.9860.96
25_V38_Y0.9660.95
41_R88_R0.9640.95
69_S72_H0.9440.95
66_T114_L0.9390.95
15_A118_M0.9270.94
18_L25_V0.9080.94
11_F49_L0.8910.93
17_N20_A0.8810.93
42_D47_S0.8790.92
93_P104_R0.8730.92
19_R115_Q0.8550.91
9_L126_Q0.8510.91
12_Q122_A0.8430.91
43_I86_Q0.8400.91
23_Q109_F0.8310.90
91_D99_T0.8300.90
50_K54_Q0.8260.90
64_A78_L0.8200.90
95_L107_T0.8160.89
22_R104_R0.8150.89
117_Q124_S0.8140.89
18_L22_R0.8100.89
19_R28_A0.8060.89
15_A115_Q0.8020.89
71_Q77_A0.7960.88
74_P78_L0.7940.88
122_A129_G0.7880.88
9_L36_P0.7870.88
44_D86_Q0.7810.87
49_L53_M0.7770.87
22_R108_Q0.7760.87
121_S127_I0.7710.87
30_I111_D0.7700.86
65_L73_I0.7670.86
64_A74_P0.7670.86
72_H106_R0.7600.86
118_M122_A0.7580.86
48_E117_Q0.7510.85
18_L99_T0.7500.85
12_Q27_A0.7470.85
24_E117_Q0.7410.84
16_L120_L0.7390.84
19_R101_D0.7390.84
12_Q126_Q0.7330.84
10_R23_Q0.7220.83
91_D104_R0.7200.83
64_A82_T0.7190.82
119_S123_L0.7160.82
31_A90_P0.7030.81
93_P101_D0.7000.81
106_R109_F0.6990.81
11_F102_M0.6910.80
121_S124_S0.6820.79
63_V78_L0.6780.79
13_Q34_D0.6680.78
21_Q83_A0.6680.78
102_M125_G0.6620.77
38_Y86_Q0.6570.76
63_V79_T0.6510.76
127_I130_M0.6470.75
9_L13_Q0.6450.75
78_L83_A0.6440.75
14_E114_L0.6360.74
107_T129_G0.6310.74
42_D120_L0.6270.73
70_T78_L0.6270.73
95_L135_Q0.6180.72
118_M125_G0.6180.72
27_A122_A0.6150.72
49_L52_V0.6120.71
15_A19_R0.6100.71
110_A113_S0.6050.70
63_V121_S0.6010.70
123_L135_Q0.6000.70
71_Q75_A0.5990.70
127_I134_L0.5950.69
107_T110_A0.5920.69
123_L126_Q0.5790.67
123_L127_I0.5730.66
47_S78_L0.5730.66
26_L90_P0.5700.66
71_Q79_T0.5700.66
25_V41_R0.5700.66
69_S73_I0.5690.66
128_K132_N0.5680.66
42_D86_Q0.5680.66
55_R63_V0.5680.66
122_A126_Q0.5640.65
67_M107_T0.5630.65
40_A105_E0.5620.65
91_D101_D0.5610.65
30_I132_N0.5600.65
16_L68_T0.5580.64
20_A136_S0.5570.64
41_R90_P0.5570.64
73_I77_A0.5490.63
39_Q127_I0.5480.63
12_Q15_A0.5450.62
54_Q64_A0.5440.62
42_D88_R0.5410.62
11_F127_I0.5380.61
50_K53_M0.5370.61
27_A97_G0.5340.61
55_R82_T0.5340.61
98_N136_S0.5330.61
117_Q121_S0.5320.61
94_S130_M0.5320.61
64_A77_A0.5300.60
6_D10_R0.5300.60
55_R76_Q0.5290.60
13_Q102_M0.5280.60
47_S50_K0.5280.60
95_L104_R0.5260.60
104_R123_L0.5240.59
6_D117_Q0.5230.59
117_Q125_G0.5210.59
67_M72_H0.5210.59
8_A12_Q0.5190.59
37_G80_P0.5180.59
12_Q77_A0.5180.59
45_F101_D0.5170.58
12_Q16_L0.5170.58
10_R49_L0.5080.57
90_P93_P0.5070.57
94_S99_T0.5030.56
47_S51_K0.5030.56
74_P79_T0.5000.56
38_Y56_G0.5000.56
77_A82_T0.5000.56
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3a69A 3 0.6232 98.6 0.764 Contact Map
4ut1A 1 0.2029 98.2 0.788 Contact Map
1oryB 2 0.2681 8.4 0.943 Contact Map
3buxB 1 0.6739 7.5 0.944 Contact Map
4ea3A 2 0.2681 6.6 0.945 Contact Map
3kx6A 4 0.8043 5.3 0.948 Contact Map
4d2jA 4 0.8043 5.1 0.948 Contact Map
2d4yA 1 0.6014 4.3 0.95 Contact Map
3zey3 1 0.3913 4.1 0.95 Contact Map
2bzwB 1 0.0725 3.6 0.952 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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